Circovirus-like genome SAR-B

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 8.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6GIJ3|C6GIJ3_9VIRU Putative Rep protein OS=Circovirus-like genome SAR-B OX=642259 PE=4 SV=1
MM1 pKa = 7.78ASQHH5 pKa = 5.09GWVFTLNNPSIEE17 pKa = 4.12EE18 pKa = 4.1YY19 pKa = 9.82PLKK22 pKa = 10.78FEE24 pKa = 4.33EE25 pKa = 4.6TSSGRR30 pKa = 11.84RR31 pKa = 11.84YY32 pKa = 9.98PIAKK36 pKa = 9.15WEE38 pKa = 4.15LPRR41 pKa = 11.84ATLAFLGCGRR51 pKa = 11.84EE52 pKa = 3.99RR53 pKa = 11.84GQSNTPHH60 pKa = 6.27LQGLLISSRR69 pKa = 11.84PVSLKK74 pKa = 10.31SLKK77 pKa = 10.23KK78 pKa = 10.32LNPRR82 pKa = 11.84AHH84 pKa = 6.73WEE86 pKa = 3.79PMRR89 pKa = 11.84GSFKK93 pKa = 10.22QARR96 pKa = 11.84EE97 pKa = 3.94YY98 pKa = 10.97CEE100 pKa = 4.7KK101 pKa = 10.57EE102 pKa = 4.05GKK104 pKa = 8.93FVQWTKK110 pKa = 10.64SGEE113 pKa = 4.46SVASLSRR120 pKa = 11.84YY121 pKa = 7.97VQTDD125 pKa = 3.3LEE127 pKa = 4.53IFQMIQQSDD136 pKa = 3.33TDD138 pKa = 4.29LNKK141 pKa = 10.71LSVTMLKK148 pKa = 9.75QEE150 pKa = 4.19KK151 pKa = 10.04KK152 pKa = 10.36LAEE155 pKa = 3.84LSKK158 pKa = 10.65RR159 pKa = 11.84IEE161 pKa = 4.79DD162 pKa = 3.6LTKK165 pKa = 10.62LQSHH169 pKa = 7.18FLLKK173 pKa = 10.05QDD175 pKa = 3.81NFQNSILKK183 pKa = 10.22LLSQKK188 pKa = 10.63KK189 pKa = 9.61ILNTLSDD196 pKa = 3.6EE197 pKa = 4.37DD198 pKa = 5.01LII200 pKa = 5.98

Molecular weight: 23.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6GIJ3|C6GIJ3_9VIRU Putative Rep protein OS=Circovirus-like genome SAR-B OX=642259 PE=4 SV=1
MM1 pKa = 8.04VRR3 pKa = 11.84MTTGRR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 8.12TFRR13 pKa = 11.84SIKK16 pKa = 10.09KK17 pKa = 8.8VATRR21 pKa = 11.84GKK23 pKa = 10.36DD24 pKa = 3.3VIDD27 pKa = 3.74TVIEE31 pKa = 3.88ATEE34 pKa = 3.97KK35 pKa = 10.91GKK37 pKa = 10.78EE38 pKa = 3.95FVDD41 pKa = 5.14NISQMPVKK49 pKa = 10.37EE50 pKa = 4.3VMTTAAKK57 pKa = 10.14LAQSKK62 pKa = 10.04KK63 pKa = 10.29SPIGATGLNLLPKK76 pKa = 9.19EE77 pKa = 4.06ARR79 pKa = 11.84SVVSSTDD86 pKa = 3.42AIGDD90 pKa = 3.92FTSSASMFMYY100 pKa = 10.43RR101 pKa = 11.84PPRR104 pKa = 11.84KK105 pKa = 9.54SNRR108 pKa = 11.84AGFTKK113 pKa = 10.73YY114 pKa = 9.07QLKK117 pKa = 9.61TGATRR122 pKa = 11.84TNSSLSNQVGTSDD135 pKa = 4.2MNILDD140 pKa = 5.32AIQVEE145 pKa = 4.57NNPDD149 pKa = 3.26TDD151 pKa = 3.49AKK153 pKa = 10.86YY154 pKa = 11.21SNLSVKK160 pKa = 10.02EE161 pKa = 3.87AFDD164 pKa = 3.99NYY166 pKa = 10.83LLAAGLKK173 pKa = 8.53DD174 pKa = 3.71TAGTSYY180 pKa = 10.66DD181 pKa = 3.42QKK183 pKa = 10.58IQQTSIHH190 pKa = 5.99VSSLQSEE197 pKa = 4.17LVITNNNNDD206 pKa = 3.32TVMVDD211 pKa = 3.65LYY213 pKa = 11.18EE214 pKa = 4.93LVPQHH219 pKa = 6.09TLGPSQYY226 pKa = 10.54VNEE229 pKa = 4.27NQATGYY235 pKa = 8.37MSPVWTWTQGLSDD248 pKa = 3.73TVMVDD253 pKa = 3.41DD254 pKa = 5.04ALSRR258 pKa = 11.84SYY260 pKa = 10.8FQANPFNSSLFSRR273 pKa = 11.84TWKK276 pKa = 9.86IVKK279 pKa = 8.45QVRR282 pKa = 11.84ANISGGSTHH291 pKa = 5.75RR292 pKa = 11.84HH293 pKa = 4.87KK294 pKa = 10.68SAYY297 pKa = 8.92MINKK301 pKa = 6.71TVSYY305 pKa = 10.82PEE307 pKa = 3.85MAQFTTRR314 pKa = 11.84GGKK317 pKa = 8.71FAGWNPTFMIVQQGVPTSAAQEE339 pKa = 4.4GVASSITVRR348 pKa = 11.84MNAQLNYY355 pKa = 9.01EE356 pKa = 4.21ASAVEE361 pKa = 3.86QARR364 pKa = 11.84VIVFDD369 pKa = 4.05SKK371 pKa = 9.86TT372 pKa = 3.19

Molecular weight: 40.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

572

200

372

286.0

31.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.818 ± 1.505

0.35 ± 0.347

4.371 ± 0.465

5.245 ± 1.471

3.671 ± 0.176

5.245 ± 0.398

1.399 ± 0.321

4.545 ± 0.024

7.517 ± 1.059

8.392 ± 2.995

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.147 ± 0.612

5.42 ± 0.758

3.671 ± 0.176

6.119 ± 0.471

4.895 ± 0.323

10.49 ± 0.006

8.042 ± 1.625

6.469 ± 1.852

1.399 ± 0.321

2.797 ± 0.426
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski