Wuhan House Fly Virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Sigmavirus; Wuhan sigmavirus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5KXL1|A0A0B5KXL1_9RHAB GDP polyribonucleotidyltransferase OS=Wuhan House Fly Virus 1 OX=1608104 GN=L PE=4 SV=1
MM1 pKa = 7.97DD2 pKa = 4.47VLNLTDD8 pKa = 3.42QEE10 pKa = 4.59TNEE13 pKa = 4.17FLRR16 pKa = 11.84VRR18 pKa = 11.84AIQIEE23 pKa = 4.34KK24 pKa = 8.57TGEE27 pKa = 3.78YY28 pKa = 9.61VYY30 pKa = 10.71PWFEE34 pKa = 3.84QHH36 pKa = 6.44NSKK39 pKa = 10.7PPIKK43 pKa = 9.92IPKK46 pKa = 9.27VDD48 pKa = 3.94DD49 pKa = 3.63AAGARR54 pKa = 11.84LRR56 pKa = 11.84AITKK60 pKa = 8.99GFASGVICSVQTASAFLSWKK80 pKa = 7.7MKK82 pKa = 10.4RR83 pKa = 11.84MTARR87 pKa = 11.84VDD89 pKa = 4.25SVWKK93 pKa = 10.45SYY95 pKa = 10.71NQLIGDD101 pKa = 4.58PNADD105 pKa = 3.24ITPLSLLSVEE115 pKa = 4.7EE116 pKa = 4.29VGTPTISGNEE126 pKa = 4.05VSSSEE131 pKa = 4.93ADD133 pKa = 3.52EE134 pKa = 4.16KK135 pKa = 10.97WIIGYY140 pKa = 8.51ILCLYY145 pKa = 10.56RR146 pKa = 11.84LGRR149 pKa = 11.84VNDD152 pKa = 3.49ANYY155 pKa = 10.53RR156 pKa = 11.84GDD158 pKa = 4.52LIKK161 pKa = 10.87SMKK164 pKa = 9.75PILEE168 pKa = 4.27AQGMPNTTNLLADD181 pKa = 4.24CGKK184 pKa = 7.14TTAWCQCPVFRR195 pKa = 11.84KK196 pKa = 9.86LVAAIDD202 pKa = 3.47MFFYY206 pKa = 10.72RR207 pKa = 11.84FPDD210 pKa = 3.75HH211 pKa = 6.29EE212 pKa = 4.37WSFVRR217 pKa = 11.84IGTISSRR224 pKa = 11.84YY225 pKa = 8.89KK226 pKa = 10.69DD227 pKa = 3.78CTSLLSVAHH236 pKa = 6.06TSRR239 pKa = 11.84LLGFPHH245 pKa = 7.42PAEE248 pKa = 3.82LWTWITNITLHH259 pKa = 7.93HH260 pKa = 6.24EE261 pKa = 4.17MKK263 pKa = 10.92KK264 pKa = 9.28MFNNADD270 pKa = 3.94EE271 pKa = 4.38FHH273 pKa = 7.39DD274 pKa = 4.75PYY276 pKa = 11.4SYY278 pKa = 10.85FPYY281 pKa = 9.78QIEE284 pKa = 4.08MGLSKK289 pKa = 10.66KK290 pKa = 10.25SLYY293 pKa = 8.16STRR296 pKa = 11.84EE297 pKa = 3.58NPGIHH302 pKa = 6.67AFCHH306 pKa = 6.35IIGLYY311 pKa = 7.88YY312 pKa = 10.56QNARR316 pKa = 11.84SMKK319 pKa = 9.24ARR321 pKa = 11.84WMLEE325 pKa = 3.62DD326 pKa = 4.53DD327 pKa = 4.06VNPILFNAHH336 pKa = 5.54LVGFAFTRR344 pKa = 11.84GKK346 pKa = 9.56WADD349 pKa = 3.56KK350 pKa = 8.33FWAVKK355 pKa = 9.78GHH357 pKa = 7.08IGPPAPPARR366 pKa = 11.84QPDD369 pKa = 3.7NAQQEE374 pKa = 4.44PMEE377 pKa = 4.41EE378 pKa = 4.17EE379 pKa = 3.97VDD381 pKa = 3.79EE382 pKa = 5.38EE383 pKa = 5.51DD384 pKa = 4.55IQSLNQDD391 pKa = 3.37GVDD394 pKa = 4.42AIAPPDD400 pKa = 3.77EE401 pKa = 4.57RR402 pKa = 11.84TPQAWTPYY410 pKa = 10.22LDD412 pKa = 3.33AHH414 pKa = 6.28NWTMSPAMVRR424 pKa = 11.84TAKK427 pKa = 10.36LVASGIVVEE436 pKa = 4.67RR437 pKa = 11.84DD438 pKa = 3.08YY439 pKa = 11.54TIAKK443 pKa = 9.82FSKK446 pKa = 9.6TPAFYY451 pKa = 11.28LNMDD455 pKa = 3.99

Molecular weight: 51.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5KTK4|A0A0B5KTK4_9RHAB Glycoprotein OS=Wuhan House Fly Virus 1 OX=1608104 GN=G PE=4 SV=1
MM1 pKa = 7.61SNRR4 pKa = 11.84KK5 pKa = 9.17LSLGSFGKK13 pKa = 10.37AINIWKK19 pKa = 9.46PSAPSITTSSLLDD32 pKa = 3.48GVSSNWKK39 pKa = 10.12AFDD42 pKa = 4.04VFDD45 pKa = 5.89LDD47 pKa = 4.17PEE49 pKa = 4.47PTSSEE54 pKa = 4.41SATHH58 pKa = 6.59PKK60 pKa = 9.92QEE62 pKa = 4.31IVWEE66 pKa = 4.08VDD68 pKa = 2.91AQLTVSTSKK77 pKa = 10.79KK78 pKa = 7.95IAIWEE83 pKa = 4.04DD84 pKa = 3.67LIEE87 pKa = 4.47EE88 pKa = 4.44LDD90 pKa = 4.37AIIDD94 pKa = 3.79HH95 pKa = 6.75YY96 pKa = 11.21YY97 pKa = 10.76GPSNQKK103 pKa = 9.79EE104 pKa = 3.96IFIIIYY110 pKa = 9.64YY111 pKa = 10.81LLGTHH116 pKa = 7.08LKK118 pKa = 10.38PGVGVNGEE126 pKa = 4.06YY127 pKa = 10.43KK128 pKa = 10.54KK129 pKa = 10.89FVIEE133 pKa = 3.59LSKK136 pKa = 10.63RR137 pKa = 11.84IKK139 pKa = 10.25FSYY142 pKa = 10.01NFEE145 pKa = 4.58GIQRR149 pKa = 11.84EE150 pKa = 4.18AGDD153 pKa = 3.69ISWTYY158 pKa = 8.66SQSRR162 pKa = 11.84ARR164 pKa = 11.84KK165 pKa = 8.89RR166 pKa = 11.84LLIDD170 pKa = 3.67FRR172 pKa = 11.84CSITPTKK179 pKa = 10.33RR180 pKa = 11.84DD181 pKa = 3.98GIPLKK186 pKa = 10.5KK187 pKa = 10.54LYY189 pKa = 10.6DD190 pKa = 3.63FQIYY194 pKa = 9.61PNLPHH199 pKa = 6.96PSIGSLLSGNDD210 pKa = 2.88ITVLDD215 pKa = 3.83VGTNYY220 pKa = 10.52SFAKK224 pKa = 9.8KK225 pKa = 7.94QQ226 pKa = 3.2

Molecular weight: 25.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3995

226

2098

665.8

75.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.357 ± 0.697

1.552 ± 0.103

5.682 ± 0.445

5.332 ± 0.122

4.456 ± 0.404

4.881 ± 0.428

2.804 ± 0.272

7.935 ± 0.547

5.932 ± 0.384

10.088 ± 0.969

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.902 ± 0.304

5.257 ± 0.2

4.906 ± 0.538

3.655 ± 0.187

4.781 ± 0.197

8.586 ± 0.447

6.558 ± 0.619

5.081 ± 0.432

1.652 ± 0.298

3.605 ± 0.267
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski