Amino acid dipepetide frequency for Mycobacterium phage Mowgli

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.91AlaAla: 19.91 ± 1.918
0.872AlaCys: 0.872 ± 0.231
8.502AlaAsp: 8.502 ± 0.741
7.92AlaGlu: 7.92 ± 0.871
4.069AlaPhe: 4.069 ± 0.682
14.896AlaGly: 14.896 ± 1.656
2.325AlaHis: 2.325 ± 0.601
6.394AlaIle: 6.394 ± 0.746
5.304AlaLys: 5.304 ± 0.773
9.592AlaLeu: 9.592 ± 0.78
3.343AlaMet: 3.343 ± 0.433
3.706AlaAsn: 3.706 ± 0.661
6.758AlaPro: 6.758 ± 1.186
4.796AlaGln: 4.796 ± 0.492
8.211AlaArg: 8.211 ± 0.894
5.45AlaSer: 5.45 ± 1.065
7.048AlaThr: 7.048 ± 0.699
8.502AlaVal: 8.502 ± 0.82
2.616AlaTrp: 2.616 ± 0.448
2.107AlaTyr: 2.107 ± 0.475
0.0AlaXaa: 0.0 ± 0.0
Cys
1.017CysAla: 1.017 ± 0.298
0.073CysCys: 0.073 ± 0.078
0.363CysAsp: 0.363 ± 0.182
0.436CysGlu: 0.436 ± 0.163
0.291CysPhe: 0.291 ± 0.14
1.744CysGly: 1.744 ± 0.464
0.073CysHis: 0.073 ± 0.079
0.291CysIle: 0.291 ± 0.125
0.291CysLys: 0.291 ± 0.125
0.363CysLeu: 0.363 ± 0.156
0.073CysMet: 0.073 ± 0.075
0.363CysAsn: 0.363 ± 0.175
0.727CysPro: 0.727 ± 0.313
0.581CysGln: 0.581 ± 0.215
1.017CysArg: 1.017 ± 0.242
0.509CysSer: 0.509 ± 0.231
0.581CysThr: 0.581 ± 0.299
0.581CysVal: 0.581 ± 0.197
0.291CysTrp: 0.291 ± 0.214
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
8.72AspAla: 8.72 ± 0.789
0.291AspCys: 0.291 ± 0.197
6.54AspAsp: 6.54 ± 0.905
5.377AspGlu: 5.377 ± 0.655
2.107AspPhe: 2.107 ± 0.45
7.848AspGly: 7.848 ± 0.864
1.235AspHis: 1.235 ± 0.309
1.599AspIle: 1.599 ± 0.329
1.817AspLys: 1.817 ± 0.484
5.086AspLeu: 5.086 ± 0.568
0.872AspMet: 0.872 ± 0.239
1.308AspAsn: 1.308 ± 0.298
4.65AspPro: 4.65 ± 0.837
2.979AspGln: 2.979 ± 0.573
4.36AspArg: 4.36 ± 0.642
2.325AspSer: 2.325 ± 0.391
3.415AspThr: 3.415 ± 0.48
5.086AspVal: 5.086 ± 0.804
1.235AspTrp: 1.235 ± 0.248
2.689AspTyr: 2.689 ± 0.467
0.0AspXaa: 0.0 ± 0.0
Glu
5.74GluAla: 5.74 ± 0.849
0.363GluCys: 0.363 ± 0.196
4.215GluAsp: 4.215 ± 0.605
3.343GluGlu: 3.343 ± 0.671
1.671GluPhe: 1.671 ± 0.375
3.997GluGly: 3.997 ± 0.52
2.471GluHis: 2.471 ± 0.473
2.18GluIle: 2.18 ± 0.41
1.235GluLys: 1.235 ± 0.245
6.394GluLeu: 6.394 ± 0.668
1.163GluMet: 1.163 ± 0.258
2.18GluAsn: 2.18 ± 0.508
3.851GluPro: 3.851 ± 0.823
2.18GluGln: 2.18 ± 0.396
4.142GluArg: 4.142 ± 0.784
2.253GluSer: 2.253 ± 0.44
2.979GluThr: 2.979 ± 0.505
3.851GluVal: 3.851 ± 0.399
1.163GluTrp: 1.163 ± 0.361
0.581GluTyr: 0.581 ± 0.198
0.0GluXaa: 0.0 ± 0.0
Phe
3.197PheAla: 3.197 ± 0.408
0.509PheCys: 0.509 ± 0.191
2.761PheAsp: 2.761 ± 0.392
2.398PheGlu: 2.398 ± 0.559
0.799PhePhe: 0.799 ± 0.225
3.706PheGly: 3.706 ± 0.65
0.436PheHis: 0.436 ± 0.172
0.654PheIle: 0.654 ± 0.222
0.727PheLys: 0.727 ± 0.206
2.253PheLeu: 2.253 ± 0.501
0.654PheMet: 0.654 ± 0.22
1.09PheAsn: 1.09 ± 0.353
1.235PhePro: 1.235 ± 0.309
0.727PheGln: 0.727 ± 0.201
1.453PheArg: 1.453 ± 0.436
2.398PheSer: 2.398 ± 0.433
1.381PheThr: 1.381 ± 0.265
2.035PheVal: 2.035 ± 0.355
0.436PheTrp: 0.436 ± 0.204
0.436PheTyr: 0.436 ± 0.179
0.0PheXaa: 0.0 ± 0.0
Gly
11.844GlyAla: 11.844 ± 0.86
0.799GlyCys: 0.799 ± 0.237
6.322GlyAsp: 6.322 ± 0.661
4.432GlyGlu: 4.432 ± 0.448
2.979GlyPhe: 2.979 ± 0.342
7.339GlyGly: 7.339 ± 1.344
2.398GlyHis: 2.398 ± 0.411
4.578GlyIle: 4.578 ± 0.67
4.505GlyLys: 4.505 ± 0.766
7.63GlyLeu: 7.63 ± 0.961
1.235GlyMet: 1.235 ± 0.22
2.979GlyAsn: 2.979 ± 0.533
3.924GlyPro: 3.924 ± 0.608
2.616GlyGln: 2.616 ± 0.556
5.886GlyArg: 5.886 ± 0.73
5.377GlySer: 5.377 ± 0.707
5.014GlyThr: 5.014 ± 0.723
6.104GlyVal: 6.104 ± 0.68
2.107GlyTrp: 2.107 ± 0.397
2.398GlyTyr: 2.398 ± 0.402
0.0GlyXaa: 0.0 ± 0.0
His
2.616HisAla: 2.616 ± 0.633
0.363HisCys: 0.363 ± 0.181
1.599HisAsp: 1.599 ± 0.341
1.235HisGlu: 1.235 ± 0.309
0.363HisPhe: 0.363 ± 0.187
1.744HisGly: 1.744 ± 0.332
0.509HisHis: 0.509 ± 0.216
1.09HisIle: 1.09 ± 0.268
0.654HisLys: 0.654 ± 0.221
1.235HisLeu: 1.235 ± 0.305
0.727HisMet: 0.727 ± 0.193
0.872HisAsn: 0.872 ± 0.233
2.18HisPro: 2.18 ± 0.485
0.872HisGln: 0.872 ± 0.241
2.543HisArg: 2.543 ± 0.555
0.872HisSer: 0.872 ± 0.2
0.872HisThr: 0.872 ± 0.257
1.671HisVal: 1.671 ± 0.349
0.581HisTrp: 0.581 ± 0.191
0.218HisTyr: 0.218 ± 0.124
0.0HisXaa: 0.0 ± 0.0
Ile
5.813IleAla: 5.813 ± 0.687
0.509IleCys: 0.509 ± 0.187
3.27IleAsp: 3.27 ± 0.476
3.197IleGlu: 3.197 ± 0.565
1.163IlePhe: 1.163 ± 0.198
4.142IleGly: 4.142 ± 0.701
0.945IleHis: 0.945 ± 0.261
1.017IleIle: 1.017 ± 0.329
1.308IleLys: 1.308 ± 0.52
2.834IleLeu: 2.834 ± 0.457
0.727IleMet: 0.727 ± 0.287
0.799IleAsn: 0.799 ± 0.301
4.36IlePro: 4.36 ± 0.667
1.453IleGln: 1.453 ± 0.258
3.779IleArg: 3.779 ± 0.479
1.599IleSer: 1.599 ± 0.286
1.962IleThr: 1.962 ± 0.593
2.907IleVal: 2.907 ± 0.468
1.09IleTrp: 1.09 ± 0.296
1.017IleTyr: 1.017 ± 0.266
0.0IleXaa: 0.0 ± 0.0
Lys
5.813LysAla: 5.813 ± 0.694
0.291LysCys: 0.291 ± 0.154
1.235LysAsp: 1.235 ± 0.377
0.727LysGlu: 0.727 ± 0.232
0.945LysPhe: 0.945 ± 0.284
1.962LysGly: 1.962 ± 0.489
0.727LysHis: 0.727 ± 0.268
2.18LysIle: 2.18 ± 0.524
1.526LysLys: 1.526 ± 0.383
2.616LysLeu: 2.616 ± 0.688
0.945LysMet: 0.945 ± 0.257
1.599LysAsn: 1.599 ± 0.326
2.834LysPro: 2.834 ± 0.472
1.163LysGln: 1.163 ± 0.341
2.616LysArg: 2.616 ± 0.512
1.962LysSer: 1.962 ± 0.341
2.834LysThr: 2.834 ± 0.519
2.107LysVal: 2.107 ± 0.347
0.363LysTrp: 0.363 ± 0.143
0.727LysTyr: 0.727 ± 0.211
0.0LysXaa: 0.0 ± 0.0
Leu
9.228LeuAla: 9.228 ± 0.818
0.509LeuCys: 0.509 ± 0.229
6.322LeuAsp: 6.322 ± 0.645
3.197LeuGlu: 3.197 ± 0.422
2.325LeuPhe: 2.325 ± 0.498
7.048LeuGly: 7.048 ± 0.936
1.599LeuHis: 1.599 ± 0.315
4.215LeuIle: 4.215 ± 0.635
2.907LeuLys: 2.907 ± 0.603
4.65LeuLeu: 4.65 ± 0.574
1.381LeuMet: 1.381 ± 0.329
1.889LeuAsn: 1.889 ± 0.386
4.941LeuPro: 4.941 ± 0.49
1.671LeuGln: 1.671 ± 0.418
5.232LeuArg: 5.232 ± 0.712
4.432LeuSer: 4.432 ± 0.615
5.304LeuThr: 5.304 ± 0.669
5.595LeuVal: 5.595 ± 0.771
1.09LeuTrp: 1.09 ± 0.403
1.599LeuTyr: 1.599 ± 0.407
0.0LeuXaa: 0.0 ± 0.0
Met
2.471MetAla: 2.471 ± 0.421
0.145MetCys: 0.145 ± 0.108
0.872MetAsp: 0.872 ± 0.253
0.799MetGlu: 0.799 ± 0.198
0.872MetPhe: 0.872 ± 0.223
1.235MetGly: 1.235 ± 0.274
0.872MetHis: 0.872 ± 0.329
1.017MetIle: 1.017 ± 0.282
0.799MetLys: 0.799 ± 0.232
2.107MetLeu: 2.107 ± 0.368
0.363MetMet: 0.363 ± 0.159
0.581MetAsn: 0.581 ± 0.188
0.581MetPro: 0.581 ± 0.231
0.872MetGln: 0.872 ± 0.23
1.235MetArg: 1.235 ± 0.281
1.671MetSer: 1.671 ± 0.373
2.18MetThr: 2.18 ± 0.332
1.163MetVal: 1.163 ± 0.28
0.291MetTrp: 0.291 ± 0.139
0.218MetTyr: 0.218 ± 0.117
0.0MetXaa: 0.0 ± 0.0
Asn
4.796AsnAla: 4.796 ± 0.705
0.581AsnCys: 0.581 ± 0.251
0.945AsnAsp: 0.945 ± 0.335
1.526AsnGlu: 1.526 ± 0.311
0.872AsnPhe: 0.872 ± 0.293
3.052AsnGly: 3.052 ± 0.454
0.654AsnHis: 0.654 ± 0.219
1.381AsnIle: 1.381 ± 0.373
0.654AsnLys: 0.654 ± 0.272
1.962AsnLeu: 1.962 ± 0.347
0.363AsnMet: 0.363 ± 0.189
0.581AsnAsn: 0.581 ± 0.211
2.979AsnPro: 2.979 ± 0.415
1.308AsnGln: 1.308 ± 0.344
2.253AsnArg: 2.253 ± 0.357
0.799AsnSer: 0.799 ± 0.19
2.035AsnThr: 2.035 ± 0.358
1.817AsnVal: 1.817 ± 0.617
0.291AsnTrp: 0.291 ± 0.115
0.727AsnTyr: 0.727 ± 0.226
0.0AsnXaa: 0.0 ± 0.0
Pro
11.481ProAla: 11.481 ± 1.229
0.509ProCys: 0.509 ± 0.261
4.723ProAsp: 4.723 ± 0.768
4.287ProGlu: 4.287 ± 0.632
1.308ProPhe: 1.308 ± 0.321
4.941ProGly: 4.941 ± 0.78
1.453ProHis: 1.453 ± 0.37
2.543ProIle: 2.543 ± 0.444
2.18ProLys: 2.18 ± 0.412
3.924ProLeu: 3.924 ± 0.543
0.799ProMet: 0.799 ± 0.295
1.526ProAsn: 1.526 ± 0.387
3.706ProPro: 3.706 ± 0.587
2.107ProGln: 2.107 ± 0.542
3.706ProArg: 3.706 ± 0.667
3.125ProSer: 3.125 ± 0.368
3.851ProThr: 3.851 ± 0.656
3.488ProVal: 3.488 ± 0.514
0.945ProTrp: 0.945 ± 0.303
0.945ProTyr: 0.945 ± 0.239
0.0ProXaa: 0.0 ± 0.0
Gln
4.505GlnAla: 4.505 ± 0.656
0.291GlnCys: 0.291 ± 0.14
2.543GlnAsp: 2.543 ± 0.371
1.235GlnGlu: 1.235 ± 0.275
1.453GlnPhe: 1.453 ± 0.356
2.471GlnGly: 2.471 ± 0.427
1.308GlnHis: 1.308 ± 0.368
1.962GlnIle: 1.962 ± 0.437
1.235GlnLys: 1.235 ± 0.28
3.197GlnLeu: 3.197 ± 0.534
0.872GlnMet: 0.872 ± 0.209
1.308GlnAsn: 1.308 ± 0.476
1.671GlnPro: 1.671 ± 0.31
1.817GlnGln: 1.817 ± 0.444
2.689GlnArg: 2.689 ± 0.471
1.599GlnSer: 1.599 ± 0.414
1.453GlnThr: 1.453 ± 0.289
1.962GlnVal: 1.962 ± 0.463
0.581GlnTrp: 0.581 ± 0.195
0.727GlnTyr: 0.727 ± 0.232
0.0GlnXaa: 0.0 ± 0.0
Arg
9.156ArgAla: 9.156 ± 1.101
1.163ArgCys: 1.163 ± 0.359
4.432ArgAsp: 4.432 ± 0.405
3.343ArgGlu: 3.343 ± 0.484
1.599ArgPhe: 1.599 ± 0.365
4.65ArgGly: 4.65 ± 0.649
1.308ArgHis: 1.308 ± 0.359
3.27ArgIle: 3.27 ± 0.428
2.689ArgLys: 2.689 ± 0.506
4.941ArgLeu: 4.941 ± 0.598
2.107ArgMet: 2.107 ± 0.455
2.907ArgAsn: 2.907 ± 0.498
4.432ArgPro: 4.432 ± 0.582
2.907ArgGln: 2.907 ± 0.428
7.121ArgArg: 7.121 ± 1.126
3.125ArgSer: 3.125 ± 0.491
4.578ArgThr: 4.578 ± 0.514
3.779ArgVal: 3.779 ± 0.554
1.526ArgTrp: 1.526 ± 0.372
1.526ArgTyr: 1.526 ± 0.315
0.0ArgXaa: 0.0 ± 0.0
Ser
5.813SerAla: 5.813 ± 1.037
0.654SerCys: 0.654 ± 0.259
4.142SerAsp: 4.142 ± 0.526
3.125SerGlu: 3.125 ± 0.6
1.526SerPhe: 1.526 ± 0.324
4.723SerGly: 4.723 ± 1.029
0.509SerHis: 0.509 ± 0.186
2.325SerIle: 2.325 ± 0.418
2.325SerLys: 2.325 ± 0.455
3.343SerLeu: 3.343 ± 0.546
1.381SerMet: 1.381 ± 0.286
1.526SerAsn: 1.526 ± 0.402
2.689SerPro: 2.689 ± 0.489
1.09SerGln: 1.09 ± 0.271
3.488SerArg: 3.488 ± 0.524
3.706SerSer: 3.706 ± 0.66
3.779SerThr: 3.779 ± 0.642
3.27SerVal: 3.27 ± 0.536
1.235SerTrp: 1.235 ± 0.331
1.163SerTyr: 1.163 ± 0.299
0.0SerXaa: 0.0 ± 0.0
Thr
8.138ThrAla: 8.138 ± 0.99
0.509ThrCys: 0.509 ± 0.208
3.633ThrAsp: 3.633 ± 0.453
2.834ThrGlu: 2.834 ± 0.436
1.962ThrPhe: 1.962 ± 0.401
5.668ThrGly: 5.668 ± 0.842
1.308ThrHis: 1.308 ± 0.278
2.616ThrIle: 2.616 ± 0.51
1.817ThrLys: 1.817 ± 0.319
3.706ThrLeu: 3.706 ± 0.614
1.599ThrMet: 1.599 ± 0.341
1.526ThrAsn: 1.526 ± 0.342
4.287ThrPro: 4.287 ± 0.699
1.453ThrGln: 1.453 ± 0.305
3.633ThrArg: 3.633 ± 0.597
4.36ThrSer: 4.36 ± 0.672
4.36ThrThr: 4.36 ± 0.643
5.014ThrVal: 5.014 ± 0.502
0.727ThrTrp: 0.727 ± 0.247
1.017ThrTyr: 1.017 ± 0.323
0.0ThrXaa: 0.0 ± 0.0
Val
7.92ValAla: 7.92 ± 0.86
0.945ValCys: 0.945 ± 0.312
5.232ValAsp: 5.232 ± 0.68
4.868ValGlu: 4.868 ± 0.532
2.18ValPhe: 2.18 ± 0.39
5.232ValGly: 5.232 ± 0.585
1.453ValHis: 1.453 ± 0.233
2.616ValIle: 2.616 ± 0.431
1.889ValLys: 1.889 ± 0.319
5.014ValLeu: 5.014 ± 0.548
0.945ValMet: 0.945 ± 0.278
2.035ValAsn: 2.035 ± 0.457
3.706ValPro: 3.706 ± 0.453
2.18ValGln: 2.18 ± 0.407
4.36ValArg: 4.36 ± 0.64
3.415ValSer: 3.415 ± 0.695
4.432ValThr: 4.432 ± 0.536
5.304ValVal: 5.304 ± 0.772
2.035ValTrp: 2.035 ± 0.474
1.381ValTyr: 1.381 ± 0.269
0.0ValXaa: 0.0 ± 0.0
Trp
2.035TrpAla: 2.035 ± 0.377
0.145TrpCys: 0.145 ± 0.099
0.945TrpAsp: 0.945 ± 0.239
0.654TrpGlu: 0.654 ± 0.231
0.436TrpPhe: 0.436 ± 0.165
1.744TrpGly: 1.744 ± 0.38
0.581TrpHis: 0.581 ± 0.188
1.163TrpIle: 1.163 ± 0.296
0.799TrpLys: 0.799 ± 0.295
2.325TrpLeu: 2.325 ± 0.563
0.509TrpMet: 0.509 ± 0.205
0.509TrpAsn: 0.509 ± 0.17
1.017TrpPro: 1.017 ± 0.239
0.945TrpGln: 0.945 ± 0.226
1.453TrpArg: 1.453 ± 0.33
1.671TrpSer: 1.671 ± 0.388
0.945TrpThr: 0.945 ± 0.253
0.872TrpVal: 0.872 ± 0.211
0.291TrpTrp: 0.291 ± 0.127
0.436TrpTyr: 0.436 ± 0.195
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.107TyrAla: 2.107 ± 0.469
0.145TyrCys: 0.145 ± 0.095
0.945TyrAsp: 0.945 ± 0.269
1.017TyrGlu: 1.017 ± 0.282
0.363TyrPhe: 0.363 ± 0.18
2.325TyrGly: 2.325 ± 0.37
0.654TyrHis: 0.654 ± 0.251
0.799TyrIle: 0.799 ± 0.249
0.509TyrLys: 0.509 ± 0.198
1.962TyrLeu: 1.962 ± 0.354
0.218TyrMet: 0.218 ± 0.156
0.291TyrAsn: 0.291 ± 0.141
1.017TyrPro: 1.017 ± 0.288
1.163TyrGln: 1.163 ± 0.309
1.453TyrArg: 1.453 ± 0.272
1.163TyrSer: 1.163 ± 0.339
1.163TyrThr: 1.163 ± 0.33
2.035TyrVal: 2.035 ± 0.368
0.581TyrTrp: 0.581 ± 0.174
0.363TyrTyr: 0.363 ± 0.174
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (13763 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski