Capybara microvirus Cap1_SP_263

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W5I9|A0A4P8W5I9_9VIRU Replication initiator protein OS=Capybara microvirus Cap1_SP_263 OX=2585420 PE=4 SV=1
MM1 pKa = 7.65LNFLNFYY8 pKa = 10.72VKK10 pKa = 10.22FFFIMKK16 pKa = 9.64SISKK20 pKa = 9.06FHH22 pKa = 5.97YY23 pKa = 8.51NCKK26 pKa = 7.88KK27 pKa = 10.16QKK29 pKa = 10.27YY30 pKa = 7.9EE31 pKa = 4.12VNSGEE36 pKa = 4.31IKK38 pKa = 10.65VEE40 pKa = 3.9PCSCLSIKK48 pKa = 10.64DD49 pKa = 3.0IFEE52 pKa = 4.2RR53 pKa = 11.84FRR55 pKa = 11.84LGLPLDD61 pKa = 3.78SSVCINDD68 pKa = 3.68SYY70 pKa = 11.96DD71 pKa = 3.69EE72 pKa = 4.25EE73 pKa = 5.47SSTFLDD79 pKa = 3.3INTIKK84 pKa = 10.81QPDD87 pKa = 4.21LVSKK91 pKa = 10.42IDD93 pKa = 3.81LVASLHH99 pKa = 5.92EE100 pKa = 4.63FNKK103 pKa = 10.42SASEE107 pKa = 4.14TSVSRR112 pKa = 11.84SEE114 pKa = 3.97MGVEE118 pKa = 4.16EE119 pKa = 4.1VQPLNEE125 pKa = 4.19SAEE128 pKa = 4.03EE129 pKa = 3.82SDD131 pKa = 5.07NIEE134 pKa = 4.05KK135 pKa = 10.77SS136 pKa = 3.26

Molecular weight: 15.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W5R5|A0A4P8W5R5_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_263 OX=2585420 PE=3 SV=1
MM1 pKa = 7.68CLFPKK6 pKa = 9.85QVRR9 pKa = 11.84VTLKK13 pKa = 10.43DD14 pKa = 3.35GHH16 pKa = 5.92SVPMSVPCGKK26 pKa = 10.67CIDD29 pKa = 4.01CLQLRR34 pKa = 11.84THH36 pKa = 6.43DD37 pKa = 3.04WEE39 pKa = 4.76LRR41 pKa = 11.84MKK43 pKa = 11.09YY44 pKa = 10.07EE45 pKa = 4.6LLNSNYY51 pKa = 10.54GLFVTLTYY59 pKa = 11.25DD60 pKa = 4.44DD61 pKa = 5.63FSCPSLGVEE70 pKa = 4.15VSHH73 pKa = 5.4VQKK76 pKa = 10.72FIKK79 pKa = 10.07RR80 pKa = 11.84LRR82 pKa = 11.84KK83 pKa = 8.97HH84 pKa = 5.05GHH86 pKa = 4.56IFKK89 pKa = 9.97YY90 pKa = 10.46YY91 pKa = 10.49CIGEE95 pKa = 4.37YY96 pKa = 9.42GTSTFRR102 pKa = 11.84PHH104 pKa = 4.74YY105 pKa = 9.77HH106 pKa = 7.12LIFFVTSDD114 pKa = 3.12SHH116 pKa = 8.29SINLSLDD123 pKa = 3.38INKK126 pKa = 9.44SWPYY130 pKa = 11.03GFTQVKK136 pKa = 8.43PCSFGRR142 pKa = 11.84IHH144 pKa = 6.46YY145 pKa = 9.47CIAYY149 pKa = 10.33LIGQKK154 pKa = 10.39SPLGKK159 pKa = 10.1NKK161 pKa = 9.78PFTIMSKK168 pKa = 10.07RR169 pKa = 11.84PSIGLCALDD178 pKa = 4.7DD179 pKa = 3.87KK180 pKa = 11.46TFVSQLKK187 pKa = 8.67STNFEE192 pKa = 3.96YY193 pKa = 10.61TFRR196 pKa = 11.84NGHH199 pKa = 5.73KK200 pKa = 9.54VHH202 pKa = 5.84VPRR205 pKa = 11.84YY206 pKa = 8.39YY207 pKa = 10.19RR208 pKa = 11.84RR209 pKa = 11.84KK210 pKa = 10.57LNDD213 pKa = 3.34KK214 pKa = 10.69DD215 pKa = 3.48FTLIDD220 pKa = 4.67FYY222 pKa = 11.56LLKK225 pKa = 10.44CKK227 pKa = 10.16HH228 pKa = 6.37HH229 pKa = 5.78EE230 pKa = 4.28QFLKK234 pKa = 10.53DD235 pKa = 3.5YY236 pKa = 10.97KK237 pKa = 11.03SYY239 pKa = 11.4LNSKK243 pKa = 9.75HH244 pKa = 6.8LDD246 pKa = 3.55FSKK249 pKa = 10.49HH250 pKa = 4.84YY251 pKa = 11.15NNLDD255 pKa = 3.84LIKK258 pKa = 10.34DD259 pKa = 4.02FKK261 pKa = 10.71ISQSIYY267 pKa = 10.44NFSHH271 pKa = 7.83DD272 pKa = 4.57EE273 pKa = 3.73ILKK276 pKa = 10.06NRR278 pKa = 11.84NKK280 pKa = 10.38KK281 pKa = 9.94LKK283 pKa = 10.46NDD285 pKa = 3.41

Molecular weight: 33.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1315

136

531

328.8

37.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.411 ± 1.508

1.369 ± 0.806

5.779 ± 0.637

6.008 ± 1.365

6.236 ± 1.05

5.551 ± 1.26

2.586 ± 0.91

5.399 ± 0.474

6.996 ± 1.589

10.038 ± 0.901

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.293 ± 0.196

7.605 ± 1.151

3.878 ± 0.599

5.095 ± 1.323

4.487 ± 0.881

8.061 ± 1.399

4.487 ± 0.457

4.867 ± 0.698

0.913 ± 0.226

4.943 ± 0.536
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski