Amino acid dipepetide frequency for Pseudomonas phage H72

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.373AlaAla: 18.373 ± 3.366
1.043AlaCys: 1.043 ± 0.348
6.739AlaAsp: 6.739 ± 0.587
8.745AlaGlu: 8.745 ± 0.774
3.129AlaPhe: 3.129 ± 0.494
10.27AlaGly: 10.27 ± 1.249
1.765AlaHis: 1.765 ± 0.488
5.616AlaIle: 5.616 ± 0.472
4.734AlaLys: 4.734 ± 0.823
12.677AlaLeu: 12.677 ± 1.06
3.37AlaMet: 3.37 ± 0.516
2.648AlaAsn: 2.648 ± 0.422
5.135AlaPro: 5.135 ± 0.674
6.418AlaGln: 6.418 ± 0.79
8.344AlaArg: 8.344 ± 0.967
7.301AlaSer: 7.301 ± 0.715
5.456AlaThr: 5.456 ± 0.905
6.418AlaVal: 6.418 ± 0.695
3.049AlaTrp: 3.049 ± 0.37
2.567AlaTyr: 2.567 ± 0.438
0.0AlaXaa: 0.0 ± 0.0
Cys
0.802CysAla: 0.802 ± 0.284
0.16CysCys: 0.16 ± 0.123
0.562CysAsp: 0.562 ± 0.203
0.481CysGlu: 0.481 ± 0.183
0.16CysPhe: 0.16 ± 0.112
0.802CysGly: 0.802 ± 0.269
0.241CysHis: 0.241 ± 0.136
0.241CysIle: 0.241 ± 0.145
0.321CysLys: 0.321 ± 0.17
0.481CysLeu: 0.481 ± 0.227
0.08CysMet: 0.08 ± 0.082
0.401CysAsn: 0.401 ± 0.158
0.481CysPro: 0.481 ± 0.177
0.401CysGln: 0.401 ± 0.164
0.963CysArg: 0.963 ± 0.351
0.722CysSer: 0.722 ± 0.22
0.321CysThr: 0.321 ± 0.185
0.722CysVal: 0.722 ± 0.271
0.0CysTrp: 0.0 ± 0.0
0.241CysTyr: 0.241 ± 0.136
0.0CysXaa: 0.0 ± 0.0
Asp
6.659AspAla: 6.659 ± 0.761
0.241AspCys: 0.241 ± 0.138
3.129AspAsp: 3.129 ± 0.455
4.252AspGlu: 4.252 ± 0.504
2.407AspPhe: 2.407 ± 0.487
5.777AspGly: 5.777 ± 0.618
1.203AspHis: 1.203 ± 0.379
2.888AspIle: 2.888 ± 0.468
1.524AspLys: 1.524 ± 0.29
5.375AspLeu: 5.375 ± 0.512
0.802AspMet: 0.802 ± 0.229
1.444AspAsn: 1.444 ± 0.299
2.808AspPro: 2.808 ± 0.431
3.61AspGln: 3.61 ± 0.644
3.45AspArg: 3.45 ± 0.591
2.808AspSer: 2.808 ± 0.415
2.567AspThr: 2.567 ± 0.444
4.734AspVal: 4.734 ± 0.607
0.802AspTrp: 0.802 ± 0.252
1.605AspTyr: 1.605 ± 0.347
0.0AspXaa: 0.0 ± 0.0
Glu
8.023GluAla: 8.023 ± 1.058
0.802GluCys: 0.802 ± 0.257
3.771GluAsp: 3.771 ± 0.602
4.012GluGlu: 4.012 ± 0.603
1.685GluPhe: 1.685 ± 0.402
2.327GluGly: 2.327 ± 0.457
1.605GluHis: 1.605 ± 0.444
2.648GluIle: 2.648 ± 0.429
3.45GluLys: 3.45 ± 0.697
7.622GluLeu: 7.622 ± 0.744
1.203GluMet: 1.203 ± 0.374
2.166GluAsn: 2.166 ± 0.38
1.685GluPro: 1.685 ± 0.447
4.894GluGln: 4.894 ± 0.696
5.777GluArg: 5.777 ± 0.777
3.53GluSer: 3.53 ± 0.685
2.166GluThr: 2.166 ± 0.283
3.289GluVal: 3.289 ± 0.596
1.203GluTrp: 1.203 ± 0.297
1.926GluTyr: 1.926 ± 0.3
0.0GluXaa: 0.0 ± 0.0
Phe
2.969PheAla: 2.969 ± 0.531
0.321PheCys: 0.321 ± 0.181
2.648PheAsp: 2.648 ± 0.607
1.926PheGlu: 1.926 ± 0.343
0.642PhePhe: 0.642 ± 0.199
2.969PheGly: 2.969 ± 0.441
0.401PheHis: 0.401 ± 0.15
1.123PheIle: 1.123 ± 0.26
0.562PheLys: 0.562 ± 0.21
2.487PheLeu: 2.487 ± 0.398
0.642PheMet: 0.642 ± 0.178
1.444PheAsn: 1.444 ± 0.409
1.123PhePro: 1.123 ± 0.299
1.284PheGln: 1.284 ± 0.332
2.728PheArg: 2.728 ± 0.526
1.284PheSer: 1.284 ± 0.278
1.605PheThr: 1.605 ± 0.395
1.364PheVal: 1.364 ± 0.27
0.481PheTrp: 0.481 ± 0.199
0.562PheTyr: 0.562 ± 0.214
0.0PheXaa: 0.0 ± 0.0
Gly
8.585GlyAla: 8.585 ± 1.486
0.883GlyCys: 0.883 ± 0.3
4.172GlyAsp: 4.172 ± 0.58
3.931GlyGlu: 3.931 ± 0.393
2.407GlyPhe: 2.407 ± 0.389
5.135GlyGly: 5.135 ± 0.626
1.203GlyHis: 1.203 ± 0.337
3.129GlyIle: 3.129 ± 0.492
4.252GlyLys: 4.252 ± 0.633
7.943GlyLeu: 7.943 ± 0.89
2.086GlyMet: 2.086 ± 0.362
2.728GlyAsn: 2.728 ± 0.564
2.327GlyPro: 2.327 ± 0.47
4.573GlyGln: 4.573 ± 0.568
4.653GlyArg: 4.653 ± 0.647
3.53GlySer: 3.53 ± 0.533
4.573GlyThr: 4.573 ± 0.699
5.616GlyVal: 5.616 ± 0.695
2.006GlyTrp: 2.006 ± 0.321
2.246GlyTyr: 2.246 ± 0.447
0.0GlyXaa: 0.0 ± 0.0
His
1.926HisAla: 1.926 ± 0.466
0.08HisCys: 0.08 ± 0.065
0.883HisAsp: 0.883 ± 0.232
1.123HisGlu: 1.123 ± 0.246
0.321HisPhe: 0.321 ± 0.133
1.685HisGly: 1.685 ± 0.448
0.321HisHis: 0.321 ± 0.155
0.722HisIle: 0.722 ± 0.242
0.802HisLys: 0.802 ± 0.243
2.086HisLeu: 2.086 ± 0.483
0.16HisMet: 0.16 ± 0.102
0.802HisAsn: 0.802 ± 0.233
0.963HisPro: 0.963 ± 0.38
1.364HisGln: 1.364 ± 0.402
1.284HisArg: 1.284 ± 0.268
1.123HisSer: 1.123 ± 0.265
0.722HisThr: 0.722 ± 0.216
1.043HisVal: 1.043 ± 0.296
0.241HisTrp: 0.241 ± 0.138
0.16HisTyr: 0.16 ± 0.114
0.0HisXaa: 0.0 ± 0.0
Ile
5.456IleAla: 5.456 ± 0.661
0.321IleCys: 0.321 ± 0.192
2.648IleAsp: 2.648 ± 0.357
4.332IleGlu: 4.332 ± 0.592
0.722IlePhe: 0.722 ± 0.194
2.407IleGly: 2.407 ± 0.468
0.802IleHis: 0.802 ± 0.217
1.203IleIle: 1.203 ± 0.425
1.284IleLys: 1.284 ± 0.281
3.129IleLeu: 3.129 ± 0.456
0.401IleMet: 0.401 ± 0.197
1.444IleAsn: 1.444 ± 0.313
1.926IlePro: 1.926 ± 0.36
1.845IleGln: 1.845 ± 0.395
3.37IleArg: 3.37 ± 0.538
2.728IleSer: 2.728 ± 0.546
3.37IleThr: 3.37 ± 0.563
1.685IleVal: 1.685 ± 0.382
0.722IleTrp: 0.722 ± 0.316
1.043IleTyr: 1.043 ± 0.287
0.0IleXaa: 0.0 ± 0.0
Lys
5.857LysAla: 5.857 ± 0.866
0.241LysCys: 0.241 ± 0.155
2.246LysAsp: 2.246 ± 0.437
2.567LysGlu: 2.567 ± 0.518
0.963LysPhe: 0.963 ± 0.236
2.487LysGly: 2.487 ± 0.526
0.562LysHis: 0.562 ± 0.171
1.284LysIle: 1.284 ± 0.321
1.765LysLys: 1.765 ± 0.319
3.931LysLeu: 3.931 ± 0.812
0.401LysMet: 0.401 ± 0.159
1.203LysAsn: 1.203 ± 0.264
2.086LysPro: 2.086 ± 0.619
1.524LysGln: 1.524 ± 0.373
4.493LysArg: 4.493 ± 0.778
2.327LysSer: 2.327 ± 0.462
1.845LysThr: 1.845 ± 0.407
2.086LysVal: 2.086 ± 0.416
0.321LysTrp: 0.321 ± 0.149
0.722LysTyr: 0.722 ± 0.175
0.0LysXaa: 0.0 ± 0.0
Leu
12.115LeuAla: 12.115 ± 0.938
1.123LeuCys: 1.123 ± 0.337
7.461LeuAsp: 7.461 ± 0.67
5.295LeuGlu: 5.295 ± 0.605
2.246LeuPhe: 2.246 ± 0.487
7.381LeuGly: 7.381 ± 0.765
1.765LeuHis: 1.765 ± 0.342
3.691LeuIle: 3.691 ± 0.548
3.931LeuLys: 3.931 ± 0.624
9.146LeuLeu: 9.146 ± 0.893
2.246LeuMet: 2.246 ± 0.358
3.209LeuAsn: 3.209 ± 0.446
5.295LeuPro: 5.295 ± 0.706
5.777LeuGln: 5.777 ± 0.84
8.023LeuArg: 8.023 ± 0.859
6.178LeuSer: 6.178 ± 0.645
6.82LeuThr: 6.82 ± 0.735
7.622LeuVal: 7.622 ± 0.751
0.481LeuTrp: 0.481 ± 0.17
2.086LeuTyr: 2.086 ± 0.453
0.0LeuXaa: 0.0 ± 0.0
Met
2.728MetAla: 2.728 ± 0.456
0.0MetCys: 0.0 ± 0.0
1.203MetAsp: 1.203 ± 0.334
1.685MetGlu: 1.685 ± 0.374
0.642MetPhe: 0.642 ± 0.163
2.086MetGly: 2.086 ± 0.365
0.401MetHis: 0.401 ± 0.196
1.203MetIle: 1.203 ± 0.343
0.963MetLys: 0.963 ± 0.249
1.845MetLeu: 1.845 ± 0.442
0.08MetMet: 0.08 ± 0.076
0.802MetAsn: 0.802 ± 0.358
1.043MetPro: 1.043 ± 0.218
0.963MetGln: 0.963 ± 0.27
1.203MetArg: 1.203 ± 0.289
1.765MetSer: 1.765 ± 0.303
1.524MetThr: 1.524 ± 0.43
0.963MetVal: 0.963 ± 0.266
0.16MetTrp: 0.16 ± 0.119
0.0MetTyr: 0.0 ± 0.0
0.0MetXaa: 0.0 ± 0.0
Asn
3.61AsnAla: 3.61 ± 0.617
0.16AsnCys: 0.16 ± 0.175
1.203AsnAsp: 1.203 ± 0.259
1.926AsnGlu: 1.926 ± 0.331
0.642AsnPhe: 0.642 ± 0.183
2.648AsnGly: 2.648 ± 0.395
0.802AsnHis: 0.802 ± 0.267
0.722AsnIle: 0.722 ± 0.246
0.963AsnLys: 0.963 ± 0.255
3.37AsnLeu: 3.37 ± 0.572
0.481AsnMet: 0.481 ± 0.192
1.043AsnAsn: 1.043 ± 0.409
1.765AsnPro: 1.765 ± 0.429
1.926AsnGln: 1.926 ± 0.415
2.487AsnArg: 2.487 ± 0.399
1.284AsnSer: 1.284 ± 0.292
1.926AsnThr: 1.926 ± 0.485
1.444AsnVal: 1.444 ± 0.261
0.722AsnTrp: 0.722 ± 0.263
1.043AsnTyr: 1.043 ± 0.251
0.0AsnXaa: 0.0 ± 0.0
Pro
6.659ProAla: 6.659 ± 0.72
0.16ProCys: 0.16 ± 0.109
2.888ProAsp: 2.888 ± 0.563
2.888ProGlu: 2.888 ± 0.433
1.765ProPhe: 1.765 ± 0.479
4.573ProGly: 4.573 ± 0.737
0.481ProHis: 0.481 ± 0.188
1.524ProIle: 1.524 ± 0.336
1.444ProLys: 1.444 ± 0.364
3.691ProLeu: 3.691 ± 0.722
1.364ProMet: 1.364 ± 0.342
1.123ProAsn: 1.123 ± 0.236
2.246ProPro: 2.246 ± 0.532
1.765ProGln: 1.765 ± 0.316
4.012ProArg: 4.012 ± 0.465
3.209ProSer: 3.209 ± 0.5
2.728ProThr: 2.728 ± 0.331
3.53ProVal: 3.53 ± 0.653
0.883ProTrp: 0.883 ± 0.299
1.685ProTyr: 1.685 ± 0.401
0.0ProXaa: 0.0 ± 0.0
Gln
6.98GlnAla: 6.98 ± 0.981
0.401GlnCys: 0.401 ± 0.203
2.407GlnAsp: 2.407 ± 0.45
3.129GlnGlu: 3.129 ± 0.774
1.845GlnPhe: 1.845 ± 0.343
2.487GlnGly: 2.487 ± 0.446
0.963GlnHis: 0.963 ± 0.288
1.845GlnIle: 1.845 ± 0.289
1.765GlnLys: 1.765 ± 0.65
7.301GlnLeu: 7.301 ± 0.753
0.963GlnMet: 0.963 ± 0.271
0.802GlnAsn: 0.802 ± 0.31
3.37GlnPro: 3.37 ± 0.526
3.53GlnGln: 3.53 ± 0.593
3.61GlnArg: 3.61 ± 0.539
2.327GlnSer: 2.327 ± 0.403
2.567GlnThr: 2.567 ± 0.438
3.61GlnVal: 3.61 ± 0.421
0.802GlnTrp: 0.802 ± 0.216
1.123GlnTyr: 1.123 ± 0.452
0.0GlnXaa: 0.0 ± 0.0
Arg
7.863ArgAla: 7.863 ± 0.659
0.642ArgCys: 0.642 ± 0.237
4.734ArgAsp: 4.734 ± 0.474
4.734ArgGlu: 4.734 ± 0.63
2.006ArgPhe: 2.006 ± 0.378
5.616ArgGly: 5.616 ± 0.54
1.845ArgHis: 1.845 ± 0.434
4.092ArgIle: 4.092 ± 0.608
3.289ArgLys: 3.289 ± 0.782
8.424ArgLeu: 8.424 ± 0.59
1.765ArgMet: 1.765 ± 0.343
1.926ArgAsn: 1.926 ± 0.411
3.129ArgPro: 3.129 ± 0.671
3.61ArgGln: 3.61 ± 0.656
6.178ArgArg: 6.178 ± 0.858
4.252ArgSer: 4.252 ± 0.56
2.407ArgThr: 2.407 ± 0.512
4.172ArgVal: 4.172 ± 0.664
1.685ArgTrp: 1.685 ± 0.468
2.166ArgTyr: 2.166 ± 0.414
0.0ArgXaa: 0.0 ± 0.0
Ser
8.103SerAla: 8.103 ± 0.874
0.481SerCys: 0.481 ± 0.193
3.61SerAsp: 3.61 ± 0.459
3.37SerGlu: 3.37 ± 0.464
2.246SerPhe: 2.246 ± 0.368
4.493SerGly: 4.493 ± 0.482
0.562SerHis: 0.562 ± 0.179
2.327SerIle: 2.327 ± 0.373
2.327SerLys: 2.327 ± 0.488
6.178SerLeu: 6.178 ± 0.625
1.364SerMet: 1.364 ± 0.347
1.685SerAsn: 1.685 ± 0.309
3.129SerPro: 3.129 ± 0.632
1.685SerGln: 1.685 ± 0.305
3.771SerArg: 3.771 ± 0.591
3.209SerSer: 3.209 ± 0.572
3.53SerThr: 3.53 ± 0.511
3.53SerVal: 3.53 ± 0.609
0.802SerTrp: 0.802 ± 0.294
1.524SerTyr: 1.524 ± 0.315
0.0SerXaa: 0.0 ± 0.0
Thr
6.659ThrAla: 6.659 ± 0.873
0.401ThrCys: 0.401 ± 0.185
2.006ThrAsp: 2.006 ± 0.387
2.648ThrGlu: 2.648 ± 0.452
1.444ThrPhe: 1.444 ± 0.31
6.017ThrGly: 6.017 ± 1.005
1.043ThrHis: 1.043 ± 0.245
1.765ThrIle: 1.765 ± 0.361
1.685ThrLys: 1.685 ± 0.368
5.135ThrLeu: 5.135 ± 0.702
1.043ThrMet: 1.043 ± 0.295
2.006ThrAsn: 2.006 ± 0.46
4.012ThrPro: 4.012 ± 0.53
1.524ThrGln: 1.524 ± 0.374
3.049ThrArg: 3.049 ± 0.45
4.092ThrSer: 4.092 ± 0.567
2.808ThrThr: 2.808 ± 0.495
3.771ThrVal: 3.771 ± 0.601
0.802ThrTrp: 0.802 ± 0.303
1.364ThrTyr: 1.364 ± 0.379
0.0ThrXaa: 0.0 ± 0.0
Val
6.659ValAla: 6.659 ± 0.763
0.642ValCys: 0.642 ± 0.207
4.092ValAsp: 4.092 ± 0.525
4.653ValGlu: 4.653 ± 0.656
1.845ValPhe: 1.845 ± 0.342
3.851ValGly: 3.851 ± 0.5
1.043ValHis: 1.043 ± 0.316
2.969ValIle: 2.969 ± 0.478
2.487ValLys: 2.487 ± 0.454
6.579ValLeu: 6.579 ± 0.702
1.203ValMet: 1.203 ± 0.335
2.006ValAsn: 2.006 ± 0.398
3.771ValPro: 3.771 ± 0.547
3.209ValGln: 3.209 ± 0.576
3.53ValArg: 3.53 ± 0.538
3.45ValSer: 3.45 ± 0.622
3.931ValThr: 3.931 ± 0.631
4.734ValVal: 4.734 ± 0.847
1.364ValTrp: 1.364 ± 0.383
1.043ValTyr: 1.043 ± 0.291
0.0ValXaa: 0.0 ± 0.0
Trp
1.444TrpAla: 1.444 ± 0.36
0.321TrpCys: 0.321 ± 0.149
0.642TrpAsp: 0.642 ± 0.218
0.241TrpGlu: 0.241 ± 0.128
0.883TrpPhe: 0.883 ± 0.222
0.963TrpGly: 0.963 ± 0.299
0.321TrpHis: 0.321 ± 0.161
1.123TrpIle: 1.123 ± 0.261
0.401TrpLys: 0.401 ± 0.175
2.246TrpLeu: 2.246 ± 0.445
0.722TrpMet: 0.722 ± 0.268
0.481TrpAsn: 0.481 ± 0.195
0.802TrpPro: 0.802 ± 0.407
1.203TrpGln: 1.203 ± 0.298
1.524TrpArg: 1.524 ± 0.268
1.123TrpSer: 1.123 ± 0.292
1.043TrpThr: 1.043 ± 0.303
1.123TrpVal: 1.123 ± 0.336
0.642TrpTrp: 0.642 ± 0.293
0.241TrpTyr: 0.241 ± 0.129
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.327TyrAla: 2.327 ± 0.371
0.16TyrCys: 0.16 ± 0.131
1.043TyrAsp: 1.043 ± 0.27
1.845TyrGlu: 1.845 ± 0.374
0.562TyrPhe: 0.562 ± 0.18
2.006TyrGly: 2.006 ± 0.423
0.481TyrHis: 0.481 ± 0.286
0.722TyrIle: 0.722 ± 0.271
0.963TyrLys: 0.963 ± 0.282
2.246TyrLeu: 2.246 ± 0.407
0.802TyrMet: 0.802 ± 0.282
0.802TyrAsn: 0.802 ± 0.276
1.524TyrPro: 1.524 ± 0.474
0.802TyrGln: 0.802 ± 0.216
2.086TyrArg: 2.086 ± 0.305
1.685TyrSer: 1.685 ± 0.331
1.364TyrThr: 1.364 ± 0.379
1.605TyrVal: 1.605 ± 0.319
0.321TyrTrp: 0.321 ± 0.162
0.241TyrTyr: 0.241 ± 0.14
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (12465 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski