Thalassomonas phage BA3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8HP74|A8HP74_9CAUD Uncharacterized protein OS=Thalassomonas phage BA3 OX=469660 GN=BA3_0041 PE=4 SV=1
MM1 pKa = 6.83NTEE4 pKa = 4.01EE5 pKa = 4.28LQIEE9 pKa = 4.31ILGKK13 pKa = 10.0EE14 pKa = 3.73IEE16 pKa = 4.45FQVEE20 pKa = 4.01FNFTKK25 pKa = 9.69GTPGVYY31 pKa = 10.0HH32 pKa = 7.21LAPEE36 pKa = 4.66DD37 pKa = 3.9CCPAEE42 pKa = 3.98PDD44 pKa = 3.6EE45 pKa = 4.85YY46 pKa = 10.64EE47 pKa = 4.23VCALYY52 pKa = 10.75CVGYY56 pKa = 9.63EE57 pKa = 3.69QGKK60 pKa = 9.74RR61 pKa = 11.84KK62 pKa = 10.12LYY64 pKa = 10.12DD65 pKa = 3.05ASYY68 pKa = 11.32LLDD71 pKa = 3.97EE72 pKa = 5.04LSEE75 pKa = 4.38EE76 pKa = 4.78IISEE80 pKa = 4.19LL81 pKa = 4.19

Molecular weight: 9.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8HP82|A8HP82_9CAUD Uncharacterized protein OS=Thalassomonas phage BA3 OX=469660 GN=BA3_0044 PE=4 SV=1
MM1 pKa = 7.97IYY3 pKa = 10.1PLYY6 pKa = 9.85YY7 pKa = 10.04HH8 pKa = 6.85GRR10 pKa = 11.84SKK12 pKa = 10.13TNSDD16 pKa = 2.59KK17 pKa = 11.54DD18 pKa = 3.92FILRR22 pKa = 11.84RR23 pKa = 11.84MAVIPEE29 pKa = 3.99QYY31 pKa = 8.4QQRR34 pKa = 11.84VADD37 pKa = 3.64KK38 pKa = 9.29YY39 pKa = 10.39EE40 pKa = 4.53RR41 pKa = 11.84IFRR44 pKa = 11.84SRR46 pKa = 11.84QTGFRR51 pKa = 11.84KK52 pKa = 9.64NANTYY57 pKa = 7.43LHH59 pKa = 7.0RR60 pKa = 11.84VACWFRR66 pKa = 11.84YY67 pKa = 9.14RR68 pKa = 11.84RR69 pKa = 11.84NKK71 pKa = 10.02KK72 pKa = 9.2QEE74 pKa = 3.6QSIEE78 pKa = 4.3KK79 pKa = 9.51IRR81 pKa = 11.84KK82 pKa = 5.68RR83 pKa = 11.84TSS85 pKa = 2.53

Molecular weight: 10.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

12339

47

2631

262.5

29.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.372 ± 0.417

0.835 ± 0.206

5.843 ± 0.302

8.356 ± 0.537

3.914 ± 0.253

6.727 ± 0.416

1.491 ± 0.213

6.111 ± 0.284

8.096 ± 0.486

8.023 ± 0.4

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.423 ± 0.143

5.138 ± 0.346

3.671 ± 0.252

4.717 ± 0.325

4.733 ± 0.273

6.005 ± 0.453

5.495 ± 0.268

5.738 ± 0.239

1.135 ± 0.156

3.177 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski