Clostridium phage CPD1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5H2QTW5|A0A5H2QTW5_9CAUD Uncharacterized protein OS=Clostridium phage CPD1 OX=2420238 GN=CPD1_036 PE=4 SV=1
MM1 pKa = 7.96DD2 pKa = 5.4KK3 pKa = 10.95DD4 pKa = 4.65KK5 pKa = 11.1EE6 pKa = 4.19IQLLSNEE13 pKa = 4.04TFMEE17 pKa = 4.06IMKK20 pKa = 9.54HH21 pKa = 5.0QRR23 pKa = 11.84QTLEE27 pKa = 4.24GYY29 pKa = 10.44KK30 pKa = 10.0KK31 pKa = 10.09ICLACLATVVLSITIMCGSAIYY53 pKa = 9.83FFANYY58 pKa = 8.15EE59 pKa = 3.91AEE61 pKa = 4.58IIEE64 pKa = 4.58EE65 pKa = 4.26TTTTYY70 pKa = 10.23EE71 pKa = 3.91QQSDD75 pKa = 4.33GEE77 pKa = 4.35SQIINGNSYY86 pKa = 11.18NDD88 pKa = 3.66NSIHH92 pKa = 6.26NAKK95 pKa = 10.1EE96 pKa = 3.81DD97 pKa = 3.35

Molecular weight: 11.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5H2QU56|A0A5H2QU56_9CAUD Uncharacterized protein OS=Clostridium phage CPD1 OX=2420238 GN=CPD1_004 PE=4 SV=1
MM1 pKa = 7.1KK2 pKa = 9.82WNYY5 pKa = 10.41RR6 pKa = 11.84KK7 pKa = 9.58FLRR10 pKa = 11.84NMFFTVVAPILVVAIAFIGALIADD34 pKa = 4.54WFYY37 pKa = 11.76NLPNWFGFF45 pKa = 3.78

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

11913

39

940

180.5

20.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.07 ± 0.482

1.259 ± 0.172

5.431 ± 0.249

8.621 ± 0.499

4.214 ± 0.19

5.918 ± 0.42

1.041 ± 0.143

8.629 ± 0.344

9.712 ± 0.519

8.26 ± 0.313

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.644 ± 0.207

7.681 ± 0.521

2.35 ± 0.161

3.458 ± 0.198

3.878 ± 0.241

5.7 ± 0.335

5.549 ± 0.254

5.305 ± 0.247

1.024 ± 0.107

4.256 ± 0.235
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski