Amino acid dipepetide frequency for Variovorax sp. MHTC-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.609AlaAla: 19.609 ± 0.152
1.357AlaCys: 1.357 ± 0.031
6.503AlaAsp: 6.503 ± 0.075
7.203AlaGlu: 7.203 ± 0.071
4.379AlaPhe: 4.379 ± 0.061
11.689AlaGly: 11.689 ± 0.116
2.702AlaHis: 2.702 ± 0.041
5.453AlaIle: 5.453 ± 0.065
4.04AlaLys: 4.04 ± 0.07
15.375AlaLeu: 15.375 ± 0.14
3.561AlaMet: 3.561 ± 0.045
2.954AlaAsn: 2.954 ± 0.055
6.65AlaPro: 6.65 ± 0.076
5.648AlaGln: 5.648 ± 0.074
9.58AlaArg: 9.58 ± 0.09
7.16AlaSer: 7.16 ± 0.079
6.209AlaThr: 6.209 ± 0.073
9.185AlaVal: 9.185 ± 0.072
1.964AlaTrp: 1.964 ± 0.04
2.466AlaTyr: 2.466 ± 0.036
0.0AlaXaa: 0.0 ± 0.0
Cys
1.219CysAla: 1.219 ± 0.03
0.13CysCys: 0.13 ± 0.008
0.528CysAsp: 0.528 ± 0.019
0.569CysGlu: 0.569 ± 0.02
0.312CysPhe: 0.312 ± 0.014
0.978CysGly: 0.978 ± 0.029
0.236CysHis: 0.236 ± 0.012
0.427CysIle: 0.427 ± 0.016
0.248CysLys: 0.248 ± 0.013
0.761CysLeu: 0.761 ± 0.022
0.21CysMet: 0.21 ± 0.01
0.239CysAsn: 0.239 ± 0.013
0.461CysPro: 0.461 ± 0.017
0.215CysGln: 0.215 ± 0.012
0.612CysArg: 0.612 ± 0.02
0.495CysSer: 0.495 ± 0.018
0.497CysThr: 0.497 ± 0.017
0.711CysVal: 0.711 ± 0.021
0.143CysTrp: 0.143 ± 0.01
0.192CysTyr: 0.192 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
7.576AspAla: 7.576 ± 0.082
0.471AspCys: 0.471 ± 0.017
2.618AspAsp: 2.618 ± 0.044
3.233AspGlu: 3.233 ± 0.045
2.017AspPhe: 2.017 ± 0.037
4.514AspGly: 4.514 ± 0.068
1.052AspHis: 1.052 ± 0.026
2.327AspIle: 2.327 ± 0.039
1.582AspLys: 1.582 ± 0.03
5.142AspLeu: 5.142 ± 0.05
1.102AspMet: 1.102 ± 0.026
1.168AspAsn: 1.168 ± 0.031
2.953AspPro: 2.953 ± 0.043
1.404AspGln: 1.404 ± 0.031
3.437AspArg: 3.437 ± 0.049
2.272AspSer: 2.272 ± 0.036
2.433AspThr: 2.433 ± 0.043
3.797AspVal: 3.797 ± 0.046
0.862AspTrp: 0.862 ± 0.022
1.331AspTyr: 1.331 ± 0.028
0.0AspXaa: 0.0 ± 0.0
Glu
7.394GluAla: 7.394 ± 0.071
0.408GluCys: 0.408 ± 0.016
2.259GluAsp: 2.259 ± 0.043
2.726GluGlu: 2.726 ± 0.049
1.818GluPhe: 1.818 ± 0.034
4.035GluGly: 4.035 ± 0.053
1.31GluHis: 1.31 ± 0.027
2.748GluIle: 2.748 ± 0.043
1.797GluLys: 1.797 ± 0.033
6.239GluLeu: 6.239 ± 0.067
1.303GluMet: 1.303 ± 0.027
1.201GluAsn: 1.201 ± 0.028
2.68GluPro: 2.68 ± 0.041
2.307GluGln: 2.307 ± 0.049
5.139GluArg: 5.139 ± 0.068
2.651GluSer: 2.651 ± 0.041
2.487GluThr: 2.487 ± 0.043
4.111GluVal: 4.111 ± 0.048
0.737GluTrp: 0.737 ± 0.02
0.993GluTyr: 0.993 ± 0.026
0.0GluXaa: 0.0 ± 0.0
Phe
4.531PheAla: 4.531 ± 0.058
0.409PheCys: 0.409 ± 0.017
2.6PheAsp: 2.6 ± 0.036
2.209PheGlu: 2.209 ± 0.039
1.32PhePhe: 1.32 ± 0.035
3.54PheGly: 3.54 ± 0.048
0.751PheHis: 0.751 ± 0.021
1.518PheIle: 1.518 ± 0.029
1.171PheLys: 1.171 ± 0.027
2.944PheLeu: 2.944 ± 0.043
0.781PheMet: 0.781 ± 0.025
1.063PheAsn: 1.063 ± 0.023
1.49PhePro: 1.49 ± 0.03
0.988PheGln: 0.988 ± 0.025
1.982PheArg: 1.982 ± 0.041
2.13PheSer: 2.13 ± 0.036
1.84PheThr: 1.84 ± 0.034
2.911PheVal: 2.911 ± 0.041
0.505PheTrp: 0.505 ± 0.018
0.783PheTyr: 0.783 ± 0.022
0.0PheXaa: 0.0 ± 0.0
Gly
10.117GlyAla: 10.117 ± 0.109
0.906GlyCys: 0.906 ± 0.022
3.974GlyAsp: 3.974 ± 0.054
4.632GlyGlu: 4.632 ± 0.054
3.345GlyPhe: 3.345 ± 0.047
7.366GlyGly: 7.366 ± 0.117
1.875GlyHis: 1.875 ± 0.038
4.117GlyIle: 4.117 ± 0.047
3.172GlyLys: 3.172 ± 0.052
8.785GlyLeu: 8.785 ± 0.085
2.247GlyMet: 2.247 ± 0.032
2.174GlyAsn: 2.174 ± 0.047
3.269GlyPro: 3.269 ± 0.045
3.01GlyGln: 3.01 ± 0.041
5.862GlyArg: 5.862 ± 0.068
4.948GlySer: 4.948 ± 0.078
4.865GlyThr: 4.865 ± 0.118
6.495GlyVal: 6.495 ± 0.058
1.481GlyTrp: 1.481 ± 0.032
2.294GlyTyr: 2.294 ± 0.038
0.0GlyXaa: 0.0 ± 0.0
His
3.029HisAla: 3.029 ± 0.046
0.265HisCys: 0.265 ± 0.012
1.116HisAsp: 1.116 ± 0.027
1.198HisGlu: 1.198 ± 0.028
0.852HisPhe: 0.852 ± 0.021
2.167HisGly: 2.167 ± 0.037
0.622HisHis: 0.622 ± 0.02
0.867HisIle: 0.867 ± 0.024
0.559HisLys: 0.559 ± 0.019
2.162HisLeu: 2.162 ± 0.04
0.481HisMet: 0.481 ± 0.015
0.501HisAsn: 0.501 ± 0.015
1.466HisPro: 1.466 ± 0.034
0.666HisGln: 0.666 ± 0.016
1.581HisArg: 1.581 ± 0.028
1.03HisSer: 1.03 ± 0.025
0.971HisThr: 0.971 ± 0.023
1.633HisVal: 1.633 ± 0.03
0.387HisTrp: 0.387 ± 0.016
0.57HisTyr: 0.57 ± 0.016
0.0HisXaa: 0.0 ± 0.0
Ile
6.734IleAla: 6.734 ± 0.063
0.438IleCys: 0.438 ± 0.016
3.24IleAsp: 3.24 ± 0.044
3.327IleGlu: 3.327 ± 0.049
1.224IlePhe: 1.224 ± 0.03
4.475IleGly: 4.475 ± 0.054
0.861IleHis: 0.861 ± 0.021
1.259IleIle: 1.259 ± 0.032
1.39IleLys: 1.39 ± 0.037
3.048IleLeu: 3.048 ± 0.049
0.67IleMet: 0.67 ± 0.02
1.298IleAsn: 1.298 ± 0.035
2.01IlePro: 2.01 ± 0.037
1.191IleGln: 1.191 ± 0.024
2.582IleArg: 2.582 ± 0.034
2.314IleSer: 2.314 ± 0.035
2.291IleThr: 2.291 ± 0.041
3.712IleVal: 3.712 ± 0.05
0.469IleTrp: 0.469 ± 0.017
0.918IleTyr: 0.918 ± 0.024
0.0IleXaa: 0.0 ± 0.0
Lys
3.909LysAla: 3.909 ± 0.06
0.147LysCys: 0.147 ± 0.01
1.566LysAsp: 1.566 ± 0.032
1.544LysGlu: 1.544 ± 0.035
0.935LysPhe: 0.935 ± 0.027
2.37LysGly: 2.37 ± 0.045
0.635LysHis: 0.635 ± 0.019
1.418LysIle: 1.418 ± 0.036
1.404LysLys: 1.404 ± 0.041
3.637LysLeu: 3.637 ± 0.056
0.714LysMet: 0.714 ± 0.02
0.901LysAsn: 0.901 ± 0.024
2.067LysPro: 2.067 ± 0.044
1.093LysGln: 1.093 ± 0.028
2.214LysArg: 2.214 ± 0.04
1.807LysSer: 1.807 ± 0.038
1.896LysThr: 1.896 ± 0.032
2.436LysVal: 2.436 ± 0.043
0.362LysTrp: 0.362 ± 0.014
0.65LysTyr: 0.65 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
15.493LeuAla: 15.493 ± 0.122
1.006LeuCys: 1.006 ± 0.027
5.772LeuAsp: 5.772 ± 0.061
5.161LeuGlu: 5.161 ± 0.067
3.476LeuPhe: 3.476 ± 0.054
8.713LeuGly: 8.713 ± 0.08
2.414LeuHis: 2.414 ± 0.038
4.282LeuIle: 4.282 ± 0.07
3.343LeuLys: 3.343 ± 0.049
11.059LeuLeu: 11.059 ± 0.126
2.47LeuMet: 2.47 ± 0.041
2.536LeuAsn: 2.536 ± 0.04
6.12LeuPro: 6.12 ± 0.061
3.948LeuGln: 3.948 ± 0.047
7.87LeuArg: 7.87 ± 0.075
6.013LeuSer: 6.013 ± 0.063
5.006LeuThr: 5.006 ± 0.056
7.9LeuVal: 7.9 ± 0.081
1.323LeuTrp: 1.323 ± 0.034
1.982LeuTyr: 1.982 ± 0.035
0.0LeuXaa: 0.0 ± 0.0
Met
2.945MetAla: 2.945 ± 0.042
0.176MetCys: 0.176 ± 0.01
1.034MetAsp: 1.034 ± 0.025
1.043MetGlu: 1.043 ± 0.025
0.713MetPhe: 0.713 ± 0.021
1.742MetGly: 1.742 ± 0.035
0.561MetHis: 0.561 ± 0.019
0.929MetIle: 0.929 ± 0.024
1.065MetLys: 1.065 ± 0.026
2.669MetLeu: 2.669 ± 0.042
0.52MetMet: 0.52 ± 0.019
0.847MetAsn: 0.847 ± 0.019
1.548MetPro: 1.548 ± 0.029
1.031MetGln: 1.031 ± 0.024
1.782MetArg: 1.782 ± 0.033
1.603MetSer: 1.603 ± 0.029
1.519MetThr: 1.519 ± 0.031
1.696MetVal: 1.696 ± 0.031
0.202MetTrp: 0.202 ± 0.01
0.36MetTyr: 0.36 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
3.305AsnAla: 3.305 ± 0.049
0.241AsnCys: 0.241 ± 0.012
1.293AsnAsp: 1.293 ± 0.033
1.23AsnGlu: 1.23 ± 0.029
0.963AsnPhe: 0.963 ± 0.022
2.279AsnGly: 2.279 ± 0.049
0.494AsnHis: 0.494 ± 0.019
1.174AsnIle: 1.174 ± 0.033
0.787AsnLys: 0.787 ± 0.02
2.451AsnLeu: 2.451 ± 0.044
0.551AsnMet: 0.551 ± 0.018
0.724AsnAsn: 0.724 ± 0.027
1.755AsnPro: 1.755 ± 0.033
0.774AsnGln: 0.774 ± 0.025
1.576AsnArg: 1.576 ± 0.032
1.211AsnSer: 1.211 ± 0.032
1.373AsnThr: 1.373 ± 0.042
1.914AsnVal: 1.914 ± 0.036
0.408AsnTrp: 0.408 ± 0.016
0.668AsnTyr: 0.668 ± 0.02
0.0AsnXaa: 0.0 ± 0.0
Pro
7.597ProAla: 7.597 ± 0.083
0.364ProCys: 0.364 ± 0.017
3.011ProAsp: 3.011 ± 0.043
3.421ProGlu: 3.421 ± 0.05
1.848ProPhe: 1.848 ± 0.034
4.74ProGly: 4.74 ± 0.057
1.122ProHis: 1.122 ± 0.027
2.063ProIle: 2.063 ± 0.034
1.567ProLys: 1.567 ± 0.027
5.196ProLeu: 5.196 ± 0.056
1.309ProMet: 1.309 ± 0.028
1.3ProAsn: 1.3 ± 0.026
2.976ProPro: 2.976 ± 0.063
1.993ProGln: 1.993 ± 0.033
3.185ProArg: 3.185 ± 0.046
2.921ProSer: 2.921 ± 0.038
2.564ProThr: 2.564 ± 0.04
4.229ProVal: 4.229 ± 0.064
0.817ProTrp: 0.817 ± 0.024
1.199ProTyr: 1.199 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
5.046GlnAla: 5.046 ± 0.053
0.286GlnCys: 0.286 ± 0.014
1.433GlnAsp: 1.433 ± 0.033
1.473GlnGlu: 1.473 ± 0.028
1.196GlnPhe: 1.196 ± 0.022
2.789GlnGly: 2.789 ± 0.042
0.841GlnHis: 0.841 ± 0.024
1.662GlnIle: 1.662 ± 0.033
1.069GlnLys: 1.069 ± 0.024
4.146GlnLeu: 4.146 ± 0.049
0.925GlnMet: 0.925 ± 0.023
0.804GlnAsn: 0.804 ± 0.023
2.146GlnPro: 2.146 ± 0.039
1.845GlnGln: 1.845 ± 0.052
3.312GlnArg: 3.312 ± 0.055
1.942GlnSer: 1.942 ± 0.033
1.744GlnThr: 1.744 ± 0.034
2.73GlnVal: 2.73 ± 0.042
0.605GlnTrp: 0.605 ± 0.019
0.692GlnTyr: 0.692 ± 0.021
0.0GlnXaa: 0.0 ± 0.0
Arg
8.583ArgAla: 8.583 ± 0.085
0.641ArgCys: 0.641 ± 0.021
3.573ArgAsp: 3.573 ± 0.048
4.521ArgGlu: 4.521 ± 0.063
2.926ArgPhe: 2.926 ± 0.045
4.892ArgGly: 4.892 ± 0.058
1.851ArgHis: 1.851 ± 0.038
3.694ArgIle: 3.694 ± 0.046
2.142ArgLys: 2.142 ± 0.04
7.86ArgLeu: 7.86 ± 0.086
1.966ArgMet: 1.966 ± 0.029
1.794ArgAsn: 1.794 ± 0.034
3.425ArgPro: 3.425 ± 0.046
2.679ArgGln: 2.679 ± 0.04
5.544ArgArg: 5.544 ± 0.065
3.942ArgSer: 3.942 ± 0.056
3.402ArgThr: 3.402 ± 0.048
5.204ArgVal: 5.204 ± 0.063
1.265ArgTrp: 1.265 ± 0.032
1.81ArgTyr: 1.81 ± 0.033
0.0ArgXaa: 0.0 ± 0.0
Ser
6.806SerAla: 6.806 ± 0.065
0.454SerCys: 0.454 ± 0.017
2.53SerAsp: 2.53 ± 0.044
2.632SerGlu: 2.632 ± 0.041
2.29SerPhe: 2.29 ± 0.036
5.353SerGly: 5.353 ± 0.08
1.18SerHis: 1.18 ± 0.026
2.449SerIle: 2.449 ± 0.034
1.553SerLys: 1.553 ± 0.033
5.534SerLeu: 5.534 ± 0.063
1.419SerMet: 1.419 ± 0.025
1.432SerAsn: 1.432 ± 0.036
2.977SerPro: 2.977 ± 0.039
1.782SerGln: 1.782 ± 0.039
3.627SerArg: 3.627 ± 0.042
3.223SerSer: 3.223 ± 0.045
3.035SerThr: 3.035 ± 0.056
4.018SerVal: 4.018 ± 0.043
0.745SerTrp: 0.745 ± 0.019
1.331SerTyr: 1.331 ± 0.026
0.0SerXaa: 0.0 ± 0.0
Thr
6.004ThrAla: 6.004 ± 0.081
0.374ThrCys: 0.374 ± 0.016
2.438ThrAsp: 2.438 ± 0.044
2.333ThrGlu: 2.333 ± 0.036
1.624ThrPhe: 1.624 ± 0.033
4.704ThrGly: 4.704 ± 0.076
1.134ThrHis: 1.134 ± 0.026
2.164ThrIle: 2.164 ± 0.051
1.234ThrLys: 1.234 ± 0.031
6.16ThrLeu: 6.16 ± 0.077
1.102ThrMet: 1.102 ± 0.022
1.193ThrAsn: 1.193 ± 0.039
3.388ThrPro: 3.388 ± 0.048
1.888ThrGln: 1.888 ± 0.036
3.394ThrArg: 3.394 ± 0.042
2.711ThrSer: 2.711 ± 0.047
2.783ThrThr: 2.783 ± 0.061
4.259ThrVal: 4.259 ± 0.059
0.661ThrTrp: 0.661 ± 0.021
1.03ThrTyr: 1.03 ± 0.028
0.0ThrXaa: 0.0 ± 0.0
Val
9.785ValAla: 9.785 ± 0.089
0.723ValCys: 0.723 ± 0.025
4.03ValAsp: 4.03 ± 0.052
4.191ValGlu: 4.191 ± 0.055
2.73ValPhe: 2.73 ± 0.045
5.606ValGly: 5.606 ± 0.059
1.686ValHis: 1.686 ± 0.031
3.444ValIle: 3.444 ± 0.04
2.434ValLys: 2.434 ± 0.047
8.42ValLeu: 8.42 ± 0.085
1.799ValMet: 1.799 ± 0.029
2.035ValAsn: 2.035 ± 0.039
4.204ValPro: 4.204 ± 0.051
2.748ValGln: 2.748 ± 0.041
5.416ValArg: 5.416 ± 0.059
4.013ValSer: 4.013 ± 0.046
3.831ValThr: 3.831 ± 0.052
6.273ValVal: 6.273 ± 0.064
0.941ValTrp: 0.941 ± 0.025
1.52ValTyr: 1.52 ± 0.029
0.0ValXaa: 0.0 ± 0.0
Trp
1.388TrpAla: 1.388 ± 0.031
0.159TrpCys: 0.159 ± 0.009
0.625TrpAsp: 0.625 ± 0.021
0.547TrpGlu: 0.547 ± 0.019
0.575TrpPhe: 0.575 ± 0.019
0.963TrpGly: 0.963 ± 0.024
0.382TrpHis: 0.382 ± 0.016
0.704TrpIle: 0.704 ± 0.022
0.476TrpLys: 0.476 ± 0.018
2.022TrpLeu: 2.022 ± 0.038
0.417TrpMet: 0.417 ± 0.017
0.433TrpAsn: 0.433 ± 0.018
0.736TrpPro: 0.736 ± 0.023
0.724TrpGln: 0.724 ± 0.021
1.3TrpArg: 1.3 ± 0.032
0.84TrpSer: 0.84 ± 0.023
0.722TrpThr: 0.722 ± 0.021
0.966TrpVal: 0.966 ± 0.026
0.275TrpTrp: 0.275 ± 0.013
0.283TrpTyr: 0.283 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.619TyrAla: 2.619 ± 0.039
0.228TyrCys: 0.228 ± 0.012
1.151TyrAsp: 1.151 ± 0.029
1.15TyrGlu: 1.15 ± 0.027
0.923TyrPhe: 0.923 ± 0.026
2.018TyrGly: 2.018 ± 0.037
0.414TyrHis: 0.414 ± 0.016
0.752TyrIle: 0.752 ± 0.019
0.672TyrLys: 0.672 ± 0.021
2.28TyrLeu: 2.28 ± 0.039
0.437TyrMet: 0.437 ± 0.015
0.575TyrAsn: 0.575 ± 0.02
1.099TyrPro: 1.099 ± 0.026
0.751TyrGln: 0.751 ± 0.021
1.717TyrArg: 1.717 ± 0.034
1.143TyrSer: 1.143 ± 0.027
1.109TyrThr: 1.109 ± 0.027
1.649TyrVal: 1.649 ± 0.034
0.386TyrTrp: 0.386 ± 0.017
0.558TyrTyr: 0.558 ± 0.02
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5481 proteins (1763937 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski