Amino acid dipepetide frequency for Escherichia phage ZCEC5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.476AlaAla: 10.476 ± 1.785
1.376AlaCys: 1.376 ± 0.314
5.353AlaAsp: 5.353 ± 0.707
6.882AlaGlu: 6.882 ± 0.933
4.282AlaPhe: 4.282 ± 0.526
7.341AlaGly: 7.341 ± 0.734
1.912AlaHis: 1.912 ± 0.38
6.27AlaIle: 6.27 ± 0.901
5.123AlaLys: 5.123 ± 0.754
7.876AlaLeu: 7.876 ± 0.904
2.294AlaMet: 2.294 ± 0.45
3.517AlaAsn: 3.517 ± 0.643
3.747AlaPro: 3.747 ± 0.483
3.059AlaGln: 3.059 ± 0.588
4.511AlaArg: 4.511 ± 0.576
5.582AlaSer: 5.582 ± 0.789
6.499AlaThr: 6.499 ± 0.891
6.652AlaVal: 6.652 ± 0.774
1.529AlaTrp: 1.529 ± 0.284
3.364AlaTyr: 3.364 ± 0.418
0.0AlaXaa: 0.0 ± 0.0
Cys
1.071CysAla: 1.071 ± 0.347
0.229CysCys: 0.229 ± 0.136
1.071CysAsp: 1.071 ± 0.319
0.994CysGlu: 0.994 ± 0.4
0.229CysPhe: 0.229 ± 0.136
0.765CysGly: 0.765 ± 0.289
0.612CysHis: 0.612 ± 0.224
0.765CysIle: 0.765 ± 0.278
0.535CysLys: 0.535 ± 0.202
0.841CysLeu: 0.841 ± 0.299
0.306CysMet: 0.306 ± 0.12
0.382CysAsn: 0.382 ± 0.161
0.459CysPro: 0.459 ± 0.181
0.306CysGln: 0.306 ± 0.144
0.765CysArg: 0.765 ± 0.263
0.459CysSer: 0.459 ± 0.175
0.459CysThr: 0.459 ± 0.165
0.688CysVal: 0.688 ± 0.227
0.306CysTrp: 0.306 ± 0.174
0.306CysTyr: 0.306 ± 0.18
0.0CysXaa: 0.0 ± 0.0
Asp
6.499AspAla: 6.499 ± 0.685
0.612AspCys: 0.612 ± 0.203
4.511AspAsp: 4.511 ± 0.57
4.741AspGlu: 4.741 ± 0.645
2.447AspPhe: 2.447 ± 0.353
5.276AspGly: 5.276 ± 0.737
0.535AspHis: 0.535 ± 0.178
4.588AspIle: 4.588 ± 0.647
3.059AspLys: 3.059 ± 0.502
5.047AspLeu: 5.047 ± 0.618
1.606AspMet: 1.606 ± 0.273
2.141AspAsn: 2.141 ± 0.473
2.065AspPro: 2.065 ± 0.374
0.918AspGln: 0.918 ± 0.303
2.676AspArg: 2.676 ± 0.572
2.906AspSer: 2.906 ± 0.423
4.282AspThr: 4.282 ± 0.56
4.358AspVal: 4.358 ± 0.509
1.071AspTrp: 1.071 ± 0.231
2.523AspTyr: 2.523 ± 0.363
0.0AspXaa: 0.0 ± 0.0
Glu
5.888GluAla: 5.888 ± 0.871
0.612GluCys: 0.612 ± 0.292
4.206GluAsp: 4.206 ± 0.588
5.811GluGlu: 5.811 ± 1.051
3.135GluPhe: 3.135 ± 0.453
4.741GluGly: 4.741 ± 0.67
0.841GluHis: 0.841 ± 0.247
2.982GluIle: 2.982 ± 0.523
3.747GluLys: 3.747 ± 0.677
5.888GluLeu: 5.888 ± 0.567
3.364GluMet: 3.364 ± 0.476
3.212GluAsn: 3.212 ± 0.549
2.065GluPro: 2.065 ± 0.497
3.747GluGln: 3.747 ± 0.839
4.053GluArg: 4.053 ± 0.723
3.441GluSer: 3.441 ± 0.456
3.67GluThr: 3.67 ± 0.535
4.97GluVal: 4.97 ± 0.576
1.147GluTrp: 1.147 ± 0.305
2.37GluTyr: 2.37 ± 0.39
0.0GluXaa: 0.0 ± 0.0
Phe
2.829PheAla: 2.829 ± 0.441
0.535PheCys: 0.535 ± 0.22
3.364PheAsp: 3.364 ± 0.654
2.753PheGlu: 2.753 ± 0.548
0.994PhePhe: 0.994 ± 0.251
3.441PheGly: 3.441 ± 0.547
0.459PheHis: 0.459 ± 0.194
2.37PheIle: 2.37 ± 0.509
2.294PheLys: 2.294 ± 0.48
2.37PheLeu: 2.37 ± 0.466
0.382PheMet: 0.382 ± 0.15
1.453PheAsn: 1.453 ± 0.341
1.529PhePro: 1.529 ± 0.374
1.453PheGln: 1.453 ± 0.307
2.065PheArg: 2.065 ± 0.377
2.37PheSer: 2.37 ± 0.543
3.212PheThr: 3.212 ± 0.418
2.37PheVal: 2.37 ± 0.439
0.841PheTrp: 0.841 ± 0.303
1.529PheTyr: 1.529 ± 0.309
0.0PheXaa: 0.0 ± 0.0
Gly
6.958GlyAla: 6.958 ± 0.746
1.147GlyCys: 1.147 ± 0.325
4.358GlyAsp: 4.358 ± 0.586
5.276GlyGlu: 5.276 ± 0.775
3.517GlyPhe: 3.517 ± 0.6
5.888GlyGly: 5.888 ± 0.682
1.682GlyHis: 1.682 ± 0.369
2.676GlyIle: 2.676 ± 0.492
4.817GlyLys: 4.817 ± 0.666
4.97GlyLeu: 4.97 ± 0.595
2.294GlyMet: 2.294 ± 0.531
3.594GlyAsn: 3.594 ± 0.715
1.912GlyPro: 1.912 ± 0.396
3.288GlyGln: 3.288 ± 0.564
3.441GlyArg: 3.441 ± 0.494
5.658GlySer: 5.658 ± 0.824
4.741GlyThr: 4.741 ± 0.69
5.964GlyVal: 5.964 ± 0.676
1.147GlyTrp: 1.147 ± 0.261
2.829GlyTyr: 2.829 ± 0.492
0.0GlyXaa: 0.0 ± 0.0
His
1.529HisAla: 1.529 ± 0.41
0.306HisCys: 0.306 ± 0.157
0.994HisAsp: 0.994 ± 0.255
1.223HisGlu: 1.223 ± 0.357
0.765HisPhe: 0.765 ± 0.237
1.223HisGly: 1.223 ± 0.306
0.994HisHis: 0.994 ± 0.299
0.612HisIle: 0.612 ± 0.203
1.3HisLys: 1.3 ± 0.29
1.147HisLeu: 1.147 ± 0.281
0.153HisMet: 0.153 ± 0.106
0.688HisAsn: 0.688 ± 0.24
0.918HisPro: 0.918 ± 0.327
0.841HisGln: 0.841 ± 0.258
0.688HisArg: 0.688 ± 0.223
0.459HisSer: 0.459 ± 0.161
0.612HisThr: 0.612 ± 0.244
1.223HisVal: 1.223 ± 0.325
0.0HisTrp: 0.0 ± 0.0
0.535HisTyr: 0.535 ± 0.223
0.0HisXaa: 0.0 ± 0.0
Ile
5.429IleAla: 5.429 ± 0.774
0.688IleCys: 0.688 ± 0.192
3.823IleAsp: 3.823 ± 0.497
3.594IleGlu: 3.594 ± 0.412
1.071IlePhe: 1.071 ± 0.278
3.212IleGly: 3.212 ± 0.421
0.459IleHis: 0.459 ± 0.167
2.447IleIle: 2.447 ± 0.3
3.288IleLys: 3.288 ± 0.502
3.059IleLeu: 3.059 ± 0.447
0.994IleMet: 0.994 ± 0.321
3.135IleAsn: 3.135 ± 0.506
2.6IlePro: 2.6 ± 0.408
1.529IleGln: 1.529 ± 0.385
2.447IleArg: 2.447 ± 0.362
3.288IleSer: 3.288 ± 0.543
4.97IleThr: 4.97 ± 0.614
4.129IleVal: 4.129 ± 0.443
1.071IleTrp: 1.071 ± 0.294
1.682IleTyr: 1.682 ± 0.35
0.0IleXaa: 0.0 ± 0.0
Lys
6.499LysAla: 6.499 ± 1.13
0.306LysCys: 0.306 ± 0.164
3.212LysAsp: 3.212 ± 0.492
4.129LysGlu: 4.129 ± 0.834
2.065LysPhe: 2.065 ± 0.292
3.517LysGly: 3.517 ± 0.388
1.147LysHis: 1.147 ± 0.254
2.141LysIle: 2.141 ± 0.47
3.212LysLys: 3.212 ± 0.493
5.047LysLeu: 5.047 ± 0.598
2.676LysMet: 2.676 ± 0.675
1.606LysAsn: 1.606 ± 0.41
2.37LysPro: 2.37 ± 0.46
2.065LysGln: 2.065 ± 0.464
3.9LysArg: 3.9 ± 0.596
2.982LysSer: 2.982 ± 0.503
3.517LysThr: 3.517 ± 0.553
2.982LysVal: 2.982 ± 0.524
0.918LysTrp: 0.918 ± 0.199
2.906LysTyr: 2.906 ± 0.43
0.0LysXaa: 0.0 ± 0.0
Leu
8.87LeuAla: 8.87 ± 0.849
0.612LeuCys: 0.612 ± 0.263
4.129LeuAsp: 4.129 ± 0.624
4.97LeuGlu: 4.97 ± 0.72
2.523LeuPhe: 2.523 ± 0.594
5.429LeuGly: 5.429 ± 0.549
0.918LeuHis: 0.918 ± 0.264
4.741LeuIle: 4.741 ± 0.553
4.282LeuLys: 4.282 ± 0.585
4.817LeuLeu: 4.817 ± 0.645
1.912LeuMet: 1.912 ± 0.323
3.517LeuAsn: 3.517 ± 0.729
3.059LeuPro: 3.059 ± 0.509
3.059LeuGln: 3.059 ± 0.548
5.353LeuArg: 5.353 ± 0.69
3.594LeuSer: 3.594 ± 0.494
4.97LeuThr: 4.97 ± 0.683
4.588LeuVal: 4.588 ± 0.67
1.071LeuTrp: 1.071 ± 0.297
1.835LeuTyr: 1.835 ± 0.347
0.0LeuXaa: 0.0 ± 0.0
Met
2.6MetAla: 2.6 ± 0.377
0.535MetCys: 0.535 ± 0.177
0.918MetAsp: 0.918 ± 0.315
1.529MetGlu: 1.529 ± 0.374
0.918MetPhe: 0.918 ± 0.233
1.606MetGly: 1.606 ± 0.392
0.382MetHis: 0.382 ± 0.204
1.682MetIle: 1.682 ± 0.348
1.912MetLys: 1.912 ± 0.431
1.912MetLeu: 1.912 ± 0.338
0.382MetMet: 0.382 ± 0.189
0.994MetAsn: 0.994 ± 0.306
1.606MetPro: 1.606 ± 0.317
0.535MetGln: 0.535 ± 0.206
1.071MetArg: 1.071 ± 0.222
2.065MetSer: 2.065 ± 0.374
1.988MetThr: 1.988 ± 0.422
1.529MetVal: 1.529 ± 0.346
0.382MetTrp: 0.382 ± 0.176
0.688MetTyr: 0.688 ± 0.26
0.0MetXaa: 0.0 ± 0.0
Asn
3.823AsnAla: 3.823 ± 0.446
0.612AsnCys: 0.612 ± 0.254
2.447AsnAsp: 2.447 ± 0.468
2.6AsnGlu: 2.6 ± 0.415
1.071AsnPhe: 1.071 ± 0.254
4.129AsnGly: 4.129 ± 0.713
0.306AsnHis: 0.306 ± 0.161
1.988AsnIle: 1.988 ± 0.373
1.912AsnLys: 1.912 ± 0.349
2.753AsnLeu: 2.753 ± 0.391
0.841AsnMet: 0.841 ± 0.243
2.294AsnAsn: 2.294 ± 0.472
1.912AsnPro: 1.912 ± 0.362
1.912AsnGln: 1.912 ± 0.396
2.141AsnArg: 2.141 ± 0.329
2.523AsnSer: 2.523 ± 0.403
3.212AsnThr: 3.212 ± 0.726
3.364AsnVal: 3.364 ± 0.451
0.765AsnTrp: 0.765 ± 0.221
1.606AsnTyr: 1.606 ± 0.336
0.0AsnXaa: 0.0 ± 0.0
Pro
3.212ProAla: 3.212 ± 0.54
0.382ProCys: 0.382 ± 0.173
2.906ProAsp: 2.906 ± 0.434
4.282ProGlu: 4.282 ± 0.602
2.141ProPhe: 2.141 ± 0.435
2.906ProGly: 2.906 ± 0.473
0.459ProHis: 0.459 ± 0.15
2.065ProIle: 2.065 ± 0.427
1.453ProLys: 1.453 ± 0.444
3.059ProLeu: 3.059 ± 0.488
0.765ProMet: 0.765 ± 0.273
1.453ProAsn: 1.453 ± 0.327
0.994ProPro: 0.994 ± 0.314
0.918ProGln: 0.918 ± 0.241
1.835ProArg: 1.835 ± 0.383
2.217ProSer: 2.217 ± 0.384
1.912ProThr: 1.912 ± 0.35
4.206ProVal: 4.206 ± 0.593
0.306ProTrp: 0.306 ± 0.153
1.071ProTyr: 1.071 ± 0.311
0.0ProXaa: 0.0 ± 0.0
Gln
4.053GlnAla: 4.053 ± 0.692
0.535GlnCys: 0.535 ± 0.273
2.141GlnAsp: 2.141 ± 0.414
1.912GlnGlu: 1.912 ± 0.415
1.453GlnPhe: 1.453 ± 0.285
1.759GlnGly: 1.759 ± 0.338
0.688GlnHis: 0.688 ± 0.238
2.217GlnIle: 2.217 ± 0.337
2.676GlnLys: 2.676 ± 0.529
3.059GlnLeu: 3.059 ± 0.412
0.994GlnMet: 0.994 ± 0.317
1.453GlnAsn: 1.453 ± 0.394
1.682GlnPro: 1.682 ± 0.301
2.065GlnGln: 2.065 ± 0.639
2.523GlnArg: 2.523 ± 0.396
1.988GlnSer: 1.988 ± 0.455
2.141GlnThr: 2.141 ± 0.464
2.523GlnVal: 2.523 ± 0.435
0.994GlnTrp: 0.994 ± 0.244
1.453GlnTyr: 1.453 ± 0.34
0.0GlnXaa: 0.0 ± 0.0
Arg
3.9ArgAla: 3.9 ± 0.6
0.688ArgCys: 0.688 ± 0.241
2.829ArgAsp: 2.829 ± 0.46
4.206ArgGlu: 4.206 ± 0.617
1.606ArgPhe: 1.606 ± 0.281
4.511ArgGly: 4.511 ± 0.5
1.3ArgHis: 1.3 ± 0.277
3.135ArgIle: 3.135 ± 0.436
3.976ArgLys: 3.976 ± 0.609
3.67ArgLeu: 3.67 ± 0.56
1.147ArgMet: 1.147 ± 0.248
3.059ArgAsn: 3.059 ± 0.344
1.835ArgPro: 1.835 ± 0.376
2.676ArgGln: 2.676 ± 0.402
4.664ArgArg: 4.664 ± 0.704
2.906ArgSer: 2.906 ± 0.428
2.676ArgThr: 2.676 ± 0.391
3.976ArgVal: 3.976 ± 0.535
0.765ArgTrp: 0.765 ± 0.257
1.835ArgTyr: 1.835 ± 0.38
0.0ArgXaa: 0.0 ± 0.0
Ser
5.429SerAla: 5.429 ± 0.647
0.535SerCys: 0.535 ± 0.193
3.594SerAsp: 3.594 ± 0.517
3.135SerGlu: 3.135 ± 0.448
2.37SerPhe: 2.37 ± 0.452
6.041SerGly: 6.041 ± 0.763
0.765SerHis: 0.765 ± 0.222
2.6SerIle: 2.6 ± 0.71
3.364SerLys: 3.364 ± 0.615
4.588SerLeu: 4.588 ± 0.71
1.147SerMet: 1.147 ± 0.253
2.676SerAsn: 2.676 ± 0.431
1.988SerPro: 1.988 ± 0.355
2.447SerGln: 2.447 ± 0.609
2.982SerArg: 2.982 ± 0.54
3.059SerSer: 3.059 ± 0.496
3.517SerThr: 3.517 ± 0.543
4.664SerVal: 4.664 ± 0.522
0.765SerTrp: 0.765 ± 0.274
1.606SerTyr: 1.606 ± 0.363
0.0SerXaa: 0.0 ± 0.0
Thr
8.029ThrAla: 8.029 ± 1.052
0.382ThrCys: 0.382 ± 0.195
4.206ThrAsp: 4.206 ± 0.49
3.67ThrGlu: 3.67 ± 0.506
2.982ThrPhe: 2.982 ± 0.601
5.888ThrGly: 5.888 ± 0.99
0.918ThrHis: 0.918 ± 0.211
3.364ThrIle: 3.364 ± 0.489
3.67ThrLys: 3.67 ± 0.523
4.741ThrLeu: 4.741 ± 0.623
1.376ThrMet: 1.376 ± 0.308
1.376ThrAsn: 1.376 ± 0.407
4.511ThrPro: 4.511 ± 0.659
2.141ThrGln: 2.141 ± 0.358
2.753ThrArg: 2.753 ± 0.448
3.288ThrSer: 3.288 ± 0.506
4.206ThrThr: 4.206 ± 0.766
4.435ThrVal: 4.435 ± 0.591
0.688ThrTrp: 0.688 ± 0.242
2.294ThrTyr: 2.294 ± 0.487
0.0ThrXaa: 0.0 ± 0.0
Val
6.041ValAla: 6.041 ± 0.664
0.918ValCys: 0.918 ± 0.267
4.206ValAsp: 4.206 ± 0.467
5.123ValGlu: 5.123 ± 0.712
2.676ValPhe: 2.676 ± 0.494
4.435ValGly: 4.435 ± 0.546
0.918ValHis: 0.918 ± 0.258
4.358ValIle: 4.358 ± 0.468
3.517ValLys: 3.517 ± 0.543
4.97ValLeu: 4.97 ± 0.554
1.376ValMet: 1.376 ± 0.345
3.212ValAsn: 3.212 ± 0.458
1.912ValPro: 1.912 ± 0.384
3.059ValGln: 3.059 ± 0.47
4.588ValArg: 4.588 ± 0.608
5.353ValSer: 5.353 ± 0.644
5.353ValThr: 5.353 ± 0.641
5.811ValVal: 5.811 ± 0.945
0.994ValTrp: 0.994 ± 0.276
2.906ValTyr: 2.906 ± 0.535
0.0ValXaa: 0.0 ± 0.0
Trp
1.453TrpAla: 1.453 ± 0.455
0.076TrpCys: 0.076 ± 0.071
0.918TrpAsp: 0.918 ± 0.228
0.688TrpGlu: 0.688 ± 0.198
0.994TrpPhe: 0.994 ± 0.328
0.841TrpGly: 0.841 ± 0.304
0.229TrpHis: 0.229 ± 0.19
0.229TrpIle: 0.229 ± 0.207
0.994TrpLys: 0.994 ± 0.255
1.835TrpLeu: 1.835 ± 0.276
0.459TrpMet: 0.459 ± 0.161
0.688TrpAsn: 0.688 ± 0.247
0.306TrpPro: 0.306 ± 0.181
0.765TrpGln: 0.765 ± 0.251
1.071TrpArg: 1.071 ± 0.331
0.918TrpSer: 0.918 ± 0.282
0.765TrpThr: 0.765 ± 0.242
1.223TrpVal: 1.223 ± 0.354
0.306TrpTrp: 0.306 ± 0.209
0.535TrpTyr: 0.535 ± 0.208
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.059TyrAla: 3.059 ± 0.481
0.459TyrCys: 0.459 ± 0.145
2.676TyrAsp: 2.676 ± 0.517
2.37TyrGlu: 2.37 ± 0.421
1.376TyrPhe: 1.376 ± 0.382
3.212TyrGly: 3.212 ± 0.533
0.688TyrHis: 0.688 ± 0.214
1.682TyrIle: 1.682 ± 0.406
2.141TyrLys: 2.141 ± 0.362
2.6TyrLeu: 2.6 ± 0.387
0.688TyrMet: 0.688 ± 0.197
1.759TyrAsn: 1.759 ± 0.404
1.071TyrPro: 1.071 ± 0.316
1.529TyrGln: 1.529 ± 0.297
1.835TyrArg: 1.835 ± 0.355
2.217TyrSer: 2.217 ± 0.416
2.217TyrThr: 2.217 ± 0.506
2.065TyrVal: 2.065 ± 0.407
0.229TyrTrp: 0.229 ± 0.148
0.994TyrTyr: 0.994 ± 0.203
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 72 proteins (13079 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski