Erwinia phage vB_EamP-S2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Molineuxvirinae; Eracentumvirus; Erwinia virus S2

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9V4Y2|A0A2K9V4Y2_9CAUD Hypotheticla protein OS=Erwinia phage vB_EamP-S2 OX=2070198 PE=4 SV=1
MM1 pKa = 7.46LNEE4 pKa = 4.33LSIDD8 pKa = 3.75LDD10 pKa = 3.95HH11 pKa = 7.85PILTSAGRR19 pKa = 11.84AAKK22 pKa = 10.02QVDD25 pKa = 4.13VTEE28 pKa = 4.41MLLQKK33 pKa = 9.43LTAGFEE39 pKa = 4.23PGPEE43 pKa = 4.05LLKK46 pKa = 10.74QAADD50 pKa = 3.92LGIDD54 pKa = 3.33ICAIRR59 pKa = 11.84AMVNEE64 pKa = 5.05LYY66 pKa = 11.16GEE68 pKa = 4.77DD69 pKa = 4.16EE70 pKa = 4.85DD71 pKa = 5.72DD72 pKa = 3.68

Molecular weight: 7.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K9V4W2|A0A2K9V4W2_9CAUD Hypotheticla protein OS=Erwinia phage vB_EamP-S2 OX=2070198 PE=4 SV=1
MM1 pKa = 6.71MTLKK5 pKa = 10.27EE6 pKa = 4.12YY7 pKa = 11.0KK8 pKa = 8.98KK9 pKa = 8.12TLRR12 pKa = 11.84YY13 pKa = 9.51AVGYY17 pKa = 9.04SASLATLKK25 pKa = 10.68RR26 pKa = 11.84IARR29 pKa = 11.84NSKK32 pKa = 9.61VKK34 pKa = 10.42HH35 pKa = 5.33LPARR39 pKa = 11.84SKK41 pKa = 11.08LRR43 pKa = 11.84IAKK46 pKa = 10.05RR47 pKa = 11.84ARR49 pKa = 11.84SQDD52 pKa = 2.95KK53 pKa = 9.86MIAFAHH59 pKa = 6.85RR60 pKa = 11.84YY61 pKa = 8.42SAASTPRR68 pKa = 11.84TSRR71 pKa = 11.84QVYY74 pKa = 10.52DD75 pKa = 4.06GFIQQAADD83 pKa = 3.89FSNIEE88 pKa = 4.06LKK90 pKa = 10.54ILASHH95 pKa = 6.49TDD97 pKa = 3.04INDD100 pKa = 3.31SYY102 pKa = 11.94LLIDD106 pKa = 3.78

Molecular weight: 12.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

13824

42

1291

282.1

31.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.172 ± 0.475

0.81 ± 0.144

6.453 ± 0.227

6.467 ± 0.337

3.508 ± 0.17

7.928 ± 0.328

1.982 ± 0.228

5.064 ± 0.205

5.924 ± 0.264

7.827 ± 0.29

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.161 ± 0.137

4.34 ± 0.256

3.812 ± 0.212

4.333 ± 0.294

5.628 ± 0.259

6.366 ± 0.331

5.584 ± 0.323

6.836 ± 0.306

1.44 ± 0.128

3.364 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski