Hubei hepe-like virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KK91|A0A1L3KK91_9VIRU U-box domain-containing protein OS=Hubei hepe-like virus 2 OX=1922895 PE=4 SV=1
MM1 pKa = 7.34TLNLIKK7 pKa = 10.71SHH9 pKa = 6.51FSCAISKK16 pKa = 10.62SLVALPYY23 pKa = 10.35NCPCGHH29 pKa = 7.32LFDD32 pKa = 5.02AVNIFTWVNTNNTCPVSRR50 pKa = 11.84LPLLVQDD57 pKa = 3.92LTFNNTVYY65 pKa = 10.66QFLQEE70 pKa = 4.94LRR72 pKa = 11.84LTDD75 pKa = 3.69DD76 pKa = 4.05TDD78 pKa = 3.56VHH80 pKa = 7.01DD81 pKa = 4.94CATTTATEE89 pKa = 4.11ISRR92 pKa = 11.84GDD94 pKa = 3.38DD95 pKa = 3.75TIAMDD100 pKa = 5.8DD101 pKa = 3.73FTGTNDD107 pKa = 4.54LGVQTDD113 pKa = 3.83EE114 pKa = 4.74FPPLVEE120 pKa = 3.89PFVYY124 pKa = 10.44LGNEE128 pKa = 4.39VITVANLNEE137 pKa = 3.89NEE139 pKa = 3.9RR140 pKa = 11.84RR141 pKa = 11.84YY142 pKa = 10.28LGCGKK147 pKa = 10.2LVDD150 pKa = 4.18LADD153 pKa = 5.0GIFNPISTNPHH164 pKa = 6.05LEE166 pKa = 4.02QVVDD170 pKa = 4.31RR171 pKa = 11.84FNMEE175 pKa = 4.33YY176 pKa = 10.0IRR178 pKa = 11.84TLIPIRR184 pKa = 11.84ADD186 pKa = 3.09HH187 pKa = 6.61PNIVINKK194 pKa = 8.13YY195 pKa = 10.25AYY197 pKa = 7.3QTRR200 pKa = 11.84FDD202 pKa = 4.16LVATAKK208 pKa = 10.27LLCRR212 pKa = 11.84QGYY215 pKa = 8.23FVYY218 pKa = 10.76DD219 pKa = 3.63LFKK222 pKa = 10.79IGMHH226 pKa = 7.3DD227 pKa = 4.5DD228 pKa = 3.41LNQYY232 pKa = 9.22VLYY235 pKa = 8.86STPILVTGQRR245 pKa = 11.84NILSKK250 pKa = 10.67YY251 pKa = 9.85KK252 pKa = 10.68RR253 pKa = 11.84LTNN256 pKa = 3.68

Molecular weight: 29.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KK26|A0A1L3KK26_9VIRU Uncharacterized protein OS=Hubei hepe-like virus 2 OX=1922895 PE=4 SV=1
MM1 pKa = 7.59VYY3 pKa = 10.24RR4 pKa = 11.84GNNRR8 pKa = 11.84NRR10 pKa = 11.84ANNNRR15 pKa = 11.84MMNNKK20 pKa = 9.48QYY22 pKa = 9.64VTVRR26 pKa = 11.84RR27 pKa = 11.84DD28 pKa = 2.93KK29 pKa = 10.94YY30 pKa = 11.17VNLISGKK37 pKa = 9.68KK38 pKa = 8.05QQSQNKK44 pKa = 8.79RR45 pKa = 11.84KK46 pKa = 9.44QPPQQRR52 pKa = 11.84KK53 pKa = 6.56QRR55 pKa = 11.84PIQRR59 pKa = 11.84PIQQVRR65 pKa = 11.84TQVFRR70 pKa = 11.84PTNRR74 pKa = 11.84KK75 pKa = 8.63PLRR78 pKa = 11.84QAARR82 pKa = 11.84KK83 pKa = 7.22TNMMNDD89 pKa = 4.24PYY91 pKa = 11.37AMCRR95 pKa = 11.84LTPFQSRR102 pKa = 11.84GLADD106 pKa = 5.57GIPDD110 pKa = 3.7GSDD113 pKa = 2.98VRR115 pKa = 11.84KK116 pKa = 9.9ILLDD120 pKa = 3.27HH121 pKa = 6.88RR122 pKa = 11.84MSNTFTFGSSGMFNILITPCIPSSIWFNSMNNDD155 pKa = 2.49STFTVNGTTYY165 pKa = 9.88PYY167 pKa = 10.83NITDD171 pKa = 4.36FNWLANTIQQPEE183 pKa = 3.94WRR185 pKa = 11.84GIPVTLLDD193 pKa = 5.03QIGKK197 pKa = 9.56FDD199 pKa = 3.99NAGSIYY205 pKa = 10.68NSGKK209 pKa = 9.8CRR211 pKa = 11.84LVTVGWSLTFIGTTVQNSGQIQVQSIGLTLGDD243 pKa = 5.23NIPNMDD249 pKa = 3.77TFSVYY254 pKa = 10.71NSQSPSNKK262 pKa = 8.67SWNNGQVMIRR272 pKa = 11.84TLNCQPLSDD281 pKa = 3.62GAMSVEE287 pKa = 4.28TKK289 pKa = 10.49VIPLRR294 pKa = 11.84SGAHH298 pKa = 5.19GVLKK302 pKa = 10.59HH303 pKa = 6.21VADD306 pKa = 4.4EE307 pKa = 4.52YY308 pKa = 10.9EE309 pKa = 4.3WTDD312 pKa = 3.28VTPNLCFIASPEE324 pKa = 3.96EE325 pKa = 3.77EE326 pKa = 4.53RR327 pKa = 11.84ICSLKK332 pKa = 10.8HH333 pKa = 5.57NEE335 pKa = 4.23NGTSEE340 pKa = 4.5NVMAHH345 pKa = 6.45WPHH348 pKa = 6.65CAAFDD353 pKa = 4.97DD354 pKa = 4.64DD355 pKa = 3.86WSTTKK360 pKa = 10.87LKK362 pKa = 9.35ITGGTTGASFVLDD375 pKa = 3.72TIYY378 pKa = 10.78CVEE381 pKa = 4.36YY382 pKa = 10.73VPSITSDD389 pKa = 3.01TYY391 pKa = 11.57AIAKK395 pKa = 9.13GSKK398 pKa = 9.1SEE400 pKa = 4.77KK401 pKa = 8.85PTLLKK406 pKa = 10.73NVNDD410 pKa = 4.21AAKK413 pKa = 9.59SLPIGSIGSAIDD425 pKa = 3.53VLKK428 pKa = 10.25TGAAIAAAVFF438 pKa = 3.7

Molecular weight: 48.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1861

256

1167

620.3

70.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.481 ± 0.173

2.311 ± 0.162

6.717 ± 0.558

4.836 ± 1.029

4.997 ± 0.567

5.535 ± 0.38

2.364 ± 0.366

7.254 ± 0.335

7.254 ± 1.407

7.63 ± 0.912

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.633 ± 0.234

7.2 ± 0.68

3.654 ± 0.808

3.815 ± 0.581

4.299 ± 0.48

5.857 ± 0.802

7.577 ± 0.577

5.373 ± 0.761

0.86 ± 0.321

4.352 ± 0.559
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski