Bacteroides sp. CAG:714

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; environmental samples

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2561 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6YIY8|R6YIY8_9BACE tRNA(Ile)-lysidine synthase OS=Bacteroides sp. CAG:714 OX=1262749 GN=tilS PE=3 SV=1
MM1 pKa = 7.4KK2 pKa = 10.2KK3 pKa = 9.79IGIIYY8 pKa = 10.07GSTTGTCEE16 pKa = 3.9SLANQLATCLGVSDD30 pKa = 5.01NDD32 pKa = 3.27VHH34 pKa = 7.89SADD37 pKa = 4.29KK38 pKa = 10.24LTDD41 pKa = 3.88ALVSAYY47 pKa = 10.25DD48 pKa = 3.65VLILGTSTWGDD59 pKa = 3.63GEE61 pKa = 5.21LQDD64 pKa = 5.3DD65 pKa = 4.5WYY67 pKa = 10.86DD68 pKa = 3.84GIKK71 pKa = 10.23VLKK74 pKa = 10.34QADD77 pKa = 4.16LNGKK81 pKa = 6.73TVALFGCGDD90 pKa = 3.62SEE92 pKa = 4.97AYY94 pKa = 10.47CDD96 pKa = 3.95TFCDD100 pKa = 3.78GMGILYY106 pKa = 10.09EE107 pKa = 4.23DD108 pKa = 4.85LKK110 pKa = 11.54DD111 pKa = 3.79SGCTFLGNRR120 pKa = 11.84VSTDD124 pKa = 3.19DD125 pKa = 3.6YY126 pKa = 10.73TFSSSIAVVDD136 pKa = 3.94GTFVGLALDD145 pKa = 4.84DD146 pKa = 4.21VNEE149 pKa = 4.25SAKK152 pKa = 9.75TAGRR156 pKa = 11.84IQAWADD162 pKa = 3.65EE163 pKa = 4.84LKK165 pKa = 10.92HH166 pKa = 6.48SLL168 pKa = 3.97

Molecular weight: 17.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6YYC1|R6YYC1_9BACE Uncharacterized protein OS=Bacteroides sp. CAG:714 OX=1262749 GN=BN762_01225 PE=4 SV=1
MM1 pKa = 7.67PMATQRR7 pKa = 11.84ILADD11 pKa = 3.54EE12 pKa = 4.49DD13 pKa = 3.36FGRR16 pKa = 11.84IVIRR20 pKa = 11.84TRR22 pKa = 11.84RR23 pKa = 11.84TARR26 pKa = 11.84NISMRR31 pKa = 11.84VRR33 pKa = 11.84PEE35 pKa = 3.36GLYY38 pKa = 10.48VVVPPFSRR46 pKa = 11.84ADD48 pKa = 3.58KK49 pKa = 10.08ILEE52 pKa = 4.55AIAPFRR58 pKa = 11.84PRR60 pKa = 11.84LLEE63 pKa = 3.82SFRR66 pKa = 11.84KK67 pKa = 9.28VAVRR71 pKa = 11.84LIDD74 pKa = 3.84FNFSIQADD82 pKa = 4.22CFRR85 pKa = 11.84LSLVPSRR92 pKa = 11.84LRR94 pKa = 11.84CFTVKK99 pKa = 10.71EE100 pKa = 4.16EE101 pKa = 4.33GEE103 pKa = 4.15EE104 pKa = 3.84MRR106 pKa = 11.84IYY108 pKa = 10.8CPADD112 pKa = 3.02TDD114 pKa = 4.11FGRR117 pKa = 11.84DD118 pKa = 3.31EE119 pKa = 4.19VQKK122 pKa = 10.63LVRR125 pKa = 11.84NAIVRR130 pKa = 11.84ALKK133 pKa = 10.32RR134 pKa = 11.84RR135 pKa = 11.84AEE137 pKa = 4.22SFLPPLLAAWSARR150 pKa = 11.84CGLPYY155 pKa = 10.22RR156 pKa = 11.84KK157 pKa = 9.67VRR159 pKa = 11.84ITGARR164 pKa = 11.84TRR166 pKa = 11.84WGSCTATRR174 pKa = 11.84TISLSCYY181 pKa = 10.21LMLVPAHH188 pKa = 6.3LMDD191 pKa = 3.62YY192 pKa = 11.62VMLHH196 pKa = 6.16EE197 pKa = 4.7LAHH200 pKa = 5.42TRR202 pKa = 11.84EE203 pKa = 4.46MNHH206 pKa = 6.44GPRR209 pKa = 11.84FWEE212 pKa = 4.44LLDD215 pKa = 4.28SLTDD219 pKa = 4.0GQSHH223 pKa = 6.86RR224 pKa = 11.84LRR226 pKa = 11.84AEE228 pKa = 3.43LRR230 pKa = 11.84NFHH233 pKa = 6.36TGFF236 pKa = 3.81

Molecular weight: 27.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2561

0

2561

913119

29

2418

356.5

40.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.328 ± 0.047

1.296 ± 0.017

5.466 ± 0.035

6.748 ± 0.045

4.46 ± 0.03

6.955 ± 0.041

1.974 ± 0.021

6.539 ± 0.048

6.09 ± 0.038

9.42 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.774 ± 0.023

4.709 ± 0.04

3.908 ± 0.027

3.793 ± 0.028

4.803 ± 0.033

5.818 ± 0.036

5.708 ± 0.035

6.59 ± 0.036

1.264 ± 0.021

4.355 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski