Janibacter sp. HTCC2649

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Intrasporangiaceae; Janibacter; unclassified Janibacter

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4081 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A3TLQ1|A3TLQ1_9MICO Carbamoyl-phosphate synthase small chain OS=Janibacter sp. HTCC2649 OX=313589 GN=carA PE=3 SV=1
MM1 pKa = 7.75LSRR4 pKa = 11.84WCALTGIGVVVAAMGLTPSAAAAPYY29 pKa = 10.0AAVASVSPGVQYY41 pKa = 11.09LGDD44 pKa = 3.8STGVTFTFMVANTGTADD61 pKa = 3.43SIGAVEE67 pKa = 4.33IDD69 pKa = 3.53RR70 pKa = 11.84PGKK73 pKa = 8.52QWQITGCPSAPTGWSAQRR91 pKa = 11.84SDD93 pKa = 3.32TACRR97 pKa = 11.84FRR99 pKa = 11.84SAAGTGDD106 pKa = 4.79DD107 pKa = 4.45IAPGQQTTAFRR118 pKa = 11.84VTAVVAPGTQNLTGTWPVKK137 pKa = 10.43VSRR140 pKa = 11.84SSNFDD145 pKa = 3.34TKK147 pKa = 11.25SKK149 pKa = 10.71LIAAASTSPGLGITAHH165 pKa = 6.48SFQILDD171 pKa = 3.69AVVDD175 pKa = 4.08PATSTVGSACPSPAKK190 pKa = 10.45SAVTGSAGHH199 pKa = 6.56TIVICGRR206 pKa = 11.84NRR208 pKa = 11.84TTGTLTPTAAQSSLGGTFLAGQGAFSSGPVAPTTASVVLGRR249 pKa = 11.84WSNVTITTLAGPGKK263 pKa = 8.44TVIAKK268 pKa = 10.0IGSATNRR275 pKa = 11.84TSPLTTLTGYY285 pKa = 7.46TALNTPPNASDD296 pKa = 3.63DD297 pKa = 3.78AVTIDD302 pKa = 4.39EE303 pKa = 5.0DD304 pKa = 4.33SSTSFDD310 pKa = 3.55PRR312 pKa = 11.84TNDD315 pKa = 3.52SDD317 pKa = 4.31PDD319 pKa = 3.55GDD321 pKa = 4.39AFTISAIGTTGTQGAVTITGGGTGLTYY348 pKa = 10.95DD349 pKa = 4.34PDD351 pKa = 3.98GKK353 pKa = 10.6FDD355 pKa = 3.7NLAPGEE361 pKa = 4.08QDD363 pKa = 2.6TDD365 pKa = 3.5TFTYY369 pKa = 9.96TITDD373 pKa = 3.33AFGGSDD379 pKa = 3.34SATVTVTVTGLADD392 pKa = 3.56PPVAVNDD399 pKa = 3.87SKK401 pKa = 10.21TVVEE405 pKa = 4.84DD406 pKa = 4.29APATTVDD413 pKa = 3.53VLANDD418 pKa = 3.87TDD420 pKa = 3.88VDD422 pKa = 4.1GGPMSIASVTQPANGTVVITGGGTGLTYY450 pKa = 10.64EE451 pKa = 4.81PDD453 pKa = 3.14AGYY456 pKa = 10.39CNSVAGPADD465 pKa = 3.73PFTYY469 pKa = 10.04TLAPGGSSATVSVTVTCVDD488 pKa = 4.21DD489 pKa = 5.03LPVAVDD495 pKa = 3.55DD496 pKa = 4.7AKK498 pKa = 10.56TVVEE502 pKa = 4.71DD503 pKa = 3.93AAATAVDD510 pKa = 3.93VLANDD515 pKa = 3.87TDD517 pKa = 3.85VDD519 pKa = 3.88GGPISIASVTQPANGTVVITGGGTGLTYY547 pKa = 10.67QPDD550 pKa = 3.23ASYY553 pKa = 11.5CNNPPGTTPDD563 pKa = 3.27TFTYY567 pKa = 9.99TLTPGGSTAIVSVTVTCVDD586 pKa = 4.21DD587 pKa = 5.03LPVAVDD593 pKa = 3.59DD594 pKa = 5.01AKK596 pKa = 10.7TLVEE600 pKa = 4.54DD601 pKa = 4.36AAATAVDD608 pKa = 3.85VLVNDD613 pKa = 4.04TDD615 pKa = 3.66VDD617 pKa = 3.79GGPISITSVTQPTNGTVVITGGGTGLTYY645 pKa = 10.68QPDD648 pKa = 3.6ANYY651 pKa = 10.66CNNPPGTTLDD661 pKa = 3.42TFTYY665 pKa = 8.71TLSPGGSTATVSLTVTCVNDD685 pKa = 3.61PPNAVDD691 pKa = 3.58DD692 pKa = 4.58AKK694 pKa = 10.54TVVEE698 pKa = 4.71DD699 pKa = 3.93AAATAVDD706 pKa = 3.93VLANDD711 pKa = 4.13TDD713 pKa = 4.37PDD715 pKa = 4.13GDD717 pKa = 4.6PISITSVTQPANGTVAITGGGTGLTYY743 pKa = 10.71DD744 pKa = 4.41PNANYY749 pKa = 10.63CNDD752 pKa = 4.12PPGSTPDD759 pKa = 3.22TFTYY763 pKa = 9.12TVNGGSTGTVSVMVTCVDD781 pKa = 4.17DD782 pKa = 5.0LPVAVDD788 pKa = 4.07DD789 pKa = 4.85SEE791 pKa = 4.83QVTLNATATALMVLANDD808 pKa = 3.74TDD810 pKa = 3.73VDD812 pKa = 4.01GGALSIASVTQPTNGTVVITGGGTGLTYY840 pKa = 10.64EE841 pKa = 4.82PDD843 pKa = 3.11AGYY846 pKa = 10.96CDD848 pKa = 5.36DD849 pKa = 5.29PPGTTPDD856 pKa = 3.27TFTYY860 pKa = 9.17TLNGGSTAMVSVRR873 pKa = 11.84VVCDD877 pKa = 4.05DD878 pKa = 4.06PPVAVDD884 pKa = 3.52DD885 pKa = 4.57AKK887 pKa = 10.56TVVEE891 pKa = 4.71DD892 pKa = 3.93AAATAVDD899 pKa = 3.93VLANDD904 pKa = 3.87TDD906 pKa = 3.85VDD908 pKa = 3.88GGPISIASATQPTNGTVVITGGGTGLTYY936 pKa = 10.67QPDD939 pKa = 3.23ASYY942 pKa = 11.5CNNPPGTTLDD952 pKa = 3.47TFTYY956 pKa = 9.33TLTPGGSTATVSVTVTCVDD975 pKa = 3.83NLPVAVDD982 pKa = 3.58DD983 pKa = 4.63AKK985 pKa = 10.56TVVEE989 pKa = 4.71DD990 pKa = 3.93AAATAVDD997 pKa = 3.93VLANDD1002 pKa = 3.87TDD1004 pKa = 3.85VDD1006 pKa = 3.88GGPISIASVNQPTNGTVVISGGGTGLTYY1034 pKa = 10.68QPDD1037 pKa = 3.6ANYY1040 pKa = 10.66CNNPPGTTLDD1050 pKa = 3.47TFTYY1054 pKa = 9.33TLTPGGSTATVSLTVTCVNDD1074 pKa = 3.61PPNAVDD1080 pKa = 4.07DD1081 pKa = 4.09ATSVGEE1087 pKa = 4.36GAAGAAVPVLANDD1100 pKa = 3.94TDD1102 pKa = 4.45PDD1104 pKa = 3.95PTDD1107 pKa = 3.43VLSVTSVDD1115 pKa = 3.39TTGTIGGVSLVGGVVTYY1132 pKa = 10.87NPNGQFEE1139 pKa = 4.48YY1140 pKa = 10.65LAAGATATDD1149 pKa = 3.72TFSYY1153 pKa = 9.86TVSDD1157 pKa = 4.24GNGGTDD1163 pKa = 3.28TAVVTVTVTGANDD1176 pKa = 3.62PLVAVNDD1183 pKa = 3.94TGTTDD1188 pKa = 3.3EE1189 pKa = 5.06DD1190 pKa = 4.14TTLSQSAPGVLANDD1204 pKa = 4.02TDD1206 pKa = 4.75PDD1208 pKa = 3.85TGDD1211 pKa = 3.07TRR1213 pKa = 11.84TVTRR1217 pKa = 11.84LNGSATLTGTSTSGAAVSIAANGSFTYY1244 pKa = 10.7NPGGLFQGLSTGQSATDD1261 pKa = 3.41TFTYY1265 pKa = 9.08TAQDD1269 pKa = 3.29GSGAQSTATVTITINGVSDD1288 pKa = 3.76APTAVTDD1295 pKa = 3.81SFQTHH1300 pKa = 6.31GNTNLFVGTSRR1311 pKa = 11.84PVGEE1315 pKa = 4.4AGKK1318 pKa = 10.44VLTGSVLTNDD1328 pKa = 4.13TDD1330 pKa = 4.56PDD1332 pKa = 3.86TAQANLVAEE1341 pKa = 4.58PVTNAPTTLGGSITIEE1357 pKa = 3.61SDD1359 pKa = 3.62GNFTFYY1365 pKa = 10.47PDD1367 pKa = 4.81DD1368 pKa = 4.9GDD1370 pKa = 4.49SGVTDD1375 pKa = 3.41SFTYY1379 pKa = 9.9RR1380 pKa = 11.84VCDD1383 pKa = 4.02ASPCNASTVANATGTLNLPISSSQVWYY1410 pKa = 10.49VRR1412 pKa = 11.84NNAVAGGDD1420 pKa = 3.9GTSEE1424 pKa = 4.46APFDD1428 pKa = 3.93TLGEE1432 pKa = 4.27AEE1434 pKa = 4.25TAADD1438 pKa = 4.6AGDD1441 pKa = 3.52TTYY1444 pKa = 11.17VFDD1447 pKa = 5.75GDD1449 pKa = 3.88NTSLNLGSGFVMAAGEE1465 pKa = 4.19RR1466 pKa = 11.84LLGEE1470 pKa = 4.26AVTLQVGADD1479 pKa = 3.47VLYY1482 pKa = 10.47TGVPANRR1489 pKa = 11.84PTLTANDD1496 pKa = 3.84EE1497 pKa = 4.45DD1498 pKa = 4.9VVTLANATTVRR1509 pKa = 11.84GVQLDD1514 pKa = 3.73PQGAGGGIAGIGTASGPGTSTIADD1538 pKa = 3.29VRR1540 pKa = 11.84IIDD1543 pKa = 3.72TGTAGTQPGLEE1554 pKa = 4.05LDD1556 pKa = 4.17GVEE1559 pKa = 4.12GTYY1562 pKa = 10.34DD1563 pKa = 3.38ISDD1566 pKa = 3.73LTVDD1570 pKa = 3.73NSAATGQTAGSIGVRR1585 pKa = 11.84LNRR1588 pKa = 11.84NILGFTANFASAGTISLTTKK1608 pKa = 9.6GAKK1611 pKa = 9.82ALEE1614 pKa = 4.11VTGANMGLASVFDD1627 pKa = 5.71DD1628 pKa = 3.29ITVTGSPTGGVSMVNSLGSGTAFGDD1653 pKa = 4.11GVGVDD1658 pKa = 4.18LALTTTGGSAFNLQGTGTVSVPAAGTANVNATAGAGVFVNGSSGSSLAFDD1708 pKa = 4.26DD1709 pKa = 4.0VTVVSNPSGVGIAAVALGTGSFSAATGSISNTAGTAFTTGTGNGPISYY1757 pKa = 10.36GGTINNGSGRR1767 pKa = 11.84SVEE1770 pKa = 4.13VSARR1774 pKa = 11.84TGGTVTLTGNINDD1787 pKa = 4.15TNDD1790 pKa = 2.99VGGGIFVTGNSGGSTVLSGTTKK1812 pKa = 10.05TLNTGSEE1819 pKa = 4.2KK1820 pKa = 10.85AVDD1823 pKa = 4.58FSSSDD1828 pKa = 3.06GHH1830 pKa = 6.03TLTLSGGGLDD1840 pKa = 3.52IDD1842 pKa = 4.38TVSGTGVDD1850 pKa = 3.59AASSGTLNITGAGNTIDD1867 pKa = 3.64TSAGKK1872 pKa = 10.02ALSVSDD1878 pKa = 3.89TDD1880 pKa = 3.88VGPSPLSLQRR1890 pKa = 11.84ISSLGGAGITLSNTGSNNALVVASSGSGTCTAADD1924 pKa = 3.35QSGCTGGEE1932 pKa = 3.62IRR1934 pKa = 11.84SATGVDD1940 pKa = 3.81DD1941 pKa = 5.41SGALPTGTGIVLNNTRR1957 pKa = 11.84GVSLTRR1963 pKa = 11.84MHH1965 pKa = 6.8LHH1967 pKa = 6.38DD1968 pKa = 4.6HH1969 pKa = 6.11SNYY1972 pKa = 9.72GIRR1975 pKa = 11.84GTSVVGFSMVDD1986 pKa = 3.26SVVNGVNGTNGTTTFKK2002 pKa = 10.73DD2003 pKa = 3.62GSVRR2007 pKa = 11.84FVEE2010 pKa = 4.34LTGTVAMTNVALSGGYY2026 pKa = 7.55FTNLMVDD2033 pKa = 3.55NTAGILNGTFDD2044 pKa = 4.19NVDD2047 pKa = 3.37SGTINATGGDD2057 pKa = 3.66DD2058 pKa = 3.5AVQFEE2063 pKa = 5.29GIGTSTMNVDD2073 pKa = 4.1YY2074 pKa = 10.45KK2075 pKa = 11.32NSAIGTATGDD2085 pKa = 3.54LFQYY2089 pKa = 10.42IGDD2092 pKa = 3.94GTGGGNLDD2100 pKa = 3.54LTSNAFTNNEE2110 pKa = 3.75PSIATGGGGVAIVAGAKK2127 pKa = 9.64GAATLDD2133 pKa = 3.29IVSNTFRR2140 pKa = 11.84DD2141 pKa = 3.76SLTNALTVIKK2151 pKa = 10.57SADD2154 pKa = 3.3ATAGTNPLVSNITGNTIGVAATANSGSFEE2183 pKa = 4.23GDD2185 pKa = 2.72GMEE2188 pKa = 3.91ITTFGDD2194 pKa = 3.54GNATFNVLNNNISQYY2209 pKa = 10.39NSSGIQFVAGSGVTATGQMNLNVSGNVVGNPGTNPLITLLQGIRR2253 pKa = 11.84VDD2255 pKa = 4.04SGVDD2259 pKa = 3.15TADD2262 pKa = 3.41TFSTCVKK2269 pKa = 10.2FGANSISGAGGSAGRR2284 pKa = 11.84DD2285 pKa = 3.59FRR2287 pKa = 11.84LVASQQTTLRR2297 pKa = 11.84QPGYY2301 pKa = 10.82VGGSTDD2307 pKa = 2.99GTAFANYY2314 pKa = 8.74AASLIGSGAAGTAVANAPGTFSGAGSTCPP2343 pKa = 3.86

Molecular weight: 230.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A3THR1|A3THR1_9MICO Putative transcriptional regulator OS=Janibacter sp. HTCC2649 OX=313589 GN=JNB_09369 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4081

0

4081

1293981

18

3687

317.1

33.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.954 ± 0.052

0.658 ± 0.008

6.333 ± 0.035

5.438 ± 0.04

2.834 ± 0.026

9.291 ± 0.041

2.221 ± 0.022

3.995 ± 0.028

2.312 ± 0.029

9.919 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.892 ± 0.016

1.979 ± 0.025

5.459 ± 0.029

2.8 ± 0.018

7.035 ± 0.045

5.782 ± 0.026

6.556 ± 0.04

9.156 ± 0.037

1.573 ± 0.017

1.814 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski