Discula destructiva virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; Gammapartitivirus

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q91HI8|Q91HI8_9VIRU RNA-dependent RNA polymerase OS=Discula destructiva virus 2 OX=160484 PE=4 SV=1
MM1 pKa = 7.69EE2 pKa = 6.06GFTQEE7 pKa = 4.09PTNTTVLAEE16 pKa = 4.2EE17 pKa = 4.25LHH19 pKa = 7.05SVDD22 pKa = 4.03TLHH25 pKa = 7.0LRR27 pKa = 11.84PGKK30 pKa = 8.56TRR32 pKa = 11.84STTSEE37 pKa = 3.76DD38 pKa = 3.45VLPNNYY44 pKa = 9.04EE45 pKa = 4.07DD46 pKa = 3.66PCLRR50 pKa = 11.84EE51 pKa = 3.35IAKK54 pKa = 10.49YY55 pKa = 10.25GGYY58 pKa = 7.94STYY61 pKa = 11.11SSNSNTDD68 pKa = 2.65PWIRR72 pKa = 11.84EE73 pKa = 3.95TLKK76 pKa = 10.91LHH78 pKa = 7.03DD79 pKa = 3.79RR80 pKa = 11.84QIYY83 pKa = 8.79EE84 pKa = 4.81DD85 pKa = 2.92IWGKK89 pKa = 6.99TRR91 pKa = 11.84RR92 pKa = 11.84PEE94 pKa = 4.14GTPGMYY100 pKa = 9.9KK101 pKa = 10.46ALGRR105 pKa = 11.84FGGEE109 pKa = 3.08RR110 pKa = 11.84CGFDD114 pKa = 5.05DD115 pKa = 6.4LSSQQKK121 pKa = 9.74SSMRR125 pKa = 11.84RR126 pKa = 11.84AIAKK130 pKa = 9.38AKK132 pKa = 10.28KK133 pKa = 9.98AFKK136 pKa = 10.34LPYY139 pKa = 9.55KK140 pKa = 10.34RR141 pKa = 11.84EE142 pKa = 3.91PLDD145 pKa = 3.15WHH147 pKa = 6.48EE148 pKa = 4.21VGQFLRR154 pKa = 11.84RR155 pKa = 11.84DD156 pKa = 3.54TSAGSTFMGSKK167 pKa = 10.02KK168 pKa = 10.11GDD170 pKa = 3.51VMEE173 pKa = 5.35EE174 pKa = 4.02IYY176 pKa = 10.94SEE178 pKa = 4.21ARR180 pKa = 11.84WLGHH184 pKa = 6.32RR185 pKa = 11.84MKK187 pKa = 10.64QDD189 pKa = 2.55GRR191 pKa = 11.84SRR193 pKa = 11.84FDD195 pKa = 3.2PTKK198 pKa = 10.31MRR200 pKa = 11.84FPPCLAGQRR209 pKa = 11.84GGMSDD214 pKa = 3.86RR215 pKa = 11.84DD216 pKa = 3.83DD217 pKa = 3.97PKK219 pKa = 11.11TRR221 pKa = 11.84LIWIYY226 pKa = 9.13PAEE229 pKa = 4.11MLCVEE234 pKa = 5.06GFYY237 pKa = 11.1APLMYY242 pKa = 9.21RR243 pKa = 11.84DD244 pKa = 4.18YY245 pKa = 10.85MSDD248 pKa = 3.43PNSPMLNGKK257 pKa = 9.37SSQRR261 pKa = 11.84LYY263 pKa = 10.77TEE265 pKa = 4.24WCCNLRR271 pKa = 11.84EE272 pKa = 4.15GEE274 pKa = 4.27TLYY277 pKa = 11.45GIDD280 pKa = 4.95FSAFDD285 pKa = 3.86SKK287 pKa = 11.33VPAWLIRR294 pKa = 11.84TAFDD298 pKa = 3.79IVKK301 pKa = 10.36QNINFEE307 pKa = 4.33TFEE310 pKa = 4.31GKK312 pKa = 9.17PVNKK316 pKa = 9.76VDD318 pKa = 3.27AQKK321 pKa = 10.5WKK323 pKa = 10.38NVWDD327 pKa = 3.61AMVWYY332 pKa = 8.66FINTPILMPDD342 pKa = 2.41GRR344 pKa = 11.84MFRR347 pKa = 11.84KK348 pKa = 9.98YY349 pKa = 10.14RR350 pKa = 11.84GVPSGSWWTQIIDD363 pKa = 3.75SVVNNILIDD372 pKa = 3.85YY373 pKa = 9.9LADD376 pKa = 3.91CQSVKK381 pKa = 10.15IPKK384 pKa = 9.84PEE386 pKa = 3.83VLGDD390 pKa = 3.64DD391 pKa = 3.6SAFRR395 pKa = 11.84SNDD398 pKa = 3.32QFDD401 pKa = 4.65LEE403 pKa = 4.49VAKK406 pKa = 10.44DD407 pKa = 3.64DD408 pKa = 4.05CVPTGMVIRR417 pKa = 11.84PEE419 pKa = 4.03KK420 pKa = 10.4CEE422 pKa = 3.91KK423 pKa = 10.45TEE425 pKa = 4.52DD426 pKa = 3.63PAEE429 pKa = 4.22FKK431 pKa = 11.21LLGTKK436 pKa = 9.45YY437 pKa = 10.21RR438 pKa = 11.84SGRR441 pKa = 11.84VHH443 pKa = 7.52RR444 pKa = 11.84STDD447 pKa = 2.79EE448 pKa = 3.96WFSLALYY455 pKa = 9.44PEE457 pKa = 4.56SSVLSLDD464 pKa = 3.43VSFTRR469 pKa = 11.84LVGLWLGGAMWDD481 pKa = 3.56KK482 pKa = 10.66QFCEE486 pKa = 4.26FMDD489 pKa = 4.49YY490 pKa = 10.98YY491 pKa = 9.16QTSYY495 pKa = 10.78PVPEE499 pKa = 4.77EE500 pKa = 3.98GWFSKK505 pKa = 10.13DD506 pKa = 3.02QKK508 pKa = 10.51RR509 pKa = 11.84WLEE512 pKa = 3.89IIYY515 pKa = 10.23SGKK518 pKa = 10.28APRR521 pKa = 11.84GWTTKK526 pKa = 10.52KK527 pKa = 10.35SLFWRR532 pKa = 11.84SIFYY536 pKa = 10.51AYY538 pKa = 10.44GG539 pKa = 3.3

Molecular weight: 62.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q91HI8|Q91HI8_9VIRU RNA-dependent RNA polymerase OS=Discula destructiva virus 2 OX=160484 PE=4 SV=1
MM1 pKa = 8.05ADD3 pKa = 3.19TSSNIPSSVTPNDD16 pKa = 3.55SVSNSGKK23 pKa = 10.13RR24 pKa = 11.84KK25 pKa = 9.1SKK27 pKa = 9.65PGKK30 pKa = 9.85AEE32 pKa = 3.59RR33 pKa = 11.84LARR36 pKa = 11.84RR37 pKa = 11.84SAVGSQPGQAASASKK52 pKa = 10.74AAMFSSGSMAPKK64 pKa = 9.01PQPGKK69 pKa = 10.6YY70 pKa = 9.01PVVFQTGAGEE80 pKa = 4.06PSRR83 pKa = 11.84DD84 pKa = 3.42QQFAISGPTISKK96 pKa = 8.61TLEE99 pKa = 4.01GFPEE103 pKa = 4.03RR104 pKa = 11.84FSFSEE109 pKa = 3.99KK110 pKa = 9.22FTEE113 pKa = 5.35FKK115 pKa = 11.03ANSGFDD121 pKa = 4.04DD122 pKa = 4.26EE123 pKa = 6.08DD124 pKa = 4.43FEE126 pKa = 6.18LDD128 pKa = 3.38IVVSTLLRR136 pKa = 11.84LSQQVVHH143 pKa = 5.79SHH145 pKa = 5.44VNMGLPQGDD154 pKa = 4.3FAPVASTEE162 pKa = 3.82VRR164 pKa = 11.84VPGSVAAFVEE174 pKa = 4.44QFGEE178 pKa = 4.17HH179 pKa = 5.91SVPAIGTRR187 pKa = 11.84FLFKK191 pKa = 10.39DD192 pKa = 3.28YY193 pKa = 10.4RR194 pKa = 11.84QTVSRR199 pKa = 11.84LVWAAEE205 pKa = 3.69QAAVGKK211 pKa = 9.22WKK213 pKa = 10.65EE214 pKa = 3.7PLEE217 pKa = 4.16RR218 pKa = 11.84LWLPMSSSDD227 pKa = 3.05GHH229 pKa = 5.91TKK231 pKa = 10.86LEE233 pKa = 3.9IAHH236 pKa = 6.97RR237 pKa = 11.84LNAFLEE243 pKa = 4.29NAEE246 pKa = 4.02VQIPYY251 pKa = 10.34SILEE255 pKa = 4.54DD256 pKa = 3.78GVLSGTVPDD265 pKa = 3.59AWNGIKK271 pKa = 10.55GFLGDD276 pKa = 4.14PPTPGGVDD284 pKa = 2.75RR285 pKa = 11.84RR286 pKa = 11.84DD287 pKa = 3.29RR288 pKa = 11.84FDD290 pKa = 5.28FLFKK294 pKa = 10.57SYY296 pKa = 11.41NDD298 pKa = 3.7APQLAVAFTTAAATAVLGEE317 pKa = 4.61LRR319 pKa = 11.84LPWSAPSAGHH329 pKa = 6.73LNWSFNARR337 pKa = 11.84EE338 pKa = 4.01AFTRR342 pKa = 11.84LSDD345 pKa = 2.8NWARR349 pKa = 11.84KK350 pKa = 6.88STTYY354 pKa = 11.35AKK356 pKa = 10.34FFEE359 pKa = 4.91LSSSLSNRR367 pKa = 11.84TAATGSQSQMALVSEE382 pKa = 4.46SDD384 pKa = 3.77GITVVKK390 pKa = 9.07TALALSPPEE399 pKa = 4.09FSLVACFPASCLYY412 pKa = 10.66SGEE415 pKa = 3.96LVRR418 pKa = 11.84RR419 pKa = 11.84VVVTTPLSVRR429 pKa = 11.84QRR431 pKa = 11.84ATEE434 pKa = 4.17FVQMDD439 pKa = 3.24WRR441 pKa = 11.84AA442 pKa = 2.9

Molecular weight: 47.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

981

442

539

490.5

55.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.441 ± 2.045

1.223 ± 0.461

6.014 ± 0.891

6.116 ± 0.275

5.505 ± 0.497

7.034 ± 0.147

1.223 ± 0.08

3.364 ± 0.659

5.708 ± 0.843

7.441 ± 0.15

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.548 ± 0.577

2.956 ± 0.009

6.116 ± 0.266

3.466 ± 0.363

6.524 ± 0.384

9.48 ± 1.367

5.81 ± 0.043

6.116 ± 1.078

2.752 ± 0.564

3.16 ± 1.078
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski