Amino acid dipepetide frequency for Leifsonia sp. Root60

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.75AlaAla: 20.75 ± 0.249
0.605AlaCys: 0.605 ± 0.025
8.405AlaAsp: 8.405 ± 0.106
7.966AlaGlu: 7.966 ± 0.093
4.027AlaPhe: 4.027 ± 0.063
12.113AlaGly: 12.113 ± 0.12
2.239AlaHis: 2.239 ± 0.052
6.484AlaIle: 6.484 ± 0.081
2.76AlaLys: 2.76 ± 0.066
13.649AlaLeu: 13.649 ± 0.136
2.626AlaMet: 2.626 ± 0.059
2.339AlaAsn: 2.339 ± 0.052
6.222AlaPro: 6.222 ± 0.091
3.486AlaGln: 3.486 ± 0.058
8.554AlaArg: 8.554 ± 0.116
8.392AlaSer: 8.392 ± 0.108
7.669AlaThr: 7.669 ± 0.091
12.003AlaVal: 12.003 ± 0.146
1.88AlaTrp: 1.88 ± 0.05
2.329AlaTyr: 2.329 ± 0.048
0.001AlaXaa: 0.001 ± 0.001
Cys
0.575CysAla: 0.575 ± 0.024
0.041CysCys: 0.041 ± 0.006
0.302CysAsp: 0.302 ± 0.017
0.205CysGlu: 0.205 ± 0.015
0.159CysPhe: 0.159 ± 0.013
0.483CysGly: 0.483 ± 0.021
0.1CysHis: 0.1 ± 0.011
0.202CysIle: 0.202 ± 0.012
0.062CysLys: 0.062 ± 0.008
0.4CysLeu: 0.4 ± 0.021
0.072CysMet: 0.072 ± 0.009
0.095CysAsn: 0.095 ± 0.01
0.262CysPro: 0.262 ± 0.015
0.1CysGln: 0.1 ± 0.01
0.26CysArg: 0.26 ± 0.015
0.361CysSer: 0.361 ± 0.02
0.327CysThr: 0.327 ± 0.021
0.395CysVal: 0.395 ± 0.019
0.074CysTrp: 0.074 ± 0.009
0.1CysTyr: 0.1 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
9.813AspAla: 9.813 ± 0.121
0.239AspCys: 0.239 ± 0.016
4.502AspAsp: 4.502 ± 0.082
4.275AspGlu: 4.275 ± 0.059
1.885AspPhe: 1.885 ± 0.049
6.416AspGly: 6.416 ± 0.092
1.09AspHis: 1.09 ± 0.033
2.584AspIle: 2.584 ± 0.057
1.025AspLys: 1.025 ± 0.038
5.967AspLeu: 5.967 ± 0.076
0.766AspMet: 0.766 ± 0.025
1.039AspAsn: 1.039 ± 0.034
3.92AspPro: 3.92 ± 0.069
1.546AspGln: 1.546 ± 0.035
4.292AspArg: 4.292 ± 0.064
3.053AspSer: 3.053 ± 0.059
2.957AspThr: 2.957 ± 0.05
5.526AspVal: 5.526 ± 0.081
1.02AspTrp: 1.02 ± 0.035
1.426AspTyr: 1.426 ± 0.039
0.0AspXaa: 0.0 ± 0.0
Glu
6.824GluAla: 6.824 ± 0.087
0.187GluCys: 0.187 ± 0.014
2.409GluAsp: 2.409 ± 0.049
2.766GluGlu: 2.766 ± 0.055
1.791GluPhe: 1.791 ± 0.042
3.546GluGly: 3.546 ± 0.071
1.433GluHis: 1.433 ± 0.036
2.776GluIle: 2.776 ± 0.055
1.556GluLys: 1.556 ± 0.043
6.754GluLeu: 6.754 ± 0.09
0.961GluMet: 0.961 ± 0.031
1.317GluAsn: 1.317 ± 0.037
2.946GluPro: 2.946 ± 0.063
2.173GluGln: 2.173 ± 0.051
4.829GluArg: 4.829 ± 0.08
3.117GluSer: 3.117 ± 0.065
3.003GluThr: 3.003 ± 0.059
4.26GluVal: 4.26 ± 0.062
0.849GluTrp: 0.849 ± 0.029
1.167GluTyr: 1.167 ± 0.039
0.001GluXaa: 0.001 ± 0.001
Phe
4.289PheAla: 4.289 ± 0.07
0.158PheCys: 0.158 ± 0.011
2.462PheAsp: 2.462 ± 0.055
1.783PheGlu: 1.783 ± 0.05
1.129PhePhe: 1.129 ± 0.037
3.59PheGly: 3.59 ± 0.073
0.564PheHis: 0.564 ± 0.023
1.41PheIle: 1.41 ± 0.042
0.477PheLys: 0.477 ± 0.02
2.966PheLeu: 2.966 ± 0.067
0.425PheMet: 0.425 ± 0.021
0.682PheAsn: 0.682 ± 0.026
1.453PhePro: 1.453 ± 0.04
0.783PheGln: 0.783 ± 0.03
1.791PheArg: 1.791 ± 0.044
2.046PheSer: 2.046 ± 0.04
2.3PheThr: 2.3 ± 0.052
2.811PheVal: 2.811 ± 0.056
0.587PheTrp: 0.587 ± 0.027
0.709PheTyr: 0.709 ± 0.031
0.0PheXaa: 0.0 ± 0.0
Gly
10.48GlyAla: 10.48 ± 0.124
0.563GlyCys: 0.563 ± 0.027
5.107GlyAsp: 5.107 ± 0.067
4.745GlyGlu: 4.745 ± 0.073
3.398GlyPhe: 3.398 ± 0.062
7.705GlyGly: 7.705 ± 0.115
1.719GlyHis: 1.719 ± 0.045
5.122GlyIle: 5.122 ± 0.07
2.041GlyLys: 2.041 ± 0.051
8.833GlyLeu: 8.833 ± 0.1
1.876GlyMet: 1.876 ± 0.041
1.782GlyAsn: 1.782 ± 0.051
3.675GlyPro: 3.675 ± 0.067
2.308GlyGln: 2.308 ± 0.048
6.048GlyArg: 6.048 ± 0.079
6.113GlySer: 6.113 ± 0.084
5.768GlyThr: 5.768 ± 0.092
7.941GlyVal: 7.941 ± 0.091
1.64GlyTrp: 1.64 ± 0.041
2.248GlyTyr: 2.248 ± 0.048
0.0GlyXaa: 0.0 ± 0.0
His
2.076HisAla: 2.076 ± 0.049
0.103HisCys: 0.103 ± 0.01
1.294HisAsp: 1.294 ± 0.035
1.027HisGlu: 1.027 ± 0.034
0.589HisPhe: 0.589 ± 0.027
1.852HisGly: 1.852 ± 0.054
0.514HisHis: 0.514 ± 0.027
0.762HisIle: 0.762 ± 0.024
0.248HisLys: 0.248 ± 0.016
1.979HisLeu: 1.979 ± 0.045
0.303HisMet: 0.303 ± 0.018
0.34HisAsn: 0.34 ± 0.017
1.491HisPro: 1.491 ± 0.038
0.482HisGln: 0.482 ± 0.019
1.437HisArg: 1.437 ± 0.04
1.091HisSer: 1.091 ± 0.032
0.899HisThr: 0.899 ± 0.028
1.541HisVal: 1.541 ± 0.037
0.278HisTrp: 0.278 ± 0.016
0.454HisTyr: 0.454 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
7.475IleAla: 7.475 ± 0.077
0.234IleCys: 0.234 ± 0.015
3.841IleAsp: 3.841 ± 0.059
3.02IleGlu: 3.02 ± 0.062
1.308IlePhe: 1.308 ± 0.049
5.054IleGly: 5.054 ± 0.088
0.675IleHis: 0.675 ± 0.023
2.254IleIle: 2.254 ± 0.052
0.826IleLys: 0.826 ± 0.033
3.909IleLeu: 3.909 ± 0.074
0.669IleMet: 0.669 ± 0.024
0.934IleAsn: 0.934 ± 0.033
2.546IlePro: 2.546 ± 0.052
0.998IleGln: 0.998 ± 0.039
2.897IleArg: 2.897 ± 0.062
2.621IleSer: 2.621 ± 0.045
3.162IleThr: 3.162 ± 0.063
5.121IleVal: 5.121 ± 0.073
0.593IleTrp: 0.593 ± 0.028
0.819IleTyr: 0.819 ± 0.031
0.0IleXaa: 0.0 ± 0.0
Lys
2.584LysAla: 2.584 ± 0.071
0.059LysCys: 0.059 ± 0.008
1.114LysAsp: 1.114 ± 0.036
0.917LysGlu: 0.917 ± 0.032
0.505LysPhe: 0.505 ± 0.022
1.527LysGly: 1.527 ± 0.05
0.409LysHis: 0.409 ± 0.018
0.928LysIle: 0.928 ± 0.033
0.807LysLys: 0.807 ± 0.03
2.095LysLeu: 2.095 ± 0.053
0.368LysMet: 0.368 ± 0.019
0.543LysAsn: 0.543 ± 0.024
1.284LysPro: 1.284 ± 0.041
0.731LysGln: 0.731 ± 0.029
1.578LysArg: 1.578 ± 0.043
1.191LysSer: 1.191 ± 0.035
1.349LysThr: 1.349 ± 0.045
1.734LysVal: 1.734 ± 0.047
0.244LysTrp: 0.244 ± 0.016
0.515LysTyr: 0.515 ± 0.024
0.0LysXaa: 0.0 ± 0.0
Leu
14.617LeuAla: 14.617 ± 0.137
0.491LeuCys: 0.491 ± 0.02
6.726LeuAsp: 6.726 ± 0.089
5.228LeuGlu: 5.228 ± 0.079
3.058LeuPhe: 3.058 ± 0.07
9.14LeuGly: 9.14 ± 0.108
1.787LeuHis: 1.787 ± 0.041
4.68LeuIle: 4.68 ± 0.083
1.867LeuLys: 1.867 ± 0.046
10.143LeuLeu: 10.143 ± 0.129
1.493LeuMet: 1.493 ± 0.038
1.828LeuAsn: 1.828 ± 0.045
5.476LeuPro: 5.476 ± 0.071
2.505LeuGln: 2.505 ± 0.05
6.888LeuArg: 6.888 ± 0.097
6.129LeuSer: 6.129 ± 0.081
6.089LeuThr: 6.089 ± 0.083
9.398LeuVal: 9.398 ± 0.102
1.299LeuTrp: 1.299 ± 0.04
1.628LeuTyr: 1.628 ± 0.041
0.004LeuXaa: 0.004 ± 0.002
Met
2.129MetAla: 2.129 ± 0.047
0.075MetCys: 0.075 ± 0.009
0.742MetAsp: 0.742 ± 0.028
0.603MetGlu: 0.603 ± 0.027
0.514MetPhe: 0.514 ± 0.022
1.218MetGly: 1.218 ± 0.034
0.323MetHis: 0.323 ± 0.016
0.868MetIle: 0.868 ± 0.032
0.465MetLys: 0.465 ± 0.024
1.929MetLeu: 1.929 ± 0.048
0.29MetMet: 0.29 ± 0.02
0.47MetAsn: 0.47 ± 0.02
1.103MetPro: 1.103 ± 0.03
0.552MetGln: 0.552 ± 0.023
1.251MetArg: 1.251 ± 0.032
1.389MetSer: 1.389 ± 0.032
1.657MetThr: 1.657 ± 0.038
1.298MetVal: 1.298 ± 0.033
0.182MetTrp: 0.182 ± 0.014
0.259MetTyr: 0.259 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
2.607AsnAla: 2.607 ± 0.052
0.123AsnCys: 0.123 ± 0.01
1.223AsnAsp: 1.223 ± 0.037
0.981AsnGlu: 0.981 ± 0.034
0.664AsnPhe: 0.664 ± 0.023
2.02AsnGly: 2.02 ± 0.051
0.395AsnHis: 0.395 ± 0.017
0.925AsnIle: 0.925 ± 0.031
0.399AsnLys: 0.399 ± 0.022
1.875AsnLeu: 1.875 ± 0.046
0.324AsnMet: 0.324 ± 0.016
0.469AsnAsn: 0.469 ± 0.022
1.625AsnPro: 1.625 ± 0.037
0.55AsnGln: 0.55 ± 0.026
1.275AsnArg: 1.275 ± 0.038
1.074AsnSer: 1.074 ± 0.033
1.22AsnThr: 1.22 ± 0.037
1.689AsnVal: 1.689 ± 0.043
0.313AsnTrp: 0.313 ± 0.018
0.497AsnTyr: 0.497 ± 0.023
0.0AsnXaa: 0.0 ± 0.0
Pro
6.968ProAla: 6.968 ± 0.081
0.146ProCys: 0.146 ± 0.011
4.065ProAsp: 4.065 ± 0.069
3.609ProGlu: 3.609 ± 0.065
1.769ProPhe: 1.769 ± 0.042
4.769ProGly: 4.769 ± 0.077
0.945ProHis: 0.945 ± 0.032
2.272ProIle: 2.272 ± 0.049
1.042ProLys: 1.042 ± 0.038
4.775ProLeu: 4.775 ± 0.07
0.813ProMet: 0.813 ± 0.025
1.082ProAsn: 1.082 ± 0.03
2.205ProPro: 2.205 ± 0.063
1.438ProGln: 1.438 ± 0.038
3.055ProArg: 3.055 ± 0.057
3.417ProSer: 3.417 ± 0.057
3.517ProThr: 3.517 ± 0.066
4.907ProVal: 4.907 ± 0.075
0.857ProTrp: 0.857 ± 0.033
1.025ProTyr: 1.025 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
3.487GlnAla: 3.487 ± 0.062
0.101GlnCys: 0.101 ± 0.009
1.229GlnAsp: 1.229 ± 0.039
1.178GlnGlu: 1.178 ± 0.035
0.864GlnPhe: 0.864 ± 0.029
1.925GlnGly: 1.925 ± 0.044
0.628GlnHis: 0.628 ± 0.025
1.359GlnIle: 1.359 ± 0.036
0.73GlnLys: 0.73 ± 0.028
3.064GlnLeu: 3.064 ± 0.055
0.441GlnMet: 0.441 ± 0.022
0.631GlnAsn: 0.631 ± 0.03
1.534GlnPro: 1.534 ± 0.036
1.055GlnGln: 1.055 ± 0.034
2.185GlnArg: 2.185 ± 0.052
1.567GlnSer: 1.567 ± 0.043
1.475GlnThr: 1.475 ± 0.038
2.262GlnVal: 2.262 ± 0.044
0.422GlnTrp: 0.422 ± 0.02
0.653GlnTyr: 0.653 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
8.224ArgAla: 8.224 ± 0.096
0.256ArgCys: 0.256 ± 0.017
4.047ArgAsp: 4.047 ± 0.068
3.853ArgGlu: 3.853 ± 0.066
2.383ArgPhe: 2.383 ± 0.053
5.061ArgGly: 5.061 ± 0.071
1.46ArgHis: 1.46 ± 0.042
3.699ArgIle: 3.699 ± 0.059
1.294ArgLys: 1.294 ± 0.034
6.979ArgLeu: 6.979 ± 0.091
1.77ArgMet: 1.77 ± 0.043
1.36ArgAsn: 1.36 ± 0.038
3.323ArgPro: 3.323 ± 0.065
1.851ArgGln: 1.851 ± 0.045
6.022ArgArg: 6.022 ± 0.097
4.325ArgSer: 4.325 ± 0.067
4.017ArgThr: 4.017 ± 0.065
5.568ArgVal: 5.568 ± 0.072
1.088ArgTrp: 1.088 ± 0.033
1.508ArgTyr: 1.508 ± 0.037
0.0ArgXaa: 0.0 ± 0.0
Ser
7.835SerAla: 7.835 ± 0.102
0.262SerCys: 0.262 ± 0.018
3.585SerAsp: 3.585 ± 0.058
3.036SerGlu: 3.036 ± 0.052
2.059SerPhe: 2.059 ± 0.046
6.409SerGly: 6.409 ± 0.084
1.064SerHis: 1.064 ± 0.033
3.052SerIle: 3.052 ± 0.053
1.235SerLys: 1.235 ± 0.041
5.661SerLeu: 5.661 ± 0.08
1.168SerMet: 1.168 ± 0.036
1.195SerAsn: 1.195 ± 0.039
3.213SerPro: 3.213 ± 0.05
1.448SerGln: 1.448 ± 0.044
3.828SerArg: 3.828 ± 0.071
3.96SerSer: 3.96 ± 0.076
4.121SerThr: 4.121 ± 0.069
5.525SerVal: 5.525 ± 0.08
0.975SerTrp: 0.975 ± 0.035
1.386SerTyr: 1.386 ± 0.039
0.0SerXaa: 0.0 ± 0.0
Thr
8.006ThrAla: 8.006 ± 0.095
0.27ThrCys: 0.27 ± 0.02
4.285ThrAsp: 4.285 ± 0.071
3.117ThrGlu: 3.117 ± 0.052
1.933ThrPhe: 1.933 ± 0.042
5.91ThrGly: 5.91 ± 0.076
1.065ThrHis: 1.065 ± 0.035
3.06ThrIle: 3.06 ± 0.057
1.243ThrLys: 1.243 ± 0.042
5.877ThrLeu: 5.877 ± 0.082
0.948ThrMet: 0.948 ± 0.032
1.28ThrAsn: 1.28 ± 0.037
3.901ThrPro: 3.901 ± 0.066
1.526ThrGln: 1.526 ± 0.042
3.622ThrArg: 3.622 ± 0.06
3.628ThrSer: 3.628 ± 0.072
4.084ThrThr: 4.084 ± 0.075
5.807ThrVal: 5.807 ± 0.09
0.875ThrTrp: 0.875 ± 0.03
1.123ThrTyr: 1.123 ± 0.04
0.0ThrXaa: 0.0 ± 0.0
Val
11.723ValAla: 11.723 ± 0.11
0.44ValCys: 0.44 ± 0.02
5.859ValAsp: 5.859 ± 0.075
4.6ValGlu: 4.6 ± 0.069
3.012ValPhe: 3.012 ± 0.056
7.339ValGly: 7.339 ± 0.101
1.708ValHis: 1.708 ± 0.046
4.945ValIle: 4.945 ± 0.082
1.686ValLys: 1.686 ± 0.041
9.606ValLeu: 9.606 ± 0.094
1.372ValMet: 1.372 ± 0.036
1.899ValAsn: 1.899 ± 0.047
4.737ValPro: 4.737 ± 0.076
2.203ValGln: 2.203 ± 0.047
5.658ValArg: 5.658 ± 0.073
5.238ValSer: 5.238 ± 0.078
5.798ValThr: 5.798 ± 0.089
9.165ValVal: 9.165 ± 0.102
1.139ValTrp: 1.139 ± 0.033
1.616ValTyr: 1.616 ± 0.04
0.001ValXaa: 0.001 ± 0.001
Trp
1.603TrpAla: 1.603 ± 0.036
0.093TrpCys: 0.093 ± 0.01
0.776TrpAsp: 0.776 ± 0.029
0.622TrpGlu: 0.622 ± 0.026
0.604TrpPhe: 0.604 ± 0.025
1.103TrpGly: 1.103 ± 0.04
0.348TrpHis: 0.348 ± 0.016
0.819TrpIle: 0.819 ± 0.03
0.344TrpLys: 0.344 ± 0.018
1.767TrpLeu: 1.767 ± 0.049
0.369TrpMet: 0.369 ± 0.022
0.501TrpAsn: 0.501 ± 0.021
0.722TrpPro: 0.722 ± 0.029
0.525TrpGln: 0.525 ± 0.022
1.126TrpArg: 1.126 ± 0.037
0.976TrpSer: 0.976 ± 0.031
0.908TrpThr: 0.908 ± 0.026
1.145TrpVal: 1.145 ± 0.038
0.362TrpTrp: 0.362 ± 0.019
0.308TrpTyr: 0.308 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.432TyrAla: 2.432 ± 0.05
0.13TyrCys: 0.13 ± 0.011
1.368TyrAsp: 1.368 ± 0.046
1.136TyrGlu: 1.136 ± 0.033
0.763TyrPhe: 0.763 ± 0.028
1.929TyrGly: 1.929 ± 0.047
0.294TyrHis: 0.294 ± 0.018
0.756TyrIle: 0.756 ± 0.027
0.376TyrLys: 0.376 ± 0.021
2.244TyrLeu: 2.244 ± 0.051
0.26TyrMet: 0.26 ± 0.017
0.463TyrAsn: 0.463 ± 0.023
1.034TyrPro: 1.034 ± 0.033
0.531TyrGln: 0.531 ± 0.027
1.492TyrArg: 1.492 ± 0.037
1.329TyrSer: 1.329 ± 0.036
1.242TyrThr: 1.242 ± 0.041
1.649TyrVal: 1.649 ± 0.039
0.345TyrTrp: 0.345 ± 0.022
0.482TyrTyr: 0.482 ± 0.022
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.003XaaAla: 0.003 ± 0.002
0.0XaaCys: 0.0 ± 0.0
0.001XaaAsp: 0.001 ± 0.001
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.001XaaGly: 0.001 ± 0.001
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.001XaaPro: 0.001 ± 0.001
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.001XaaSer: 0.001 ± 0.001
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3234 proteins (1047850 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski