Aphis craccivora (Cowpea aphid)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Paraneoptera; Hemiptera; Sternorrhyncha; Aphidomorpha; Aphidoidea; Aphididae;

Average proteome isoelectric point is 7.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31792 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G0Z530|A0A6G0Z530_APHCR Frizzled-2-like OS=Aphis craccivora OX=307492 GN=FWK35_00013649 PE=3 SV=1
MM1 pKa = 7.58DD2 pKa = 3.77THH4 pKa = 8.2ASFIEE9 pKa = 3.98EE10 pKa = 4.54DD11 pKa = 3.47EE12 pKa = 4.51TIQRR16 pKa = 11.84CEE18 pKa = 4.06SNITLIVDD26 pKa = 4.69EE27 pKa = 4.36ITFGKK32 pKa = 8.29GTLYY36 pKa = 10.56VAEE39 pKa = 4.74SKK41 pKa = 10.92LYY43 pKa = 7.72WKK45 pKa = 10.38NYY47 pKa = 9.82AISQVVSIDD56 pKa = 3.71YY57 pKa = 11.04KK58 pKa = 10.1SMCVFGTCNHH68 pKa = 6.38PVVHH72 pKa = 6.82EE73 pKa = 4.57KK74 pKa = 10.57PCLQIIVDD82 pKa = 4.25FAYY85 pKa = 9.84KK86 pKa = 9.67PDD88 pKa = 4.42SIQSEE93 pKa = 4.16NGQTLNGDD101 pKa = 3.32NHH103 pKa = 7.67SIEE106 pKa = 5.75DD107 pKa = 4.36DD108 pKa = 4.54DD109 pKa = 5.84NSEE112 pKa = 4.87DD113 pKa = 5.19DD114 pKa = 4.97NEE116 pKa = 4.03VDD118 pKa = 3.48QNEE121 pKa = 4.27EE122 pKa = 3.91EE123 pKa = 4.2EE124 pKa = 4.72EE125 pKa = 4.14EE126 pKa = 4.2MKK128 pKa = 11.02SKK130 pKa = 10.61IKK132 pKa = 10.41LVPDD136 pKa = 3.4SPEE139 pKa = 4.02CLNEE143 pKa = 3.56IYY145 pKa = 10.49EE146 pKa = 4.4AFTRR150 pKa = 11.84VQPLHH155 pKa = 6.43NSNDD159 pKa = 3.42EE160 pKa = 4.2DD161 pKa = 4.31SEE163 pKa = 4.53EE164 pKa = 4.05EE165 pKa = 4.48AEE167 pKa = 4.34EE168 pKa = 4.52EE169 pKa = 4.38DD170 pKa = 4.03DD171 pKa = 5.52FYY173 pKa = 11.92YY174 pKa = 11.13NEE176 pKa = 3.85NDD178 pKa = 3.18EE179 pKa = 4.86FEE181 pKa = 6.16DD182 pKa = 4.38YY183 pKa = 11.4DD184 pKa = 4.59EE185 pKa = 6.31DD186 pKa = 4.83DD187 pKa = 3.98AGQILRR193 pKa = 11.84NN194 pKa = 3.73

Molecular weight: 22.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G0ZP15|A0A6G0ZP15_APHCR THAP-type domain-containing protein OS=Aphis craccivora OX=307492 GN=FWK35_00004576 PE=4 SV=1
MM1 pKa = 6.94MRR3 pKa = 11.84IRR5 pKa = 11.84NVNVLIRR12 pKa = 11.84NQMKK16 pKa = 10.26KK17 pKa = 10.03AAKK20 pKa = 9.41KK21 pKa = 9.48RR22 pKa = 11.84KK23 pKa = 8.05KK24 pKa = 10.05KK25 pKa = 10.15KK26 pKa = 9.95RR27 pKa = 11.84NLHH30 pKa = 5.43LAHH33 pKa = 7.0RR34 pKa = 11.84PVKK37 pKa = 10.16LIKK40 pKa = 10.22RR41 pKa = 11.84ISKK44 pKa = 9.75II45 pKa = 3.39

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

31735

57

31792

10276363

8

7968

323.2

36.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.893 ± 0.016

2.179 ± 0.011

5.312 ± 0.011

5.892 ± 0.015

4.372 ± 0.013

4.554 ± 0.021

2.486 ± 0.008

7.128 ± 0.016

7.572 ± 0.022

9.179 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.328 ± 0.006

6.516 ± 0.017

4.336 ± 0.015

3.917 ± 0.014

4.728 ± 0.015

8.156 ± 0.018

5.946 ± 0.012

5.885 ± 0.012

1.086 ± 0.005

3.531 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski