Indibacter alkaliphilus LW1

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cyclobacteriaceae; Indibacter; Indibacter alkaliphilus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4650 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|S2DM90|S2DM90_9BACT Uncharacterized protein OS=Indibacter alkaliphilus LW1 OX=1189612 GN=A33Q_0106 PE=4 SV=1
MM1 pKa = 7.84RR2 pKa = 11.84KK3 pKa = 9.22LLKK6 pKa = 10.38YY7 pKa = 10.39LLVVLPFVVMAACVDD22 pKa = 3.66EE23 pKa = 4.9QNIEE27 pKa = 4.16DD28 pKa = 4.67LPPTSEE34 pKa = 4.31DD35 pKa = 2.91ATFSVEE41 pKa = 4.14PTSEE45 pKa = 3.81NPNIVNFTADD55 pKa = 3.26RR56 pKa = 11.84EE57 pKa = 4.32FFRR60 pKa = 11.84MQWDD64 pKa = 4.08LGNGTTVEE72 pKa = 4.1GRR74 pKa = 11.84TVTGTYY80 pKa = 9.82PNEE83 pKa = 3.72GTYY86 pKa = 10.03TVTLTVFNAAGSASFSQDD104 pKa = 2.32IVIDD108 pKa = 3.68QTDD111 pKa = 3.83PTLLDD116 pKa = 3.49SPIFNILTGGINAIEE131 pKa = 4.27GRR133 pKa = 11.84TWVIDD138 pKa = 3.6SARR141 pKa = 11.84VGHH144 pKa = 6.51FGVGPNPSQAGDD156 pKa = 3.56FPEE159 pKa = 4.8WYY161 pKa = 9.86AAQANEE167 pKa = 3.85KK168 pKa = 9.27RR169 pKa = 11.84GSGMYY174 pKa = 8.93TDD176 pKa = 5.11RR177 pKa = 11.84YY178 pKa = 9.18TFFLDD183 pKa = 3.25NFGFNMEE190 pKa = 4.53TNGFVYY196 pKa = 10.49LNEE199 pKa = 4.09AQGPNFPGAFDD210 pKa = 4.56PGVGDD215 pKa = 4.27LSAPYY220 pKa = 8.61EE221 pKa = 4.41APDD224 pKa = 3.72GLTWSYY230 pKa = 11.42TEE232 pKa = 4.09VEE234 pKa = 4.16NGFDD238 pKa = 3.56EE239 pKa = 5.13LRR241 pKa = 11.84ISEE244 pKa = 4.32GGFLGYY250 pKa = 9.96FAGGTTYY257 pKa = 10.63QIVNLEE263 pKa = 4.12EE264 pKa = 4.7NEE266 pKa = 4.03MFLRR270 pKa = 11.84YY271 pKa = 8.81IDD273 pKa = 3.64QANEE277 pKa = 3.33EE278 pKa = 4.32LAWYY282 pKa = 9.4IRR284 pKa = 11.84LIPAGFDD291 pKa = 3.12SGEE294 pKa = 4.08DD295 pKa = 3.59VQEE298 pKa = 4.36EE299 pKa = 4.52EE300 pKa = 4.43PSDD303 pKa = 3.63VNFNFEE309 pKa = 4.58DD310 pKa = 4.55LIGDD314 pKa = 4.47GTQAWTLKK322 pKa = 10.11SAAGAFGVGPAPGNDD337 pKa = 2.81SFFPNGNDD345 pKa = 3.0ISDD348 pKa = 3.64EE349 pKa = 4.13RR350 pKa = 11.84ACLFNDD356 pKa = 4.49LYY358 pKa = 10.77IFSEE362 pKa = 4.51DD363 pKa = 2.87GTYY366 pKa = 10.33EE367 pKa = 4.11YY368 pKa = 8.98DD369 pKa = 3.47TQGDD373 pKa = 3.77IFAEE377 pKa = 5.12FYY379 pKa = 10.35MGVSEE384 pKa = 5.44EE385 pKa = 4.48GCQDD389 pKa = 3.27EE390 pKa = 5.06SNLEE394 pKa = 4.12GTPGEE399 pKa = 3.96AWGSGVHH406 pKa = 5.96SFEE409 pKa = 4.12FTPGEE414 pKa = 4.23GSTRR418 pKa = 11.84PKK420 pKa = 9.51ITVTGTGAFVVLAKK434 pKa = 10.58AFNGGEE440 pKa = 4.13YY441 pKa = 10.3EE442 pKa = 4.2QGPPNEE448 pKa = 4.46NASVTYY454 pKa = 10.44DD455 pKa = 3.39VLDD458 pKa = 3.83YY459 pKa = 11.25DD460 pKa = 4.48PEE462 pKa = 4.65SKK464 pKa = 10.58EE465 pKa = 3.77LTLTIDD471 pKa = 3.13ITNDD475 pKa = 2.92GGVWWTFVLVPNEE488 pKa = 3.75

Molecular weight:
53.49 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|S2DKF0|S2DKF0_9BACT mRNA interferase OS=Indibacter alkaliphilus LW1 OX=1189612 GN=A33Q_0549 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.78HH16 pKa = 3.85GFRR19 pKa = 11.84LRR21 pKa = 11.84MSTANGRR28 pKa = 11.84RR29 pKa = 11.84VLKK32 pKa = 10.41SRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.19GRR39 pKa = 11.84HH40 pKa = 5.41KK41 pKa = 10.43LTVSSEE47 pKa = 4.01KK48 pKa = 9.88TLKK51 pKa = 10.51KK52 pKa = 10.72

Molecular weight:
6.26 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4650

0

4650

1482599

37

2932

318.8

36.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.411 ± 0.032

0.659 ± 0.011

5.43 ± 0.029

7.236 ± 0.036

5.524 ± 0.031

7.04 ± 0.04

1.856 ± 0.017

7.31 ± 0.03

6.872 ± 0.05

9.757 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.461 ± 0.017

5.296 ± 0.034

3.872 ± 0.023

3.619 ± 0.021

4.149 ± 0.027

6.548 ± 0.028

4.781 ± 0.023

6.154 ± 0.031

1.255 ± 0.016

3.768 ± 0.023

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski