Amino acid dipepetide frequency for Streptomyces sp. FXJ1.172

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.694AlaAla: 21.694 ± 0.139	1.118AlaCys: 1.118 ± 0.019	8.103AlaAsp: 8.103 ± 0.076	8.731AlaGlu: 8.731 ± 0.099	3.559AlaPhe: 3.559 ± 0.039	13.524AlaGly: 13.524 ± 0.084	3.092AlaHis: 3.092 ± 0.033	3.398AlaIle: 3.398 ± 0.041	2.875AlaLys: 2.875 ± 0.041	14.755AlaLeu: 14.755 ± 0.096	2.465AlaMet: 2.465 ± 0.033	1.93AlaAsn: 1.93 ± 0.033	7.146AlaPro: 7.146 ± 0.08	4.113AlaGln: 4.113 ± 0.043	10.518AlaArg: 10.518 ± 0.082	5.953AlaSer: 5.953 ± 0.057	6.966AlaThr: 6.966 ± 0.057	12.474AlaVal: 12.474 ± 0.079	1.925AlaTrp: 1.925 ± 0.03	2.826AlaTyr: 2.826 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	1.133CysAla: 1.133 ± 0.022	0.105CysCys: 0.105 ± 0.007	0.477CysAsp: 0.477 ± 0.016	0.411CysGlu: 0.411 ± 0.014	0.225CysPhe: 0.225 ± 0.01	0.992CysGly: 0.992 ± 0.023	0.203CysHis: 0.203 ± 0.009	0.181CysIle: 0.181 ± 0.01	0.116CysLys: 0.116 ± 0.007	0.8CysLeu: 0.8 ± 0.019	0.12CysMet: 0.12 ± 0.007	0.142CysAsn: 0.142 ± 0.009	0.499CysPro: 0.499 ± 0.017	0.184CysGln: 0.184 ± 0.008	0.604CysArg: 0.604 ± 0.019	0.433CysSer: 0.433 ± 0.015	0.561CysThr: 0.561 ± 0.015	0.693CysVal: 0.693 ± 0.017	0.135CysTrp: 0.135 ± 0.008	0.163CysTyr: 0.163 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.409AspAla: 7.409 ± 0.067	0.429AspCys: 0.429 ± 0.012	3.343AspAsp: 3.343 ± 0.043	3.552AspGlu: 3.552 ± 0.042	1.654AspPhe: 1.654 ± 0.024	6.078AspGly: 6.078 ± 0.064	1.472AspHis: 1.472 ± 0.022	1.849AspIle: 1.849 ± 0.03	1.198AspLys: 1.198 ± 0.025	6.108AspLeu: 6.108 ± 0.06	0.821AspMet: 0.821 ± 0.015	0.974AspAsn: 0.974 ± 0.022	4.424AspPro: 4.424 ± 0.045	1.604AspGln: 1.604 ± 0.026	4.643AspArg: 4.643 ± 0.051	2.505AspSer: 2.505 ± 0.035	3.241AspThr: 3.241 ± 0.038	4.531AspVal: 4.531 ± 0.044	1.027AspTrp: 1.027 ± 0.02	1.098AspTyr: 1.098 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	7.171GluAla: 7.171 ± 0.077	0.361GluCys: 0.361 ± 0.013	2.722GluAsp: 2.722 ± 0.039	3.538GluGlu: 3.538 ± 0.051	1.456GluPhe: 1.456 ± 0.029	4.2GluGly: 4.2 ± 0.052	1.524GluHis: 1.524 ± 0.026	2.154GluIle: 2.154 ± 0.032	1.351GluLys: 1.351 ± 0.027	6.575GluLeu: 6.575 ± 0.063	0.867GluMet: 0.867 ± 0.019	0.952GluAsn: 0.952 ± 0.02	3.289GluPro: 3.289 ± 0.044	2.252GluGln: 2.252 ± 0.034	5.33GluArg: 5.33 ± 0.061	2.412GluSer: 2.412 ± 0.031	2.703GluThr: 2.703 ± 0.04	4.276GluVal: 4.276 ± 0.051	0.71GluTrp: 0.71 ± 0.018	1.054GluTyr: 1.054 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	3.8PheAla: 3.8 ± 0.043	0.28PheCys: 0.28 ± 0.011	1.873PheAsp: 1.873 ± 0.027	1.398PheGlu: 1.398 ± 0.024	0.899PhePhe: 0.899 ± 0.02	3.075PheGly: 3.075 ± 0.039	0.643PheHis: 0.643 ± 0.016	0.68PheIle: 0.68 ± 0.016	0.526PheLys: 0.526 ± 0.014	2.617PheLeu: 2.617 ± 0.038	0.389PheMet: 0.389 ± 0.014	0.571PheAsn: 0.571 ± 0.016	1.407PhePro: 1.407 ± 0.024	0.719PheGln: 0.719 ± 0.018	1.857PheArg: 1.857 ± 0.028	1.469PheSer: 1.469 ± 0.024	2.08PheThr: 2.08 ± 0.031	2.187PheVal: 2.187 ± 0.031	0.414PheTrp: 0.414 ± 0.014	0.59PheTyr: 0.59 ± 0.014	0.0PheXaa: 0.0 ± 0.0
Gly	11.178GlyAla: 11.178 ± 0.082	0.861GlyCys: 0.861 ± 0.02	5.0GlyAsp: 5.0 ± 0.05	4.949GlyGlu: 4.949 ± 0.054	2.898GlyPhe: 2.898 ± 0.038	8.79GlyGly: 8.79 ± 0.084	2.54GlyHis: 2.54 ± 0.034	3.528GlyIle: 3.528 ± 0.036	2.449GlyLys: 2.449 ± 0.043	9.511GlyLeu: 9.511 ± 0.064	1.979GlyMet: 1.979 ± 0.031	1.83GlyAsn: 1.83 ± 0.03	5.25GlyPro: 5.25 ± 0.054	2.809GlyGln: 2.809 ± 0.036	7.856GlyArg: 7.856 ± 0.061	5.382GlySer: 5.382 ± 0.053	6.71GlyThr: 6.71 ± 0.073	7.368GlyVal: 7.368 ± 0.061	1.732GlyTrp: 1.732 ± 0.026	2.371GlyTyr: 2.371 ± 0.034	0.0GlyXaa: 0.0 ± 0.0
His	2.873HisAla: 2.873 ± 0.036	0.229HisCys: 0.229 ± 0.011	1.389HisAsp: 1.389 ± 0.024	1.255HisGlu: 1.255 ± 0.023	0.692HisPhe: 0.692 ± 0.018	2.595HisGly: 2.595 ± 0.031	0.748HisHis: 0.748 ± 0.019	0.738HisIle: 0.738 ± 0.017	0.396HisLys: 0.396 ± 0.014	2.516HisLeu: 2.516 ± 0.035	0.356HisMet: 0.356 ± 0.013	0.394HisAsn: 0.394 ± 0.015	1.904HisPro: 1.904 ± 0.031	0.686HisGln: 0.686 ± 0.018	2.166HisArg: 2.166 ± 0.034	1.053HisSer: 1.053 ± 0.024	1.453HisThr: 1.453 ± 0.025	1.773HisVal: 1.773 ± 0.031	0.4HisTrp: 0.4 ± 0.012	0.51HisTyr: 0.51 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	4.746IleAla: 4.746 ± 0.05	0.289IleCys: 0.289 ± 0.011	2.166IleAsp: 2.166 ± 0.03	1.89IleGlu: 1.89 ± 0.029	0.676IlePhe: 0.676 ± 0.017	3.549IleGly: 3.549 ± 0.045	0.624IleHis: 0.624 ± 0.015	0.866IleIle: 0.866 ± 0.02	0.749IleLys: 0.749 ± 0.02	2.452IleLeu: 2.452 ± 0.035	0.455IleMet: 0.455 ± 0.016	0.702IleAsn: 0.702 ± 0.018	1.785IlePro: 1.785 ± 0.028	0.781IleGln: 0.781 ± 0.017	2.281IleArg: 2.281 ± 0.033	1.661IleSer: 1.661 ± 0.03	2.286IleThr: 2.286 ± 0.034	2.669IleVal: 2.669 ± 0.036	0.386IleTrp: 0.386 ± 0.015	0.545IleTyr: 0.545 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	3.0LysAla: 3.0 ± 0.044	0.121LysCys: 0.121 ± 0.007	1.41LysAsp: 1.41 ± 0.029	1.132LysGlu: 1.132 ± 0.025	0.479LysPhe: 0.479 ± 0.016	1.856LysGly: 1.856 ± 0.034	0.43LysHis: 0.43 ± 0.015	0.891LysIle: 0.891 ± 0.023	0.868LysLys: 0.868 ± 0.026	2.023LysLeu: 2.023 ± 0.034	0.404LysMet: 0.404 ± 0.013	0.559LysAsn: 0.559 ± 0.019	1.317LysPro: 1.317 ± 0.026	0.747LysGln: 0.747 ± 0.021	1.389LysArg: 1.389 ± 0.027	1.136LysSer: 1.136 ± 0.025	1.294LysThr: 1.294 ± 0.033	1.932LysVal: 1.932 ± 0.031	0.285LysTrp: 0.285 ± 0.011	0.481LysTyr: 0.481 ± 0.017	0.0LysXaa: 0.0 ± 0.0
Leu	15.355LeuAla: 15.355 ± 0.098	0.869LeuCys: 0.869 ± 0.021	6.494LeuAsp: 6.494 ± 0.07	4.721LeuGlu: 4.721 ± 0.055	2.605LeuPhe: 2.605 ± 0.039	9.404LeuGly: 9.404 ± 0.062	2.419LeuHis: 2.419 ± 0.036	3.335LeuIle: 3.335 ± 0.044	2.151LeuLys: 2.151 ± 0.037	11.45LeuLeu: 11.45 ± 0.093	1.604LeuMet: 1.604 ± 0.024	1.712LeuAsn: 1.712 ± 0.03	6.618LeuPro: 6.618 ± 0.059	2.358LeuGln: 2.358 ± 0.031	8.561LeuArg: 8.561 ± 0.078	5.235LeuSer: 5.235 ± 0.053	6.965LeuThr: 6.965 ± 0.062	8.719LeuVal: 8.719 ± 0.071	1.359LeuTrp: 1.359 ± 0.023	1.949LeuTyr: 1.949 ± 0.028	0.0LeuXaa: 0.0 ± 0.0
Met	2.25MetAla: 2.25 ± 0.03	0.15MetCys: 0.15 ± 0.009	0.879MetAsp: 0.879 ± 0.019	0.718MetGlu: 0.718 ± 0.018	0.452MetPhe: 0.452 ± 0.013	1.277MetGly: 1.277 ± 0.026	0.363MetHis: 0.363 ± 0.013	0.67MetIle: 0.67 ± 0.017	0.42MetLys: 0.42 ± 0.016	1.654MetLeu: 1.654 ± 0.027	0.288MetMet: 0.288 ± 0.011	0.429MetAsn: 0.429 ± 0.013	1.112MetPro: 1.112 ± 0.021	0.459MetGln: 0.459 ± 0.013	1.374MetArg: 1.374 ± 0.022	1.293MetSer: 1.293 ± 0.022	1.576MetThr: 1.576 ± 0.027	1.245MetVal: 1.245 ± 0.023	0.223MetTrp: 0.223 ± 0.009	0.324MetTyr: 0.324 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.207AsnAla: 2.207 ± 0.037	0.179AsnCys: 0.179 ± 0.009	0.995AsnAsp: 0.995 ± 0.021	0.772AsnGlu: 0.772 ± 0.018	0.492AsnPhe: 0.492 ± 0.015	1.949AsnGly: 1.949 ± 0.037	0.412AsnHis: 0.412 ± 0.013	0.655AsnIle: 0.655 ± 0.019	0.414AsnLys: 0.414 ± 0.015	1.663AsnLeu: 1.663 ± 0.025	0.311AsnMet: 0.311 ± 0.012	0.483AsnAsn: 0.483 ± 0.021	1.389AsnPro: 1.389 ± 0.027	0.551AsnGln: 0.551 ± 0.018	1.242AsnArg: 1.242 ± 0.023	0.97AsnSer: 0.97 ± 0.021	1.125AsnThr: 1.125 ± 0.026	1.4AsnVal: 1.4 ± 0.027	0.322AsnTrp: 0.322 ± 0.011	0.409AsnTyr: 0.409 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	9.034ProAla: 9.034 ± 0.086	0.365ProCys: 0.365 ± 0.013	4.311ProAsp: 4.311 ± 0.052	4.095ProGlu: 4.095 ± 0.048	1.524ProPhe: 1.524 ± 0.023	6.858ProGly: 6.858 ± 0.07	1.5ProHis: 1.5 ± 0.027	1.188ProIle: 1.188 ± 0.025	1.193ProLys: 1.193 ± 0.027	5.442ProLeu: 5.442 ± 0.054	0.997ProMet: 0.997 ± 0.023	0.912ProAsn: 0.912 ± 0.021	3.522ProPro: 3.522 ± 0.059	1.967ProGln: 1.967 ± 0.039	3.953ProArg: 3.953 ± 0.049	3.097ProSer: 3.097 ± 0.041	3.011ProThr: 3.011 ± 0.044	5.589ProVal: 5.589 ± 0.047	0.928ProTrp: 0.928 ± 0.022	1.5ProTyr: 1.5 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	4.092GlnAla: 4.092 ± 0.046	0.195GlnCys: 0.195 ± 0.009	1.508GlnAsp: 1.508 ± 0.029	1.493GlnGlu: 1.493 ± 0.028	0.74GlnPhe: 0.74 ± 0.018	2.48GlnGly: 2.48 ± 0.035	0.744GlnHis: 0.744 ± 0.018	1.12GlnIle: 1.12 ± 0.02	0.7GlnLys: 0.7 ± 0.017	3.217GlnLeu: 3.217 ± 0.039	0.513GlnMet: 0.513 ± 0.016	0.541GlnAsn: 0.541 ± 0.017	1.821GlnPro: 1.821 ± 0.045	1.395GlnGln: 1.395 ± 0.037	2.393GlnArg: 2.393 ± 0.032	1.368GlnSer: 1.368 ± 0.023	1.454GlnThr: 1.454 ± 0.026	2.463GlnVal: 2.463 ± 0.035	0.514GlnTrp: 0.514 ± 0.017	0.668GlnTyr: 0.668 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	10.106ArgAla: 10.106 ± 0.082	0.606ArgCys: 0.606 ± 0.018	4.075ArgAsp: 4.075 ± 0.049	4.609ArgGlu: 4.609 ± 0.051	2.318ArgPhe: 2.318 ± 0.032	5.668ArgGly: 5.668 ± 0.048	2.204ArgHis: 2.204 ± 0.033	3.274ArgIle: 3.274 ± 0.041	1.581ArgLys: 1.581 ± 0.026	8.905ArgLeu: 8.905 ± 0.084	1.669ArgMet: 1.669 ± 0.027	1.318ArgAsn: 1.318 ± 0.023	4.993ArgPro: 4.993 ± 0.056	2.441ArgGln: 2.441 ± 0.03	7.797ArgArg: 7.797 ± 0.075	3.941ArgSer: 3.941 ± 0.042	5.398ArgThr: 5.398 ± 0.048	5.627ArgVal: 5.627 ± 0.061	1.382ArgTrp: 1.382 ± 0.023	1.821ArgTyr: 1.821 ± 0.027	0.0ArgXaa: 0.0 ± 0.0
Ser	6.971SerAla: 6.971 ± 0.072	0.423SerCys: 0.423 ± 0.014	2.534SerAsp: 2.534 ± 0.037	2.228SerGlu: 2.228 ± 0.037	1.516SerPhe: 1.516 ± 0.028	6.032SerGly: 6.032 ± 0.062	1.053SerHis: 1.053 ± 0.02	1.375SerIle: 1.375 ± 0.023	1.002SerLys: 1.002 ± 0.027	4.898SerLeu: 4.898 ± 0.043	1.05SerMet: 1.05 ± 0.019	0.895SerAsn: 0.895 ± 0.022	3.101SerPro: 3.101 ± 0.035	1.306SerGln: 1.306 ± 0.026	3.559SerArg: 3.559 ± 0.041	2.908SerSer: 2.908 ± 0.045	3.042SerThr: 3.042 ± 0.043	4.223SerVal: 4.223 ± 0.04	0.907SerTrp: 0.907 ± 0.02	1.266SerTyr: 1.266 ± 0.024	0.0SerXaa: 0.0 ± 0.0
Thr	9.101ThrAla: 9.101 ± 0.069	0.46ThrCys: 0.46 ± 0.013	3.632ThrAsp: 3.632 ± 0.042	3.097ThrGlu: 3.097 ± 0.039	1.642ThrPhe: 1.642 ± 0.028	6.963ThrGly: 6.963 ± 0.061	1.279ThrHis: 1.279 ± 0.025	1.725ThrIle: 1.725 ± 0.03	1.194ThrLys: 1.194 ± 0.025	5.779ThrLeu: 5.779 ± 0.049	0.924ThrMet: 0.924 ± 0.019	1.033ThrAsn: 1.033 ± 0.023	4.134ThrPro: 4.134 ± 0.048	1.466ThrGln: 1.466 ± 0.028	3.895ThrArg: 3.895 ± 0.04	3.202ThrSer: 3.202 ± 0.046	3.905ThrThr: 3.905 ± 0.064	6.128ThrVal: 6.128 ± 0.052	0.937ThrTrp: 0.937 ± 0.021	1.377ThrTyr: 1.377 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	10.468ValAla: 10.468 ± 0.078	0.799ValCys: 0.799 ± 0.017	4.577ValAsp: 4.577 ± 0.041	4.398ValGlu: 4.398 ± 0.04	2.465ValPhe: 2.465 ± 0.036	6.237ValGly: 6.237 ± 0.059	2.052ValHis: 2.052 ± 0.033	2.866ValIle: 2.866 ± 0.037	1.756ValLys: 1.756 ± 0.033	9.583ValLeu: 9.583 ± 0.073	1.418ValMet: 1.418 ± 0.028	1.707ValAsn: 1.707 ± 0.03	5.352ValPro: 5.352 ± 0.047	2.243ValGln: 2.243 ± 0.033	7.191ValArg: 7.191 ± 0.067	4.329ValSer: 4.329 ± 0.047	5.758ValThr: 5.758 ± 0.048	7.743ValVal: 7.743 ± 0.08	1.152ValTrp: 1.152 ± 0.022	1.621ValTyr: 1.621 ± 0.027	0.0ValXaa: 0.0 ± 0.0
Trp	1.713TrpAla: 1.713 ± 0.031	0.148TrpCys: 0.148 ± 0.007	0.825TrpAsp: 0.825 ± 0.018	0.696TrpGlu: 0.696 ± 0.017	0.503TrpPhe: 0.503 ± 0.016	1.079TrpGly: 1.079 ± 0.022	0.407TrpHis: 0.407 ± 0.013	0.581TrpIle: 0.581 ± 0.015	0.38TrpLys: 0.38 ± 0.013	1.845TrpLeu: 1.845 ± 0.033	0.277TrpMet: 0.277 ± 0.012	0.427TrpAsn: 0.427 ± 0.014	0.812TrpPro: 0.812 ± 0.021	0.666TrpGln: 0.666 ± 0.018	1.329TrpArg: 1.329 ± 0.023	0.956TrpSer: 0.956 ± 0.022	1.069TrpThr: 1.069 ± 0.021	0.997TrpVal: 0.997 ± 0.022	0.362TrpTrp: 0.362 ± 0.015	0.382TrpTyr: 0.382 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.863TyrAla: 2.863 ± 0.034	0.187TyrCys: 0.187 ± 0.009	1.651TyrAsp: 1.651 ± 0.032	1.213TyrGlu: 1.213 ± 0.025	0.682TyrPhe: 0.682 ± 0.016	2.385TyrGly: 2.385 ± 0.035	0.417TyrHis: 0.417 ± 0.014	0.486TyrIle: 0.486 ± 0.017	0.434TyrLys: 0.434 ± 0.015	2.09TyrLeu: 2.09 ± 0.035	0.246TyrMet: 0.246 ± 0.011	0.426TyrAsn: 0.426 ± 0.014	1.112TyrPro: 1.112 ± 0.023	0.67TyrGln: 0.67 ± 0.018	1.796TyrArg: 1.796 ± 0.029	0.985TyrSer: 0.985 ± 0.021	1.279TyrThr: 1.279 ± 0.029	1.68TyrVal: 1.68 ± 0.028	0.356TyrTrp: 0.356 ± 0.013	0.484TyrTyr: 0.484 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7600 proteins (2447124 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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