Spirosoma radiotolerans

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Spirosoma

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5328 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3ZVJ5|A0A0E3ZVJ5_9BACT Ribokinase OS=Spirosoma radiotolerans OX=1379870 GN=SD10_15980 PE=4 SV=1
MM1 pKa = 7.67ANYY4 pKa = 10.34KK5 pKa = 10.34NDD7 pKa = 3.59GYY9 pKa = 11.27DD10 pKa = 3.34PEE12 pKa = 5.14EE13 pKa = 4.48ISALKK18 pKa = 10.42DD19 pKa = 3.06EE20 pKa = 5.35CSQEE24 pKa = 4.12GKK26 pKa = 10.51SFIYY30 pKa = 10.96VEE32 pKa = 5.48DD33 pKa = 3.83EE34 pKa = 4.18DD35 pKa = 6.46LDD37 pKa = 3.79VLEE40 pKa = 5.1EE41 pKa = 4.62GEE43 pKa = 4.34CRR45 pKa = 11.84HH46 pKa = 5.86IQFPGQYY53 pKa = 9.18QGQEE57 pKa = 4.23VIFDD61 pKa = 3.85TLVYY65 pKa = 9.37TLRR68 pKa = 11.84LHH70 pKa = 6.7HH71 pKa = 6.86SSLVYY76 pKa = 10.24EE77 pKa = 4.3MAVEE81 pKa = 4.6EE82 pKa = 4.29VQKK85 pKa = 9.56TFPTYY90 pKa = 10.57VPPEE94 pKa = 4.0EE95 pKa = 4.79RR96 pKa = 11.84KK97 pKa = 9.66PNYY100 pKa = 9.91KK101 pKa = 9.68IAPDD105 pKa = 3.77LEE107 pKa = 4.57EE108 pKa = 4.16EE109 pKa = 4.57AEE111 pKa = 4.23TALTEE116 pKa = 4.29IIEE119 pKa = 4.65EE120 pKa = 4.13IEE122 pKa = 3.85EE123 pKa = 4.43TEE125 pKa = 4.21TVKK128 pKa = 10.73VQEE131 pKa = 4.21HH132 pKa = 5.57VEE134 pKa = 3.89VDD136 pKa = 3.54LEE138 pKa = 4.16SDD140 pKa = 3.38YY141 pKa = 11.73GIALDD146 pKa = 3.41VCLNVDD152 pKa = 4.61EE153 pKa = 4.9ITDD156 pKa = 3.65EE157 pKa = 4.32VIEE160 pKa = 4.95DD161 pKa = 4.77FIAHH165 pKa = 6.44FKK167 pKa = 11.4ANTLSLDD174 pKa = 3.32TTLYY178 pKa = 10.74SFSSDD183 pKa = 3.44EE184 pKa = 4.39EE185 pKa = 3.96EE186 pKa = 4.67DD187 pKa = 3.44

Molecular weight: 21.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3ZTF7|A0A0E3ZTF7_9BACT Esterase OS=Spirosoma radiotolerans OX=1379870 GN=SD10_01855 PE=4 SV=1
MM1 pKa = 7.24IRR3 pKa = 11.84HH4 pKa = 5.6VEE6 pKa = 4.09LGATPISRR14 pKa = 11.84LRR16 pKa = 11.84TLSALVGIGRR26 pKa = 11.84VSLGGNRR33 pKa = 11.84PGKK36 pKa = 10.22IYY38 pKa = 11.09GLLTCRR44 pKa = 11.84AGKK47 pKa = 10.04RR48 pKa = 11.84MNVANRR54 pKa = 11.84VFFSDD59 pKa = 3.24EE60 pKa = 3.96AEE62 pKa = 4.7AILAGFRR69 pKa = 11.84PCAVCLPAQYY79 pKa = 10.46KK80 pKa = 9.4AWQRR84 pKa = 11.84EE85 pKa = 3.97QQ86 pKa = 3.17

Molecular weight: 9.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5328

0

5328

1911320

38

4907

358.7

39.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.422 ± 0.032

0.725 ± 0.012

5.355 ± 0.024

5.075 ± 0.031

4.459 ± 0.027

7.207 ± 0.033

1.842 ± 0.015

5.876 ± 0.025

5.098 ± 0.033

10.057 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.15 ± 0.016

4.638 ± 0.029

4.651 ± 0.022

4.472 ± 0.022

5.153 ± 0.025

6.26 ± 0.034

6.55 ± 0.04

6.925 ± 0.031

1.285 ± 0.013

3.799 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski