Amino acid dipepetide frequency for Clostridium sp. MSTE9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.227AlaAla: 10.227 ± 0.162
1.311AlaCys: 1.311 ± 0.037
4.474AlaAsp: 4.474 ± 0.074
5.952AlaGlu: 5.952 ± 0.087
3.479AlaPhe: 3.479 ± 0.062
7.156AlaGly: 7.156 ± 0.099
1.285AlaHis: 1.285 ± 0.037
5.336AlaIle: 5.336 ± 0.08
4.547AlaLys: 4.547 ± 0.07
9.062AlaLeu: 9.062 ± 0.124
2.584AlaMet: 2.584 ± 0.062
2.6AlaAsn: 2.6 ± 0.052
2.962AlaPro: 2.962 ± 0.067
3.539AlaGln: 3.539 ± 0.078
3.96AlaArg: 3.96 ± 0.072
4.697AlaSer: 4.697 ± 0.075
3.445AlaThr: 3.445 ± 0.066
7.478AlaVal: 7.478 ± 0.099
0.653AlaTrp: 0.653 ± 0.026
2.546AlaTyr: 2.546 ± 0.053
0.0AlaXaa: 0.0 ± 0.0
Cys
1.369CysAla: 1.369 ± 0.037
0.298CysCys: 0.298 ± 0.019
0.887CysAsp: 0.887 ± 0.033
0.873CysGlu: 0.873 ± 0.033
0.638CysPhe: 0.638 ± 0.025
1.606CysGly: 1.606 ± 0.045
0.305CysHis: 0.305 ± 0.018
0.893CysIle: 0.893 ± 0.03
0.623CysLys: 0.623 ± 0.023
1.162CysLeu: 1.162 ± 0.035
0.382CysMet: 0.382 ± 0.02
0.444CysAsn: 0.444 ± 0.021
0.705CysPro: 0.705 ± 0.026
0.399CysGln: 0.399 ± 0.022
0.915CysArg: 0.915 ± 0.028
0.961CysSer: 0.961 ± 0.031
0.751CysThr: 0.751 ± 0.028
1.016CysVal: 1.016 ± 0.032
0.109CysTrp: 0.109 ± 0.01
0.482CysTyr: 0.482 ± 0.026
0.0CysXaa: 0.0 ± 0.0
Asp
4.243AspAla: 4.243 ± 0.065
0.78AspCys: 0.78 ± 0.032
2.374AspAsp: 2.374 ± 0.058
3.571AspGlu: 3.571 ± 0.059
2.439AspPhe: 2.439 ± 0.05
3.961AspGly: 3.961 ± 0.067
0.931AspHis: 0.931 ± 0.035
3.5AspIle: 3.5 ± 0.068
2.455AspLys: 2.455 ± 0.057
4.771AspLeu: 4.771 ± 0.074
1.426AspMet: 1.426 ± 0.036
1.601AspAsn: 1.601 ± 0.041
2.149AspPro: 2.149 ± 0.052
1.675AspGln: 1.675 ± 0.042
2.551AspArg: 2.551 ± 0.059
3.161AspSer: 3.161 ± 0.065
2.814AspThr: 2.814 ± 0.063
3.448AspVal: 3.448 ± 0.065
0.53AspTrp: 0.53 ± 0.027
2.07AspTyr: 2.07 ± 0.052
0.0AspXaa: 0.0 ± 0.0
Glu
5.102GluAla: 5.102 ± 0.071
0.694GluCys: 0.694 ± 0.025
3.105GluAsp: 3.105 ± 0.057
5.692GluGlu: 5.692 ± 0.093
2.249GluPhe: 2.249 ± 0.054
3.793GluGly: 3.793 ± 0.059
1.326GluHis: 1.326 ± 0.034
4.831GluIle: 4.831 ± 0.074
4.85GluLys: 4.85 ± 0.082
6.842GluLeu: 6.842 ± 0.1
2.13GluMet: 2.13 ± 0.048
3.321GluAsn: 3.321 ± 0.06
2.182GluPro: 2.182 ± 0.051
3.582GluGln: 3.582 ± 0.073
3.661GluArg: 3.661 ± 0.075
3.605GluSer: 3.605 ± 0.06
3.214GluThr: 3.214 ± 0.053
3.796GluVal: 3.796 ± 0.064
0.582GluTrp: 0.582 ± 0.026
2.42GluTyr: 2.42 ± 0.053
0.0GluXaa: 0.0 ± 0.0
Phe
3.424PheAla: 3.424 ± 0.065
0.822PheCys: 0.822 ± 0.028
2.328PheAsp: 2.328 ± 0.047
2.472PheGlu: 2.472 ± 0.059
2.015PhePhe: 2.015 ± 0.053
3.332PheGly: 3.332 ± 0.065
0.915PheHis: 0.915 ± 0.03
2.341PheIle: 2.341 ± 0.063
1.351PheLys: 1.351 ± 0.042
4.489PheLeu: 4.489 ± 0.097
0.952PheMet: 0.952 ± 0.029
1.253PheAsn: 1.253 ± 0.035
1.615PhePro: 1.615 ± 0.038
1.587PheGln: 1.587 ± 0.043
1.968PheArg: 1.968 ± 0.046
3.416PheSer: 3.416 ± 0.067
2.353PheThr: 2.353 ± 0.049
2.82PheVal: 2.82 ± 0.063
0.418PheTrp: 0.418 ± 0.022
1.486PheTyr: 1.486 ± 0.052
0.0PheXaa: 0.0 ± 0.0
Gly
6.349GlyAla: 6.349 ± 0.097
1.315GlyCys: 1.315 ± 0.044
3.455GlyAsp: 3.455 ± 0.066
4.59GlyGlu: 4.59 ± 0.074
3.338GlyPhe: 3.338 ± 0.065
5.871GlyGly: 5.871 ± 0.11
1.269GlyHis: 1.269 ± 0.036
6.033GlyIle: 6.033 ± 0.09
4.741GlyLys: 4.741 ± 0.071
6.804GlyLeu: 6.804 ± 0.097
2.559GlyMet: 2.559 ± 0.051
2.732GlyAsn: 2.732 ± 0.069
1.882GlyPro: 1.882 ± 0.064
2.436GlyGln: 2.436 ± 0.055
3.573GlyArg: 3.573 ± 0.068
4.519GlySer: 4.519 ± 0.08
4.363GlyThr: 4.363 ± 0.073
5.696GlyVal: 5.696 ± 0.086
0.74GlyTrp: 0.74 ± 0.03
2.842GlyTyr: 2.842 ± 0.052
0.0GlyXaa: 0.0 ± 0.0
His
1.322HisAla: 1.322 ± 0.037
0.333HisCys: 0.333 ± 0.02
0.846HisAsp: 0.846 ± 0.029
0.965HisGlu: 0.965 ± 0.028
0.919HisPhe: 0.919 ± 0.034
1.389HisGly: 1.389 ± 0.035
0.426HisHis: 0.426 ± 0.025
1.183HisIle: 1.183 ± 0.032
0.785HisLys: 0.785 ± 0.03
1.724HisLeu: 1.724 ± 0.042
0.495HisMet: 0.495 ± 0.02
0.657HisAsn: 0.657 ± 0.025
1.091HisPro: 1.091 ± 0.036
0.663HisGln: 0.663 ± 0.024
0.945HisArg: 0.945 ± 0.029
1.124HisSer: 1.124 ± 0.03
1.043HisThr: 1.043 ± 0.033
1.151HisVal: 1.151 ± 0.034
0.191HisTrp: 0.191 ± 0.014
0.659HisTyr: 0.659 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
6.068IleAla: 6.068 ± 0.088
1.132IleCys: 1.132 ± 0.032
3.588IleAsp: 3.588 ± 0.066
3.897IleGlu: 3.897 ± 0.064
2.59IlePhe: 2.59 ± 0.061
5.014IleGly: 5.014 ± 0.073
1.294IleHis: 1.294 ± 0.036
3.983IleIle: 3.983 ± 0.089
2.835IleLys: 2.835 ± 0.061
6.996IleLeu: 6.996 ± 0.106
1.608IleMet: 1.608 ± 0.044
2.32IleAsn: 2.32 ± 0.054
3.331IlePro: 3.331 ± 0.063
2.356IleGln: 2.356 ± 0.044
3.638IleArg: 3.638 ± 0.059
4.933IleSer: 4.933 ± 0.073
3.948IleThr: 3.948 ± 0.07
4.644IleVal: 4.644 ± 0.076
0.535IleTrp: 0.535 ± 0.026
2.026IleTyr: 2.026 ± 0.051
0.0IleXaa: 0.0 ± 0.0
Lys
4.622LysAla: 4.622 ± 0.067
0.5LysCys: 0.5 ± 0.023
2.737LysAsp: 2.737 ± 0.063
4.486LysGlu: 4.486 ± 0.077
1.382LysPhe: 1.382 ± 0.039
3.482LysGly: 3.482 ± 0.061
0.879LysHis: 0.879 ± 0.029
3.864LysIle: 3.864 ± 0.072
4.097LysLys: 4.097 ± 0.086
4.531LysLeu: 4.531 ± 0.07
1.78LysMet: 1.78 ± 0.042
2.697LysAsn: 2.697 ± 0.054
1.944LysPro: 1.944 ± 0.048
2.07LysGln: 2.07 ± 0.048
2.65LysArg: 2.65 ± 0.058
3.206LysSer: 3.206 ± 0.06
3.344LysThr: 3.344 ± 0.067
3.154LysVal: 3.154 ± 0.064
0.427LysTrp: 0.427 ± 0.021
1.845LysTyr: 1.845 ± 0.049
0.0LysXaa: 0.0 ± 0.0
Leu
8.281LeuAla: 8.281 ± 0.106
1.743LeuCys: 1.743 ± 0.042
4.887LeuAsp: 4.887 ± 0.074
6.046LeuGlu: 6.046 ± 0.085
4.43LeuPhe: 4.43 ± 0.091
6.773LeuGly: 6.773 ± 0.097
1.741LeuHis: 1.741 ± 0.05
6.274LeuIle: 6.274 ± 0.102
5.142LeuLys: 5.142 ± 0.083
10.542LeuLeu: 10.542 ± 0.137
2.823LeuMet: 2.823 ± 0.057
3.718LeuAsn: 3.718 ± 0.066
4.53LeuPro: 4.53 ± 0.079
3.365LeuGln: 3.365 ± 0.059
5.189LeuArg: 5.189 ± 0.08
7.579LeuSer: 7.579 ± 0.089
5.644LeuThr: 5.644 ± 0.077
6.223LeuVal: 6.223 ± 0.088
0.895LeuTrp: 0.895 ± 0.032
3.204LeuTyr: 3.204 ± 0.064
0.0LeuXaa: 0.0 ± 0.0
Met
2.542MetAla: 2.542 ± 0.049
0.301MetCys: 0.301 ± 0.017
1.498MetAsp: 1.498 ± 0.038
2.055MetGlu: 2.055 ± 0.054
0.946MetPhe: 0.946 ± 0.031
2.162MetGly: 2.162 ± 0.054
0.39MetHis: 0.39 ± 0.021
1.986MetIle: 1.986 ± 0.046
2.118MetLys: 2.118 ± 0.051
2.841MetLeu: 2.841 ± 0.057
0.891MetMet: 0.891 ± 0.037
1.363MetAsn: 1.363 ± 0.035
1.154MetPro: 1.154 ± 0.035
1.152MetGln: 1.152 ± 0.037
1.379MetArg: 1.379 ± 0.041
1.657MetSer: 1.657 ± 0.042
1.74MetThr: 1.74 ± 0.044
1.94MetVal: 1.94 ± 0.045
0.189MetTrp: 0.189 ± 0.013
0.692MetTyr: 0.692 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
3.078AsnAla: 3.078 ± 0.06
0.565AsnCys: 0.565 ± 0.025
1.76AsnAsp: 1.76 ± 0.046
2.046AsnGlu: 2.046 ± 0.055
1.351AsnPhe: 1.351 ± 0.036
3.169AsnGly: 3.169 ± 0.06
0.736AsnHis: 0.736 ± 0.028
2.619AsnIle: 2.619 ± 0.046
1.652AsnLys: 1.652 ± 0.042
3.543AsnLeu: 3.543 ± 0.064
1.06AsnMet: 1.06 ± 0.029
1.343AsnAsn: 1.343 ± 0.043
2.118AsnPro: 2.118 ± 0.047
1.524AsnGln: 1.524 ± 0.041
2.081AsnArg: 2.081 ± 0.043
2.221AsnSer: 2.221 ± 0.047
2.179AsnThr: 2.179 ± 0.051
2.601AsnVal: 2.601 ± 0.053
0.374AsnTrp: 0.374 ± 0.019
1.298AsnTyr: 1.298 ± 0.041
0.0AsnXaa: 0.0 ± 0.0
Pro
3.603ProAla: 3.603 ± 0.071
0.528ProCys: 0.528 ± 0.025
2.518ProAsp: 2.518 ± 0.061
3.614ProGlu: 3.614 ± 0.066
1.852ProPhe: 1.852 ± 0.05
2.975ProGly: 2.975 ± 0.06
0.731ProHis: 0.731 ± 0.029
2.285ProIle: 2.285 ± 0.05
2.001ProLys: 2.001 ± 0.05
3.484ProLeu: 3.484 ± 0.061
1.044ProMet: 1.044 ± 0.032
1.411ProAsn: 1.411 ± 0.036
1.312ProPro: 1.312 ± 0.039
1.786ProGln: 1.786 ± 0.043
1.534ProArg: 1.534 ± 0.046
2.253ProSer: 2.253 ± 0.047
1.892ProThr: 1.892 ± 0.06
3.534ProVal: 3.534 ± 0.082
0.369ProTrp: 0.369 ± 0.02
1.419ProTyr: 1.419 ± 0.04
0.0ProXaa: 0.0 ± 0.0
Gln
3.433GlnAla: 3.433 ± 0.067
0.391GlnCys: 0.391 ± 0.023
1.643GlnAsp: 1.643 ± 0.04
2.769GlnGlu: 2.769 ± 0.058
1.241GlnPhe: 1.241 ± 0.037
2.466GlnGly: 2.466 ± 0.052
0.597GlnHis: 0.597 ± 0.022
2.831GlnIle: 2.831 ± 0.059
2.867GlnLys: 2.867 ± 0.064
3.414GlnLeu: 3.414 ± 0.062
1.229GlnMet: 1.229 ± 0.042
1.863GlnAsn: 1.863 ± 0.044
1.358GlnPro: 1.358 ± 0.041
1.761GlnGln: 1.761 ± 0.05
1.91GlnArg: 1.91 ± 0.051
2.363GlnSer: 2.363 ± 0.056
2.138GlnThr: 2.138 ± 0.045
2.48GlnVal: 2.48 ± 0.049
0.323GlnTrp: 0.323 ± 0.019
1.373GlnTyr: 1.373 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
3.828ArgAla: 3.828 ± 0.065
0.713ArgCys: 0.713 ± 0.027
2.372ArgAsp: 2.372 ± 0.049
4.007ArgGlu: 4.007 ± 0.069
2.244ArgPhe: 2.244 ± 0.048
3.139ArgGly: 3.139 ± 0.062
0.948ArgHis: 0.948 ± 0.033
3.471ArgIle: 3.471 ± 0.063
2.924ArgLys: 2.924 ± 0.05
5.264ArgLeu: 5.264 ± 0.082
1.671ArgMet: 1.671 ± 0.045
1.865ArgAsn: 1.865 ± 0.04
1.852ArgPro: 1.852 ± 0.047
2.316ArgGln: 2.316 ± 0.059
3.2ArgArg: 3.2 ± 0.083
2.777ArgSer: 2.777 ± 0.062
2.322ArgThr: 2.322 ± 0.046
3.446ArgVal: 3.446 ± 0.07
0.489ArgTrp: 0.489 ± 0.024
1.794ArgTyr: 1.794 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
5.826SerAla: 5.826 ± 0.1
0.854SerCys: 0.854 ± 0.034
3.176SerAsp: 3.176 ± 0.054
3.692SerGlu: 3.692 ± 0.063
2.88SerPhe: 2.88 ± 0.06
5.951SerGly: 5.951 ± 0.086
1.11SerHis: 1.11 ± 0.037
4.213SerIle: 4.213 ± 0.067
2.887SerLys: 2.887 ± 0.06
6.047SerLeu: 6.047 ± 0.089
1.895SerMet: 1.895 ± 0.049
2.028SerAsn: 2.028 ± 0.042
2.452SerPro: 2.452 ± 0.049
2.132SerGln: 2.132 ± 0.053
3.183SerArg: 3.183 ± 0.056
4.675SerSer: 4.675 ± 0.125
3.158SerThr: 3.158 ± 0.057
4.877SerVal: 4.877 ± 0.078
0.513SerTrp: 0.513 ± 0.025
2.174SerTyr: 2.174 ± 0.047
0.0SerXaa: 0.0 ± 0.0
Thr
5.234ThrAla: 5.234 ± 0.078
0.612ThrCys: 0.612 ± 0.028
2.787ThrAsp: 2.787 ± 0.06
3.396ThrGlu: 3.396 ± 0.056
2.087ThrPhe: 2.087 ± 0.057
4.793ThrGly: 4.793 ± 0.08
0.95ThrHis: 0.95 ± 0.032
3.546ThrIle: 3.546 ± 0.059
2.425ThrLys: 2.425 ± 0.051
5.413ThrLeu: 5.413 ± 0.069
1.42ThrMet: 1.42 ± 0.04
1.802ThrAsn: 1.802 ± 0.041
2.606ThrPro: 2.606 ± 0.053
1.75ThrGln: 1.75 ± 0.041
2.164ThrArg: 2.164 ± 0.049
2.958ThrSer: 2.958 ± 0.056
2.694ThrThr: 2.694 ± 0.062
4.826ThrVal: 4.826 ± 0.078
0.411ThrTrp: 0.411 ± 0.023
1.682ThrTyr: 1.682 ± 0.048
0.0ThrXaa: 0.0 ± 0.0
Val
5.592ValAla: 5.592 ± 0.09
1.217ValCys: 1.217 ± 0.034
3.513ValAsp: 3.513 ± 0.066
4.181ValGlu: 4.181 ± 0.072
3.315ValPhe: 3.315 ± 0.059
4.719ValGly: 4.719 ± 0.086
1.16ValHis: 1.16 ± 0.038
4.956ValIle: 4.956 ± 0.089
3.503ValLys: 3.503 ± 0.062
7.656ValLeu: 7.656 ± 0.095
2.005ValMet: 2.005 ± 0.051
2.559ValAsn: 2.559 ± 0.05
3.126ValPro: 3.126 ± 0.054
2.446ValGln: 2.446 ± 0.056
3.565ValArg: 3.565 ± 0.066
5.063ValSer: 5.063 ± 0.069
4.263ValThr: 4.263 ± 0.074
5.147ValVal: 5.147 ± 0.081
0.638ValTrp: 0.638 ± 0.027
2.328ValTyr: 2.328 ± 0.051
0.0ValXaa: 0.0 ± 0.0
Trp
0.635TrpAla: 0.635 ± 0.03
0.134TrpCys: 0.134 ± 0.013
0.475TrpAsp: 0.475 ± 0.021
0.564TrpGlu: 0.564 ± 0.023
0.41TrpPhe: 0.41 ± 0.02
0.692TrpGly: 0.692 ± 0.03
0.178TrpHis: 0.178 ± 0.016
0.597TrpIle: 0.597 ± 0.023
0.538TrpLys: 0.538 ± 0.023
0.883TrpLeu: 0.883 ± 0.032
0.323TrpMet: 0.323 ± 0.016
0.464TrpAsn: 0.464 ± 0.02
0.284TrpPro: 0.284 ± 0.018
0.412TrpGln: 0.412 ± 0.019
0.426TrpArg: 0.426 ± 0.021
0.475TrpSer: 0.475 ± 0.023
0.356TrpThr: 0.356 ± 0.021
0.563TrpVal: 0.563 ± 0.022
0.123TrpTrp: 0.123 ± 0.014
0.282TrpTyr: 0.282 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.515TyrAla: 2.515 ± 0.05
0.575TyrCys: 0.575 ± 0.026
2.03TyrAsp: 2.03 ± 0.057
2.107TyrGlu: 2.107 ± 0.052
1.553TyrPhe: 1.553 ± 0.043
2.665TyrGly: 2.665 ± 0.047
0.74TyrHis: 0.74 ± 0.028
1.994TyrIle: 1.994 ± 0.046
1.356TyrLys: 1.356 ± 0.041
3.561TyrLeu: 3.561 ± 0.07
0.717TyrMet: 0.717 ± 0.029
1.279TyrAsn: 1.279 ± 0.037
1.492TyrPro: 1.492 ± 0.039
1.498TyrGln: 1.498 ± 0.041
2.147TyrArg: 2.147 ± 0.056
2.079TyrSer: 2.079 ± 0.048
1.897TyrThr: 1.897 ± 0.044
2.114TyrVal: 2.114 ± 0.046
0.302TyrTrp: 0.302 ± 0.016
1.404TyrTyr: 1.404 ± 0.042
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3478 proteins (987318 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski