Amino acid dipepetide frequency for Bacillus phage phi4J1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.092AlaAla: 6.092 ± 1.473
0.078AlaCys: 0.078 ± 0.075
3.671AlaAsp: 3.671 ± 0.541
5.233AlaGlu: 5.233 ± 0.794
2.109AlaPhe: 2.109 ± 0.388
3.983AlaGly: 3.983 ± 0.595
0.781AlaHis: 0.781 ± 0.249
4.608AlaIle: 4.608 ± 0.634
4.374AlaLys: 4.374 ± 0.623
6.56AlaLeu: 6.56 ± 0.973
1.64AlaMet: 1.64 ± 0.411
2.89AlaAsn: 2.89 ± 0.642
1.562AlaPro: 1.562 ± 0.328
2.343AlaGln: 2.343 ± 0.548
2.734AlaArg: 2.734 ± 0.43
3.436AlaSer: 3.436 ± 0.408
3.827AlaThr: 3.827 ± 0.609
4.452AlaVal: 4.452 ± 0.63
1.328AlaTrp: 1.328 ± 0.283
1.64AlaTyr: 1.64 ± 0.312
0.0AlaXaa: 0.0 ± 0.0
Cys
0.391CysAla: 0.391 ± 0.199
0.234CysCys: 0.234 ± 0.116
0.703CysAsp: 0.703 ± 0.304
0.703CysGlu: 0.703 ± 0.256
0.391CysPhe: 0.391 ± 0.192
0.547CysGly: 0.547 ± 0.206
0.156CysHis: 0.156 ± 0.112
0.156CysIle: 0.156 ± 0.099
0.703CysLys: 0.703 ± 0.264
0.469CysLeu: 0.469 ± 0.208
0.312CysMet: 0.312 ± 0.186
0.469CysAsn: 0.469 ± 0.193
0.156CysPro: 0.156 ± 0.146
0.234CysGln: 0.234 ± 0.145
0.625CysArg: 0.625 ± 0.243
0.469CysSer: 0.469 ± 0.165
0.781CysThr: 0.781 ± 0.23
0.391CysVal: 0.391 ± 0.188
0.156CysTrp: 0.156 ± 0.082
0.625CysTyr: 0.625 ± 0.238
0.0CysXaa: 0.0 ± 0.0
Asp
2.343AspAla: 2.343 ± 0.524
0.234AspCys: 0.234 ± 0.13
2.89AspAsp: 2.89 ± 0.609
4.686AspGlu: 4.686 ± 0.708
1.874AspPhe: 1.874 ± 0.336
5.545AspGly: 5.545 ± 0.686
0.781AspHis: 0.781 ± 0.32
4.217AspIle: 4.217 ± 0.6
4.608AspLys: 4.608 ± 0.569
3.827AspLeu: 3.827 ± 0.589
2.265AspMet: 2.265 ± 0.41
1.874AspAsn: 1.874 ± 0.39
1.953AspPro: 1.953 ± 0.342
1.64AspGln: 1.64 ± 0.371
2.577AspArg: 2.577 ± 0.413
2.812AspSer: 2.812 ± 0.434
3.28AspThr: 3.28 ± 0.469
4.608AspVal: 4.608 ± 0.578
1.093AspTrp: 1.093 ± 0.307
2.265AspTyr: 2.265 ± 0.513
0.0AspXaa: 0.0 ± 0.0
Glu
5.467GluAla: 5.467 ± 0.739
0.703GluCys: 0.703 ± 0.297
3.749GluAsp: 3.749 ± 0.491
6.717GluGlu: 6.717 ± 0.822
3.28GluPhe: 3.28 ± 0.48
4.217GluGly: 4.217 ± 0.559
1.015GluHis: 1.015 ± 0.279
6.951GluIle: 6.951 ± 0.675
7.654GluLys: 7.654 ± 1.015
8.279GluLeu: 8.279 ± 0.908
2.655GluMet: 2.655 ± 0.685
4.296GluAsn: 4.296 ± 0.541
1.796GluPro: 1.796 ± 0.32
4.061GluGln: 4.061 ± 0.556
4.842GluArg: 4.842 ± 0.649
4.139GluSer: 4.139 ± 0.511
4.217GluThr: 4.217 ± 0.575
5.467GluVal: 5.467 ± 0.724
1.562GluTrp: 1.562 ± 0.354
2.734GluTyr: 2.734 ± 0.388
0.0GluXaa: 0.0 ± 0.0
Phe
2.109PheAla: 2.109 ± 0.427
0.547PheCys: 0.547 ± 0.233
2.421PheAsp: 2.421 ± 0.326
2.655PheGlu: 2.655 ± 0.487
1.64PhePhe: 1.64 ± 0.451
3.671PheGly: 3.671 ± 0.573
0.937PheHis: 0.937 ± 0.278
3.749PheIle: 3.749 ± 0.565
4.217PheLys: 4.217 ± 0.465
2.421PheLeu: 2.421 ± 0.379
0.859PheMet: 0.859 ± 0.291
2.187PheAsn: 2.187 ± 0.435
1.093PhePro: 1.093 ± 0.297
1.093PheGln: 1.093 ± 0.3
1.953PheArg: 1.953 ± 0.467
2.109PheSer: 2.109 ± 0.52
1.953PheThr: 1.953 ± 0.431
2.655PheVal: 2.655 ± 0.453
0.625PheTrp: 0.625 ± 0.209
2.031PheTyr: 2.031 ± 0.363
0.0PheXaa: 0.0 ± 0.0
Gly
4.061GlyAla: 4.061 ± 0.958
0.234GlyCys: 0.234 ± 0.134
3.593GlyAsp: 3.593 ± 0.492
4.217GlyGlu: 4.217 ± 0.592
2.655GlyPhe: 2.655 ± 0.574
3.983GlyGly: 3.983 ± 0.524
0.547GlyHis: 0.547 ± 0.205
5.623GlyIle: 5.623 ± 0.777
5.233GlyLys: 5.233 ± 0.725
4.842GlyLeu: 4.842 ± 0.553
1.796GlyMet: 1.796 ± 0.385
3.436GlyAsn: 3.436 ± 0.608
0.937GlyPro: 0.937 ± 0.306
2.265GlyGln: 2.265 ± 0.403
3.28GlyArg: 3.28 ± 0.457
3.124GlySer: 3.124 ± 0.555
4.061GlyThr: 4.061 ± 0.554
4.842GlyVal: 4.842 ± 0.85
1.328GlyTrp: 1.328 ± 0.367
3.358GlyTyr: 3.358 ± 0.53
0.0GlyXaa: 0.0 ± 0.0
His
1.406HisAla: 1.406 ± 0.371
0.156HisCys: 0.156 ± 0.114
0.781HisAsp: 0.781 ± 0.244
1.172HisGlu: 1.172 ± 0.28
0.625HisPhe: 0.625 ± 0.232
0.703HisGly: 0.703 ± 0.212
0.078HisHis: 0.078 ± 0.071
1.484HisIle: 1.484 ± 0.413
1.484HisLys: 1.484 ± 0.384
1.562HisLeu: 1.562 ± 0.399
0.234HisMet: 0.234 ± 0.136
0.703HisAsn: 0.703 ± 0.3
0.391HisPro: 0.391 ± 0.174
0.781HisGln: 0.781 ± 0.273
0.781HisArg: 0.781 ± 0.275
0.703HisSer: 0.703 ± 0.193
0.859HisThr: 0.859 ± 0.266
1.172HisVal: 1.172 ± 0.252
0.234HisTrp: 0.234 ± 0.11
0.469HisTyr: 0.469 ± 0.222
0.0HisXaa: 0.0 ± 0.0
Ile
6.56IleAla: 6.56 ± 0.784
0.937IleCys: 0.937 ± 0.271
5.077IleAsp: 5.077 ± 0.649
6.17IleGlu: 6.17 ± 0.681
3.046IlePhe: 3.046 ± 0.45
3.593IleGly: 3.593 ± 0.44
1.406IleHis: 1.406 ± 0.315
4.686IleIle: 4.686 ± 0.64
6.17IleLys: 6.17 ± 0.733
4.139IleLeu: 4.139 ± 0.637
1.562IleMet: 1.562 ± 0.43
5.311IleAsn: 5.311 ± 0.656
2.577IlePro: 2.577 ± 0.393
2.734IleGln: 2.734 ± 0.504
3.749IleArg: 3.749 ± 0.687
4.608IleSer: 4.608 ± 0.645
3.827IleThr: 3.827 ± 0.535
4.217IleVal: 4.217 ± 0.616
1.015IleTrp: 1.015 ± 0.243
1.874IleTyr: 1.874 ± 0.504
0.0IleXaa: 0.0 ± 0.0
Lys
4.998LysAla: 4.998 ± 0.647
0.391LysCys: 0.391 ± 0.261
5.389LysAsp: 5.389 ± 0.642
10.7LysGlu: 10.7 ± 1.089
2.577LysPhe: 2.577 ± 0.419
4.608LysGly: 4.608 ± 0.578
1.172LysHis: 1.172 ± 0.285
6.248LysIle: 6.248 ± 0.698
8.747LysLys: 8.747 ± 1.195
5.858LysLeu: 5.858 ± 0.604
2.499LysMet: 2.499 ± 0.52
4.998LysAsn: 4.998 ± 0.566
2.734LysPro: 2.734 ± 0.423
4.608LysGln: 4.608 ± 0.803
3.983LysArg: 3.983 ± 0.709
4.139LysSer: 4.139 ± 0.451
4.842LysThr: 4.842 ± 0.733
8.044LysVal: 8.044 ± 0.691
0.703LysTrp: 0.703 ± 0.229
2.968LysTyr: 2.968 ± 0.552
0.0LysXaa: 0.0 ± 0.0
Leu
3.983LeuAla: 3.983 ± 0.623
0.859LeuCys: 0.859 ± 0.288
4.53LeuAsp: 4.53 ± 0.709
7.341LeuGlu: 7.341 ± 0.859
2.89LeuPhe: 2.89 ± 0.491
4.842LeuGly: 4.842 ± 0.788
1.64LeuHis: 1.64 ± 0.366
4.452LeuIle: 4.452 ± 0.615
7.341LeuLys: 7.341 ± 0.931
5.467LeuLeu: 5.467 ± 0.795
2.265LeuMet: 2.265 ± 0.435
5.155LeuAsn: 5.155 ± 0.638
2.187LeuPro: 2.187 ± 0.342
3.436LeuGln: 3.436 ± 0.55
3.515LeuArg: 3.515 ± 0.555
5.467LeuSer: 5.467 ± 0.852
4.139LeuThr: 4.139 ± 0.667
4.842LeuVal: 4.842 ± 0.748
0.703LeuTrp: 0.703 ± 0.244
2.577LeuTyr: 2.577 ± 0.47
0.0LeuXaa: 0.0 ± 0.0
Met
2.031MetAla: 2.031 ± 0.42
0.078MetCys: 0.078 ± 0.094
1.718MetAsp: 1.718 ± 0.457
1.874MetGlu: 1.874 ± 0.444
1.328MetPhe: 1.328 ± 0.426
1.64MetGly: 1.64 ± 0.318
0.391MetHis: 0.391 ± 0.22
1.64MetIle: 1.64 ± 0.36
3.827MetLys: 3.827 ± 0.675
2.265MetLeu: 2.265 ± 0.427
0.547MetMet: 0.547 ± 0.179
2.421MetAsn: 2.421 ± 0.503
1.093MetPro: 1.093 ± 0.33
1.25MetGln: 1.25 ± 0.321
1.64MetArg: 1.64 ± 0.323
1.796MetSer: 1.796 ± 0.332
1.796MetThr: 1.796 ± 0.37
1.172MetVal: 1.172 ± 0.254
0.312MetTrp: 0.312 ± 0.169
0.547MetTyr: 0.547 ± 0.216
0.0MetXaa: 0.0 ± 0.0
Asn
3.671AsnAla: 3.671 ± 0.754
0.625AsnCys: 0.625 ± 0.23
3.124AsnAsp: 3.124 ± 0.566
5.389AsnGlu: 5.389 ± 0.649
1.796AsnPhe: 1.796 ± 0.298
5.077AsnGly: 5.077 ± 0.678
0.859AsnHis: 0.859 ± 0.243
3.593AsnIle: 3.593 ± 0.472
4.998AsnLys: 4.998 ± 0.642
4.998AsnLeu: 4.998 ± 0.521
1.64AsnMet: 1.64 ± 0.41
3.202AsnAsn: 3.202 ± 0.533
2.655AsnPro: 2.655 ± 0.485
1.953AsnGln: 1.953 ± 0.342
1.796AsnArg: 1.796 ± 0.333
2.265AsnSer: 2.265 ± 0.462
3.124AsnThr: 3.124 ± 0.49
2.343AsnVal: 2.343 ± 0.467
0.547AsnTrp: 0.547 ± 0.191
1.562AsnTyr: 1.562 ± 0.382
0.0AsnXaa: 0.0 ± 0.0
Pro
1.874ProAla: 1.874 ± 0.301
0.234ProCys: 0.234 ± 0.161
1.562ProAsp: 1.562 ± 0.383
2.265ProGlu: 2.265 ± 0.617
1.874ProPhe: 1.874 ± 0.364
1.484ProGly: 1.484 ± 0.375
0.547ProHis: 0.547 ± 0.196
2.031ProIle: 2.031 ± 0.481
2.655ProLys: 2.655 ± 0.549
2.655ProLeu: 2.655 ± 0.535
0.937ProMet: 0.937 ± 0.239
1.406ProAsn: 1.406 ± 0.317
1.406ProPro: 1.406 ± 0.33
1.328ProGln: 1.328 ± 0.428
0.312ProArg: 0.312 ± 0.149
1.953ProSer: 1.953 ± 0.374
2.577ProThr: 2.577 ± 0.491
2.187ProVal: 2.187 ± 0.527
0.312ProTrp: 0.312 ± 0.153
1.64ProTyr: 1.64 ± 0.354
0.0ProXaa: 0.0 ± 0.0
Gln
2.734GlnAla: 2.734 ± 0.574
0.156GlnCys: 0.156 ± 0.116
1.484GlnAsp: 1.484 ± 0.378
3.749GlnGlu: 3.749 ± 0.566
2.187GlnPhe: 2.187 ± 0.473
2.499GlnGly: 2.499 ± 0.473
0.859GlnHis: 0.859 ± 0.292
2.812GlnIle: 2.812 ± 0.426
3.593GlnLys: 3.593 ± 0.592
3.749GlnLeu: 3.749 ± 0.694
1.953GlnMet: 1.953 ± 0.469
2.031GlnAsn: 2.031 ± 0.419
1.093GlnPro: 1.093 ± 0.226
2.109GlnGln: 2.109 ± 0.54
1.718GlnArg: 1.718 ± 0.444
2.031GlnSer: 2.031 ± 0.376
2.187GlnThr: 2.187 ± 0.419
1.874GlnVal: 1.874 ± 0.388
0.703GlnTrp: 0.703 ± 0.233
1.64GlnTyr: 1.64 ± 0.363
0.0GlnXaa: 0.0 ± 0.0
Arg
2.577ArgAla: 2.577 ± 0.562
0.937ArgCys: 0.937 ± 0.291
1.874ArgAsp: 1.874 ± 0.329
3.28ArgGlu: 3.28 ± 0.502
2.421ArgPhe: 2.421 ± 0.546
2.89ArgGly: 2.89 ± 0.436
1.015ArgHis: 1.015 ± 0.282
4.374ArgIle: 4.374 ± 0.762
3.905ArgLys: 3.905 ± 0.606
3.515ArgLeu: 3.515 ± 0.476
1.015ArgMet: 1.015 ± 0.264
2.499ArgAsn: 2.499 ± 0.467
1.015ArgPro: 1.015 ± 0.249
1.328ArgGln: 1.328 ± 0.294
2.109ArgArg: 2.109 ± 0.431
2.109ArgSer: 2.109 ± 0.432
1.953ArgThr: 1.953 ± 0.396
3.749ArgVal: 3.749 ± 0.69
0.625ArgTrp: 0.625 ± 0.2
1.796ArgTyr: 1.796 ± 0.387
0.0ArgXaa: 0.0 ± 0.0
Ser
2.577SerAla: 2.577 ± 0.531
0.312SerCys: 0.312 ± 0.169
2.421SerAsp: 2.421 ± 0.524
3.827SerGlu: 3.827 ± 0.478
3.124SerPhe: 3.124 ± 0.465
3.749SerGly: 3.749 ± 0.654
0.859SerHis: 0.859 ± 0.303
4.452SerIle: 4.452 ± 0.637
5.936SerLys: 5.936 ± 0.563
3.827SerLeu: 3.827 ± 0.521
1.874SerMet: 1.874 ± 0.354
3.515SerAsn: 3.515 ± 0.513
1.64SerPro: 1.64 ± 0.324
2.499SerGln: 2.499 ± 0.415
2.499SerArg: 2.499 ± 0.423
2.655SerSer: 2.655 ± 0.58
2.421SerThr: 2.421 ± 0.479
3.436SerVal: 3.436 ± 0.609
0.625SerTrp: 0.625 ± 0.228
1.562SerTyr: 1.562 ± 0.34
0.0SerXaa: 0.0 ± 0.0
Thr
3.905ThrAla: 3.905 ± 0.62
0.156ThrCys: 0.156 ± 0.117
2.655ThrAsp: 2.655 ± 0.648
3.983ThrGlu: 3.983 ± 0.592
3.202ThrPhe: 3.202 ± 0.5
3.358ThrGly: 3.358 ± 0.523
1.015ThrHis: 1.015 ± 0.288
4.53ThrIle: 4.53 ± 0.574
4.92ThrLys: 4.92 ± 0.736
4.53ThrLeu: 4.53 ± 0.618
1.874ThrMet: 1.874 ± 0.408
2.968ThrAsn: 2.968 ± 0.456
3.046ThrPro: 3.046 ± 0.496
2.031ThrGln: 2.031 ± 0.353
1.484ThrArg: 1.484 ± 0.36
1.953ThrSer: 1.953 ± 0.464
3.28ThrThr: 3.28 ± 0.643
3.749ThrVal: 3.749 ± 0.65
0.703ThrTrp: 0.703 ± 0.223
2.265ThrTyr: 2.265 ± 0.43
0.0ThrXaa: 0.0 ± 0.0
Val
4.139ValAla: 4.139 ± 0.457
0.859ValCys: 0.859 ± 0.226
4.217ValAsp: 4.217 ± 0.681
5.936ValGlu: 5.936 ± 0.834
1.874ValPhe: 1.874 ± 0.475
4.061ValGly: 4.061 ± 0.575
0.859ValHis: 0.859 ± 0.268
4.92ValIle: 4.92 ± 0.618
5.858ValLys: 5.858 ± 0.749
4.842ValLeu: 4.842 ± 0.555
1.718ValMet: 1.718 ± 0.421
3.515ValAsn: 3.515 ± 0.615
2.343ValPro: 2.343 ± 0.361
3.202ValGln: 3.202 ± 0.509
2.499ValArg: 2.499 ± 0.565
4.686ValSer: 4.686 ± 0.607
4.217ValThr: 4.217 ± 0.924
3.749ValVal: 3.749 ± 0.547
0.781ValTrp: 0.781 ± 0.235
1.874ValTyr: 1.874 ± 0.474
0.0ValXaa: 0.0 ± 0.0
Trp
0.547TrpAla: 0.547 ± 0.204
0.312TrpCys: 0.312 ± 0.183
1.484TrpAsp: 1.484 ± 0.341
1.406TrpGlu: 1.406 ± 0.25
0.391TrpPhe: 0.391 ± 0.175
1.093TrpGly: 1.093 ± 0.293
0.156TrpHis: 0.156 ± 0.106
0.703TrpIle: 0.703 ± 0.257
1.093TrpLys: 1.093 ± 0.268
1.25TrpLeu: 1.25 ± 0.288
0.312TrpMet: 0.312 ± 0.168
0.937TrpAsn: 0.937 ± 0.257
0.156TrpPro: 0.156 ± 0.138
0.547TrpGln: 0.547 ± 0.238
0.625TrpArg: 0.625 ± 0.195
0.859TrpSer: 0.859 ± 0.29
0.781TrpThr: 0.781 ± 0.225
0.859TrpVal: 0.859 ± 0.256
0.156TrpTrp: 0.156 ± 0.091
0.547TrpTyr: 0.547 ± 0.213
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.874TyrAla: 1.874 ± 0.349
0.625TyrCys: 0.625 ± 0.238
2.031TyrAsp: 2.031 ± 0.425
2.499TyrGlu: 2.499 ± 0.353
1.64TyrPhe: 1.64 ± 0.327
1.874TyrGly: 1.874 ± 0.309
0.703TyrHis: 0.703 ± 0.303
2.031TyrIle: 2.031 ± 0.392
2.89TyrLys: 2.89 ± 0.605
2.577TyrLeu: 2.577 ± 0.437
1.328TyrMet: 1.328 ± 0.408
1.484TyrAsn: 1.484 ± 0.4
1.406TyrPro: 1.406 ± 0.371
1.718TyrGln: 1.718 ± 0.425
2.265TyrArg: 2.265 ± 0.505
2.499TyrSer: 2.499 ± 0.449
1.484TyrThr: 1.484 ± 0.305
2.343TyrVal: 2.343 ± 0.479
0.703TyrTrp: 0.703 ± 0.234
1.874TyrTyr: 1.874 ± 0.454
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (12805 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski