Thermoleophilum album

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Thermoleophilia; Thermoleophilales; Thermoleophilaceae; Thermoleophilum

Average proteome isoelectric point is 7.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2060 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H6FQJ9|A0A1H6FQJ9_THEAL Hemolysin contains CBS domains OS=Thermoleophilum album OX=29539 GN=SAMN02745716_1104 PE=3 SV=1
MM1 pKa = 7.85GDD3 pKa = 3.33AQTTTLQKK11 pKa = 9.95WICTSCGYY19 pKa = 10.23IYY21 pKa = 10.59DD22 pKa = 4.27PAEE25 pKa = 4.29GDD27 pKa = 3.63PDD29 pKa = 4.06GGIPPGTPFEE39 pKa = 5.18EE40 pKa = 5.35IPDD43 pKa = 3.59DD44 pKa = 3.95WFCPVCGARR53 pKa = 11.84KK54 pKa = 9.31RR55 pKa = 11.84DD56 pKa = 3.9FEE58 pKa = 4.4PLEE61 pKa = 4.04EE62 pKa = 4.12

Molecular weight: 6.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H6FJQ5|A0A1H6FJQ5_THEAL Methylthioribose-1-phosphate isomerase OS=Thermoleophilum album OX=29539 GN=mtnA PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.66QPKK8 pKa = 8.52KK9 pKa = 8.29RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 6.24GFLKK20 pKa = 10.42RR21 pKa = 11.84SMTRR25 pKa = 11.84QGRR28 pKa = 11.84AVLKK32 pKa = 9.78RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.35GRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84LAGG44 pKa = 3.4

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2060

0

2060

679472

37

2354

329.8

35.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.012 ± 0.104

1.005 ± 0.014

4.983 ± 0.036

6.952 ± 0.064

2.975 ± 0.03

8.675 ± 0.048

1.886 ± 0.023

3.638 ± 0.04

1.744 ± 0.034

11.276 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.38 ± 0.018

1.601 ± 0.023

5.956 ± 0.04

2.775 ± 0.03

10.575 ± 0.053

4.649 ± 0.032

4.46 ± 0.028

8.337 ± 0.042

1.261 ± 0.024

1.86 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski