Helicobacter sp. 10-6591

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Helicobacteraceae; Helicobacter; unclassified Helicobacter

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1547 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A329ZR07|A0A329ZR07_9HELI Phospho-sugar mutase OS=Helicobacter sp. 10-6591 OX=2004998 GN=CCY97_00535 PE=3 SV=1
MM1 pKa = 7.94ALMISDD7 pKa = 3.87EE8 pKa = 4.99CIACDD13 pKa = 3.69ACRR16 pKa = 11.84EE17 pKa = 4.13EE18 pKa = 4.9CPNDD22 pKa = 4.48AIEE25 pKa = 5.07ADD27 pKa = 3.77DD28 pKa = 4.3PFYY31 pKa = 11.43VIDD34 pKa = 5.24PDD36 pKa = 4.18LCTEE40 pKa = 4.39CVGCYY45 pKa = 9.94DD46 pKa = 4.15EE47 pKa = 5.12PNCIVVCPVDD57 pKa = 5.76AIVLDD62 pKa = 4.06PDD64 pKa = 3.43NVEE67 pKa = 3.91NAEE70 pKa = 3.92EE71 pKa = 3.76LRR73 pKa = 11.84YY74 pKa = 10.23KK75 pKa = 10.66FEE77 pKa = 3.92QLQGQEE83 pKa = 3.91

Molecular weight: 9.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A329ZJC6|A0A329ZJC6_9HELI 50S ribosomal protein L32 OS=Helicobacter sp. 10-6591 OX=2004998 GN=rpmF PE=3 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.04QPHH8 pKa = 4.86NTPRR12 pKa = 11.84KK13 pKa = 7.34RR14 pKa = 11.84THH16 pKa = 6.22GFRR19 pKa = 11.84TRR21 pKa = 11.84MKK23 pKa = 8.66TKK25 pKa = 8.69TGRR28 pKa = 11.84RR29 pKa = 11.84IINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.51GRR39 pKa = 11.84KK40 pKa = 8.68RR41 pKa = 11.84LSVV44 pKa = 3.2

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1547

0

1547

492504

37

2885

318.4

35.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.814 ± 0.05

1.37 ± 0.024

5.083 ± 0.042

6.125 ± 0.058

5.319 ± 0.066

6.116 ± 0.067

2.035 ± 0.025

8.208 ± 0.048

7.54 ± 0.058

10.709 ± 0.082

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.124 ± 0.026

5.351 ± 0.069

3.148 ± 0.036

4.137 ± 0.044

3.9 ± 0.036

7.235 ± 0.045

4.784 ± 0.052

5.632 ± 0.053

0.708 ± 0.018

3.662 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski