Pseudozyma hubeiensis (strain SY62) (Yeast)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina; Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Pseudozyma; Pseudozyma hubeiensis

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7472 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9NYD4|R9NYD4_PSEHS Uncharacterized protein OS=Pseudozyma hubeiensis (strain SY62) OX=1305764 GN=PHSY_001343 PE=3 SV=1
MM1 pKa = 7.36PVSYY5 pKa = 10.66YY6 pKa = 10.97DD7 pKa = 3.95EE8 pKa = 6.02IEE10 pKa = 4.57LEE12 pKa = 4.1DD13 pKa = 3.49MSYY16 pKa = 11.37DD17 pKa = 3.61EE18 pKa = 5.29EE19 pKa = 5.88KK20 pKa = 11.17DD21 pKa = 3.56LFHH24 pKa = 6.71YY25 pKa = 7.97PCPCGDD31 pKa = 3.15RR32 pKa = 11.84FEE34 pKa = 4.45ITRR37 pKa = 11.84QQLKK41 pKa = 9.38EE42 pKa = 4.1AEE44 pKa = 4.2DD45 pKa = 3.75VARR48 pKa = 11.84CPSCSLIIRR57 pKa = 11.84VVFDD61 pKa = 5.15PIDD64 pKa = 3.96FEE66 pKa = 7.44DD67 pKa = 5.68DD68 pKa = 3.55DD69 pKa = 6.8DD70 pKa = 4.23EE71 pKa = 6.92APVNTIDD78 pKa = 4.34VASQTVAAAAA88 pKa = 3.78

Molecular weight: 10.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9P9U4|R9P9U4_PSEHS Uncharacterized protein OS=Pseudozyma hubeiensis (strain SY62) OX=1305764 GN=PHSY_005735 PE=4 SV=1
MM1 pKa = 6.98STEE4 pKa = 3.9KK5 pKa = 10.45PGRR8 pKa = 11.84TKK10 pKa = 10.18MLEE13 pKa = 3.85RR14 pKa = 11.84PRR16 pKa = 11.84GKK18 pKa = 10.42GLSLTVRR25 pKa = 11.84RR26 pKa = 11.84KK27 pKa = 9.8PMRR30 pKa = 11.84NYY32 pKa = 11.4ALTCPIWTAMMLDD45 pKa = 3.62RR46 pKa = 11.84EE47 pKa = 4.56MGSSEE52 pKa = 4.0ASRR55 pKa = 11.84PIAILAFPSPEE66 pKa = 3.73RR67 pKa = 11.84HH68 pKa = 6.36SPLCSPHH75 pKa = 5.71QHH77 pKa = 5.94TPSPSLLPNHH87 pKa = 6.5CRR89 pKa = 11.84ASSPPILFSHH99 pKa = 6.8VARR102 pKa = 11.84TYY104 pKa = 10.97NQLHH108 pKa = 5.44PQGRR112 pKa = 11.84NQKK115 pKa = 9.21VLARR119 pKa = 11.84SQLHH123 pKa = 5.98RR124 pKa = 11.84RR125 pKa = 11.84RR126 pKa = 11.84QIRR129 pKa = 11.84SSRR132 pKa = 11.84RR133 pKa = 11.84NRR135 pKa = 11.84QVSLLLPALRR145 pKa = 11.84FALLSEE151 pKa = 4.49IKK153 pKa = 9.78TLTYY157 pKa = 10.71SDD159 pKa = 5.27AIASLEE165 pKa = 4.08CCRR168 pKa = 11.84NGNKK172 pKa = 9.68FYY174 pKa = 10.92EE175 pKa = 4.28NHH177 pKa = 6.09NEE179 pKa = 3.53FNLRR183 pKa = 11.84HH184 pKa = 5.83RR185 pKa = 11.84WVDD188 pKa = 3.38YY189 pKa = 11.0AADD192 pKa = 3.72NEE194 pKa = 4.57FNASQVDD201 pKa = 4.14PLWHH205 pKa = 6.27SWLHH209 pKa = 6.51HH210 pKa = 5.6IRR212 pKa = 11.84KK213 pKa = 9.68DD214 pKa = 3.69PPHH217 pKa = 7.11EE218 pKa = 4.26DD219 pKa = 2.89AGIIKK224 pKa = 7.57MTQSWMTVSAAPRR237 pKa = 3.45

Molecular weight: 27.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7472

0

7472

4147995

66

5397

555.1

60.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.351 ± 0.032

1.066 ± 0.011

5.9 ± 0.023

5.604 ± 0.026

3.272 ± 0.015

6.8 ± 0.022

2.472 ± 0.012

4.038 ± 0.017

4.455 ± 0.024

8.734 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.935 ± 0.01

3.195 ± 0.013

5.988 ± 0.025

4.245 ± 0.022

6.697 ± 0.025

10.092 ± 0.04

5.901 ± 0.016

5.888 ± 0.019

1.161 ± 0.009

2.2 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski