Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae; Pyrenophora; Pyrenophora teres; Pyrenophora teres f. teres

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11705 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3RET1|E3RET1_PYRTT Uncharacterized protein OS=Pyrenophora teres f. teres (strain 0-1) OX=861557 GN=PTT_04958 PE=4 SV=1
MM1 pKa = 7.75HH2 pKa = 7.81DD3 pKa = 3.91AGLVSHH9 pKa = 7.47DD10 pKa = 4.73PSPGPPVRR18 pKa = 11.84TFVHH22 pKa = 6.11NNDD25 pKa = 3.2VPFYY29 pKa = 8.98PAPDD33 pKa = 4.37DD34 pKa = 4.81SLLDD38 pKa = 4.16DD39 pKa = 5.42DD40 pKa = 5.82APPSYY45 pKa = 10.3EE46 pKa = 3.9DD47 pKa = 3.24TVGIVGAPPGYY58 pKa = 9.13GTFRR62 pKa = 11.84PYY64 pKa = 10.43TEE66 pKa = 4.3EE67 pKa = 3.95SSIASSEE74 pKa = 4.28VEE76 pKa = 4.05SSDD79 pKa = 3.16RR80 pKa = 11.84ALPEE84 pKa = 3.78WVGQGLVVFIFISIIYY100 pKa = 8.13GFWRR104 pKa = 11.84FINAPDD110 pKa = 4.17DD111 pKa = 3.97LSPIGVWPGPPPPPP125 pKa = 4.36

Molecular weight: 13.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3S5A5|E3S5A5_PYRTT Uncharacterized protein OS=Pyrenophora teres f. teres (strain 0-1) OX=861557 GN=PTT_17803 PE=4 SV=1
MM1 pKa = 7.94PSQKK5 pKa = 10.41SFRR8 pKa = 11.84TKK10 pKa = 10.33QKK12 pKa = 10.05LARR15 pKa = 11.84AQKK18 pKa = 8.97QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.14WRR45 pKa = 11.84KK46 pKa = 7.41TRR48 pKa = 11.84LGII51 pKa = 4.46

Molecular weight: 6.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11705

0

11705

5424802

50

9819

463.5

51.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.755 ± 0.021

1.163 ± 0.009

5.737 ± 0.016

6.283 ± 0.027

3.6 ± 0.015

6.685 ± 0.025

2.439 ± 0.011

4.837 ± 0.019

5.217 ± 0.023

8.518 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.285 ± 0.009

3.785 ± 0.012

6.147 ± 0.027

4.171 ± 0.019

5.962 ± 0.021

7.968 ± 0.027

6.18 ± 0.016

6.031 ± 0.019

1.417 ± 0.009

2.821 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski