Prevotella sp. CAG:617

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; environmental samples

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1928 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7PBZ2|R7PBZ2_9BACT 4HBT domain-containing protein OS=Prevotella sp. CAG:617 OX=1262933 GN=BN736_01539 PE=4 SV=1
MM1 pKa = 7.07MWGTNNFSGDD11 pKa = 3.37GYY13 pKa = 10.95LHH15 pKa = 6.75VEE17 pKa = 4.02PTDD20 pKa = 3.19EE21 pKa = 4.24GFYY24 pKa = 9.91MAWAGDD30 pKa = 3.98GFDD33 pKa = 3.64LTAFGTTEE41 pKa = 4.04EE42 pKa = 4.29EE43 pKa = 3.95AMGALEE49 pKa = 4.97EE50 pKa = 5.53KK51 pKa = 10.19IDD53 pKa = 3.88DD54 pKa = 4.22CHH56 pKa = 7.81RR57 pKa = 11.84MLYY60 pKa = 10.06GAEE63 pKa = 4.1EE64 pKa = 4.05AWW66 pKa = 3.42

Molecular weight: 7.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7PCA7|R7PCA7_9BACT Uncharacterized protein OS=Prevotella sp. CAG:617 OX=1262933 GN=BN736_00431 PE=4 SV=1
MM1 pKa = 7.12FHH3 pKa = 7.19LPEE6 pKa = 5.58ISLLADD12 pKa = 3.68RR13 pKa = 11.84DD14 pKa = 4.03VLALRR19 pKa = 11.84VGIFCQNGIIVPTPTMRR36 pKa = 11.84IVYY39 pKa = 7.03VQPPFVAVFEE49 pKa = 4.24FSEE52 pKa = 5.43GIQAGHH58 pKa = 6.2FVSAGLIARR67 pKa = 11.84LAHH70 pKa = 6.37GGLAAGQQRR79 pKa = 11.84QRR81 pKa = 11.84TFGLGRR87 pKa = 11.84PVGALEE93 pKa = 4.03VDD95 pKa = 3.37IQFVTSHH102 pKa = 6.54APVVGQLEE110 pKa = 4.4RR111 pKa = 11.84GQHH114 pKa = 5.22LKK116 pKa = 10.31ILAKK120 pKa = 9.03QHH122 pKa = 5.82ARR124 pKa = 11.84AACAIQHH131 pKa = 6.35AVRR134 pKa = 11.84PMAQPRR140 pKa = 11.84FVSAKK145 pKa = 10.14GLL147 pKa = 3.61

Molecular weight: 15.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1928

0

1928

649267

29

2341

336.8

37.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.81 ± 0.058

1.446 ± 0.021

5.347 ± 0.039

5.944 ± 0.058

4.328 ± 0.038

6.95 ± 0.051

2.279 ± 0.03

6.189 ± 0.057

5.536 ± 0.057

9.516 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.769 ± 0.028

4.582 ± 0.054

3.995 ± 0.04

4.122 ± 0.038

5.449 ± 0.057

6.024 ± 0.051

5.607 ± 0.048

6.747 ± 0.053

1.231 ± 0.022

4.123 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski