Ferriphaselus amnicola

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Gallionellaceae; Ferriphaselus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2668 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z6GBS4|A0A2Z6GBS4_9PROT Uncharacterized protein OS=Ferriphaselus amnicola OX=1188319 GN=OYT1_ch1391 PE=4 SV=1
MM1 pKa = 7.63GDD3 pKa = 3.33MVFAAHH9 pKa = 7.3DD10 pKa = 4.41LYY12 pKa = 11.49NEE14 pKa = 4.17SLEE17 pKa = 4.29EE18 pKa = 4.09TGEE21 pKa = 4.03SSIPGVEE28 pKa = 3.91PDD30 pKa = 4.36ALLAAAGTRR39 pKa = 11.84GVVVNVGHH47 pKa = 6.01AQEE50 pKa = 4.51MPNEE54 pKa = 4.26EE55 pKa = 4.09IYY57 pKa = 10.84LVRR60 pKa = 11.84FEE62 pKa = 4.67IDD64 pKa = 2.83ADD66 pKa = 3.85GTLAEE71 pKa = 5.37PIGCLSDD78 pKa = 3.58EE79 pKa = 4.58LSGLNN84 pKa = 3.86

Molecular weight: 8.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z6GBY6|A0A2Z6GBY6_9PROT Polysaccharide export protein OS=Ferriphaselus amnicola OX=1188319 GN=OYT1_ch1464 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 10.08RR3 pKa = 11.84ISQAGFLKK11 pKa = 10.34PFRR14 pKa = 11.84RR15 pKa = 11.84FLRR18 pKa = 11.84TILQSRR24 pKa = 11.84FVRR27 pKa = 11.84TGNKK31 pKa = 9.72KK32 pKa = 10.1PGLVKK37 pKa = 10.89ANAGLL42 pKa = 3.57

Molecular weight: 4.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2668

0

2668

828448

30

2834

310.5

34.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.744 ± 0.053

0.966 ± 0.016

5.24 ± 0.03

6.017 ± 0.048

3.681 ± 0.032

7.459 ± 0.05

2.417 ± 0.022

5.366 ± 0.037

4.258 ± 0.046

10.923 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.625 ± 0.026

3.29 ± 0.031

4.363 ± 0.035

4.259 ± 0.034

5.998 ± 0.045

6.007 ± 0.037

5.158 ± 0.041

7.338 ± 0.048

1.296 ± 0.019

2.595 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski