Amino acid dipepetide frequency for Escherichia phage vojen

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.965AlaAla: 6.965 ± 0.846
0.995AlaCys: 0.995 ± 0.296
4.511AlaAsp: 4.511 ± 0.604
5.108AlaGlu: 5.108 ± 0.548
2.852AlaPhe: 2.852 ± 0.516
4.776AlaGly: 4.776 ± 0.611
1.459AlaHis: 1.459 ± 0.266
5.837AlaIle: 5.837 ± 0.711
6.766AlaLys: 6.766 ± 0.791
6.567AlaLeu: 6.567 ± 0.675
3.184AlaMet: 3.184 ± 0.462
3.516AlaAsn: 3.516 ± 0.502
1.924AlaPro: 1.924 ± 0.332
3.317AlaGln: 3.317 ± 0.607
4.444AlaArg: 4.444 ± 0.552
4.909AlaSer: 4.909 ± 0.503
4.113AlaThr: 4.113 ± 0.73
5.97AlaVal: 5.97 ± 0.764
1.061AlaTrp: 1.061 ± 0.253
2.521AlaTyr: 2.521 ± 0.428
0.0AlaXaa: 0.0 ± 0.0
Cys
1.061CysAla: 1.061 ± 0.294
0.265CysCys: 0.265 ± 0.181
1.658CysAsp: 1.658 ± 0.282
0.929CysGlu: 0.929 ± 0.209
0.265CysPhe: 0.265 ± 0.13
1.658CysGly: 1.658 ± 0.405
0.265CysHis: 0.265 ± 0.114
0.796CysIle: 0.796 ± 0.208
0.929CysLys: 0.929 ± 0.231
0.73CysLeu: 0.73 ± 0.203
0.663CysMet: 0.663 ± 0.196
0.531CysAsn: 0.531 ± 0.191
0.398CysPro: 0.398 ± 0.129
0.265CysGln: 0.265 ± 0.122
0.73CysArg: 0.73 ± 0.302
0.398CysSer: 0.398 ± 0.152
0.73CysThr: 0.73 ± 0.2
0.929CysVal: 0.929 ± 0.255
0.398CysTrp: 0.398 ± 0.136
0.398CysTyr: 0.398 ± 0.152
0.0CysXaa: 0.0 ± 0.0
Asp
5.174AspAla: 5.174 ± 0.606
0.73AspCys: 0.73 ± 0.203
4.179AspAsp: 4.179 ± 0.602
4.643AspGlu: 4.643 ± 0.482
2.985AspPhe: 2.985 ± 0.419
6.633AspGly: 6.633 ± 0.78
1.128AspHis: 1.128 ± 0.284
3.317AspIle: 3.317 ± 0.451
3.847AspLys: 3.847 ± 0.462
4.378AspLeu: 4.378 ± 0.565
1.99AspMet: 1.99 ± 0.31
2.322AspAsn: 2.322 ± 0.336
2.587AspPro: 2.587 ± 0.334
2.056AspGln: 2.056 ± 0.359
2.72AspArg: 2.72 ± 0.406
3.516AspSer: 3.516 ± 0.476
3.383AspThr: 3.383 ± 0.611
3.582AspVal: 3.582 ± 0.43
1.061AspTrp: 1.061 ± 0.285
2.587AspTyr: 2.587 ± 0.415
0.0AspXaa: 0.0 ± 0.0
Glu
5.771GluAla: 5.771 ± 0.704
0.862GluCys: 0.862 ± 0.249
3.184GluAsp: 3.184 ± 0.42
4.577GluGlu: 4.577 ± 0.524
3.516GluPhe: 3.516 ± 0.483
3.184GluGly: 3.184 ± 0.378
0.995GluHis: 0.995 ± 0.248
5.837GluIle: 5.837 ± 0.624
4.312GluLys: 4.312 ± 0.535
4.909GluLeu: 4.909 ± 0.708
2.521GluMet: 2.521 ± 0.34
3.383GluAsn: 3.383 ± 0.442
1.857GluPro: 1.857 ± 0.411
2.985GluGln: 2.985 ± 0.543
3.051GluArg: 3.051 ± 0.605
4.378GluSer: 4.378 ± 0.483
3.648GluThr: 3.648 ± 0.504
4.643GluVal: 4.643 ± 0.55
0.73GluTrp: 0.73 ± 0.199
3.118GluTyr: 3.118 ± 0.446
0.0GluXaa: 0.0 ± 0.0
Phe
2.919PheAla: 2.919 ± 0.378
0.332PheCys: 0.332 ± 0.158
3.118PheAsp: 3.118 ± 0.454
2.388PheGlu: 2.388 ± 0.282
1.26PhePhe: 1.26 ± 0.318
3.184PheGly: 3.184 ± 0.503
0.73PheHis: 0.73 ± 0.224
2.653PheIle: 2.653 ± 0.362
3.25PheLys: 3.25 ± 0.44
1.857PheLeu: 1.857 ± 0.378
1.26PheMet: 1.26 ± 0.253
2.322PheAsn: 2.322 ± 0.338
1.393PhePro: 1.393 ± 0.31
1.26PheGln: 1.26 ± 0.279
2.255PheArg: 2.255 ± 0.382
1.924PheSer: 1.924 ± 0.356
2.587PheThr: 2.587 ± 0.389
2.388PheVal: 2.388 ± 0.442
0.796PheTrp: 0.796 ± 0.195
0.929PheTyr: 0.929 ± 0.355
0.0PheXaa: 0.0 ± 0.0
Gly
4.776GlyAla: 4.776 ± 0.746
1.459GlyCys: 1.459 ± 0.281
5.174GlyAsp: 5.174 ± 0.612
5.041GlyGlu: 5.041 ± 0.503
3.317GlyPhe: 3.317 ± 0.448
6.567GlyGly: 6.567 ± 1.081
1.26GlyHis: 1.26 ± 0.339
3.914GlyIle: 3.914 ± 0.478
6.7GlyLys: 6.7 ± 0.581
5.174GlyLeu: 5.174 ± 0.452
2.72GlyMet: 2.72 ± 0.4
3.914GlyAsn: 3.914 ± 0.486
0.464GlyPro: 0.464 ± 0.172
1.924GlyGln: 1.924 ± 0.308
2.852GlyArg: 2.852 ± 0.407
5.837GlySer: 5.837 ± 0.604
3.25GlyThr: 3.25 ± 0.447
6.103GlyVal: 6.103 ± 0.546
1.459GlyTrp: 1.459 ± 0.305
3.781GlyTyr: 3.781 ± 0.455
0.0GlyXaa: 0.0 ± 0.0
His
0.796HisAla: 0.796 ± 0.278
0.265HisCys: 0.265 ± 0.133
1.393HisAsp: 1.393 ± 0.309
1.061HisGlu: 1.061 ± 0.313
0.796HisPhe: 0.796 ± 0.234
1.791HisGly: 1.791 ± 0.364
0.464HisHis: 0.464 ± 0.235
1.194HisIle: 1.194 ± 0.275
1.526HisLys: 1.526 ± 0.342
1.26HisLeu: 1.26 ± 0.309
0.398HisMet: 0.398 ± 0.167
0.73HisAsn: 0.73 ± 0.266
0.597HisPro: 0.597 ± 0.213
0.73HisGln: 0.73 ± 0.247
0.995HisArg: 0.995 ± 0.319
0.796HisSer: 0.796 ± 0.236
0.995HisThr: 0.995 ± 0.239
1.26HisVal: 1.26 ± 0.286
0.133HisTrp: 0.133 ± 0.091
1.459HisTyr: 1.459 ± 0.316
0.0HisXaa: 0.0 ± 0.0
Ile
5.97IleAla: 5.97 ± 0.622
1.061IleCys: 1.061 ± 0.227
5.174IleAsp: 5.174 ± 0.547
4.909IleGlu: 4.909 ± 0.554
2.189IlePhe: 2.189 ± 0.373
4.909IleGly: 4.909 ± 0.48
1.658IleHis: 1.658 ± 0.355
3.715IleIle: 3.715 ± 0.478
4.842IleLys: 4.842 ± 0.63
2.521IleLeu: 2.521 ± 0.345
1.924IleMet: 1.924 ± 0.384
2.852IleAsn: 2.852 ± 0.557
2.123IlePro: 2.123 ± 0.353
2.653IleGln: 2.653 ± 0.601
3.25IleArg: 3.25 ± 0.482
3.914IleSer: 3.914 ± 0.523
4.245IleThr: 4.245 ± 0.457
4.378IleVal: 4.378 ± 0.501
1.061IleTrp: 1.061 ± 0.277
2.587IleTyr: 2.587 ± 0.436
0.0IleXaa: 0.0 ± 0.0
Lys
6.501LysAla: 6.501 ± 0.981
1.061LysCys: 1.061 ± 0.26
4.577LysAsp: 4.577 ± 0.51
5.638LysGlu: 5.638 ± 0.707
2.985LysPhe: 2.985 ± 0.42
4.113LysGly: 4.113 ± 0.57
1.592LysHis: 1.592 ± 0.377
3.648LysIle: 3.648 ± 0.489
4.378LysLys: 4.378 ± 0.529
5.373LysLeu: 5.373 ± 0.653
3.648LysMet: 3.648 ± 0.451
3.184LysAsn: 3.184 ± 0.618
3.847LysPro: 3.847 ± 0.508
3.317LysGln: 3.317 ± 0.496
4.312LysArg: 4.312 ± 0.595
3.98LysSer: 3.98 ± 0.417
4.245LysThr: 4.245 ± 0.54
4.975LysVal: 4.975 ± 0.515
0.995LysTrp: 0.995 ± 0.248
1.725LysTyr: 1.725 ± 0.371
0.0LysXaa: 0.0 ± 0.0
Leu
6.103LeuAla: 6.103 ± 0.595
0.73LeuCys: 0.73 ± 0.235
3.383LeuAsp: 3.383 ± 0.478
4.046LeuGlu: 4.046 ± 0.635
1.791LeuPhe: 1.791 ± 0.379
4.113LeuGly: 4.113 ± 0.662
1.393LeuHis: 1.393 ± 0.295
5.041LeuIle: 5.041 ± 0.559
5.373LeuLys: 5.373 ± 0.608
4.046LeuLeu: 4.046 ± 0.529
1.99LeuMet: 1.99 ± 0.351
3.781LeuAsn: 3.781 ± 0.467
2.919LeuPro: 2.919 ± 0.422
1.791LeuGln: 1.791 ± 0.329
3.847LeuArg: 3.847 ± 0.486
4.179LeuSer: 4.179 ± 0.513
4.113LeuThr: 4.113 ± 0.493
3.914LeuVal: 3.914 ± 0.443
0.929LeuTrp: 0.929 ± 0.263
2.255LeuTyr: 2.255 ± 0.292
0.0LeuXaa: 0.0 ± 0.0
Met
3.847MetAla: 3.847 ± 0.448
0.332MetCys: 0.332 ± 0.136
1.327MetAsp: 1.327 ± 0.339
1.99MetGlu: 1.99 ± 0.429
0.929MetPhe: 0.929 ± 0.259
1.393MetGly: 1.393 ± 0.298
0.663MetHis: 0.663 ± 0.222
2.919MetIle: 2.919 ± 0.444
2.72MetLys: 2.72 ± 0.397
2.454MetLeu: 2.454 ± 0.388
0.929MetMet: 0.929 ± 0.292
1.128MetAsn: 1.128 ± 0.29
1.061MetPro: 1.061 ± 0.254
1.459MetGln: 1.459 ± 0.245
1.924MetArg: 1.924 ± 0.258
2.189MetSer: 2.189 ± 0.325
2.056MetThr: 2.056 ± 0.361
1.857MetVal: 1.857 ± 0.276
0.199MetTrp: 0.199 ± 0.135
0.995MetTyr: 0.995 ± 0.198
0.0MetXaa: 0.0 ± 0.0
Asn
4.312AsnAla: 4.312 ± 0.512
0.663AsnCys: 0.663 ± 0.249
2.786AsnAsp: 2.786 ± 0.427
2.985AsnGlu: 2.985 ± 0.414
1.857AsnPhe: 1.857 ± 0.316
5.439AsnGly: 5.439 ± 0.592
0.73AsnHis: 0.73 ± 0.208
3.118AsnIle: 3.118 ± 0.416
3.648AsnLys: 3.648 ± 0.425
2.587AsnLeu: 2.587 ± 0.469
1.061AsnMet: 1.061 ± 0.281
2.521AsnAsn: 2.521 ± 0.402
1.526AsnPro: 1.526 ± 0.288
2.123AsnGln: 2.123 ± 0.328
1.725AsnArg: 1.725 ± 0.306
2.454AsnSer: 2.454 ± 0.388
1.393AsnThr: 1.393 ± 0.272
2.985AsnVal: 2.985 ± 0.346
0.796AsnTrp: 0.796 ± 0.194
1.924AsnTyr: 1.924 ± 0.36
0.0AsnXaa: 0.0 ± 0.0
Pro
1.99ProAla: 1.99 ± 0.408
0.597ProCys: 0.597 ± 0.204
2.587ProAsp: 2.587 ± 0.5
3.317ProGlu: 3.317 ± 0.58
1.725ProPhe: 1.725 ± 0.331
2.852ProGly: 2.852 ± 0.483
0.531ProHis: 0.531 ± 0.173
1.857ProIle: 1.857 ± 0.404
1.393ProLys: 1.393 ± 0.239
1.99ProLeu: 1.99 ± 0.407
0.995ProMet: 0.995 ± 0.256
1.924ProAsn: 1.924 ± 0.38
1.26ProPro: 1.26 ± 0.322
1.26ProGln: 1.26 ± 0.296
1.791ProArg: 1.791 ± 0.392
1.393ProSer: 1.393 ± 0.326
1.924ProThr: 1.924 ± 0.286
2.653ProVal: 2.653 ± 0.477
0.597ProTrp: 0.597 ± 0.18
1.658ProTyr: 1.658 ± 0.295
0.0ProXaa: 0.0 ± 0.0
Gln
2.985GlnAla: 2.985 ± 0.429
0.597GlnCys: 0.597 ± 0.184
1.526GlnAsp: 1.526 ± 0.297
2.322GlnGlu: 2.322 ± 0.392
1.393GlnPhe: 1.393 ± 0.301
2.322GlnGly: 2.322 ± 0.352
0.796GlnHis: 0.796 ± 0.229
2.786GlnIle: 2.786 ± 0.536
2.189GlnLys: 2.189 ± 0.383
2.985GlnLeu: 2.985 ± 0.519
0.995GlnMet: 0.995 ± 0.308
1.592GlnAsn: 1.592 ± 0.331
1.26GlnPro: 1.26 ± 0.356
2.852GlnGln: 2.852 ± 0.729
2.255GlnArg: 2.255 ± 0.336
2.388GlnSer: 2.388 ± 0.378
2.123GlnThr: 2.123 ± 0.438
3.051GlnVal: 3.051 ± 0.443
0.597GlnTrp: 0.597 ± 0.197
1.791GlnTyr: 1.791 ± 0.341
0.0GlnXaa: 0.0 ± 0.0
Arg
3.582ArgAla: 3.582 ± 0.545
0.929ArgCys: 0.929 ± 0.271
2.72ArgAsp: 2.72 ± 0.521
3.383ArgGlu: 3.383 ± 0.363
1.526ArgPhe: 1.526 ± 0.229
2.786ArgGly: 2.786 ± 0.384
0.597ArgHis: 0.597 ± 0.209
3.98ArgIle: 3.98 ± 0.42
4.444ArgLys: 4.444 ± 0.496
2.653ArgLeu: 2.653 ± 0.47
1.526ArgMet: 1.526 ± 0.302
1.99ArgAsn: 1.99 ± 0.495
1.99ArgPro: 1.99 ± 0.397
2.056ArgGln: 2.056 ± 0.418
3.383ArgArg: 3.383 ± 0.524
2.454ArgSer: 2.454 ± 0.414
1.857ArgThr: 1.857 ± 0.301
4.909ArgVal: 4.909 ± 0.793
0.597ArgTrp: 0.597 ± 0.208
2.521ArgTyr: 2.521 ± 0.422
0.0ArgXaa: 0.0 ± 0.0
Ser
5.373SerAla: 5.373 ± 0.566
0.597SerCys: 0.597 ± 0.195
3.715SerAsp: 3.715 ± 0.505
4.577SerGlu: 4.577 ± 0.533
2.521SerPhe: 2.521 ± 0.419
5.439SerGly: 5.439 ± 0.513
1.061SerHis: 1.061 ± 0.262
4.444SerIle: 4.444 ± 0.633
4.046SerLys: 4.046 ± 0.507
4.046SerLeu: 4.046 ± 0.456
1.061SerMet: 1.061 ± 0.217
2.189SerAsn: 2.189 ± 0.464
2.72SerPro: 2.72 ± 0.479
2.123SerGln: 2.123 ± 0.42
2.653SerArg: 2.653 ± 0.384
3.118SerSer: 3.118 ± 0.567
2.786SerThr: 2.786 ± 0.449
4.046SerVal: 4.046 ± 0.46
0.995SerTrp: 0.995 ± 0.269
2.322SerTyr: 2.322 ± 0.409
0.0SerXaa: 0.0 ± 0.0
Thr
4.643ThrAla: 4.643 ± 0.788
0.663ThrCys: 0.663 ± 0.252
3.184ThrAsp: 3.184 ± 0.571
2.653ThrGlu: 2.653 ± 0.443
2.322ThrPhe: 2.322 ± 0.322
6.036ThrGly: 6.036 ± 0.693
0.332ThrHis: 0.332 ± 0.155
3.184ThrIle: 3.184 ± 0.504
3.449ThrLys: 3.449 ± 0.616
3.98ThrLeu: 3.98 ± 0.468
1.26ThrMet: 1.26 ± 0.261
3.184ThrAsn: 3.184 ± 0.456
2.255ThrPro: 2.255 ± 0.32
2.388ThrGln: 2.388 ± 0.325
1.592ThrArg: 1.592 ± 0.321
3.781ThrSer: 3.781 ± 0.455
2.123ThrThr: 2.123 ± 0.362
3.383ThrVal: 3.383 ± 0.36
0.796ThrTrp: 0.796 ± 0.202
1.99ThrTyr: 1.99 ± 0.382
0.0ThrXaa: 0.0 ± 0.0
Val
4.511ValAla: 4.511 ± 0.608
1.061ValCys: 1.061 ± 0.239
4.975ValAsp: 4.975 ± 0.473
4.643ValGlu: 4.643 ± 0.609
2.653ValPhe: 2.653 ± 0.392
4.113ValGly: 4.113 ± 0.483
1.194ValHis: 1.194 ± 0.339
4.776ValIle: 4.776 ± 0.501
6.302ValLys: 6.302 ± 0.756
4.577ValLeu: 4.577 ± 0.438
2.388ValMet: 2.388 ± 0.361
3.25ValAsn: 3.25 ± 0.423
1.99ValPro: 1.99 ± 0.52
2.056ValGln: 2.056 ± 0.348
3.184ValArg: 3.184 ± 0.439
4.643ValSer: 4.643 ± 0.674
4.511ValThr: 4.511 ± 0.571
4.113ValVal: 4.113 ± 0.579
0.929ValTrp: 0.929 ± 0.206
2.123ValTyr: 2.123 ± 0.305
0.0ValXaa: 0.0 ± 0.0
Trp
0.796TrpAla: 0.796 ± 0.218
0.199TrpCys: 0.199 ± 0.122
0.929TrpAsp: 0.929 ± 0.224
0.597TrpGlu: 0.597 ± 0.163
0.796TrpPhe: 0.796 ± 0.28
1.327TrpGly: 1.327 ± 0.29
0.796TrpHis: 0.796 ± 0.184
0.929TrpIle: 0.929 ± 0.223
0.929TrpLys: 0.929 ± 0.218
1.393TrpLeu: 1.393 ± 0.278
0.398TrpMet: 0.398 ± 0.136
0.597TrpAsn: 0.597 ± 0.21
0.398TrpPro: 0.398 ± 0.157
0.398TrpGln: 0.398 ± 0.155
0.995TrpArg: 0.995 ± 0.25
0.929TrpSer: 0.929 ± 0.236
0.663TrpThr: 0.663 ± 0.199
0.73TrpVal: 0.73 ± 0.201
0.133TrpTrp: 0.133 ± 0.09
0.597TrpTyr: 0.597 ± 0.153
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.72TyrAla: 2.72 ± 0.493
0.597TyrCys: 0.597 ± 0.194
2.72TyrAsp: 2.72 ± 0.429
2.653TyrGlu: 2.653 ± 0.476
1.128TyrPhe: 1.128 ± 0.284
3.051TyrGly: 3.051 ± 0.434
0.995TyrHis: 0.995 ± 0.223
1.99TyrIle: 1.99 ± 0.422
3.051TyrLys: 3.051 ± 0.451
2.255TyrLeu: 2.255 ± 0.41
1.393TyrMet: 1.393 ± 0.34
1.725TyrAsn: 1.725 ± 0.298
1.725TyrPro: 1.725 ± 0.348
1.791TyrGln: 1.791 ± 0.302
1.924TyrArg: 1.924 ± 0.363
2.653TyrSer: 2.653 ± 0.418
2.454TyrThr: 2.454 ± 0.324
2.189TyrVal: 2.189 ± 0.317
0.265TyrTrp: 0.265 ± 0.115
1.194TyrTyr: 1.194 ± 0.258
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 80 proteins (15076 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski