Bacillaceae bacterium SAS-127

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; unclassified Bacillaceae

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3527 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A261QHB9|A0A261QHB9_9BACI tRNA pseudouridine synthase B OS=Bacillaceae bacterium SAS-127 OX=2015203 GN=truB PE=3 SV=1
MM1 pKa = 7.42LVEE4 pKa = 4.43EE5 pKa = 4.58NNMVSIWIGRR15 pKa = 11.84AEE17 pKa = 4.58DD18 pKa = 3.64KK19 pKa = 11.14EE20 pKa = 4.79KK21 pKa = 10.41IDD23 pKa = 4.0SLMEE27 pKa = 3.64YY28 pKa = 10.22AYY30 pKa = 10.67DD31 pKa = 3.89EE32 pKa = 5.85DD33 pKa = 6.16GDD35 pKa = 3.91ATPPKK40 pKa = 9.87FVQASEE46 pKa = 4.08IDD48 pKa = 4.15DD49 pKa = 3.87IYY51 pKa = 10.81IDD53 pKa = 3.69EE54 pKa = 5.16DD55 pKa = 3.69FVEE58 pKa = 5.02VSFQEE63 pKa = 4.41STDD66 pKa = 3.54SLTSLLEE73 pKa = 3.86GHH75 pKa = 6.33SYY77 pKa = 10.1SHH79 pKa = 6.49YY80 pKa = 10.5FLPEE84 pKa = 4.01LFKK87 pKa = 11.3HH88 pKa = 6.72FDD90 pKa = 3.54DD91 pKa = 4.52QLEE94 pKa = 4.34EE95 pKa = 4.2NFNVTLLIYY104 pKa = 10.53HH105 pKa = 6.32FEE107 pKa = 4.23FNGAVSEE114 pKa = 4.41VAFDD118 pKa = 4.0GVQMFYY124 pKa = 11.11VGALPFEE131 pKa = 5.09KK132 pKa = 10.03II133 pKa = 3.53

Molecular weight: 15.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A261QI43|A0A261QI43_9BACI IDEAL domain-containing protein OS=Bacillaceae bacterium SAS-127 OX=2015203 GN=CEW92_06020 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.21RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84SKK14 pKa = 9.59VHH16 pKa = 5.83GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSKK25 pKa = 10.18NGRR28 pKa = 11.84RR29 pKa = 11.84VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3527

0

3527

1042885

25

1860

295.7

33.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.305 ± 0.049

0.752 ± 0.013

4.837 ± 0.031

7.737 ± 0.048

4.591 ± 0.035

6.616 ± 0.04

2.178 ± 0.02

7.515 ± 0.042

6.795 ± 0.034

9.892 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.965 ± 0.021

3.988 ± 0.025

3.553 ± 0.024

4.275 ± 0.03

4.029 ± 0.029

5.893 ± 0.033

5.423 ± 0.027

7.201 ± 0.029

1.037 ± 0.015

3.419 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski