Pleurotus ostreatus PC15

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Pleurotaceae; Pleurotus; Pleurotus ostreatus

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12186 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A067NUX7|A0A067NUX7_PLEOS WW domain-containing protein OS=Pleurotus ostreatus PC15 OX=1137138 GN=PLEOSDRAFT_1053128 PE=3 SV=1
MM1 pKa = 7.69APFTHH6 pKa = 7.46LIFLAIAFGSFTQVLAKK23 pKa = 10.1PVSTDD28 pKa = 3.19DD29 pKa = 4.03ALVEE33 pKa = 4.22RR34 pKa = 11.84DD35 pKa = 3.85DD36 pKa = 5.91GSLEE40 pKa = 3.89LCRR43 pKa = 11.84TINCATGNSEE53 pKa = 4.35PPPEE57 pKa = 4.48SFSICCSGGPTAASDD72 pKa = 3.98PNSDD76 pKa = 3.71SGSTTGDD83 pKa = 2.99TRR85 pKa = 11.84SGPNSPSGSTSSGDD99 pKa = 3.62TPNSSTGIIAATGYY113 pKa = 10.51SPNTKK118 pKa = 9.98EE119 pKa = 4.09SGSVGDD125 pKa = 4.83SPNGSQPTEE134 pKa = 3.72VTTNYY139 pKa = 10.73DD140 pKa = 3.24DD141 pKa = 5.86GKK143 pKa = 8.32PQPVGVGLKK152 pKa = 10.43FGGDD156 pKa = 3.32QLVALGPQFNGVAGAGVVINVPQTPTLPGPTQDD189 pKa = 4.24APSGPSSGDD198 pKa = 3.35PPTSGSNGGNTGNGSDD214 pKa = 3.95GGSGNTGGSSTSGPSNGNQGNGSGGANGDD243 pKa = 3.67TGNTGGPSTSGANGGNQGTGSGGPAAGAGNAGNSSNFPPSGADD286 pKa = 3.48LANAAADD293 pKa = 3.84NQGSGDD299 pKa = 3.33QGTNGNGSGSGTGSGAGSGQQGSNANGSGGSAASGQGSNPTSSPANGGSGSPLTCNCVCGG359 pKa = 3.73

Molecular weight: 33.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A067PBB4|A0A067PBB4_PLEOS PHD domain-containing protein (Fragment) OS=Pleurotus ostreatus PC15 OX=1137138 GN=PLEOSDRAFT_1029425 PE=4 SV=1
MM1 pKa = 7.51PRR3 pKa = 11.84LPPSLTRR10 pKa = 11.84LLSRR14 pKa = 11.84PPSLQTVSSTLRR26 pKa = 11.84PFAAASAMQVSNTITKK42 pKa = 10.41LPFITHH48 pKa = 7.28PFLKK52 pKa = 10.33SANPSFLASAWRR64 pKa = 11.84TTSLQPPTILWSVVQARR81 pKa = 11.84FGARR85 pKa = 11.84GAEE88 pKa = 4.05YY89 pKa = 10.4QPSQRR94 pKa = 11.84KK95 pKa = 8.85RR96 pKa = 11.84KK97 pKa = 9.17RR98 pKa = 11.84KK99 pKa = 9.56HH100 pKa = 5.55GFLARR105 pKa = 11.84KK106 pKa = 9.53RR107 pKa = 11.84SVSGRR112 pKa = 11.84KK113 pKa = 9.02VLARR117 pKa = 11.84RR118 pKa = 11.84LAKK121 pKa = 9.36GRR123 pKa = 11.84KK124 pKa = 7.83YY125 pKa = 10.65LSHH128 pKa = 7.04

Molecular weight: 14.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12186

0

12186

5090183

49

4851

417.7

46.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.547 ± 0.019

1.251 ± 0.009

5.689 ± 0.015

5.738 ± 0.021

3.774 ± 0.013

6.582 ± 0.022

2.552 ± 0.011

5.026 ± 0.015

4.564 ± 0.019

9.173 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.065 ± 0.007

3.545 ± 0.013

6.516 ± 0.028

3.676 ± 0.011

6.116 ± 0.019

8.545 ± 0.028

6.085 ± 0.015

6.384 ± 0.017

1.447 ± 0.008

2.727 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski