Anopheles gambiae (African malaria mosquito)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Nematocera; Culicomorpha;

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17099 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F5HIZ0|F5HIZ0_ANOGA AGAP013444-PA OS=Anopheles gambiae OX=7165 GN=AgaP_AGAP013444 PE=4 SV=1
PP1 pKa = 7.84DD2 pKa = 5.44AEE4 pKa = 6.42DD5 pKa = 5.91DD6 pKa = 5.0DD7 pKa = 6.29DD8 pKa = 6.88DD9 pKa = 5.91NDD11 pKa = 4.27GVPDD15 pKa = 3.68SQEE18 pKa = 3.99TVIVPTAIVCADD30 pKa = 3.67ADD32 pKa = 4.07EE33 pKa = 4.61EE34 pKa = 5.4DD35 pKa = 5.05EE36 pKa = 4.28ICHH39 pKa = 6.43TYY41 pKa = 10.53SEE43 pKa = 5.23GIIQVKK49 pKa = 8.66ATFDD53 pKa = 3.17AHH55 pKa = 5.89NEE57 pKa = 3.73KK58 pKa = 11.01DD59 pKa = 3.54EE60 pKa = 4.51GDD62 pKa = 3.71EE63 pKa = 4.51CSYY66 pKa = 10.99PLDD69 pKa = 4.11GFRR72 pKa = 11.84CPLDD76 pKa = 3.72KK77 pKa = 10.94AWVKK81 pKa = 10.18AQPAGCKK88 pKa = 9.43PVVQCDD94 pKa = 2.92IADD97 pKa = 4.13PYY99 pKa = 11.06EE100 pKa = 4.21EE101 pKa = 4.83SEE103 pKa = 4.32SLLYY107 pKa = 9.41GTTCDD112 pKa = 4.4GDD114 pKa = 3.72EE115 pKa = 5.37DD116 pKa = 5.02NDD118 pKa = 4.12GDD120 pKa = 4.97DD121 pKa = 5.6DD122 pKa = 4.74EE123 pKa = 7.59GDD125 pKa = 3.67STFYY129 pKa = 10.86YY130 pKa = 10.13YY131 pKa = 11.24LSIRR135 pKa = 11.84SLLDD139 pKa = 3.27FFLLSALTLLNTIIVIVTRR158 pKa = 11.84DD159 pKa = 3.23RR160 pKa = 11.84TTSGVADD167 pKa = 5.09FGRR170 pKa = 11.84QLVWGAVGWIIFFNDD185 pKa = 4.38DD186 pKa = 3.36YY187 pKa = 10.29EE188 pKa = 4.41QPYY191 pKa = 9.62MLFVLLYY198 pKa = 6.42TTAAVILLSPLKK210 pKa = 9.63MDD212 pKa = 5.0LSPPEE217 pKa = 4.36EE218 pKa = 3.82WWQLRR223 pKa = 11.84TVPQKK228 pKa = 10.49FLSVPACRR236 pKa = 11.84KK237 pKa = 8.91YY238 pKa = 10.54FQRR241 pKa = 11.84CWLPFLVAIGLGSMWSVIDD260 pKa = 5.23SIDD263 pKa = 3.68EE264 pKa = 4.05SHH266 pKa = 5.88MTNN269 pKa = 3.42

Molecular weight: 30.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5TRJ9|Q5TRJ9_ANOGA AGAP005580-PA OS=Anopheles gambiae OX=7165 GN=3290003 PE=4 SV=3
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.12WRR6 pKa = 11.84KK7 pKa = 9.1KK8 pKa = 9.32RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 7.85RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.6MRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 11.11

Molecular weight: 3.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15553

1546

17099

9740585

19

16070

569.7

63.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.641 ± 0.021

1.832 ± 0.017

5.282 ± 0.015

6.365 ± 0.03

3.445 ± 0.017

6.717 ± 0.028

2.703 ± 0.012

4.814 ± 0.015

5.256 ± 0.019

8.898 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.26 ± 0.01

4.415 ± 0.015

5.479 ± 0.023

4.888 ± 0.026

5.618 ± 0.018

8.122 ± 0.031

6.032 ± 0.022

6.243 ± 0.016

0.968 ± 0.006

3.019 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski