Mycobacterium phage PP

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5XVE1|A0A2Z5XVE1_9CAUD Putative scaffolding protein OS=Mycobacterium phage PP OX=2077134 PE=4 SV=1
MM1 pKa = 7.8ASDD4 pKa = 4.56RR5 pKa = 11.84FNLIADD11 pKa = 3.74IAGADD16 pKa = 3.65DD17 pKa = 4.02WYY19 pKa = 10.72DD20 pKa = 3.68LYY22 pKa = 10.9GTALNLHH29 pKa = 6.59FDD31 pKa = 3.24IAEE34 pKa = 4.05VLDD37 pKa = 4.33ASDD40 pKa = 3.89VEE42 pKa = 4.9GDD44 pKa = 3.64VTPGPFARR52 pKa = 11.84WQYY55 pKa = 10.67RR56 pKa = 11.84RR57 pKa = 11.84PPLTVPDD64 pKa = 4.79LEE66 pKa = 4.72TIAARR71 pKa = 11.84AEE73 pKa = 4.09DD74 pKa = 4.12FSEE77 pKa = 5.25GEE79 pKa = 3.85WADD82 pKa = 3.55DD83 pKa = 3.4YY84 pKa = 11.88GYY86 pKa = 9.62ATVALASALLAGEE99 pKa = 4.3ITQADD104 pKa = 4.33LVYY107 pKa = 11.0VGDD110 pKa = 3.73VLSRR114 pKa = 11.84YY115 pKa = 7.73LTLLEE120 pKa = 4.62ANGEE124 pKa = 4.31RR125 pKa = 11.84CCCC128 pKa = 4.12

Molecular weight: 14.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5XVD0|A0A2Z5XVD0_9CAUD Putative transcriptional factor OS=Mycobacterium phage PP OX=2077134 PE=4 SV=1
MM1 pKa = 7.76RR2 pKa = 11.84GYY4 pKa = 8.45LTPHH8 pKa = 5.7NRR10 pKa = 11.84EE11 pKa = 4.0DD12 pKa = 4.71RR13 pKa = 11.84DD14 pKa = 3.54MSKK17 pKa = 8.38QTTVWLDD24 pKa = 2.91GKK26 pKa = 10.58QYY28 pKa = 10.91VIRR31 pKa = 11.84GNNIIRR37 pKa = 11.84VRR39 pKa = 3.51

Molecular weight: 4.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

15719

26

1003

196.5

21.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.441 ± 0.303

0.865 ± 0.114

6.902 ± 0.188

6.922 ± 0.298

3.244 ± 0.17

8.557 ± 0.33

2.239 ± 0.198

4.612 ± 0.157

3.868 ± 0.283

8.009 ± 0.363

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.322 ± 0.115

3.155 ± 0.163

5.439 ± 0.299

3.671 ± 0.261

6.934 ± 0.361

5.407 ± 0.268

5.866 ± 0.274

7.723 ± 0.251

1.953 ± 0.131

2.869 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski