Bifidobacterium eulemuris

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2326 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A261GDI2|A0A261GDI2_9BIFI Uncharacterized protein OS=Bifidobacterium eulemuris OX=1765219 GN=BEUL_0719 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.12RR3 pKa = 11.84GKK5 pKa = 8.58LTAVVAFTVSAMLALSGCGGSDD27 pKa = 3.16TATDD31 pKa = 3.41AVDD34 pKa = 3.35NGEE37 pKa = 4.65EE38 pKa = 4.03ITLDD42 pKa = 3.7VYY44 pKa = 11.81DD45 pKa = 5.59DD46 pKa = 3.7FANYY50 pKa = 10.25AGEE53 pKa = 4.15QKK55 pKa = 10.7GWFAQVIKK63 pKa = 10.94DD64 pKa = 3.64KK65 pKa = 11.38FNIKK69 pKa = 10.49LNIIAPNVSGGGSTLFDD86 pKa = 3.35TRR88 pKa = 11.84SAAGDD93 pKa = 3.52LGDD96 pKa = 3.65IVLYY100 pKa = 10.25NANNHH105 pKa = 4.97STADD109 pKa = 4.18LIKK112 pKa = 10.65AGLIADD118 pKa = 4.06MTPYY122 pKa = 10.82LDD124 pKa = 3.67DD125 pKa = 4.34AEE127 pKa = 4.7YY128 pKa = 10.4IKK130 pKa = 10.56QYY132 pKa = 11.36SSATDD137 pKa = 3.59SINEE141 pKa = 3.95VAGQEE146 pKa = 4.15SGVWGIPGSVSAQSPEE162 pKa = 3.94TSSEE166 pKa = 3.97GNEE169 pKa = 4.08PTFGPYY175 pKa = 9.36IRR177 pKa = 11.84WDD179 pKa = 3.51YY180 pKa = 9.88YY181 pKa = 11.74AEE183 pKa = 3.98IGYY186 pKa = 9.04PEE188 pKa = 4.75IKK190 pKa = 10.88DD191 pKa = 3.6MDD193 pKa = 4.53DD194 pKa = 3.85LLDD197 pKa = 3.71VLEE200 pKa = 4.33QMQEE204 pKa = 3.68QARR207 pKa = 11.84ADD209 pKa = 3.63TGSTDD214 pKa = 3.27VYY216 pKa = 11.22ALSLFKK222 pKa = 10.93DD223 pKa = 3.2WDD225 pKa = 4.07DD226 pKa = 4.93NMMNNAKK233 pKa = 9.96QPTCFYY239 pKa = 10.68GFDD242 pKa = 3.32EE243 pKa = 4.63MGFVLAKK250 pKa = 10.47ADD252 pKa = 3.53GSDD255 pKa = 3.71YY256 pKa = 11.3EE257 pKa = 4.6DD258 pKa = 3.55VSEE261 pKa = 4.38EE262 pKa = 3.98GGIYY266 pKa = 10.11EE267 pKa = 3.98QTLEE271 pKa = 4.56FFNQAYY277 pKa = 9.53QRR279 pKa = 11.84GLVDD283 pKa = 4.55PEE285 pKa = 5.06SSTQSYY291 pKa = 8.29DD292 pKa = 2.94TMYY295 pKa = 11.42AKK297 pKa = 10.49YY298 pKa = 10.17QEE300 pKa = 4.49GNVLFSFWPWLGQAAYY316 pKa = 7.96NTTEE320 pKa = 3.81NKK322 pKa = 10.28AAGKK326 pKa = 10.63GFMLAPLEE334 pKa = 4.24DD335 pKa = 3.81MQIFSYY341 pKa = 10.71GATPNGGTYY350 pKa = 10.5VIALGAKK357 pKa = 9.46AEE359 pKa = 4.06YY360 pKa = 9.76KK361 pKa = 9.8QRR363 pKa = 11.84CVDD366 pKa = 4.37FINWLYY372 pKa = 10.9SPEE375 pKa = 4.43GIYY378 pKa = 10.72ASGGQTSGSSAGVEE392 pKa = 4.01GLTWEE397 pKa = 4.78LNDD400 pKa = 4.84DD401 pKa = 4.32GEE403 pKa = 4.64PVLTDD408 pKa = 3.27FGYY411 pKa = 9.62QVKK414 pKa = 9.78YY415 pKa = 10.91GSGGTVPDD423 pKa = 3.99EE424 pKa = 4.27YY425 pKa = 11.53GGGNYY430 pKa = 9.16TDD432 pKa = 4.57GVSALNFVTVLASDD446 pKa = 3.97IDD448 pKa = 4.14PEE450 pKa = 4.02TGYY453 pKa = 11.29AYY455 pKa = 10.06DD456 pKa = 3.6ASLWPSVIEE465 pKa = 4.38SQFSTALDD473 pKa = 3.95NDD475 pKa = 3.77WSEE478 pKa = 4.31HH479 pKa = 4.91MGGAKK484 pKa = 8.84TAMEE488 pKa = 4.04YY489 pKa = 11.16LEE491 pKa = 5.54ANDD494 pKa = 5.17KK495 pKa = 11.01ILVAPGASFVKK506 pKa = 10.62PEE508 pKa = 4.86DD509 pKa = 4.0DD510 pKa = 3.85SQISTLRR517 pKa = 11.84GQLKK521 pKa = 6.95TTVVNASWQAVFASSDD537 pKa = 3.79SEE539 pKa = 4.21FQSILDD545 pKa = 3.64GMRR548 pKa = 11.84TDD550 pKa = 5.38LEE552 pKa = 4.28GLGYY556 pKa = 10.58DD557 pKa = 3.4QVLEE561 pKa = 4.03VDD563 pKa = 4.0MKK565 pKa = 10.95NAQDD569 pKa = 3.02QDD571 pKa = 3.49AARR574 pKa = 11.84KK575 pKa = 9.67QIVEE579 pKa = 4.75DD580 pKa = 3.9YY581 pKa = 10.81QSEE584 pKa = 4.26NN585 pKa = 3.14

Molecular weight: 63.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A261G1F4|A0A261G1F4_9BIFI Sodium:proton antiporter OS=Bifidobacterium eulemuris OX=1765219 GN=BEUL_2004 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.82MKK15 pKa = 9.64HH16 pKa = 5.7GFRR19 pKa = 11.84LRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84SGRR28 pKa = 11.84AVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.06GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2326

0

2326

844624

29

3136

363.1

39.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.154 ± 0.065

0.98 ± 0.018

6.76 ± 0.048

5.961 ± 0.046

3.488 ± 0.034

8.081 ± 0.04

1.99 ± 0.029

5.096 ± 0.043

3.288 ± 0.04

8.863 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.669 ± 0.025

3.202 ± 0.034

4.474 ± 0.034

3.263 ± 0.028

6.146 ± 0.056

6.15 ± 0.048

6.143 ± 0.045

7.963 ± 0.047

1.458 ± 0.021

2.866 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski