Amino acid dipepetide frequency for Klebsiella phage KP27

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.051AlaAla: 4.051 ± 0.332
0.522AlaCys: 0.522 ± 0.098
3.799AlaAsp: 3.799 ± 0.269
4.699AlaGlu: 4.699 ± 0.349
2.809AlaPhe: 2.809 ± 0.25
4.159AlaGly: 4.159 ± 0.386
1.098AlaHis: 1.098 ± 0.146
5.041AlaIle: 5.041 ± 0.325
5.384AlaLys: 5.384 ± 0.379
5.654AlaLeu: 5.654 ± 0.329
2.107AlaMet: 2.107 ± 0.235
3.601AlaAsn: 3.601 ± 0.262
2.107AlaPro: 2.107 ± 0.223
2.233AlaGln: 2.233 ± 0.197
3.385AlaArg: 3.385 ± 0.264
3.637AlaSer: 3.637 ± 0.294
4.231AlaThr: 4.231 ± 0.426
4.609AlaVal: 4.609 ± 0.275
0.81AlaTrp: 0.81 ± 0.141
3.061AlaTyr: 3.061 ± 0.258
0.0AlaXaa: 0.0 ± 0.0
Cys
0.918CysAla: 0.918 ± 0.118
0.198CysCys: 0.198 ± 0.059
0.774CysAsp: 0.774 ± 0.124
0.774CysGlu: 0.774 ± 0.123
0.576CysPhe: 0.576 ± 0.099
1.062CysGly: 1.062 ± 0.167
0.306CysHis: 0.306 ± 0.071
0.558CysIle: 0.558 ± 0.097
0.972CysLys: 0.972 ± 0.129
0.9CysLeu: 0.9 ± 0.121
0.342CysMet: 0.342 ± 0.084
0.738CysAsn: 0.738 ± 0.131
0.54CysPro: 0.54 ± 0.108
0.27CysGln: 0.27 ± 0.078
0.612CysArg: 0.612 ± 0.108
0.684CysSer: 0.684 ± 0.116
0.72CysThr: 0.72 ± 0.101
0.792CysVal: 0.792 ± 0.12
0.144CysTrp: 0.144 ± 0.048
0.396CysTyr: 0.396 ± 0.076
0.0CysXaa: 0.0 ± 0.0
Asp
4.231AspAla: 4.231 ± 0.309
0.756AspCys: 0.756 ± 0.11
3.799AspAsp: 3.799 ± 0.298
4.915AspGlu: 4.915 ± 0.308
3.097AspPhe: 3.097 ± 0.234
4.627AspGly: 4.627 ± 0.331
0.936AspHis: 0.936 ± 0.127
4.843AspIle: 4.843 ± 0.33
4.357AspLys: 4.357 ± 0.31
5.059AspLeu: 5.059 ± 0.343
1.855AspMet: 1.855 ± 0.191
3.295AspAsn: 3.295 ± 0.248
2.953AspPro: 2.953 ± 0.221
1.728AspGln: 1.728 ± 0.164
2.953AspArg: 2.953 ± 0.222
3.889AspSer: 3.889 ± 0.266
3.853AspThr: 3.853 ± 0.254
4.105AspVal: 4.105 ± 0.299
1.224AspTrp: 1.224 ± 0.145
3.223AspTyr: 3.223 ± 0.292
0.0AspXaa: 0.0 ± 0.0
Glu
4.969GluAla: 4.969 ± 0.372
0.99GluCys: 0.99 ± 0.148
3.691GluAsp: 3.691 ± 0.274
5.239GluGlu: 5.239 ± 0.334
2.881GluPhe: 2.881 ± 0.213
3.781GluGly: 3.781 ± 0.283
1.53GluHis: 1.53 ± 0.19
5.528GluIle: 5.528 ± 0.343
5.311GluLys: 5.311 ± 0.391
5.96GluLeu: 5.96 ± 0.29
2.125GluMet: 2.125 ± 0.213
3.529GluAsn: 3.529 ± 0.24
1.368GluPro: 1.368 ± 0.173
2.413GluGln: 2.413 ± 0.231
3.169GluArg: 3.169 ± 0.294
4.231GluSer: 4.231 ± 0.31
4.069GluThr: 4.069 ± 0.317
4.375GluVal: 4.375 ± 0.284
1.116GluTrp: 1.116 ± 0.134
2.989GluTyr: 2.989 ± 0.269
0.0GluXaa: 0.0 ± 0.0
Phe
2.413PheAla: 2.413 ± 0.209
0.504PheCys: 0.504 ± 0.099
3.691PheAsp: 3.691 ± 0.234
2.953PheGlu: 2.953 ± 0.264
1.368PhePhe: 1.368 ± 0.165
3.169PheGly: 3.169 ± 0.237
0.558PheHis: 0.558 ± 0.11
2.503PheIle: 2.503 ± 0.234
2.953PheLys: 2.953 ± 0.243
2.539PheLeu: 2.539 ± 0.203
1.152PheMet: 1.152 ± 0.133
2.359PheAsn: 2.359 ± 0.183
1.422PhePro: 1.422 ± 0.161
1.224PheGln: 1.224 ± 0.147
1.71PheArg: 1.71 ± 0.171
2.521PheSer: 2.521 ± 0.205
2.701PheThr: 2.701 ± 0.223
3.133PheVal: 3.133 ± 0.219
0.702PheTrp: 0.702 ± 0.109
1.566PheTyr: 1.566 ± 0.18
0.0PheXaa: 0.0 ± 0.0
Gly
3.979GlyAla: 3.979 ± 0.38
0.99GlyCys: 0.99 ± 0.12
4.411GlyAsp: 4.411 ± 0.333
3.889GlyGlu: 3.889 ± 0.26
3.097GlyPhe: 3.097 ± 0.279
3.781GlyGly: 3.781 ± 0.403
1.08GlyHis: 1.08 ± 0.14
4.015GlyIle: 4.015 ± 0.233
4.897GlyLys: 4.897 ± 0.283
4.555GlyLeu: 4.555 ± 0.31
1.728GlyMet: 1.728 ± 0.176
3.655GlyAsn: 3.655 ± 0.347
0.774GlyPro: 0.774 ± 0.103
1.584GlyGln: 1.584 ± 0.191
3.007GlyArg: 3.007 ± 0.226
4.681GlySer: 4.681 ± 0.345
3.727GlyThr: 3.727 ± 0.381
5.113GlyVal: 5.113 ± 0.257
0.99GlyTrp: 0.99 ± 0.147
3.511GlyTyr: 3.511 ± 0.23
0.0GlyXaa: 0.0 ± 0.0
His
1.206HisAla: 1.206 ± 0.153
0.27HisCys: 0.27 ± 0.079
1.098HisAsp: 1.098 ± 0.162
1.224HisGlu: 1.224 ± 0.155
0.702HisPhe: 0.702 ± 0.114
0.99HisGly: 0.99 ± 0.132
0.396HisHis: 0.396 ± 0.095
1.26HisIle: 1.26 ± 0.152
1.17HisLys: 1.17 ± 0.165
1.458HisLeu: 1.458 ± 0.187
0.324HisMet: 0.324 ± 0.075
0.918HisAsn: 0.918 ± 0.132
0.936HisPro: 0.936 ± 0.122
0.612HisGln: 0.612 ± 0.1
0.882HisArg: 0.882 ± 0.126
1.206HisSer: 1.206 ± 0.153
0.72HisThr: 0.72 ± 0.113
1.116HisVal: 1.116 ± 0.127
0.27HisTrp: 0.27 ± 0.062
0.792HisTyr: 0.792 ± 0.114
0.0HisXaa: 0.0 ± 0.0
Ile
4.879IleAla: 4.879 ± 0.306
0.63IleCys: 0.63 ± 0.09
5.384IleAsp: 5.384 ± 0.309
4.609IleGlu: 4.609 ± 0.302
2.269IlePhe: 2.269 ± 0.204
3.889IleGly: 3.889 ± 0.286
1.17IleHis: 1.17 ± 0.157
3.817IleIle: 3.817 ± 0.277
5.095IleLys: 5.095 ± 0.315
4.015IleLeu: 4.015 ± 0.255
2.107IleMet: 2.107 ± 0.177
3.835IleAsn: 3.835 ± 0.259
2.809IlePro: 2.809 ± 0.232
2.305IleGln: 2.305 ± 0.181
3.907IleArg: 3.907 ± 0.277
3.943IleSer: 3.943 ± 0.284
4.465IleThr: 4.465 ± 0.261
5.239IleVal: 5.239 ± 0.302
0.684IleTrp: 0.684 ± 0.1
2.737IleTyr: 2.737 ± 0.196
0.0IleXaa: 0.0 ± 0.0
Lys
5.816LysAla: 5.816 ± 0.349
0.792LysCys: 0.792 ± 0.14
4.195LysAsp: 4.195 ± 0.301
5.636LysGlu: 5.636 ± 0.327
2.989LysPhe: 2.989 ± 0.226
4.303LysGly: 4.303 ± 0.276
1.512LysHis: 1.512 ± 0.198
4.825LysIle: 4.825 ± 0.287
4.915LysLys: 4.915 ± 0.354
5.762LysLeu: 5.762 ± 0.38
2.449LysMet: 2.449 ± 0.195
4.249LysAsn: 4.249 ± 0.287
2.647LysPro: 2.647 ± 0.205
2.863LysGln: 2.863 ± 0.267
3.601LysArg: 3.601 ± 0.296
3.637LysSer: 3.637 ± 0.255
4.393LysThr: 4.393 ± 0.224
4.663LysVal: 4.663 ± 0.285
0.918LysTrp: 0.918 ± 0.122
3.367LysTyr: 3.367 ± 0.266
0.0LysXaa: 0.0 ± 0.0
Leu
5.041LeuAla: 5.041 ± 0.318
1.044LeuCys: 1.044 ± 0.182
5.347LeuAsp: 5.347 ± 0.377
4.879LeuGlu: 4.879 ± 0.355
3.241LeuPhe: 3.241 ± 0.241
4.051LeuGly: 4.051 ± 0.242
1.458LeuHis: 1.458 ± 0.178
4.609LeuIle: 4.609 ± 0.287
5.474LeuLys: 5.474 ± 0.337
4.717LeuLeu: 4.717 ± 0.323
2.611LeuMet: 2.611 ± 0.208
4.123LeuAsn: 4.123 ± 0.243
3.331LeuPro: 3.331 ± 0.234
2.287LeuGln: 2.287 ± 0.228
4.087LeuArg: 4.087 ± 0.234
5.203LeuSer: 5.203 ± 0.299
4.357LeuThr: 4.357 ± 0.361
4.717LeuVal: 4.717 ± 0.287
0.738LeuTrp: 0.738 ± 0.116
3.637LeuTyr: 3.637 ± 0.261
0.0LeuXaa: 0.0 ± 0.0
Met
1.909MetAla: 1.909 ± 0.179
0.36MetCys: 0.36 ± 0.074
1.801MetAsp: 1.801 ± 0.193
1.801MetGlu: 1.801 ± 0.165
1.008MetPhe: 1.008 ± 0.148
1.494MetGly: 1.494 ± 0.162
0.504MetHis: 0.504 ± 0.095
2.341MetIle: 2.341 ± 0.233
2.899MetLys: 2.899 ± 0.245
2.107MetLeu: 2.107 ± 0.207
0.936MetMet: 0.936 ± 0.131
2.197MetAsn: 2.197 ± 0.191
0.792MetPro: 0.792 ± 0.126
1.188MetGln: 1.188 ± 0.163
1.152MetArg: 1.152 ± 0.143
1.909MetSer: 1.909 ± 0.179
1.692MetThr: 1.692 ± 0.184
1.674MetVal: 1.674 ± 0.174
0.414MetTrp: 0.414 ± 0.096
1.17MetTyr: 1.17 ± 0.135
0.0MetXaa: 0.0 ± 0.0
Asn
4.105AsnAla: 4.105 ± 0.282
0.612AsnCys: 0.612 ± 0.105
3.187AsnAsp: 3.187 ± 0.263
3.943AsnGlu: 3.943 ± 0.258
1.945AsnPhe: 1.945 ± 0.22
4.681AsnGly: 4.681 ± 0.388
1.116AsnHis: 1.116 ± 0.138
3.313AsnIle: 3.313 ± 0.21
3.403AsnLys: 3.403 ± 0.264
4.267AsnLeu: 4.267 ± 0.248
1.494AsnMet: 1.494 ± 0.146
3.151AsnAsn: 3.151 ± 0.256
2.737AsnPro: 2.737 ± 0.235
1.855AsnGln: 1.855 ± 0.222
2.683AsnArg: 2.683 ± 0.214
3.349AsnSer: 3.349 ± 0.276
3.223AsnThr: 3.223 ± 0.256
3.853AsnVal: 3.853 ± 0.271
0.378AsnTrp: 0.378 ± 0.078
1.945AsnTyr: 1.945 ± 0.216
0.0AsnXaa: 0.0 ± 0.0
Pro
2.125ProAla: 2.125 ± 0.202
0.432ProCys: 0.432 ± 0.076
3.313ProAsp: 3.313 ± 0.251
2.881ProGlu: 2.881 ± 0.235
1.548ProPhe: 1.548 ± 0.166
2.251ProGly: 2.251 ± 0.217
0.54ProHis: 0.54 ± 0.102
2.017ProIle: 2.017 ± 0.181
2.467ProLys: 2.467 ± 0.26
2.557ProLeu: 2.557 ± 0.251
0.63ProMet: 0.63 ± 0.099
1.602ProAsn: 1.602 ± 0.182
1.098ProPro: 1.098 ± 0.17
0.954ProGln: 0.954 ± 0.115
1.566ProArg: 1.566 ± 0.178
2.233ProSer: 2.233 ± 0.183
2.251ProThr: 2.251 ± 0.224
2.989ProVal: 2.989 ± 0.237
0.414ProTrp: 0.414 ± 0.082
1.602ProTyr: 1.602 ± 0.153
0.0ProXaa: 0.0 ± 0.0
Gln
2.449GlnAla: 2.449 ± 0.285
0.36GlnCys: 0.36 ± 0.095
1.548GlnAsp: 1.548 ± 0.178
2.053GlnGlu: 2.053 ± 0.208
1.26GlnPhe: 1.26 ± 0.13
1.62GlnGly: 1.62 ± 0.169
0.45GlnHis: 0.45 ± 0.088
2.755GlnIle: 2.755 ± 0.201
2.557GlnLys: 2.557 ± 0.286
2.899GlnLeu: 2.899 ± 0.244
0.972GlnMet: 0.972 ± 0.137
1.638GlnAsn: 1.638 ± 0.174
0.864GlnPro: 0.864 ± 0.11
1.386GlnGln: 1.386 ± 0.143
1.71GlnArg: 1.71 ± 0.18
1.71GlnSer: 1.71 ± 0.205
1.855GlnThr: 1.855 ± 0.164
2.233GlnVal: 2.233 ± 0.192
0.378GlnTrp: 0.378 ± 0.081
1.692GlnTyr: 1.692 ± 0.164
0.0GlnXaa: 0.0 ± 0.0
Arg
2.791ArgAla: 2.791 ± 0.186
0.594ArgCys: 0.594 ± 0.113
3.043ArgAsp: 3.043 ± 0.273
3.385ArgGlu: 3.385 ± 0.279
1.927ArgPhe: 1.927 ± 0.192
3.259ArgGly: 3.259 ± 0.242
0.702ArgHis: 0.702 ± 0.104
3.619ArgIle: 3.619 ± 0.305
3.511ArgLys: 3.511 ± 0.249
3.763ArgLeu: 3.763 ± 0.219
1.296ArgMet: 1.296 ± 0.149
3.043ArgAsn: 3.043 ± 0.219
1.476ArgPro: 1.476 ± 0.165
1.602ArgGln: 1.602 ± 0.178
2.089ArgArg: 2.089 ± 0.172
2.827ArgSer: 2.827 ± 0.235
2.449ArgThr: 2.449 ± 0.209
3.313ArgVal: 3.313 ± 0.259
0.864ArgTrp: 0.864 ± 0.121
2.053ArgTyr: 2.053 ± 0.184
0.0ArgXaa: 0.0 ± 0.0
Ser
3.781SerAla: 3.781 ± 0.291
0.702SerCys: 0.702 ± 0.117
3.943SerAsp: 3.943 ± 0.248
3.745SerGlu: 3.745 ± 0.259
2.701SerPhe: 2.701 ± 0.21
4.645SerGly: 4.645 ± 0.353
1.098SerHis: 1.098 ± 0.129
4.303SerIle: 4.303 ± 0.289
4.069SerLys: 4.069 ± 0.256
4.915SerLeu: 4.915 ± 0.333
1.945SerMet: 1.945 ± 0.191
2.953SerAsn: 2.953 ± 0.252
2.539SerPro: 2.539 ± 0.196
1.999SerGln: 1.999 ± 0.195
2.755SerArg: 2.755 ± 0.252
3.727SerSer: 3.727 ± 0.325
3.169SerThr: 3.169 ± 0.325
4.483SerVal: 4.483 ± 0.272
0.828SerTrp: 0.828 ± 0.117
2.467SerTyr: 2.467 ± 0.194
0.0SerXaa: 0.0 ± 0.0
Thr
3.871ThrAla: 3.871 ± 0.334
0.576ThrCys: 0.576 ± 0.105
3.403ThrAsp: 3.403 ± 0.258
3.727ThrGlu: 3.727 ± 0.269
2.575ThrPhe: 2.575 ± 0.245
4.177ThrGly: 4.177 ± 0.35
1.098ThrHis: 1.098 ± 0.128
4.051ThrIle: 4.051 ± 0.265
3.907ThrLys: 3.907 ± 0.271
5.203ThrLeu: 5.203 ± 0.335
1.404ThrMet: 1.404 ± 0.147
3.061ThrAsn: 3.061 ± 0.304
2.809ThrPro: 2.809 ± 0.234
1.692ThrGln: 1.692 ± 0.201
2.683ThrArg: 2.683 ± 0.204
3.367ThrSer: 3.367 ± 0.305
3.295ThrThr: 3.295 ± 0.297
4.753ThrVal: 4.753 ± 0.408
0.738ThrTrp: 0.738 ± 0.106
2.431ThrTyr: 2.431 ± 0.201
0.0ThrXaa: 0.0 ± 0.0
Val
4.699ValAla: 4.699 ± 0.348
1.098ValCys: 1.098 ± 0.156
5.005ValAsp: 5.005 ± 0.287
5.203ValGlu: 5.203 ± 0.339
2.935ValPhe: 2.935 ± 0.205
3.979ValGly: 3.979 ± 0.263
0.918ValHis: 0.918 ± 0.146
4.591ValIle: 4.591 ± 0.256
5.762ValLys: 5.762 ± 0.32
4.717ValLeu: 4.717 ± 0.292
1.963ValMet: 1.963 ± 0.209
3.727ValAsn: 3.727 ± 0.312
2.305ValPro: 2.305 ± 0.233
2.107ValGln: 2.107 ± 0.191
2.899ValArg: 2.899 ± 0.228
4.645ValSer: 4.645 ± 0.276
4.141ValThr: 4.141 ± 0.324
4.429ValVal: 4.429 ± 0.274
0.99ValTrp: 0.99 ± 0.148
3.511ValTyr: 3.511 ± 0.235
0.0ValXaa: 0.0 ± 0.0
Trp
0.918TrpAla: 0.918 ± 0.118
0.198TrpCys: 0.198 ± 0.054
0.972TrpAsp: 0.972 ± 0.136
0.756TrpGlu: 0.756 ± 0.134
0.666TrpPhe: 0.666 ± 0.103
0.666TrpGly: 0.666 ± 0.11
0.252TrpHis: 0.252 ± 0.069
0.792TrpIle: 0.792 ± 0.131
1.206TrpLys: 1.206 ± 0.141
1.008TrpLeu: 1.008 ± 0.117
0.63TrpMet: 0.63 ± 0.133
0.756TrpAsn: 0.756 ± 0.126
0.198TrpPro: 0.198 ± 0.061
0.54TrpGln: 0.54 ± 0.096
0.702TrpArg: 0.702 ± 0.112
0.612TrpSer: 0.612 ± 0.115
0.684TrpThr: 0.684 ± 0.103
0.882TrpVal: 0.882 ± 0.118
0.144TrpTrp: 0.144 ± 0.06
0.72TrpTyr: 0.72 ± 0.116
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.845TyrAla: 2.845 ± 0.241
0.648TyrCys: 0.648 ± 0.104
3.295TyrAsp: 3.295 ± 0.22
2.953TyrGlu: 2.953 ± 0.227
1.548TyrPhe: 1.548 ± 0.196
2.881TyrGly: 2.881 ± 0.25
0.828TyrHis: 0.828 ± 0.145
3.007TyrIle: 3.007 ± 0.246
3.223TyrLys: 3.223 ± 0.245
2.917TyrLeu: 2.917 ± 0.241
1.26TyrMet: 1.26 ± 0.158
2.809TyrAsn: 2.809 ± 0.249
1.764TyrPro: 1.764 ± 0.202
1.548TyrGln: 1.548 ± 0.177
2.053TyrArg: 2.053 ± 0.214
2.755TyrSer: 2.755 ± 0.231
2.827TyrThr: 2.827 ± 0.264
3.151TyrVal: 3.151 ± 0.234
0.558TyrTrp: 0.558 ± 0.101
1.873TyrTyr: 1.873 ± 0.199
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 276 proteins (55541 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski