Helicobacter cholecystus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Helicobacteraceae; Helicobacter

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1316 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3D8IWC4|A0A3D8IWC4_9HELI Flagellar basal-body rod protein FlgC OS=Helicobacter cholecystus OX=45498 GN=flgC PE=3 SV=1
MM1 pKa = 7.28SVKK4 pKa = 9.26ITDD7 pKa = 3.01ICIACGSCIDD17 pKa = 4.12EE18 pKa = 4.92CPVSAIVDD26 pKa = 3.67DD27 pKa = 4.88DD28 pKa = 5.03DD29 pKa = 4.99NPEE32 pKa = 4.1GTGTYY37 pKa = 9.84YY38 pKa = 10.96VYY40 pKa = 11.13ADD42 pKa = 3.69KK43 pKa = 11.13CVEE46 pKa = 4.3CVGHH50 pKa = 6.61NDD52 pKa = 3.28EE53 pKa = 4.91PACASACPTDD63 pKa = 4.15GCIVWSAVGSVSRR76 pKa = 11.84DD77 pKa = 3.3SIGADD82 pKa = 3.28LRR84 pKa = 11.84DD85 pKa = 3.87GTHH88 pKa = 6.57PVCC91 pKa = 5.74

Molecular weight: 9.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3D8IU47|A0A3D8IU47_9HELI MFS transporter OS=Helicobacter cholecystus OX=45498 GN=yajR PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.04QPHH8 pKa = 4.86NTPRR12 pKa = 11.84KK13 pKa = 7.31RR14 pKa = 11.84THH16 pKa = 5.88GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.07TKK25 pKa = 10.39NGRR28 pKa = 11.84KK29 pKa = 9.3VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.41GRR39 pKa = 11.84KK40 pKa = 8.68RR41 pKa = 11.84LSVV44 pKa = 3.2

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1316

0

1316

422834

37

2881

321.3

36.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.412 ± 0.059

1.22 ± 0.034

4.561 ± 0.044

6.859 ± 0.082

5.276 ± 0.059

6.668 ± 0.078

1.846 ± 0.034

8.72 ± 0.058

7.872 ± 0.063

10.568 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.258 ± 0.027

5.133 ± 0.093

3.237 ± 0.047

4.028 ± 0.043

3.552 ± 0.042

7.32 ± 0.072

4.565 ± 0.086

5.372 ± 0.054

0.742 ± 0.021

3.793 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski