Amino acid dipepetide frequency for Rhodococcus phage ReqiPoco6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.725AlaAla: 7.725 ± 0.869
0.286AlaCys: 0.286 ± 0.115
4.741AlaAsp: 4.741 ± 0.415
4.66AlaGlu: 4.66 ± 0.527
3.392AlaPhe: 3.392 ± 0.347
6.948AlaGly: 6.948 ± 0.976
1.635AlaHis: 1.635 ± 0.332
5.436AlaIle: 5.436 ± 0.724
5.027AlaLys: 5.027 ± 0.583
7.725AlaLeu: 7.725 ± 0.769
2.412AlaMet: 2.412 ± 0.396
4.169AlaAsn: 4.169 ± 0.417
2.779AlaPro: 2.779 ± 0.363
2.371AlaGln: 2.371 ± 0.296
3.965AlaArg: 3.965 ± 0.509
6.049AlaSer: 6.049 ± 0.541
5.395AlaThr: 5.395 ± 0.529
6.54AlaVal: 6.54 ± 0.38
1.144AlaTrp: 1.144 ± 0.206
2.902AlaTyr: 2.902 ± 0.322
0.0AlaXaa: 0.0 ± 0.0
Cys
0.327CysAla: 0.327 ± 0.126
0.041CysCys: 0.041 ± 0.041
0.286CysAsp: 0.286 ± 0.102
0.245CysGlu: 0.245 ± 0.106
0.204CysPhe: 0.204 ± 0.09
0.49CysGly: 0.49 ± 0.153
0.041CysHis: 0.041 ± 0.046
0.409CysIle: 0.409 ± 0.145
0.204CysLys: 0.204 ± 0.132
0.94CysLeu: 0.94 ± 0.234
0.123CysMet: 0.123 ± 0.07
0.163CysAsn: 0.163 ± 0.09
0.286CysPro: 0.286 ± 0.124
0.245CysGln: 0.245 ± 0.106
0.531CysArg: 0.531 ± 0.197
0.327CysSer: 0.327 ± 0.117
0.286CysThr: 0.286 ± 0.153
0.286CysVal: 0.286 ± 0.104
0.163CysTrp: 0.163 ± 0.081
0.123CysTyr: 0.123 ± 0.058
0.0CysXaa: 0.0 ± 0.0
Asp
5.436AspAla: 5.436 ± 0.512
0.368AspCys: 0.368 ± 0.14
4.455AspAsp: 4.455 ± 0.469
4.66AspGlu: 4.66 ± 0.476
2.984AspPhe: 2.984 ± 0.413
4.823AspGly: 4.823 ± 0.532
1.022AspHis: 1.022 ± 0.204
4.373AspIle: 4.373 ± 0.533
3.229AspLys: 3.229 ± 0.35
5.027AspLeu: 5.027 ± 0.598
1.676AspMet: 1.676 ± 0.272
2.248AspAsn: 2.248 ± 0.363
2.534AspPro: 2.534 ± 0.364
1.512AspGln: 1.512 ± 0.239
3.229AspArg: 3.229 ± 0.439
4.21AspSer: 4.21 ± 0.441
4.046AspThr: 4.046 ± 0.346
3.679AspVal: 3.679 ± 0.4
0.858AspTrp: 0.858 ± 0.17
1.921AspTyr: 1.921 ± 0.289
0.0AspXaa: 0.0 ± 0.0
Glu
5.232GluAla: 5.232 ± 0.523
0.204GluCys: 0.204 ± 0.095
3.801GluAsp: 3.801 ± 0.437
4.987GluGlu: 4.987 ± 0.726
2.779GluPhe: 2.779 ± 0.347
4.251GluGly: 4.251 ± 0.492
1.104GluHis: 1.104 ± 0.232
4.169GluIle: 4.169 ± 0.345
3.76GluLys: 3.76 ± 0.598
5.64GluLeu: 5.64 ± 0.542
2.166GluMet: 2.166 ± 0.292
2.003GluAsn: 2.003 ± 0.285
2.044GluPro: 2.044 ± 0.328
2.82GluGln: 2.82 ± 0.409
3.924GluArg: 3.924 ± 0.498
2.861GluSer: 2.861 ± 0.356
3.474GluThr: 3.474 ± 0.323
4.169GluVal: 4.169 ± 0.453
1.063GluTrp: 1.063 ± 0.213
2.085GluTyr: 2.085 ± 0.292
0.0GluXaa: 0.0 ± 0.0
Phe
2.085PheAla: 2.085 ± 0.236
0.531PheCys: 0.531 ± 0.173
3.392PheAsp: 3.392 ± 0.375
2.248PheGlu: 2.248 ± 0.303
1.308PhePhe: 1.308 ± 0.25
3.27PheGly: 3.27 ± 0.451
0.49PheHis: 0.49 ± 0.168
1.758PheIle: 1.758 ± 0.242
1.512PheLys: 1.512 ± 0.299
3.147PheLeu: 3.147 ± 0.329
0.817PheMet: 0.817 ± 0.221
2.575PheAsn: 2.575 ± 0.38
1.512PhePro: 1.512 ± 0.179
0.899PheGln: 0.899 ± 0.21
2.371PheArg: 2.371 ± 0.283
1.962PheSer: 1.962 ± 0.326
2.33PheThr: 2.33 ± 0.281
2.575PheVal: 2.575 ± 0.263
0.695PheTrp: 0.695 ± 0.148
1.308PheTyr: 1.308 ± 0.305
0.0PheXaa: 0.0 ± 0.0
Gly
6.908GlyAla: 6.908 ± 1.05
0.45GlyCys: 0.45 ± 0.158
4.782GlyAsp: 4.782 ± 0.372
4.578GlyGlu: 4.578 ± 0.393
2.33GlyPhe: 2.33 ± 0.37
6.131GlyGly: 6.131 ± 0.636
1.063GlyHis: 1.063 ± 0.265
4.864GlyIle: 4.864 ± 0.814
3.515GlyLys: 3.515 ± 0.286
5.927GlyLeu: 5.927 ± 1.095
2.861GlyMet: 2.861 ± 0.511
3.025GlyAsn: 3.025 ± 0.479
2.207GlyPro: 2.207 ± 0.326
1.798GlyGln: 1.798 ± 0.289
4.292GlyArg: 4.292 ± 0.474
5.64GlySer: 5.64 ± 0.456
5.763GlyThr: 5.763 ± 0.453
6.499GlyVal: 6.499 ± 0.722
0.777GlyTrp: 0.777 ± 0.187
2.534GlyTyr: 2.534 ± 0.357
0.0GlyXaa: 0.0 ± 0.0
His
1.39HisAla: 1.39 ± 0.325
0.123HisCys: 0.123 ± 0.069
0.695HisAsp: 0.695 ± 0.169
1.144HisGlu: 1.144 ± 0.202
0.654HisPhe: 0.654 ± 0.183
1.308HisGly: 1.308 ± 0.273
0.613HisHis: 0.613 ± 0.171
1.676HisIle: 1.676 ± 0.3
1.226HisLys: 1.226 ± 0.253
1.308HisLeu: 1.308 ± 0.236
0.45HisMet: 0.45 ± 0.121
1.226HisAsn: 1.226 ± 0.241
1.063HisPro: 1.063 ± 0.244
0.777HisGln: 0.777 ± 0.207
1.144HisArg: 1.144 ± 0.203
1.267HisSer: 1.267 ± 0.218
0.981HisThr: 0.981 ± 0.218
1.226HisVal: 1.226 ± 0.224
0.286HisTrp: 0.286 ± 0.126
1.104HisTyr: 1.104 ± 0.206
0.0HisXaa: 0.0 ± 0.0
Ile
4.741IleAla: 4.741 ± 0.493
0.49IleCys: 0.49 ± 0.17
4.251IleAsp: 4.251 ± 0.444
4.414IleGlu: 4.414 ± 0.64
2.289IlePhe: 2.289 ± 0.278
5.313IleGly: 5.313 ± 0.797
1.226IleHis: 1.226 ± 0.285
2.698IleIle: 2.698 ± 0.352
3.515IleLys: 3.515 ± 0.557
5.354IleLeu: 5.354 ± 0.555
1.185IleMet: 1.185 ± 0.223
2.698IleAsn: 2.698 ± 0.265
2.412IlePro: 2.412 ± 0.332
1.717IleGln: 1.717 ± 0.316
2.82IleArg: 2.82 ± 0.375
4.087IleSer: 4.087 ± 0.438
3.924IleThr: 3.924 ± 0.419
4.455IleVal: 4.455 ± 0.52
0.572IleTrp: 0.572 ± 0.127
1.839IleTyr: 1.839 ± 0.244
0.0IleXaa: 0.0 ± 0.0
Lys
5.273LysAla: 5.273 ± 0.6
0.163LysCys: 0.163 ± 0.091
3.474LysAsp: 3.474 ± 0.505
3.597LysGlu: 3.597 ± 0.417
2.33LysPhe: 2.33 ± 0.412
3.679LysGly: 3.679 ± 0.497
1.512LysHis: 1.512 ± 0.306
3.638LysIle: 3.638 ± 0.408
4.087LysLys: 4.087 ± 0.557
5.068LysLeu: 5.068 ± 0.469
1.921LysMet: 1.921 ± 0.291
2.902LysAsn: 2.902 ± 0.325
2.371LysPro: 2.371 ± 0.333
2.575LysGln: 2.575 ± 0.361
3.065LysArg: 3.065 ± 0.394
3.719LysSer: 3.719 ± 0.382
4.087LysThr: 4.087 ± 0.424
3.801LysVal: 3.801 ± 0.368
1.144LysTrp: 1.144 ± 0.197
1.88LysTyr: 1.88 ± 0.35
0.0LysXaa: 0.0 ± 0.0
Leu
7.888LeuAla: 7.888 ± 0.766
0.163LeuCys: 0.163 ± 0.08
5.232LeuAsp: 5.232 ± 0.541
4.21LeuGlu: 4.21 ± 0.416
2.984LeuPhe: 2.984 ± 0.469
5.477LeuGly: 5.477 ± 0.695
1.431LeuHis: 1.431 ± 0.319
5.027LeuIle: 5.027 ± 0.483
5.886LeuLys: 5.886 ± 0.525
5.354LeuLeu: 5.354 ± 0.601
2.085LeuMet: 2.085 ± 0.32
4.741LeuAsn: 4.741 ± 0.544
3.188LeuPro: 3.188 ± 0.318
2.452LeuGln: 2.452 ± 0.316
3.27LeuArg: 3.27 ± 0.374
5.068LeuSer: 5.068 ± 0.396
6.254LeuThr: 6.254 ± 0.643
6.417LeuVal: 6.417 ± 0.559
0.695LeuTrp: 0.695 ± 0.169
3.147LeuTyr: 3.147 ± 0.446
0.0LeuXaa: 0.0 ± 0.0
Met
2.698MetAla: 2.698 ± 0.333
0.204MetCys: 0.204 ± 0.101
1.471MetAsp: 1.471 ± 0.207
1.267MetGlu: 1.267 ± 0.299
0.858MetPhe: 0.858 ± 0.205
1.758MetGly: 1.758 ± 0.333
0.613MetHis: 0.613 ± 0.164
1.553MetIle: 1.553 ± 0.253
1.717MetLys: 1.717 ± 0.227
2.493MetLeu: 2.493 ± 0.293
0.45MetMet: 0.45 ± 0.134
1.349MetAsn: 1.349 ± 0.209
1.717MetPro: 1.717 ± 0.258
0.531MetGln: 0.531 ± 0.172
1.308MetArg: 1.308 ± 0.257
2.616MetSer: 2.616 ± 0.32
2.044MetThr: 2.044 ± 0.27
1.798MetVal: 1.798 ± 0.221
0.204MetTrp: 0.204 ± 0.092
0.49MetTyr: 0.49 ± 0.153
0.0MetXaa: 0.0 ± 0.0
Asn
4.537AsnAla: 4.537 ± 0.422
0.327AsnCys: 0.327 ± 0.108
3.311AsnAsp: 3.311 ± 0.455
3.147AsnGlu: 3.147 ± 0.462
1.676AsnPhe: 1.676 ± 0.289
3.924AsnGly: 3.924 ± 0.496
0.817AsnHis: 0.817 ± 0.185
2.657AsnIle: 2.657 ± 0.491
2.493AsnLys: 2.493 ± 0.273
3.352AsnLeu: 3.352 ± 0.305
0.981AsnMet: 0.981 ± 0.195
2.371AsnAsn: 2.371 ± 0.376
3.188AsnPro: 3.188 ± 0.486
1.635AsnGln: 1.635 ± 0.234
3.188AsnArg: 3.188 ± 0.355
3.188AsnSer: 3.188 ± 0.369
2.207AsnThr: 2.207 ± 0.382
3.638AsnVal: 3.638 ± 0.398
0.531AsnTrp: 0.531 ± 0.189
1.185AsnTyr: 1.185 ± 0.193
0.0AsnXaa: 0.0 ± 0.0
Pro
3.392ProAla: 3.392 ± 0.351
0.409ProCys: 0.409 ± 0.162
2.657ProAsp: 2.657 ± 0.339
3.106ProGlu: 3.106 ± 0.438
1.471ProPhe: 1.471 ± 0.207
2.943ProGly: 2.943 ± 0.393
0.613ProHis: 0.613 ± 0.166
2.738ProIle: 2.738 ± 0.405
2.738ProLys: 2.738 ± 0.322
2.371ProLeu: 2.371 ± 0.275
1.144ProMet: 1.144 ± 0.261
2.085ProAsn: 2.085 ± 0.283
1.839ProPro: 1.839 ± 0.366
0.654ProGln: 0.654 ± 0.16
2.248ProArg: 2.248 ± 0.339
3.311ProSer: 3.311 ± 0.372
2.82ProThr: 2.82 ± 0.427
3.27ProVal: 3.27 ± 0.331
0.736ProTrp: 0.736 ± 0.154
1.349ProTyr: 1.349 ± 0.213
0.0ProXaa: 0.0 ± 0.0
Gln
3.352GlnAla: 3.352 ± 0.547
0.123GlnCys: 0.123 ± 0.065
1.267GlnAsp: 1.267 ± 0.27
1.839GlnGlu: 1.839 ± 0.236
1.144GlnPhe: 1.144 ± 0.265
1.717GlnGly: 1.717 ± 0.321
0.858GlnHis: 0.858 ± 0.188
1.676GlnIle: 1.676 ± 0.296
2.125GlnLys: 2.125 ± 0.358
3.311GlnLeu: 3.311 ± 0.413
0.858GlnMet: 0.858 ± 0.194
1.471GlnAsn: 1.471 ± 0.212
1.308GlnPro: 1.308 ± 0.215
1.267GlnGln: 1.267 ± 0.204
1.635GlnArg: 1.635 ± 0.243
1.676GlnSer: 1.676 ± 0.274
1.635GlnThr: 1.635 ± 0.282
1.921GlnVal: 1.921 ± 0.349
0.695GlnTrp: 0.695 ± 0.158
1.226GlnTyr: 1.226 ± 0.261
0.0GlnXaa: 0.0 ± 0.0
Arg
3.474ArgAla: 3.474 ± 0.399
0.531ArgCys: 0.531 ± 0.154
2.82ArgAsp: 2.82 ± 0.325
3.719ArgGlu: 3.719 ± 0.453
1.798ArgPhe: 1.798 ± 0.199
2.943ArgGly: 2.943 ± 0.315
1.308ArgHis: 1.308 ± 0.234
3.27ArgIle: 3.27 ± 0.39
3.352ArgLys: 3.352 ± 0.586
3.679ArgLeu: 3.679 ± 0.398
1.431ArgMet: 1.431 ± 0.227
2.534ArgAsn: 2.534 ± 0.364
2.657ArgPro: 2.657 ± 0.355
2.085ArgGln: 2.085 ± 0.289
3.76ArgArg: 3.76 ± 0.608
3.515ArgSer: 3.515 ± 0.343
3.147ArgThr: 3.147 ± 0.389
3.352ArgVal: 3.352 ± 0.419
0.613ArgTrp: 0.613 ± 0.177
2.125ArgTyr: 2.125 ± 0.292
0.0ArgXaa: 0.0 ± 0.0
Ser
5.804SerAla: 5.804 ± 0.512
0.368SerCys: 0.368 ± 0.115
4.414SerAsp: 4.414 ± 0.551
4.128SerGlu: 4.128 ± 0.39
2.003SerPhe: 2.003 ± 0.302
6.294SerGly: 6.294 ± 0.584
1.431SerHis: 1.431 ± 0.245
3.842SerIle: 3.842 ± 0.432
3.76SerLys: 3.76 ± 0.417
5.15SerLeu: 5.15 ± 0.434
2.085SerMet: 2.085 ± 0.324
2.984SerAsn: 2.984 ± 0.378
2.412SerPro: 2.412 ± 0.295
2.289SerGln: 2.289 ± 0.248
2.698SerArg: 2.698 ± 0.356
4.537SerSer: 4.537 ± 0.536
4.782SerThr: 4.782 ± 0.51
4.455SerVal: 4.455 ± 0.469
0.817SerTrp: 0.817 ± 0.191
1.88SerTyr: 1.88 ± 0.288
0.0SerXaa: 0.0 ± 0.0
Thr
5.6ThrAla: 5.6 ± 0.473
0.286ThrCys: 0.286 ± 0.1
3.556ThrAsp: 3.556 ± 0.335
3.556ThrGlu: 3.556 ± 0.275
2.248ThrPhe: 2.248 ± 0.241
4.619ThrGly: 4.619 ± 0.467
1.594ThrHis: 1.594 ± 0.235
3.924ThrIle: 3.924 ± 0.345
4.578ThrLys: 4.578 ± 0.374
5.722ThrLeu: 5.722 ± 0.535
1.431ThrMet: 1.431 ± 0.265
3.188ThrAsn: 3.188 ± 0.423
3.556ThrPro: 3.556 ± 0.401
2.085ThrGln: 2.085 ± 0.319
2.738ThrArg: 2.738 ± 0.341
3.924ThrSer: 3.924 ± 0.425
3.883ThrThr: 3.883 ± 0.558
5.15ThrVal: 5.15 ± 0.498
0.736ThrTrp: 0.736 ± 0.223
2.125ThrTyr: 2.125 ± 0.333
0.0ThrXaa: 0.0 ± 0.0
Val
6.09ValAla: 6.09 ± 0.512
0.327ValCys: 0.327 ± 0.119
4.578ValAsp: 4.578 ± 0.524
3.965ValGlu: 3.965 ± 0.36
2.044ValPhe: 2.044 ± 0.293
6.172ValGly: 6.172 ± 0.714
1.431ValHis: 1.431 ± 0.286
3.883ValIle: 3.883 ± 0.469
5.109ValLys: 5.109 ± 0.41
5.804ValLeu: 5.804 ± 0.468
2.044ValMet: 2.044 ± 0.313
3.719ValAsn: 3.719 ± 0.406
2.779ValPro: 2.779 ± 0.34
2.166ValGln: 2.166 ± 0.281
3.719ValArg: 3.719 ± 0.431
5.273ValSer: 5.273 ± 0.406
4.823ValThr: 4.823 ± 0.415
5.559ValVal: 5.559 ± 0.668
0.899ValTrp: 0.899 ± 0.178
2.371ValTyr: 2.371 ± 0.432
0.0ValXaa: 0.0 ± 0.0
Trp
0.736TrpAla: 0.736 ± 0.166
0.082TrpCys: 0.082 ± 0.057
0.899TrpAsp: 0.899 ± 0.203
0.94TrpGlu: 0.94 ± 0.167
0.409TrpPhe: 0.409 ± 0.12
1.022TrpGly: 1.022 ± 0.246
0.368TrpHis: 0.368 ± 0.137
0.45TrpIle: 0.45 ± 0.131
0.613TrpLys: 0.613 ± 0.155
0.817TrpLeu: 0.817 ± 0.185
0.531TrpMet: 0.531 ± 0.166
0.899TrpAsn: 0.899 ± 0.215
0.45TrpPro: 0.45 ± 0.137
0.409TrpGln: 0.409 ± 0.13
0.695TrpArg: 0.695 ± 0.158
1.308TrpSer: 1.308 ± 0.28
0.572TrpThr: 0.572 ± 0.134
1.308TrpVal: 1.308 ± 0.238
0.163TrpTrp: 0.163 ± 0.104
0.613TrpTyr: 0.613 ± 0.151
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.616TyrAla: 2.616 ± 0.385
0.245TyrCys: 0.245 ± 0.112
2.207TyrAsp: 2.207 ± 0.455
2.125TyrGlu: 2.125 ± 0.395
1.88TyrPhe: 1.88 ± 0.333
2.861TyrGly: 2.861 ± 0.374
0.49TyrHis: 0.49 ± 0.141
1.962TyrIle: 1.962 ± 0.306
1.839TyrLys: 1.839 ± 0.324
2.779TyrLeu: 2.779 ± 0.417
0.45TyrMet: 0.45 ± 0.13
2.003TyrAsn: 2.003 ± 0.282
1.594TyrPro: 1.594 ± 0.252
0.858TyrGln: 0.858 ± 0.183
1.512TyrArg: 1.512 ± 0.336
1.594TyrSer: 1.594 ± 0.246
2.044TyrThr: 2.044 ± 0.369
2.657TyrVal: 2.657 ± 0.288
0.49TyrTrp: 0.49 ± 0.151
1.022TyrTyr: 1.022 ± 0.235
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 107 proteins (24467 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski