Tortoise microvirus 49

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W6G4|A0A4P8W6G4_9VIRU Replication initiation protein OS=Tortoise microvirus 49 OX=2583153 PE=4 SV=1
MM1 pKa = 7.42EE2 pKa = 4.0VFKK5 pKa = 11.23RR6 pKa = 11.84FVNEE10 pKa = 3.66FNTMDD15 pKa = 3.34DD16 pKa = 3.98HH17 pKa = 7.45LRR19 pKa = 11.84KK20 pKa = 8.54TSMTVPDD27 pKa = 4.65DD28 pKa = 3.63SLSIRR33 pKa = 11.84EE34 pKa = 4.08LYY36 pKa = 9.17EE37 pKa = 4.33RR38 pKa = 11.84YY39 pKa = 8.31VTGALDD45 pKa = 3.47QSLAMSNARR54 pKa = 11.84AEE56 pKa = 4.31YY57 pKa = 10.45DD58 pKa = 3.57GEE60 pKa = 4.43NPDD63 pKa = 3.9FDD65 pKa = 6.2DD66 pKa = 3.95YY67 pKa = 11.59TPSLDD72 pKa = 3.49AVSAYY77 pKa = 7.47EE78 pKa = 3.98TGIMLQEE85 pKa = 3.61NRR87 pKa = 11.84MRR89 pKa = 11.84AEE91 pKa = 3.74QEE93 pKa = 3.78AQYY96 pKa = 10.71LASKK100 pKa = 8.43QQEE103 pKa = 4.3NEE105 pKa = 3.93GVQMDD110 pKa = 3.98RR111 pKa = 11.84QSPKK115 pKa = 10.37SSSGDD120 pKa = 3.21SGNSGKK126 pKa = 10.56GVSGEE131 pKa = 4.19GG132 pKa = 3.25

Molecular weight: 14.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W6A8|A0A4P8W6A8_9VIRU Uncharacterized protein OS=Tortoise microvirus 49 OX=2583153 PE=4 SV=1
MM1 pKa = 7.82DD2 pKa = 4.62RR3 pKa = 11.84AFVLIDD9 pKa = 4.2KK10 pKa = 8.26EE11 pKa = 4.45CQSAAASRR19 pKa = 11.84LTPLHH24 pKa = 6.48ASEE27 pKa = 4.17APRR30 pKa = 11.84RR31 pKa = 11.84CRR33 pKa = 11.84EE34 pKa = 3.44RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 10.5CRR39 pKa = 11.84TCARR43 pKa = 11.84AVSRR47 pKa = 11.84RR48 pKa = 11.84LTRR51 pKa = 11.84KK52 pKa = 8.89HH53 pKa = 5.17QFCDD57 pKa = 3.71EE58 pKa = 4.25VAGTVSTEE66 pKa = 3.7RR67 pKa = 11.84SVLARR72 pKa = 11.84NFWSARR78 pKa = 11.84SVSLYY83 pKa = 10.49VDD85 pKa = 2.73KK86 pKa = 11.01CGYY89 pKa = 9.64KK90 pKa = 9.65ICLKK94 pKa = 10.54HH95 pKa = 6.33

Molecular weight: 10.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1592

95

563

265.3

30.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.035 ± 1.471

1.445 ± 0.682

5.842 ± 0.767

5.465 ± 0.679

5.528 ± 0.831

6.093 ± 1.086

1.759 ± 0.461

4.648 ± 0.541

5.842 ± 1.072

7.538 ± 0.647

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.827 ± 0.42

5.653 ± 0.81

4.146 ± 0.961

4.46 ± 1.0

6.407 ± 0.861

7.538 ± 1.069

5.339 ± 0.614

5.967 ± 1.238

1.193 ± 0.224

5.276 ± 1.077
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski