Cryptococcus amylolentus CBS 6039

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes; Tremellales; Cryptococcaceae; Cryptococcus; Cryptococcus amylolentus

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9877 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E3H8V4|A0A1E3H8V4_9TREE Uncharacterized protein OS=Cryptococcus amylolentus CBS 6039 OX=1295533 GN=L202_08203 PE=4 SV=1
MM1 pKa = 7.46RR2 pKa = 11.84QGLFLSILPFLARR15 pKa = 11.84GVLANTCKK23 pKa = 10.56ARR25 pKa = 11.84GASSTSAADD34 pKa = 3.41AADD37 pKa = 3.94AASVDD42 pKa = 3.83GDD44 pKa = 3.92ASSSAADD51 pKa = 3.03IGGGLFASGGASTSGDD67 pKa = 2.98AAASVTSAEE76 pKa = 4.13ASVVTSAGQTAASGGASGEE95 pKa = 4.24AGDD98 pKa = 4.25TDD100 pKa = 3.32IAAAATSATLALTLAASDD118 pKa = 3.89AANATSTVSSAATRR132 pKa = 11.84GSASASSSATSSADD146 pKa = 3.39DD147 pKa = 3.76PMGTQVGTDD156 pKa = 3.62LVLGEE161 pKa = 4.53ASEE164 pKa = 4.46DD165 pKa = 3.86CKK167 pKa = 10.89CGYY170 pKa = 9.84KK171 pKa = 9.95ISSLNDD177 pKa = 2.86QYY179 pKa = 11.61FPYY182 pKa = 10.29QFSFAFSSVDD192 pKa = 3.59DD193 pKa = 4.66GSADD197 pKa = 3.83SLTDD201 pKa = 3.56QKK203 pKa = 11.07WVLNDD208 pKa = 3.06GDD210 pKa = 4.54YY211 pKa = 11.55VGGAANGNRR220 pKa = 11.84CYY222 pKa = 10.82GKK224 pKa = 10.43KK225 pKa = 10.33DD226 pKa = 3.32NLYY229 pKa = 9.68IDD231 pKa = 4.42GGNLVLKK238 pKa = 10.31VPKK241 pKa = 10.33DD242 pKa = 3.47QTASPNMEE250 pKa = 4.08CAEE253 pKa = 4.05IAFEE257 pKa = 4.22EE258 pKa = 4.59TNITGGIFQTTAMLSGVDD276 pKa = 3.98GTCQAFWLNHH286 pKa = 6.22SIATQYY292 pKa = 11.29ADD294 pKa = 3.45EE295 pKa = 4.37VDD297 pKa = 3.47IEE299 pKa = 4.8VISSTIDD306 pKa = 3.1TDD308 pKa = 4.93GIWYY312 pKa = 10.04SNWPPNGDD320 pKa = 3.89PNDD323 pKa = 4.12PDD325 pKa = 3.88NLVSAHH331 pKa = 5.96TNVAVPDD338 pKa = 3.99IDD340 pKa = 4.67SSDD343 pKa = 3.4PRR345 pKa = 11.84KK346 pKa = 8.58TYY348 pKa = 11.29NNYY351 pKa = 8.89TIAWLDD357 pKa = 3.54DD358 pKa = 3.44STNRR362 pKa = 11.84YY363 pKa = 9.31YY364 pKa = 11.21NGAKK368 pKa = 9.45QDD370 pKa = 3.97SPTEE374 pKa = 4.02NQPEE378 pKa = 4.09HH379 pKa = 8.82SMMFVINNWSNGGSGWTLGPPTGEE403 pKa = 4.4DD404 pKa = 2.87SLLKK408 pKa = 10.5VKK410 pKa = 10.62SVLLYY415 pKa = 10.9YY416 pKa = 9.43KK417 pKa = 9.41TANSTQMSDD426 pKa = 4.27LGGDD430 pKa = 3.74CQEE433 pKa = 4.88SDD435 pKa = 3.76VCTVV439 pKa = 2.84

Molecular weight: 45.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E3HCD1|A0A1E3HCD1_9TREE Uncharacterized protein OS=Cryptococcus amylolentus CBS 6039 OX=1295533 GN=L202_07487 PE=3 SV=1
MM1 pKa = 7.64SFLRR5 pKa = 11.84LNILRR10 pKa = 11.84PALSSSTPLPRR21 pKa = 11.84AQAFSSSSASSRR33 pKa = 11.84PSLLSLSPLSSASSSPLASLRR54 pKa = 11.84FRR56 pKa = 11.84SRR58 pKa = 11.84QQRR61 pKa = 11.84LPLGPGAVGGMGLGGSGRR79 pKa = 11.84RR80 pKa = 11.84EE81 pKa = 4.01FGWTRR86 pKa = 11.84VVEE89 pKa = 4.32ANPQKK94 pKa = 8.61QTKK97 pKa = 9.0LKK99 pKa = 10.09SHH101 pKa = 6.43SSSKK105 pKa = 10.62KK106 pKa = 9.97RR107 pKa = 11.84FFANAKK113 pKa = 10.08GMFKK117 pKa = 10.42RR118 pKa = 11.84AQAGKK123 pKa = 9.97AHH125 pKa = 7.18LNTPLSSSSINRR137 pKa = 11.84LAKK140 pKa = 9.93GVRR143 pKa = 11.84VTNTQARR150 pKa = 11.84KK151 pKa = 9.28LRR153 pKa = 11.84KK154 pKa = 9.17LLPFAA159 pKa = 5.4

Molecular weight: 17.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8246

1631

9877

4861164

41

4967

492.2

53.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.823 ± 0.022

1.008 ± 0.008

5.485 ± 0.019

6.508 ± 0.023

3.363 ± 0.016

7.406 ± 0.024

2.353 ± 0.011

4.444 ± 0.017

4.97 ± 0.022

8.872 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.09 ± 0.008

3.12 ± 0.012

6.925 ± 0.029

3.813 ± 0.018

5.95 ± 0.022

9.012 ± 0.033

5.764 ± 0.016

6.131 ± 0.017

1.413 ± 0.009

2.551 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski