Enterococcus phage PEf771

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Brockvirinae; Schiekvirus; unclassified Schiekvirus

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 197 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C2H1A0|A0A5C2H1A0_9CAUD Uncharacterized protein OS=Enterococcus phage PEf771 OX=2601638 GN=PEf771_146 PE=4 SV=1
MM1 pKa = 7.82KK2 pKa = 10.73YY3 pKa = 8.7MAFYY7 pKa = 10.34IADD10 pKa = 3.88FLYY13 pKa = 9.25PDD15 pKa = 3.77EE16 pKa = 6.16RR17 pKa = 11.84IGDD20 pKa = 3.64LCFFYY25 pKa = 11.27NKK27 pKa = 8.02DD28 pKa = 3.2TNMFEE33 pKa = 4.37MACDD37 pKa = 3.74PEE39 pKa = 4.53TAYY42 pKa = 10.9DD43 pKa = 3.86PAVVCAMPEE52 pKa = 3.76FLLFKK57 pKa = 10.36VQGIEE62 pKa = 3.7EE63 pKa = 5.02DD64 pKa = 4.03GTIEE68 pKa = 4.58PVDD71 pKa = 3.2KK72 pKa = 10.89TKK74 pKa = 10.51FLEE77 pKa = 4.91EE78 pKa = 4.14INN80 pKa = 3.75

Molecular weight: 9.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C2H0H6|A0A5C2H0H6_9CAUD Homing endonuclease OS=Enterococcus phage PEf771 OX=2601638 GN=PEf771_140 PE=4 SV=1
MM1 pKa = 8.16RR2 pKa = 11.84YY3 pKa = 7.14TVEE6 pKa = 5.13KK7 pKa = 9.95GDD9 pKa = 3.62TMYY12 pKa = 10.94SIARR16 pKa = 11.84KK17 pKa = 9.92YY18 pKa = 9.54YY19 pKa = 8.84GKK21 pKa = 9.45PWLWRR26 pKa = 11.84LIRR29 pKa = 11.84LHH31 pKa = 5.69GQNRR35 pKa = 11.84SIKK38 pKa = 10.32DD39 pKa = 3.52PNKK42 pKa = 10.3LRR44 pKa = 11.84IGQTIYY50 pKa = 11.28LPFKK54 pKa = 10.69

Molecular weight: 6.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

197

0

197

44250

32

2198

224.6

25.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.138 ± 0.243

0.816 ± 0.081

6.341 ± 0.138

8.237 ± 0.309

3.831 ± 0.108

6.206 ± 0.238

1.602 ± 0.108

6.294 ± 0.153

7.738 ± 0.182

8.226 ± 0.202

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.581 ± 0.083

5.44 ± 0.149

3.182 ± 0.139

3.862 ± 0.182

4.298 ± 0.134

5.93 ± 0.165

6.538 ± 0.263

6.958 ± 0.184

1.114 ± 0.066

4.669 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski