Amino acid dipepetide frequency for Fictibacillus aquaticus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.694AlaAla: 8.694 ± 0.131	0.56AlaCys: 0.56 ± 0.022	3.96AlaAsp: 3.96 ± 0.056	5.761AlaGlu: 5.761 ± 0.091	3.442AlaPhe: 3.442 ± 0.063	6.681AlaGly: 6.681 ± 0.091	1.361AlaHis: 1.361 ± 0.036	5.236AlaIle: 5.236 ± 0.086	4.68AlaLys: 4.68 ± 0.081	7.469AlaLeu: 7.469 ± 0.095	2.072AlaMet: 2.072 ± 0.054	2.456AlaAsn: 2.456 ± 0.049	2.4AlaPro: 2.4 ± 0.057	2.333AlaGln: 2.333 ± 0.049	2.869AlaArg: 2.869 ± 0.054	4.638AlaSer: 4.638 ± 0.063	2.959AlaThr: 2.959 ± 0.055	6.863AlaVal: 6.863 ± 0.085	0.642AlaTrp: 0.642 ± 0.024	2.395AlaTyr: 2.395 ± 0.046	0.001AlaXaa: 0.001 ± 0.001
Cys	0.497CysAla: 0.497 ± 0.02	0.113CysCys: 0.113 ± 0.01	0.399CysAsp: 0.399 ± 0.02	0.459CysGlu: 0.459 ± 0.022	0.317CysPhe: 0.317 ± 0.018	0.699CysGly: 0.699 ± 0.027	0.202CysHis: 0.202 ± 0.011	0.465CysIle: 0.465 ± 0.02	0.326CysLys: 0.326 ± 0.015	0.683CysLeu: 0.683 ± 0.025	0.182CysMet: 0.182 ± 0.012	0.239CysAsn: 0.239 ± 0.016	0.335CysPro: 0.335 ± 0.019	0.227CysGln: 0.227 ± 0.015	0.344CysArg: 0.344 ± 0.019	0.559CysSer: 0.559 ± 0.023	0.384CysThr: 0.384 ± 0.018	0.41CysVal: 0.41 ± 0.018	0.089CysTrp: 0.089 ± 0.008	0.262CysTyr: 0.262 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	3.481AspAla: 3.481 ± 0.058	0.386AspCys: 0.386 ± 0.018	2.379AspAsp: 2.379 ± 0.055	4.326AspGlu: 4.326 ± 0.066	2.297AspPhe: 2.297 ± 0.05	3.775AspGly: 3.775 ± 0.075	1.156AspHis: 1.156 ± 0.033	3.729AspIle: 3.729 ± 0.065	3.128AspLys: 3.128 ± 0.062	4.792AspLeu: 4.792 ± 0.073	1.428AspMet: 1.428 ± 0.032	1.767AspAsn: 1.767 ± 0.045	1.943AspPro: 1.943 ± 0.04	1.691AspGln: 1.691 ± 0.038	2.281AspArg: 2.281 ± 0.044	2.94AspSer: 2.94 ± 0.053	2.371AspThr: 2.371 ± 0.045	3.778AspVal: 3.778 ± 0.058	0.604AspTrp: 0.604 ± 0.024	2.129AspTyr: 2.129 ± 0.05	0.001AspXaa: 0.001 ± 0.001
Glu	5.699GluAla: 5.699 ± 0.088	0.385GluCys: 0.385 ± 0.02	3.754GluAsp: 3.754 ± 0.073	6.695GluGlu: 6.695 ± 0.113	2.604GluPhe: 2.604 ± 0.046	4.629GluGly: 4.629 ± 0.062	1.532GluHis: 1.532 ± 0.036	5.079GluIle: 5.079 ± 0.08	6.794GluLys: 6.794 ± 0.101	7.157GluLeu: 7.157 ± 0.096	2.288GluMet: 2.288 ± 0.05	3.626GluAsn: 3.626 ± 0.057	1.966GluPro: 1.966 ± 0.051	3.04GluGln: 3.04 ± 0.065	3.567GluArg: 3.567 ± 0.063	3.701GluSer: 3.701 ± 0.061	3.951GluThr: 3.951 ± 0.066	4.603GluVal: 4.603 ± 0.073	0.861GluTrp: 0.861 ± 0.031	2.308GluTyr: 2.308 ± 0.047	0.001GluXaa: 0.001 ± 0.001
Phe	3.312PheAla: 3.312 ± 0.063	0.368PheCys: 0.368 ± 0.017	2.197PheAsp: 2.197 ± 0.046	2.789PheGlu: 2.789 ± 0.051	2.344PhePhe: 2.344 ± 0.064	3.302PheGly: 3.302 ± 0.059	0.985PheHis: 0.985 ± 0.033	3.504PheIle: 3.504 ± 0.061	2.556PheLys: 2.556 ± 0.059	4.53PheLeu: 4.53 ± 0.079	1.254PheMet: 1.254 ± 0.036	1.782PheAsn: 1.782 ± 0.04	1.708PhePro: 1.708 ± 0.034	1.497PheGln: 1.497 ± 0.043	1.528PheArg: 1.528 ± 0.039	3.523PheSer: 3.523 ± 0.054	2.712PheThr: 2.712 ± 0.058	3.034PheVal: 3.034 ± 0.055	0.548PheTrp: 0.548 ± 0.025	1.791PheTyr: 1.791 ± 0.045	0.001PheXaa: 0.001 ± 0.001
Gly	5.177GlyAla: 5.177 ± 0.097	0.644GlyCys: 0.644 ± 0.023	3.32GlyAsp: 3.32 ± 0.055	4.626GlyGlu: 4.626 ± 0.075	3.467GlyPhe: 3.467 ± 0.058	5.164GlyGly: 5.164 ± 0.099	1.37GlyHis: 1.37 ± 0.037	5.795GlyIle: 5.795 ± 0.091	5.298GlyLys: 5.298 ± 0.07	6.733GlyLeu: 6.733 ± 0.091	2.355GlyMet: 2.355 ± 0.059	2.71GlyAsn: 2.71 ± 0.064	1.789GlyPro: 1.789 ± 0.04	2.161GlyGln: 2.161 ± 0.054	2.939GlyArg: 2.939 ± 0.056	4.562GlySer: 4.562 ± 0.064	4.534GlyThr: 4.534 ± 0.092	5.108GlyVal: 5.108 ± 0.079	0.874GlyTrp: 0.874 ± 0.032	2.779GlyTyr: 2.779 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	1.411HisAla: 1.411 ± 0.036	0.223HisCys: 0.223 ± 0.014	1.09HisAsp: 1.09 ± 0.032	1.47HisGlu: 1.47 ± 0.034	1.115HisPhe: 1.115 ± 0.036	1.4HisGly: 1.4 ± 0.039	0.685HisHis: 0.685 ± 0.025	1.43HisIle: 1.43 ± 0.038	1.123HisLys: 1.123 ± 0.029	2.008HisLeu: 2.008 ± 0.043	0.574HisMet: 0.574 ± 0.023	0.792HisAsn: 0.792 ± 0.024	1.126HisPro: 1.126 ± 0.03	0.72HisGln: 0.72 ± 0.028	0.875HisArg: 0.875 ± 0.027	1.277HisSer: 1.277 ± 0.036	1.069HisThr: 1.069 ± 0.03	1.449HisVal: 1.449 ± 0.035	0.208HisTrp: 0.208 ± 0.015	0.883HisTyr: 0.883 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	5.9IleAla: 5.9 ± 0.07	0.546IleCys: 0.546 ± 0.022	3.664IleAsp: 3.664 ± 0.063	4.964IleGlu: 4.964 ± 0.081	2.928IlePhe: 2.928 ± 0.062	5.556IleGly: 5.556 ± 0.078	1.5IleHis: 1.5 ± 0.036	4.979IleIle: 4.979 ± 0.076	4.245IleLys: 4.245 ± 0.062	6.568IleLeu: 6.568 ± 0.1	1.807IleMet: 1.807 ± 0.041	2.731IleAsn: 2.731 ± 0.052	3.025IlePro: 3.025 ± 0.056	2.621IleGln: 2.621 ± 0.042	2.852IleArg: 2.852 ± 0.056	4.921IleSer: 4.921 ± 0.076	4.231IleThr: 4.231 ± 0.064	5.029IleVal: 5.029 ± 0.072	0.64IleTrp: 0.64 ± 0.028	2.146IleTyr: 2.146 ± 0.047	0.002IleXaa: 0.002 ± 0.001
Lys	5.14LysAla: 5.14 ± 0.087	0.327LysCys: 0.327 ± 0.02	3.874LysAsp: 3.874 ± 0.065	6.723LysGlu: 6.723 ± 0.098	1.856LysPhe: 1.856 ± 0.038	4.696LysGly: 4.696 ± 0.08	1.396LysHis: 1.396 ± 0.032	4.259LysIle: 4.259 ± 0.07	6.416LysLys: 6.416 ± 0.091	5.955LysLeu: 5.955 ± 0.084	2.249LysMet: 2.249 ± 0.045	3.388LysAsn: 3.388 ± 0.056	2.495LysPro: 2.495 ± 0.05	2.815LysGln: 2.815 ± 0.057	3.222LysArg: 3.222 ± 0.058	3.665LysSer: 3.665 ± 0.069	3.898LysThr: 3.898 ± 0.063	4.556LysVal: 4.556 ± 0.069	0.87LysTrp: 0.87 ± 0.033	2.001LysTyr: 2.001 ± 0.046	0.001LysXaa: 0.001 ± 0.001
Leu	7.713LeuAla: 7.713 ± 0.097	0.69LeuCys: 0.69 ± 0.023	4.677LeuAsp: 4.677 ± 0.069	6.243LeuGlu: 6.243 ± 0.091	4.951LeuPhe: 4.951 ± 0.085	6.319LeuGly: 6.319 ± 0.095	2.016LeuHis: 2.016 ± 0.043	6.687LeuIle: 6.687 ± 0.098	6.903LeuLys: 6.903 ± 0.096	10.287LeuLeu: 10.287 ± 0.147	2.582LeuMet: 2.582 ± 0.051	4.139LeuAsn: 4.139 ± 0.061	4.055LeuPro: 4.055 ± 0.068	3.638LeuGln: 3.638 ± 0.062	3.608LeuArg: 3.608 ± 0.058	7.212LeuSer: 7.212 ± 0.083	5.418LeuThr: 5.418 ± 0.083	5.969LeuVal: 5.969 ± 0.076	0.873LeuTrp: 0.873 ± 0.031	3.204LeuTyr: 3.204 ± 0.063	0.0LeuXaa: 0.0 ± 0.0
Met	2.243MetAla: 2.243 ± 0.046	0.161MetCys: 0.161 ± 0.012	1.529MetAsp: 1.529 ± 0.04	2.088MetGlu: 2.088 ± 0.039	1.135MetPhe: 1.135 ± 0.035	1.802MetGly: 1.802 ± 0.048	0.476MetHis: 0.476 ± 0.021	2.056MetIle: 2.056 ± 0.045	2.645MetLys: 2.645 ± 0.05	2.839MetLeu: 2.839 ± 0.053	0.967MetMet: 0.967 ± 0.033	1.629MetAsn: 1.629 ± 0.039	1.18MetPro: 1.18 ± 0.052	0.961MetGln: 0.961 ± 0.028	1.155MetArg: 1.155 ± 0.033	1.633MetSer: 1.633 ± 0.043	1.758MetThr: 1.758 ± 0.038	1.766MetVal: 1.766 ± 0.037	0.204MetTrp: 0.204 ± 0.014	0.733MetTyr: 0.733 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	2.865AsnAla: 2.865 ± 0.057	0.296AsnCys: 0.296 ± 0.016	2.062AsnAsp: 2.062 ± 0.049	3.314AsnGlu: 3.314 ± 0.051	1.481AsnPhe: 1.481 ± 0.041	3.341AsnGly: 3.341 ± 0.068	0.918AsnHis: 0.918 ± 0.027	2.878AsnIle: 2.878 ± 0.055	2.801AsnLys: 2.801 ± 0.058	3.381AsnLeu: 3.381 ± 0.06	1.201AsnMet: 1.201 ± 0.033	1.723AsnAsn: 1.723 ± 0.051	1.949AsnPro: 1.949 ± 0.043	1.363AsnGln: 1.363 ± 0.035	1.893AsnArg: 1.893 ± 0.041	2.419AsnSer: 2.419 ± 0.055	2.037AsnThr: 2.037 ± 0.043	3.023AsnVal: 3.023 ± 0.051	0.504AsnTrp: 0.504 ± 0.022	1.365AsnTyr: 1.365 ± 0.043	0.003AsnXaa: 0.003 ± 0.002
Pro	3.03ProAla: 3.03 ± 0.065	0.217ProCys: 0.217 ± 0.015	2.247ProAsp: 2.247 ± 0.051	3.226ProGlu: 3.226 ± 0.061	2.104ProPhe: 2.104 ± 0.044	2.63ProGly: 2.63 ± 0.066	0.823ProHis: 0.823 ± 0.023	2.264ProIle: 2.264 ± 0.05	2.069ProLys: 2.069 ± 0.045	3.535ProLeu: 3.535 ± 0.059	0.815ProMet: 0.815 ± 0.025	1.32ProAsn: 1.32 ± 0.037	1.1ProPro: 1.1 ± 0.04	1.188ProGln: 1.188 ± 0.045	1.123ProArg: 1.123 ± 0.03	2.369ProSer: 2.369 ± 0.045	1.491ProThr: 1.491 ± 0.041	3.383ProVal: 3.383 ± 0.067	0.396ProTrp: 0.396 ± 0.021	1.426ProTyr: 1.426 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	2.43GlnAla: 2.43 ± 0.049	0.208GlnCys: 0.208 ± 0.015	1.621GlnAsp: 1.621 ± 0.04	2.682GlnGlu: 2.682 ± 0.062	1.47GlnPhe: 1.47 ± 0.035	2.03GlnGly: 2.03 ± 0.041	0.826GlnHis: 0.826 ± 0.029	2.216GlnIle: 2.216 ± 0.049	2.795GlnLys: 2.795 ± 0.06	3.675GlnLeu: 3.675 ± 0.062	1.173GlnMet: 1.173 ± 0.039	1.676GlnAsn: 1.676 ± 0.045	1.291GlnPro: 1.291 ± 0.036	1.744GlnGln: 1.744 ± 0.061	1.48GlnArg: 1.48 ± 0.04	2.13GlnSer: 2.13 ± 0.045	1.889GlnThr: 1.889 ± 0.041	2.083GlnVal: 2.083 ± 0.045	0.397GlnTrp: 0.397 ± 0.02	1.323GlnTyr: 1.323 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	2.666ArgAla: 2.666 ± 0.052	0.279ArgCys: 0.279 ± 0.016	2.155ArgAsp: 2.155 ± 0.044	3.195ArgGlu: 3.195 ± 0.061	1.982ArgPhe: 1.982 ± 0.045	2.461ArgGly: 2.461 ± 0.052	0.865ArgHis: 0.865 ± 0.026	3.064ArgIle: 3.064 ± 0.048	3.159ArgLys: 3.159 ± 0.061	3.978ArgLeu: 3.978 ± 0.07	1.354ArgMet: 1.354 ± 0.035	1.769ArgAsn: 1.769 ± 0.043	1.355ArgPro: 1.355 ± 0.038	1.617ArgGln: 1.617 ± 0.04	1.969ArgArg: 1.969 ± 0.054	2.432ArgSer: 2.432 ± 0.049	2.187ArgThr: 2.187 ± 0.045	2.544ArgVal: 2.544 ± 0.05	0.454ArgTrp: 0.454 ± 0.022	1.554ArgTyr: 1.554 ± 0.036	0.001ArgXaa: 0.001 ± 0.001
Ser	4.747SerAla: 4.747 ± 0.081	0.492SerCys: 0.492 ± 0.021	3.05SerAsp: 3.05 ± 0.054	4.394SerGlu: 4.394 ± 0.078	3.386SerPhe: 3.386 ± 0.07	5.126SerGly: 5.126 ± 0.083	1.258SerHis: 1.258 ± 0.031	4.717SerIle: 4.717 ± 0.074	3.844SerLys: 3.844 ± 0.073	6.517SerLeu: 6.517 ± 0.094	1.837SerMet: 1.837 ± 0.044	2.127SerAsn: 2.127 ± 0.046	2.264SerPro: 2.264 ± 0.05	2.139SerGln: 2.139 ± 0.045	2.652SerArg: 2.652 ± 0.053	4.62SerSer: 4.62 ± 0.078	2.952SerThr: 2.952 ± 0.056	4.593SerVal: 4.593 ± 0.072	0.709SerTrp: 0.709 ± 0.024	2.404SerTyr: 2.404 ± 0.059	0.001SerXaa: 0.001 ± 0.001
Thr	4.784ThrAla: 4.784 ± 0.08	0.33ThrCys: 0.33 ± 0.018	2.704ThrAsp: 2.704 ± 0.058	3.596ThrGlu: 3.596 ± 0.059	2.617ThrPhe: 2.617 ± 0.06	4.618ThrGly: 4.618 ± 0.082	1.007ThrHis: 1.007 ± 0.028	3.848ThrIle: 3.848 ± 0.065	3.058ThrLys: 3.058 ± 0.053	5.128ThrLeu: 5.128 ± 0.065	1.326ThrMet: 1.326 ± 0.037	1.986ThrAsn: 1.986 ± 0.043	2.238ThrPro: 2.238 ± 0.045	1.305ThrGln: 1.305 ± 0.035	1.778ThrArg: 1.778 ± 0.04	3.225ThrSer: 3.225 ± 0.06	2.489ThrThr: 2.489 ± 0.057	4.529ThrVal: 4.529 ± 0.086	0.514ThrTrp: 0.514 ± 0.02	1.859ThrTyr: 1.859 ± 0.043	0.0ThrXaa: 0.0 ± 0.0
Val	4.748ValAla: 4.748 ± 0.072	0.59ValCys: 0.59 ± 0.025	3.25ValAsp: 3.25 ± 0.054	4.375ValGlu: 4.375 ± 0.065	3.373ValPhe: 3.373 ± 0.059	3.963ValGly: 3.963 ± 0.072	1.491ValHis: 1.491 ± 0.042	5.53ValIle: 5.53 ± 0.076	5.056ValLys: 5.056 ± 0.075	7.443ValLeu: 7.443 ± 0.092	2.171ValMet: 2.171 ± 0.047	2.986ValAsn: 2.986 ± 0.056	2.938ValPro: 2.938 ± 0.053	2.498ValGln: 2.498 ± 0.052	2.778ValArg: 2.778 ± 0.049	4.963ValSer: 4.963 ± 0.075	4.272ValThr: 4.272 ± 0.086	4.692ValVal: 4.692 ± 0.076	0.717ValTrp: 0.717 ± 0.025	2.493ValTyr: 2.493 ± 0.05	0.001ValXaa: 0.001 ± 0.001
Trp	0.688TrpAla: 0.688 ± 0.028	0.075TrpCys: 0.075 ± 0.008	0.543TrpAsp: 0.543 ± 0.026	0.701TrpGlu: 0.701 ± 0.024	0.527TrpPhe: 0.527 ± 0.023	0.667TrpGly: 0.667 ± 0.025	0.238TrpHis: 0.238 ± 0.014	0.813TrpIle: 0.813 ± 0.029	0.767TrpLys: 0.767 ± 0.03	1.2TrpLeu: 1.2 ± 0.034	0.387TrpMet: 0.387 ± 0.018	0.572TrpAsn: 0.572 ± 0.026	0.278TrpPro: 0.278 ± 0.017	0.356TrpGln: 0.356 ± 0.019	0.444TrpArg: 0.444 ± 0.019	0.695TrpSer: 0.695 ± 0.027	0.561TrpThr: 0.561 ± 0.026	0.67TrpVal: 0.67 ± 0.025	0.16TrpTrp: 0.16 ± 0.012	0.332TrpTyr: 0.332 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.247TyrAla: 2.247 ± 0.042	0.302TyrCys: 0.302 ± 0.017	1.901TyrAsp: 1.901 ± 0.053	2.611TyrGlu: 2.611 ± 0.051	1.868TyrPhe: 1.868 ± 0.048	2.547TyrGly: 2.547 ± 0.049	0.822TyrHis: 0.822 ± 0.027	2.306TyrIle: 2.306 ± 0.048	2.142TyrLys: 2.142 ± 0.041	3.305TyrLeu: 3.305 ± 0.057	0.935TyrMet: 0.935 ± 0.032	1.396TyrAsn: 1.396 ± 0.04	1.322TyrPro: 1.322 ± 0.04	1.224TyrGln: 1.224 ± 0.037	1.684TyrArg: 1.684 ± 0.042	2.344TyrSer: 2.344 ± 0.057	1.775TyrThr: 1.775 ± 0.043	2.245TyrVal: 2.245 ± 0.049	0.413TyrTrp: 0.413 ± 0.021	1.39TyrTyr: 1.39 ± 0.047	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.002XaaAsp: 0.002 ± 0.001	0.002XaaGlu: 0.002 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.001XaaAsn: 0.001 ± 0.001	0.001XaaPro: 0.001 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.004XaaXaa: 0.004 ± 0.002

Statistics based on 3939 proteins (1115026 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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