Zymoseptoria tritici (strain CBS 115943 / IPO323) (Speckled leaf blotch fungus) (Septoria tritici)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Zymoseptoria; Zymoseptoria tritici

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10972 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F9X1J0|F9X1J0_ZYMTI Uncharacterized protein OS=Zymoseptoria tritici (strain CBS 115943 / IPO323) OX=336722 GN=MYCGRDRAFT_67504 PE=4 SV=1
MM1 pKa = 7.27QPTSMLLTLLGLSTYY16 pKa = 9.78TSASSLQINNYY27 pKa = 8.27CTDD30 pKa = 3.97SLWTTLTSPSQPFAEE45 pKa = 4.56PFEE48 pKa = 4.71IPSGVANISDD58 pKa = 3.31ISGSGNSLGVTFSKK72 pKa = 10.6DD73 pKa = 3.26YY74 pKa = 10.56YY75 pKa = 11.38AEE77 pKa = 4.11NTPKK81 pKa = 10.54LVLGFTSVNGRR92 pKa = 11.84LWWSLGDD99 pKa = 3.66ISGDD103 pKa = 3.58PFKK106 pKa = 11.42GKK108 pKa = 10.19AFNVTTPDD116 pKa = 3.25HH117 pKa = 7.0DD118 pKa = 4.35GKK120 pKa = 10.57DD121 pKa = 3.61VCGDD125 pKa = 3.32AVGVDD130 pKa = 4.16GEE132 pKa = 4.46NHH134 pKa = 6.13NCDD137 pKa = 3.65EE138 pKa = 4.78GDD140 pKa = 3.45FTLTFNPCFEE150 pKa = 4.35

Molecular weight: 16.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F9XK48|F9XK48_ZYMTI Uncharacterized protein OS=Zymoseptoria tritici (strain CBS 115943 / IPO323) OX=336722 GN=MYCGRDRAFT_105787 PE=4 SV=1
MM1 pKa = 7.25LRR3 pKa = 11.84VRR5 pKa = 11.84TRR7 pKa = 11.84SRR9 pKa = 11.84NLWRR13 pKa = 11.84NTMLATRR20 pKa = 11.84RR21 pKa = 11.84TQLRR25 pKa = 11.84ISRR28 pKa = 11.84AMRR31 pKa = 11.84LLGSHH36 pKa = 6.25NSSSRR41 pKa = 11.84RR42 pKa = 11.84LSKK45 pKa = 11.02AMAVMTSS52 pKa = 2.96

Molecular weight: 6.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10972

0

10972

4798142

48

4613

437.3

48.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.075 ± 0.019

1.267 ± 0.009

5.808 ± 0.019

6.28 ± 0.027

3.632 ± 0.017

7.017 ± 0.024

2.44 ± 0.01

4.669 ± 0.018

4.897 ± 0.026

8.778 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.248 ± 0.008

3.569 ± 0.014

5.813 ± 0.022

4.025 ± 0.017

6.186 ± 0.021

7.948 ± 0.035

6.074 ± 0.019

6.14 ± 0.016

1.457 ± 0.009

2.678 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski