Amino acid dipepetide frequency for Halanaerobium saccharolyticum subsp. saccharolyticum DSM 6643

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.797AlaAla: 7.797 ± 0.133
0.465AlaCys: 0.465 ± 0.025
4.312AlaAsp: 4.312 ± 0.079
7.362AlaGlu: 7.362 ± 0.109
2.812AlaPhe: 2.812 ± 0.068
5.753AlaGly: 5.753 ± 0.092
1.046AlaHis: 1.046 ± 0.041
4.755AlaIle: 4.755 ± 0.095
4.755AlaLys: 4.755 ± 0.074
6.717AlaLeu: 6.717 ± 0.105
1.684AlaMet: 1.684 ± 0.05
2.75AlaAsn: 2.75 ± 0.063
1.671AlaPro: 1.671 ± 0.045
2.166AlaGln: 2.166 ± 0.052
2.586AlaArg: 2.586 ± 0.056
3.681AlaSer: 3.681 ± 0.074
2.341AlaThr: 2.341 ± 0.06
6.083AlaVal: 6.083 ± 0.097
0.447AlaTrp: 0.447 ± 0.026
2.153AlaTyr: 2.153 ± 0.051
0.0AlaXaa: 0.0 ± 0.0
Cys
0.348CysAla: 0.348 ± 0.019
0.082CysCys: 0.082 ± 0.01
0.39CysAsp: 0.39 ± 0.025
0.388CysGlu: 0.388 ± 0.022
0.198CysPhe: 0.198 ± 0.017
0.663CysGly: 0.663 ± 0.033
0.153CysHis: 0.153 ± 0.013
0.414CysIle: 0.414 ± 0.024
0.354CysLys: 0.354 ± 0.024
0.497CysLeu: 0.497 ± 0.024
0.124CysMet: 0.124 ± 0.013
0.302CysAsn: 0.302 ± 0.02
0.398CysPro: 0.398 ± 0.023
0.216CysGln: 0.216 ± 0.015
0.291CysArg: 0.291 ± 0.019
0.474CysSer: 0.474 ± 0.025
0.281CysThr: 0.281 ± 0.019
0.328CysVal: 0.328 ± 0.023
0.051CysTrp: 0.051 ± 0.008
0.207CysTyr: 0.207 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
2.956AspAla: 2.956 ± 0.061
0.403AspCys: 0.403 ± 0.024
2.994AspAsp: 2.994 ± 0.086
4.028AspGlu: 4.028 ± 0.089
3.424AspPhe: 3.424 ± 0.064
3.147AspGly: 3.147 ± 0.076
0.961AspHis: 0.961 ± 0.033
5.197AspIle: 5.197 ± 0.085
4.664AspLys: 4.664 ± 0.08
6.766AspLeu: 6.766 ± 0.081
1.185AspMet: 1.185 ± 0.032
3.228AspAsn: 3.228 ± 0.071
1.817AspPro: 1.817 ± 0.051
2.717AspGln: 2.717 ± 0.064
2.151AspArg: 2.151 ± 0.06
3.319AspSer: 3.319 ± 0.064
2.036AspThr: 2.036 ± 0.054
3.187AspVal: 3.187 ± 0.064
0.521AspTrp: 0.521 ± 0.026
3.06AspTyr: 3.06 ± 0.069
0.0AspXaa: 0.0 ± 0.0
Glu
5.945GluAla: 5.945 ± 0.09
0.352GluCys: 0.352 ± 0.023
4.505GluAsp: 4.505 ± 0.091
7.947GluGlu: 7.947 ± 0.129
3.305GluPhe: 3.305 ± 0.069
3.942GluGly: 3.942 ± 0.071
1.054GluHis: 1.054 ± 0.038
8.96GluIle: 8.96 ± 0.114
8.45GluLys: 8.45 ± 0.128
8.652GluLeu: 8.652 ± 0.108
2.455GluMet: 2.455 ± 0.055
5.681GluAsn: 5.681 ± 0.1
1.611GluPro: 1.611 ± 0.058
2.009GluGln: 2.009 ± 0.048
2.785GluArg: 2.785 ± 0.054
3.988GluSer: 3.988 ± 0.08
3.059GluThr: 3.059 ± 0.061
5.239GluVal: 5.239 ± 0.079
0.489GluTrp: 0.489 ± 0.024
2.833GluTyr: 2.833 ± 0.055
0.0GluXaa: 0.0 ± 0.0
Phe
3.091PheAla: 3.091 ± 0.076
0.28PheCys: 0.28 ± 0.02
2.668PheAsp: 2.668 ± 0.062
3.019PheGlu: 3.019 ± 0.068
2.276PhePhe: 2.276 ± 0.06
2.906PheGly: 2.906 ± 0.075
0.592PheHis: 0.592 ± 0.027
4.114PheIle: 4.114 ± 0.094
3.662PheLys: 3.662 ± 0.077
4.538PheLeu: 4.538 ± 0.101
1.075PheMet: 1.075 ± 0.036
2.824PheAsn: 2.824 ± 0.055
1.359PhePro: 1.359 ± 0.04
1.047PheGln: 1.047 ± 0.034
1.337PheArg: 1.337 ± 0.037
3.494PheSer: 3.494 ± 0.073
2.111PheThr: 2.111 ± 0.051
2.446PheVal: 2.446 ± 0.052
0.361PheTrp: 0.361 ± 0.021
1.736PheTyr: 1.736 ± 0.052
0.0PheXaa: 0.0 ± 0.0
Gly
4.603GlyAla: 4.603 ± 0.083
0.55GlyCys: 0.55 ± 0.026
3.435GlyAsp: 3.435 ± 0.065
4.962GlyGlu: 4.962 ± 0.082
2.927GlyPhe: 2.927 ± 0.07
4.789GlyGly: 4.789 ± 0.097
1.104GlyHis: 1.104 ± 0.039
6.023GlyIle: 6.023 ± 0.096
4.426GlyLys: 4.426 ± 0.079
6.093GlyLeu: 6.093 ± 0.099
1.753GlyMet: 1.753 ± 0.05
2.59GlyAsn: 2.59 ± 0.063
1.619GlyPro: 1.619 ± 0.044
1.771GlyGln: 1.771 ± 0.047
2.536GlyArg: 2.536 ± 0.058
4.047GlySer: 4.047 ± 0.079
3.13GlyThr: 3.13 ± 0.057
4.549GlyVal: 4.549 ± 0.078
0.578GlyTrp: 0.578 ± 0.028
2.569GlyTyr: 2.569 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
0.829HisAla: 0.829 ± 0.034
0.152HisCys: 0.152 ± 0.012
0.741HisAsp: 0.741 ± 0.03
0.778HisGlu: 0.778 ± 0.034
0.77HisPhe: 0.77 ± 0.032
1.028HisGly: 1.028 ± 0.038
0.38HisHis: 0.38 ± 0.021
1.12HisIle: 1.12 ± 0.041
0.943HisLys: 0.943 ± 0.036
1.634HisLeu: 1.634 ± 0.044
0.258HisMet: 0.258 ± 0.016
0.851HisAsn: 0.851 ± 0.039
0.809HisPro: 0.809 ± 0.032
0.707HisGln: 0.707 ± 0.026
0.616HisArg: 0.616 ± 0.026
0.951HisSer: 0.951 ± 0.035
0.578HisThr: 0.578 ± 0.026
0.664HisVal: 0.664 ± 0.028
0.125HisTrp: 0.125 ± 0.013
0.659HisTyr: 0.659 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
6.283IleAla: 6.283 ± 0.109
0.555IleCys: 0.555 ± 0.028
5.482IleAsp: 5.482 ± 0.082
7.131IleGlu: 7.131 ± 0.102
4.316IlePhe: 4.316 ± 0.101
5.368IleGly: 5.368 ± 0.096
1.006IleHis: 1.006 ± 0.035
9.032IleIle: 9.032 ± 0.14
7.64IleLys: 7.64 ± 0.096
8.31IleLeu: 8.31 ± 0.119
2.108IleMet: 2.108 ± 0.054
5.395IleAsn: 5.395 ± 0.108
3.205IlePro: 3.205 ± 0.069
1.826IleGln: 1.826 ± 0.046
2.886IleArg: 2.886 ± 0.054
6.227IleSer: 6.227 ± 0.096
4.381IleThr: 4.381 ± 0.066
4.887IleVal: 4.887 ± 0.077
0.558IleTrp: 0.558 ± 0.029
3.273IleTyr: 3.273 ± 0.068
0.0IleXaa: 0.0 ± 0.0
Lys
5.421LysAla: 5.421 ± 0.089
0.362LysCys: 0.362 ± 0.023
4.732LysAsp: 4.732 ± 0.086
8.679LysGlu: 8.679 ± 0.123
2.801LysPhe: 2.801 ± 0.059
3.973LysGly: 3.973 ± 0.072
0.954LysHis: 0.954 ± 0.031
8.32LysIle: 8.32 ± 0.129
8.584LysLys: 8.584 ± 0.138
7.711LysLeu: 7.711 ± 0.105
2.306LysMet: 2.306 ± 0.054
5.996LysAsn: 5.996 ± 0.106
1.682LysPro: 1.682 ± 0.043
1.97LysGln: 1.97 ± 0.051
2.895LysArg: 2.895 ± 0.073
4.305LysSer: 4.305 ± 0.072
3.663LysThr: 3.663 ± 0.064
4.699LysVal: 4.699 ± 0.078
0.499LysTrp: 0.499 ± 0.028
3.31LysTyr: 3.31 ± 0.066
0.0LysXaa: 0.0 ± 0.0
Leu
7.64LeuAla: 7.64 ± 0.109
0.416LeuCys: 0.416 ± 0.021
5.855LeuAsp: 5.855 ± 0.085
8.307LeuGlu: 8.307 ± 0.113
4.128LeuPhe: 4.128 ± 0.093
6.144LeuGly: 6.144 ± 0.109
1.216LeuHis: 1.216 ± 0.041
8.929LeuIle: 8.929 ± 0.127
9.641LeuLys: 9.641 ± 0.14
9.064LeuLeu: 9.064 ± 0.148
2.253LeuMet: 2.253 ± 0.045
6.305LeuAsn: 6.305 ± 0.104
3.388LeuPro: 3.388 ± 0.063
2.458LeuGln: 2.458 ± 0.055
3.329LeuArg: 3.329 ± 0.069
6.682LeuSer: 6.682 ± 0.1
4.624LeuThr: 4.624 ± 0.088
5.4LeuVal: 5.4 ± 0.084
0.592LeuTrp: 0.592 ± 0.026
2.882LeuTyr: 2.882 ± 0.061
0.0LeuXaa: 0.0 ± 0.0
Met
2.065MetAla: 2.065 ± 0.053
0.085MetCys: 0.085 ± 0.01
1.418MetAsp: 1.418 ± 0.042
1.842MetGlu: 1.842 ± 0.051
0.862MetPhe: 0.862 ± 0.039
1.811MetGly: 1.811 ± 0.05
0.345MetHis: 0.345 ± 0.022
2.101MetIle: 2.101 ± 0.053
1.927MetLys: 1.927 ± 0.046
2.217MetLeu: 2.217 ± 0.068
0.645MetMet: 0.645 ± 0.028
1.251MetAsn: 1.251 ± 0.037
0.902MetPro: 0.902 ± 0.03
0.853MetGln: 0.853 ± 0.031
0.927MetArg: 0.927 ± 0.037
1.463MetSer: 1.463 ± 0.039
1.27MetThr: 1.27 ± 0.039
1.5MetVal: 1.5 ± 0.046
0.14MetTrp: 0.14 ± 0.013
0.539MetTyr: 0.539 ± 0.024
0.0MetXaa: 0.0 ± 0.0
Asn
2.574AsnAla: 2.574 ± 0.066
0.404AsnCys: 0.404 ± 0.023
3.077AsnAsp: 3.077 ± 0.067
3.681AsnGlu: 3.681 ± 0.071
2.854AsnPhe: 2.854 ± 0.064
2.86AsnGly: 2.86 ± 0.07
0.842AsnHis: 0.842 ± 0.034
5.349AsnIle: 5.349 ± 0.102
5.278AsnLys: 5.278 ± 0.091
6.375AsnLeu: 6.375 ± 0.101
1.315AsnMet: 1.315 ± 0.04
4.219AsnAsn: 4.219 ± 0.102
2.201AsnPro: 2.201 ± 0.055
2.31AsnGln: 2.31 ± 0.051
2.006AsnArg: 2.006 ± 0.054
3.522AsnSer: 3.522 ± 0.088
2.218AsnThr: 2.218 ± 0.049
2.635AsnVal: 2.635 ± 0.055
0.542AsnTrp: 0.542 ± 0.029
2.958AsnTyr: 2.958 ± 0.074
0.0AsnXaa: 0.0 ± 0.0
Pro
2.404ProAla: 2.404 ± 0.049
0.205ProCys: 0.205 ± 0.016
1.915ProAsp: 1.915 ± 0.05
3.257ProGlu: 3.257 ± 0.07
1.442ProPhe: 1.442 ± 0.041
2.364ProGly: 2.364 ± 0.059
0.579ProHis: 0.579 ± 0.029
2.296ProIle: 2.296 ± 0.054
1.697ProLys: 1.697 ± 0.04
2.91ProLeu: 2.91 ± 0.066
0.658ProMet: 0.658 ± 0.029
1.186ProAsn: 1.186 ± 0.037
0.855ProPro: 0.855 ± 0.031
0.943ProGln: 0.943 ± 0.032
0.925ProArg: 0.925 ± 0.03
1.479ProSer: 1.479 ± 0.039
1.416ProThr: 1.416 ± 0.043
2.32ProVal: 2.32 ± 0.059
0.283ProTrp: 0.283 ± 0.022
1.227ProTyr: 1.227 ± 0.035
0.0ProXaa: 0.0 ± 0.0
Gln
2.266GlnAla: 2.266 ± 0.064
0.145GlnCys: 0.145 ± 0.015
1.609GlnAsp: 1.609 ± 0.045
2.558GlnGlu: 2.558 ± 0.064
1.112GlnPhe: 1.112 ± 0.037
1.877GlnGly: 1.877 ± 0.051
0.431GlnHis: 0.431 ± 0.024
2.74GlnIle: 2.74 ± 0.061
2.906GlnLys: 2.906 ± 0.061
3.018GlnLeu: 3.018 ± 0.066
0.694GlnMet: 0.694 ± 0.029
1.935GlnAsn: 1.935 ± 0.05
0.83GlnPro: 0.83 ± 0.033
1.309GlnGln: 1.309 ± 0.047
1.252GlnArg: 1.252 ± 0.042
1.674GlnSer: 1.674 ± 0.042
1.245GlnThr: 1.245 ± 0.044
1.663GlnVal: 1.663 ± 0.044
0.196GlnTrp: 0.196 ± 0.015
0.916GlnTyr: 0.916 ± 0.039
0.0GlnXaa: 0.0 ± 0.0
Arg
2.412ArgAla: 2.412 ± 0.053
0.241ArgCys: 0.241 ± 0.017
2.166ArgAsp: 2.166 ± 0.052
3.2ArgGlu: 3.2 ± 0.058
1.584ArgPhe: 1.584 ± 0.045
2.288ArgGly: 2.288 ± 0.051
0.541ArgHis: 0.541 ± 0.022
2.888ArgIle: 2.888 ± 0.063
2.992ArgLys: 2.992 ± 0.073
3.372ArgLeu: 3.372 ± 0.063
0.855ArgMet: 0.855 ± 0.033
1.864ArgAsn: 1.864 ± 0.049
1.05ArgPro: 1.05 ± 0.038
1.092ArgGln: 1.092 ± 0.039
1.498ArgArg: 1.498 ± 0.041
2.035ArgSer: 2.035 ± 0.049
1.552ArgThr: 1.552 ± 0.044
2.254ArgVal: 2.254 ± 0.051
0.286ArgTrp: 0.286 ± 0.021
1.314ArgTyr: 1.314 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
4.024SerAla: 4.024 ± 0.074
0.485SerCys: 0.485 ± 0.025
3.503SerAsp: 3.503 ± 0.07
4.667SerGlu: 4.667 ± 0.074
3.105SerPhe: 3.105 ± 0.062
4.534SerGly: 4.534 ± 0.072
0.965SerHis: 0.965 ± 0.033
4.819SerIle: 4.819 ± 0.078
4.278SerLys: 4.278 ± 0.082
6.289SerLeu: 6.289 ± 0.102
1.324SerMet: 1.324 ± 0.041
2.937SerAsn: 2.937 ± 0.073
1.768SerPro: 1.768 ± 0.046
2.031SerGln: 2.031 ± 0.05
2.458SerArg: 2.458 ± 0.055
4.025SerSer: 4.025 ± 0.076
2.665SerThr: 2.665 ± 0.054
3.4SerVal: 3.4 ± 0.071
0.59SerTrp: 0.59 ± 0.024
2.332SerTyr: 2.332 ± 0.054
0.0SerXaa: 0.0 ± 0.0
Thr
4.367ThrAla: 4.367 ± 0.085
0.215ThrCys: 0.215 ± 0.017
2.548ThrAsp: 2.548 ± 0.058
3.418ThrGlu: 3.418 ± 0.07
1.817ThrPhe: 1.817 ± 0.047
3.729ThrGly: 3.729 ± 0.072
0.742ThrHis: 0.742 ± 0.03
3.728ThrIle: 3.728 ± 0.075
2.751ThrLys: 2.751 ± 0.052
3.979ThrLeu: 3.979 ± 0.07
1.041ThrMet: 1.041 ± 0.034
1.883ThrAsn: 1.883 ± 0.044
1.625ThrPro: 1.625 ± 0.045
0.991ThrGln: 0.991 ± 0.032
1.371ThrArg: 1.371 ± 0.039
2.312ThrSer: 2.312 ± 0.057
2.259ThrThr: 2.259 ± 0.065
3.084ThrVal: 3.084 ± 0.069
0.229ThrTrp: 0.229 ± 0.015
1.237ThrTyr: 1.237 ± 0.038
0.0ThrXaa: 0.0 ± 0.0
Val
4.022ValAla: 4.022 ± 0.082
0.411ValCys: 0.411 ± 0.024
3.843ValAsp: 3.843 ± 0.081
5.336ValGlu: 5.336 ± 0.091
2.715ValPhe: 2.715 ± 0.065
4.101ValGly: 4.101 ± 0.071
0.833ValHis: 0.833 ± 0.038
5.698ValIle: 5.698 ± 0.089
4.876ValLys: 4.876 ± 0.076
5.727ValLeu: 5.727 ± 0.092
1.635ValMet: 1.635 ± 0.045
3.208ValAsn: 3.208 ± 0.065
1.949ValPro: 1.949 ± 0.054
1.308ValGln: 1.308 ± 0.036
1.926ValArg: 1.926 ± 0.052
3.692ValSer: 3.692 ± 0.072
2.579ValThr: 2.579 ± 0.061
4.137ValVal: 4.137 ± 0.073
0.387ValTrp: 0.387 ± 0.02
1.962ValTyr: 1.962 ± 0.046
0.0ValXaa: 0.0 ± 0.0
Trp
0.398TrpAla: 0.398 ± 0.027
0.037TrpCys: 0.037 ± 0.008
0.448TrpAsp: 0.448 ± 0.023
0.553TrpGlu: 0.553 ± 0.023
0.309TrpPhe: 0.309 ± 0.022
0.52TrpGly: 0.52 ± 0.028
0.149TrpHis: 0.149 ± 0.014
0.541TrpIle: 0.541 ± 0.026
0.478TrpLys: 0.478 ± 0.022
0.751TrpLeu: 0.751 ± 0.034
0.198TrpMet: 0.198 ± 0.015
0.435TrpAsn: 0.435 ± 0.023
0.308TrpPro: 0.308 ± 0.02
0.432TrpGln: 0.432 ± 0.026
0.279TrpArg: 0.279 ± 0.019
0.414TrpSer: 0.414 ± 0.023
0.34TrpThr: 0.34 ± 0.018
0.348TrpVal: 0.348 ± 0.019
0.108TrpTrp: 0.108 ± 0.011
0.246TrpTyr: 0.246 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.893TyrAla: 1.893 ± 0.051
0.315TyrCys: 0.315 ± 0.02
2.118TyrAsp: 2.118 ± 0.058
2.146TyrGlu: 2.146 ± 0.053
2.182TyrPhe: 2.182 ± 0.063
2.258TyrGly: 2.258 ± 0.05
0.7TyrHis: 0.7 ± 0.033
2.844TyrIle: 2.844 ± 0.064
2.559TyrLys: 2.559 ± 0.066
4.635TyrLeu: 4.635 ± 0.088
0.593TyrMet: 0.593 ± 0.026
2.378TyrAsn: 2.378 ± 0.057
1.315TyrPro: 1.315 ± 0.045
2.22TyrGln: 2.22 ± 0.062
1.431TyrArg: 1.431 ± 0.044
2.417TyrSer: 2.417 ± 0.053
1.576TyrThr: 1.576 ± 0.044
1.522TyrVal: 1.522 ± 0.042
0.31TyrTrp: 0.31 ± 0.023
1.739TyrTyr: 1.739 ± 0.051
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2662 proteins (861534 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski