Amino acid dipepetide frequency for Mycobacterium phage C3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.754AlaAla: 9.754 ± 1.086
0.51AlaCys: 0.51 ± 0.15
6.248AlaAsp: 6.248 ± 0.616
7.714AlaGlu: 7.714 ± 0.846
4.271AlaPhe: 4.271 ± 0.535
7.905AlaGly: 7.905 ± 0.976
1.466AlaHis: 1.466 ± 0.336
4.144AlaIle: 4.144 ± 0.667
4.654AlaLys: 4.654 ± 0.486
8.925AlaLeu: 8.925 ± 0.848
3.251AlaMet: 3.251 ± 0.361
3.443AlaAsn: 3.443 ± 0.403
4.654AlaPro: 4.654 ± 0.699
3.443AlaGln: 3.443 ± 0.522
6.248AlaArg: 6.248 ± 0.757
4.59AlaSer: 4.59 ± 0.509
4.399AlaThr: 4.399 ± 0.595
7.076AlaVal: 7.076 ± 0.624
2.359AlaTrp: 2.359 ± 0.373
2.55AlaTyr: 2.55 ± 0.487
0.0AlaXaa: 0.0 ± 0.0
Cys
0.701CysAla: 0.701 ± 0.233
0.128CysCys: 0.128 ± 0.125
0.765CysAsp: 0.765 ± 0.23
0.446CysGlu: 0.446 ± 0.137
0.255CysPhe: 0.255 ± 0.124
0.638CysGly: 0.638 ± 0.196
0.191CysHis: 0.191 ± 0.108
0.319CysIle: 0.319 ± 0.136
0.446CysLys: 0.446 ± 0.155
0.638CysLeu: 0.638 ± 0.183
0.191CysMet: 0.191 ± 0.1
0.446CysAsn: 0.446 ± 0.148
0.638CysPro: 0.638 ± 0.254
0.319CysGln: 0.319 ± 0.134
0.829CysArg: 0.829 ± 0.24
0.574CysSer: 0.574 ± 0.204
0.446CysThr: 0.446 ± 0.18
0.638CysVal: 0.638 ± 0.191
0.383CysTrp: 0.383 ± 0.164
0.51CysTyr: 0.51 ± 0.186
0.0CysXaa: 0.0 ± 0.0
Asp
5.546AspAla: 5.546 ± 0.554
0.829AspCys: 0.829 ± 0.225
3.889AspAsp: 3.889 ± 0.521
4.718AspGlu: 4.718 ± 0.654
2.869AspPhe: 2.869 ± 0.491
6.184AspGly: 6.184 ± 0.542
1.658AspHis: 1.658 ± 0.354
3.251AspIle: 3.251 ± 0.461
2.231AspLys: 2.231 ± 0.347
4.718AspLeu: 4.718 ± 0.565
1.339AspMet: 1.339 ± 0.291
1.849AspAsn: 1.849 ± 0.42
5.228AspPro: 5.228 ± 0.476
1.976AspGln: 1.976 ± 0.285
3.57AspArg: 3.57 ± 0.42
3.124AspSer: 3.124 ± 0.398
3.443AspThr: 3.443 ± 0.499
4.335AspVal: 4.335 ± 0.62
1.403AspTrp: 1.403 ± 0.284
2.614AspTyr: 2.614 ± 0.414
0.0AspXaa: 0.0 ± 0.0
Glu
8.543GluAla: 8.543 ± 1.03
0.383GluCys: 0.383 ± 0.193
4.59GluAsp: 4.59 ± 0.545
5.355GluGlu: 5.355 ± 0.88
3.379GluPhe: 3.379 ± 0.371
4.59GluGly: 4.59 ± 0.634
1.721GluHis: 1.721 ± 0.33
4.08GluIle: 4.08 ± 0.472
2.295GluLys: 2.295 ± 0.302
6.63GluLeu: 6.63 ± 0.647
2.168GluMet: 2.168 ± 0.387
1.849GluAsn: 1.849 ± 0.325
3.188GluPro: 3.188 ± 0.435
2.231GluGln: 2.231 ± 0.31
4.654GluArg: 4.654 ± 0.527
2.933GluSer: 2.933 ± 0.444
3.953GluThr: 3.953 ± 0.442
4.463GluVal: 4.463 ± 0.532
1.275GluTrp: 1.275 ± 0.291
2.04GluTyr: 2.04 ± 0.432
0.0GluXaa: 0.0 ± 0.0
Phe
2.996PheAla: 2.996 ± 0.556
0.446PheCys: 0.446 ± 0.154
2.805PheAsp: 2.805 ± 0.539
2.614PheGlu: 2.614 ± 0.384
0.893PhePhe: 0.893 ± 0.238
2.933PheGly: 2.933 ± 0.451
0.446PheHis: 0.446 ± 0.157
1.148PheIle: 1.148 ± 0.287
1.53PheLys: 1.53 ± 0.285
2.741PheLeu: 2.741 ± 0.458
0.446PheMet: 0.446 ± 0.161
1.275PheAsn: 1.275 ± 0.332
1.721PhePro: 1.721 ± 0.309
1.275PheGln: 1.275 ± 0.333
2.295PheArg: 2.295 ± 0.323
2.486PheSer: 2.486 ± 0.426
2.869PheThr: 2.869 ± 0.393
2.486PheVal: 2.486 ± 0.36
0.574PheTrp: 0.574 ± 0.191
0.829PheTyr: 0.829 ± 0.195
0.0PheXaa: 0.0 ± 0.0
Gly
6.375GlyAla: 6.375 ± 0.905
0.829GlyCys: 0.829 ± 0.232
5.993GlyAsp: 5.993 ± 0.726
4.526GlyGlu: 4.526 ± 0.607
2.996GlyPhe: 2.996 ± 0.48
9.053GlyGly: 9.053 ± 1.889
2.295GlyHis: 2.295 ± 0.377
4.335GlyIle: 4.335 ± 0.709
3.825GlyLys: 3.825 ± 0.535
6.885GlyLeu: 6.885 ± 0.867
1.658GlyMet: 1.658 ± 0.227
3.124GlyAsn: 3.124 ± 0.486
3.315GlyPro: 3.315 ± 0.415
3.506GlyGln: 3.506 ± 0.434
3.953GlyArg: 3.953 ± 0.471
3.57GlySer: 3.57 ± 0.519
5.164GlyThr: 5.164 ± 0.607
5.929GlyVal: 5.929 ± 0.713
1.658GlyTrp: 1.658 ± 0.3
2.359GlyTyr: 2.359 ± 0.352
0.0GlyXaa: 0.0 ± 0.0
His
1.594HisAla: 1.594 ± 0.323
0.319HisCys: 0.319 ± 0.116
1.594HisAsp: 1.594 ± 0.313
1.658HisGlu: 1.658 ± 0.409
0.574HisPhe: 0.574 ± 0.182
2.104HisGly: 2.104 ± 0.45
0.574HisHis: 0.574 ± 0.17
1.403HisIle: 1.403 ± 0.29
1.084HisLys: 1.084 ± 0.275
1.211HisLeu: 1.211 ± 0.251
0.319HisMet: 0.319 ± 0.135
0.51HisAsn: 0.51 ± 0.202
1.211HisPro: 1.211 ± 0.261
0.893HisGln: 0.893 ± 0.229
1.721HisArg: 1.721 ± 0.415
0.765HisSer: 0.765 ± 0.192
1.211HisThr: 1.211 ± 0.319
1.849HisVal: 1.849 ± 0.338
0.383HisTrp: 0.383 ± 0.156
0.383HisTyr: 0.383 ± 0.215
0.0HisXaa: 0.0 ± 0.0
Ile
4.526IleAla: 4.526 ± 0.416
0.383IleCys: 0.383 ± 0.178
3.379IleAsp: 3.379 ± 0.417
4.526IleGlu: 4.526 ± 0.507
0.893IlePhe: 0.893 ± 0.274
4.144IleGly: 4.144 ± 0.619
1.594IleHis: 1.594 ± 0.261
1.849IleIle: 1.849 ± 0.34
2.741IleLys: 2.741 ± 0.473
3.889IleLeu: 3.889 ± 0.473
0.638IleMet: 0.638 ± 0.201
1.785IleAsn: 1.785 ± 0.313
3.315IlePro: 3.315 ± 0.448
1.148IleGln: 1.148 ± 0.28
3.698IleArg: 3.698 ± 0.506
2.933IleSer: 2.933 ± 0.452
2.869IleThr: 2.869 ± 0.396
2.805IleVal: 2.805 ± 0.395
0.638IleTrp: 0.638 ± 0.165
0.893IleTyr: 0.893 ± 0.248
0.0IleXaa: 0.0 ± 0.0
Lys
4.781LysAla: 4.781 ± 0.711
0.383LysCys: 0.383 ± 0.163
2.231LysAsp: 2.231 ± 0.325
2.933LysGlu: 2.933 ± 0.427
0.829LysPhe: 0.829 ± 0.248
3.06LysGly: 3.06 ± 0.373
0.51LysHis: 0.51 ± 0.168
2.168LysIle: 2.168 ± 0.409
3.251LysLys: 3.251 ± 0.639
3.634LysLeu: 3.634 ± 0.577
1.466LysMet: 1.466 ± 0.325
1.913LysAsn: 1.913 ± 0.384
2.678LysPro: 2.678 ± 0.556
1.849LysGln: 1.849 ± 0.406
3.251LysArg: 3.251 ± 0.475
2.295LysSer: 2.295 ± 0.392
2.741LysThr: 2.741 ± 0.449
3.825LysVal: 3.825 ± 0.498
0.765LysTrp: 0.765 ± 0.207
1.084LysTyr: 1.084 ± 0.332
0.0LysXaa: 0.0 ± 0.0
Leu
8.861LeuAla: 8.861 ± 0.758
0.829LeuCys: 0.829 ± 0.26
4.335LeuAsp: 4.335 ± 0.503
5.164LeuGlu: 5.164 ± 0.624
2.55LeuPhe: 2.55 ± 0.354
6.885LeuGly: 6.885 ± 0.983
2.168LeuHis: 2.168 ± 0.413
4.463LeuIle: 4.463 ± 0.443
3.124LeuLys: 3.124 ± 0.393
6.184LeuLeu: 6.184 ± 0.583
2.678LeuMet: 2.678 ± 0.435
2.359LeuAsn: 2.359 ± 0.44
4.271LeuPro: 4.271 ± 0.558
2.869LeuGln: 2.869 ± 0.649
5.228LeuArg: 5.228 ± 0.687
4.973LeuSer: 4.973 ± 0.693
5.355LeuThr: 5.355 ± 0.771
5.291LeuVal: 5.291 ± 0.62
1.721LeuTrp: 1.721 ± 0.295
2.486LeuTyr: 2.486 ± 0.428
0.0LeuXaa: 0.0 ± 0.0
Met
3.251MetAla: 3.251 ± 0.512
0.191MetCys: 0.191 ± 0.105
0.893MetAsp: 0.893 ± 0.238
1.084MetGlu: 1.084 ± 0.214
0.383MetPhe: 0.383 ± 0.159
1.466MetGly: 1.466 ± 0.348
0.319MetHis: 0.319 ± 0.127
1.339MetIle: 1.339 ± 0.296
1.403MetLys: 1.403 ± 0.283
1.339MetLeu: 1.339 ± 0.237
0.638MetMet: 0.638 ± 0.176
1.084MetAsn: 1.084 ± 0.287
1.466MetPro: 1.466 ± 0.368
1.02MetGln: 1.02 ± 0.244
1.339MetArg: 1.339 ± 0.335
2.295MetSer: 2.295 ± 0.377
2.231MetThr: 2.231 ± 0.356
1.211MetVal: 1.211 ± 0.333
0.255MetTrp: 0.255 ± 0.112
0.893MetTyr: 0.893 ± 0.214
0.0MetXaa: 0.0 ± 0.0
Asn
3.889AsnAla: 3.889 ± 0.512
0.191AsnCys: 0.191 ± 0.097
2.231AsnAsp: 2.231 ± 0.348
2.231AsnGlu: 2.231 ± 0.345
0.956AsnPhe: 0.956 ± 0.244
3.379AsnGly: 3.379 ± 0.436
0.765AsnHis: 0.765 ± 0.22
1.53AsnIle: 1.53 ± 0.304
1.084AsnLys: 1.084 ± 0.276
3.124AsnLeu: 3.124 ± 0.41
1.084AsnMet: 1.084 ± 0.26
0.383AsnAsn: 0.383 ± 0.181
2.231AsnPro: 2.231 ± 0.402
0.638AsnGln: 0.638 ± 0.201
2.359AsnArg: 2.359 ± 0.431
1.275AsnSer: 1.275 ± 0.251
2.04AsnThr: 2.04 ± 0.413
2.231AsnVal: 2.231 ± 0.352
0.701AsnTrp: 0.701 ± 0.193
0.893AsnTyr: 0.893 ± 0.204
0.0AsnXaa: 0.0 ± 0.0
Pro
4.781ProAla: 4.781 ± 0.567
0.383ProCys: 0.383 ± 0.16
4.271ProAsp: 4.271 ± 0.476
3.761ProGlu: 3.761 ± 0.558
1.976ProPhe: 1.976 ± 0.431
4.463ProGly: 4.463 ± 0.718
1.148ProHis: 1.148 ± 0.292
2.359ProIle: 2.359 ± 0.386
2.486ProLys: 2.486 ± 0.462
3.57ProLeu: 3.57 ± 0.513
1.084ProMet: 1.084 ± 0.274
1.976ProAsn: 1.976 ± 0.383
2.168ProPro: 2.168 ± 0.485
0.956ProGln: 0.956 ± 0.232
3.825ProArg: 3.825 ± 0.604
2.741ProSer: 2.741 ± 0.339
3.443ProThr: 3.443 ± 0.46
4.59ProVal: 4.59 ± 0.569
1.466ProTrp: 1.466 ± 0.384
1.466ProTyr: 1.466 ± 0.277
0.0ProXaa: 0.0 ± 0.0
Gln
4.144GlnAla: 4.144 ± 0.579
0.128GlnCys: 0.128 ± 0.097
1.084GlnAsp: 1.084 ± 0.274
1.658GlnGlu: 1.658 ± 0.332
1.148GlnPhe: 1.148 ± 0.256
2.231GlnGly: 2.231 ± 0.329
0.829GlnHis: 0.829 ± 0.174
2.805GlnIle: 2.805 ± 0.42
1.466GlnLys: 1.466 ± 0.307
3.698GlnLeu: 3.698 ± 0.782
0.446GlnMet: 0.446 ± 0.169
0.638GlnAsn: 0.638 ± 0.191
1.403GlnPro: 1.403 ± 0.304
1.594GlnGln: 1.594 ± 0.342
3.124GlnArg: 3.124 ± 0.462
1.02GlnSer: 1.02 ± 0.238
2.295GlnThr: 2.295 ± 0.368
2.741GlnVal: 2.741 ± 0.378
0.765GlnTrp: 0.765 ± 0.245
1.403GlnTyr: 1.403 ± 0.387
0.0GlnXaa: 0.0 ± 0.0
Arg
5.546ArgAla: 5.546 ± 0.774
1.339ArgCys: 1.339 ± 0.324
4.526ArgAsp: 4.526 ± 0.545
5.674ArgGlu: 5.674 ± 0.707
2.614ArgPhe: 2.614 ± 0.493
3.825ArgGly: 3.825 ± 0.52
1.339ArgHis: 1.339 ± 0.339
3.379ArgIle: 3.379 ± 0.475
3.379ArgLys: 3.379 ± 0.48
6.248ArgLeu: 6.248 ± 0.587
1.658ArgMet: 1.658 ± 0.376
2.423ArgAsn: 2.423 ± 0.436
2.741ArgPro: 2.741 ± 0.435
1.721ArgGln: 1.721 ± 0.331
5.738ArgArg: 5.738 ± 0.675
3.379ArgSer: 3.379 ± 0.457
2.359ArgThr: 2.359 ± 0.312
4.654ArgVal: 4.654 ± 0.479
1.466ArgTrp: 1.466 ± 0.326
2.359ArgTyr: 2.359 ± 0.421
0.0ArgXaa: 0.0 ± 0.0
Ser
4.973SerAla: 4.973 ± 0.673
0.446SerCys: 0.446 ± 0.179
3.188SerAsp: 3.188 ± 0.408
3.698SerGlu: 3.698 ± 0.54
2.04SerPhe: 2.04 ± 0.344
4.335SerGly: 4.335 ± 0.698
0.765SerHis: 0.765 ± 0.199
1.976SerIle: 1.976 ± 0.378
2.04SerLys: 2.04 ± 0.354
3.761SerLeu: 3.761 ± 0.49
1.466SerMet: 1.466 ± 0.38
1.53SerAsn: 1.53 ± 0.264
2.996SerPro: 2.996 ± 0.42
2.805SerGln: 2.805 ± 0.413
3.953SerArg: 3.953 ± 0.487
3.188SerSer: 3.188 ± 0.557
2.933SerThr: 2.933 ± 0.335
3.825SerVal: 3.825 ± 0.48
1.594SerTrp: 1.594 ± 0.357
1.02SerTyr: 1.02 ± 0.283
0.0SerXaa: 0.0 ± 0.0
Thr
6.056ThrAla: 6.056 ± 0.558
0.638ThrCys: 0.638 ± 0.167
3.698ThrAsp: 3.698 ± 0.474
3.379ThrGlu: 3.379 ± 0.459
1.849ThrPhe: 1.849 ± 0.327
5.483ThrGly: 5.483 ± 0.636
1.211ThrHis: 1.211 ± 0.302
2.869ThrIle: 2.869 ± 0.451
4.016ThrLys: 4.016 ± 0.529
4.718ThrLeu: 4.718 ± 0.709
1.658ThrMet: 1.658 ± 0.302
1.658ThrAsn: 1.658 ± 0.283
4.08ThrPro: 4.08 ± 0.636
1.913ThrGln: 1.913 ± 0.323
2.741ThrArg: 2.741 ± 0.432
2.678ThrSer: 2.678 ± 0.453
2.486ThrThr: 2.486 ± 0.382
4.718ThrVal: 4.718 ± 0.545
1.02ThrTrp: 1.02 ± 0.239
1.594ThrTyr: 1.594 ± 0.292
0.0ThrXaa: 0.0 ± 0.0
Val
6.694ValAla: 6.694 ± 0.72
0.51ValCys: 0.51 ± 0.181
5.674ValAsp: 5.674 ± 0.615
5.291ValGlu: 5.291 ± 0.573
2.614ValPhe: 2.614 ± 0.479
5.164ValGly: 5.164 ± 0.701
1.658ValHis: 1.658 ± 0.357
2.678ValIle: 2.678 ± 0.425
3.188ValLys: 3.188 ± 0.483
5.674ValLeu: 5.674 ± 0.771
1.02ValMet: 1.02 ± 0.263
3.315ValAsn: 3.315 ± 0.475
3.315ValPro: 3.315 ± 0.489
2.168ValGln: 2.168 ± 0.483
4.718ValArg: 4.718 ± 0.612
4.399ValSer: 4.399 ± 0.57
4.654ValThr: 4.654 ± 0.585
6.184ValVal: 6.184 ± 0.533
1.658ValTrp: 1.658 ± 0.386
1.785ValTyr: 1.785 ± 0.295
0.0ValXaa: 0.0 ± 0.0
Trp
1.849TrpAla: 1.849 ± 0.348
0.51TrpCys: 0.51 ± 0.208
1.084TrpAsp: 1.084 ± 0.232
1.785TrpGlu: 1.785 ± 0.276
0.893TrpPhe: 0.893 ± 0.249
1.275TrpGly: 1.275 ± 0.276
0.446TrpHis: 0.446 ± 0.159
1.148TrpIle: 1.148 ± 0.254
0.701TrpLys: 0.701 ± 0.227
1.466TrpLeu: 1.466 ± 0.297
0.319TrpMet: 0.319 ± 0.135
0.701TrpAsn: 0.701 ± 0.208
0.893TrpPro: 0.893 ± 0.267
1.275TrpGln: 1.275 ± 0.265
1.148TrpArg: 1.148 ± 0.266
1.403TrpSer: 1.403 ± 0.253
1.785TrpThr: 1.785 ± 0.311
1.275TrpVal: 1.275 ± 0.265
0.446TrpTrp: 0.446 ± 0.204
0.701TrpTyr: 0.701 ± 0.268
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.741TyrAla: 2.741 ± 0.438
0.128TyrCys: 0.128 ± 0.087
2.295TyrAsp: 2.295 ± 0.381
2.104TyrGlu: 2.104 ± 0.382
0.829TyrPhe: 0.829 ± 0.203
2.231TyrGly: 2.231 ± 0.429
0.255TyrHis: 0.255 ± 0.126
1.403TyrIle: 1.403 ± 0.277
0.701TyrLys: 0.701 ± 0.201
2.741TyrLeu: 2.741 ± 0.434
0.51TyrMet: 0.51 ± 0.229
0.956TyrAsn: 0.956 ± 0.233
1.403TyrPro: 1.403 ± 0.262
1.02TyrGln: 1.02 ± 0.211
2.04TyrArg: 2.04 ± 0.418
1.913TyrSer: 1.913 ± 0.306
1.785TyrThr: 1.785 ± 0.355
2.231TyrVal: 2.231 ± 0.478
0.574TyrTrp: 0.574 ± 0.186
0.701TyrTyr: 0.701 ± 0.212
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 88 proteins (15687 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski