Nicotiana tabacum (Common tobacco)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae; Nicotiana

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73607 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S3X1K5|A0A1S3X1K5_TOBAC protein Hook homolog OS=Nicotiana tabacum OX=4097 GN=LOC107760066 PE=4 SV=1
MM1 pKa = 7.2RR2 pKa = 11.84RR3 pKa = 11.84KK4 pKa = 9.65VRR6 pKa = 11.84SLSDD10 pKa = 3.36LYY12 pKa = 11.18RR13 pKa = 11.84RR14 pKa = 11.84IPKK17 pKa = 9.66CASSSVSADD26 pKa = 3.43PQSPSINISAAQAGTGTEE44 pKa = 4.22VATTEE49 pKa = 4.01QLTPEE54 pKa = 3.98MDD56 pKa = 3.8LPIEE60 pKa = 4.39LDD62 pKa = 3.42LPAFFTQADD71 pKa = 4.32FPVLPSFEE79 pKa = 4.23QTTDD83 pKa = 3.26PFDD86 pKa = 4.93DD87 pKa = 4.69LFDD90 pKa = 5.19NICEE94 pKa = 3.98QIIAIVQPFPEE105 pKa = 4.21FQGWDD110 pKa = 3.63LYY112 pKa = 11.42FLDD115 pKa = 4.53EE116 pKa = 4.83VISLSLFSLMDD127 pKa = 3.54EE128 pKa = 4.63YY129 pKa = 11.55YY130 pKa = 10.84FSIEE134 pKa = 4.13CLQGIYY140 pKa = 10.45LDD142 pKa = 5.59LITNGASSAFLTFFLNYY159 pKa = 10.27

Molecular weight: 17.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5MA29|Q5MA29_TOBAC Reverse transcriptase Ty1/copia-type domain-containing protein OS=Nicotiana tabacum OX=4097 GN=orf152 PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.27WKK6 pKa = 9.53KK7 pKa = 9.88KK8 pKa = 8.64RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 8.21RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.46MRR21 pKa = 11.84QRR23 pKa = 11.84SKK25 pKa = 11.41

Molecular weight: 3.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61676

11931

73607

29996979

7

4876

407.5

45.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.578 ± 0.008

1.858 ± 0.004

5.288 ± 0.006

6.649 ± 0.015

4.202 ± 0.007

6.249 ± 0.008

2.353 ± 0.004

5.5 ± 0.006

6.436 ± 0.01

9.631 ± 0.012

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.429 ± 0.004

4.627 ± 0.006

4.8 ± 0.009

3.799 ± 0.007

5.2 ± 0.006

8.888 ± 0.011

4.921 ± 0.004

6.437 ± 0.007

1.265 ± 0.004

2.887 ± 0.005
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski