Microcystis phage vB_MaeS-yong1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G8Z7W5|A0A7G8Z7W5_9CAUD DNA-binding-regulatory protein OS=Microcystis phage vB_MaeS-yong1 OX=2762278 PE=4 SV=1
MM1 pKa = 7.11QPIGPEE7 pKa = 3.94SKK9 pKa = 10.61EE10 pKa = 3.85PGLAAICVLAMCMVATAATPAVCAGLPQSANEE42 pKa = 4.21GAGEE46 pKa = 3.83IHH48 pKa = 6.58EE49 pKa = 4.8CEE51 pKa = 4.13NN52 pKa = 3.63

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G8Z7T5|A0A7G8Z7T5_9CAUD Uncharacterized protein OS=Microcystis phage vB_MaeS-yong1 OX=2762278 PE=4 SV=1
MM1 pKa = 7.54SRR3 pKa = 11.84EE4 pKa = 3.9LSRR7 pKa = 11.84NRR9 pKa = 11.84CAEE12 pKa = 3.8AEE14 pKa = 4.32AYY16 pKa = 9.97SSRR19 pKa = 11.84AAGSGYY25 pKa = 8.31RR26 pKa = 11.84QRR28 pKa = 11.84RR29 pKa = 11.84QRR31 pKa = 11.84SVRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84LIEE40 pKa = 3.66GTPLYY45 pKa = 10.29RR46 pKa = 11.84RR47 pKa = 11.84VYY49 pKa = 8.73AWLVYY54 pKa = 10.14RR55 pKa = 11.84RR56 pKa = 11.84WSPQQIAARR65 pKa = 11.84LRR67 pKa = 11.84HH68 pKa = 5.58MHH70 pKa = 7.16PDD72 pKa = 3.39DD73 pKa = 4.06PAWRR77 pKa = 11.84VSHH80 pKa = 5.23EE81 pKa = 4.14TVYY84 pKa = 11.09AAIYY88 pKa = 8.01AHH90 pKa = 6.92PRR92 pKa = 11.84GALKK96 pKa = 10.54KK97 pKa = 10.8GMVDD101 pKa = 3.99ALRR104 pKa = 11.84QSKK107 pKa = 8.04PQRR110 pKa = 11.84GRR112 pKa = 11.84RR113 pKa = 11.84RR114 pKa = 11.84TTAAGASFVPEE125 pKa = 3.92AQRR128 pKa = 11.84IVHH131 pKa = 6.29RR132 pKa = 11.84PEE134 pKa = 4.98EE135 pKa = 4.31IDD137 pKa = 3.35TRR139 pKa = 11.84LLPGHH144 pKa = 6.64WEE146 pKa = 3.76GDD148 pKa = 4.2FIKK151 pKa = 10.67GAYY154 pKa = 8.35NRR156 pKa = 11.84SGVGTLVEE164 pKa = 4.14RR165 pKa = 11.84KK166 pKa = 7.21TRR168 pKa = 11.84FVVLCKK174 pKa = 9.93MEE176 pKa = 4.37GCTAQAALEE185 pKa = 4.4GFTRR189 pKa = 11.84QLKK192 pKa = 9.99KK193 pKa = 10.73LPSFLRR199 pKa = 11.84EE200 pKa = 3.72SLTYY204 pKa = 10.7DD205 pKa = 3.13RR206 pKa = 11.84GSEE209 pKa = 4.21MACHH213 pKa = 6.63AEE215 pKa = 3.66LSEE218 pKa = 4.26RR219 pKa = 11.84LNLEE223 pKa = 3.17IWFADD228 pKa = 3.54PHH230 pKa = 6.76APWQRR235 pKa = 11.84GSNEE239 pKa = 3.8NTNGLLRR246 pKa = 11.84QFLPKK251 pKa = 10.06GTDD254 pKa = 3.22LSQVSQTHH262 pKa = 6.2LNDD265 pKa = 3.22IARR268 pKa = 11.84LLNGRR273 pKa = 11.84PRR275 pKa = 11.84QTLGWLTPEE284 pKa = 3.5EE285 pKa = 4.11MMAKK289 pKa = 10.04EE290 pKa = 3.73MAEE293 pKa = 3.89FAKK296 pKa = 10.34RR297 pKa = 11.84VALDD301 pKa = 3.26SS302 pKa = 3.96

Molecular weight: 34.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

13491

37

2073

240.9

26.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.801 ± 1.104

1.001 ± 0.169

5.329 ± 0.279

6.805 ± 0.243

2.906 ± 0.124

7.894 ± 0.359

1.757 ± 0.291

3.995 ± 0.172

2.913 ± 0.352

9.925 ± 0.316

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.157 ± 0.125

2.535 ± 0.138

5.144 ± 0.53

4.788 ± 0.396

8.576 ± 0.409

5.73 ± 0.227

5.433 ± 0.263

6.612 ± 0.323

1.616 ± 0.205

2.083 ± 0.174
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski