Streptococcus phage CHPC925

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Moineauvirus; unclassified Moineauvirus

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8F7R1|A0A3G8F7R1_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC925 OX=2365047 GN=CHPC925_0026 PE=4 SV=1
MM1 pKa = 7.11VNWVDD6 pKa = 4.52ADD8 pKa = 4.28GNDD11 pKa = 4.07LPDD14 pKa = 5.09GADD17 pKa = 3.18QDD19 pKa = 4.63FKK21 pKa = 11.75AGMFFSFAGDD31 pKa = 3.45EE32 pKa = 4.32VNITDD37 pKa = 4.04TGTGGYY43 pKa = 9.64YY44 pKa = 10.32GGYY47 pKa = 7.71YY48 pKa = 8.88WRR50 pKa = 11.84LFEE53 pKa = 4.82FGQFGTVWLSCWNKK67 pKa = 10.66DD68 pKa = 3.89DD69 pKa = 5.14LVNYY73 pKa = 7.38YY74 pKa = 8.26QQ75 pKa = 4.22

Molecular weight: 8.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8F7X5|A0A3G8F7X5_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC925 OX=2365047 GN=CHPC925_0018 PE=4 SV=1
MM1 pKa = 7.36ARR3 pKa = 11.84VRR5 pKa = 11.84YY6 pKa = 9.32LPSDD10 pKa = 3.79FRR12 pKa = 11.84YY13 pKa = 9.99KK14 pKa = 10.95ADD16 pKa = 3.48FGTYY20 pKa = 9.38QSTPNKK26 pKa = 8.16FTGVSVPKK34 pKa = 10.14FVKK37 pKa = 10.38QFTLHH42 pKa = 5.9YY43 pKa = 9.16KK44 pKa = 9.46PHH46 pKa = 6.1TRR48 pKa = 11.84TLNQEE53 pKa = 3.83YY54 pKa = 10.01LAQQNGEE61 pKa = 3.75SDD63 pKa = 3.27TRR65 pKa = 11.84VIVIRR70 pKa = 11.84HH71 pKa = 4.59NVKK74 pKa = 10.47VIEE77 pKa = 4.53GQAVVLNGTQYY88 pKa = 11.37DD89 pKa = 3.79IVRR92 pKa = 11.84VSSNEE97 pKa = 3.55NFGLNRR103 pKa = 11.84YY104 pKa = 9.75DD105 pKa = 3.94YY106 pKa = 10.41LTLKK110 pKa = 9.73KK111 pKa = 9.68HH112 pKa = 5.98KK113 pKa = 10.48KK114 pKa = 9.32IGG116 pKa = 3.46

Molecular weight: 13.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

10387

39

1603

230.8

26.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.633 ± 0.509

0.568 ± 0.127

6.412 ± 0.279

6.826 ± 0.531

4.246 ± 0.197

6.576 ± 0.471

1.54 ± 0.121

6.624 ± 0.211

8.636 ± 0.505

7.644 ± 0.389

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.628 ± 0.164

6.508 ± 0.274

2.821 ± 0.212

4.669 ± 0.24

4.169 ± 0.311

6.142 ± 0.284

6.094 ± 0.312

5.94 ± 0.204

1.454 ± 0.141

3.87 ± 0.283
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski