Pelargonium ringspot virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Pelarspovirus

Average proteome isoelectric point is 8.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q911J5|Q911J5_9TOMB Capsid protein OS=Pelargonium ringspot virus OX=167020 GN=p37 PE=3 SV=1
MM1 pKa = 7.4TDD3 pKa = 3.07VVKK6 pKa = 10.78FGVWAGFYY14 pKa = 9.96LAKK17 pKa = 10.25EE18 pKa = 3.99LALLGPEE25 pKa = 4.33LASITLSSLTPNYY38 pKa = 10.49NGLLTSIGLPTVSSIPTASPPIDD61 pKa = 3.23LVLVNDD67 pKa = 4.83GVLNTPNLEE76 pKa = 4.24LEE78 pKa = 4.61GEE80 pKa = 4.25LVPLLEE86 pKa = 5.17EE87 pKa = 4.85VITDD91 pKa = 4.26PKK93 pKa = 10.43TGTITKK99 pKa = 9.61AVKK102 pKa = 8.99TRR104 pKa = 11.84RR105 pKa = 11.84PKK107 pKa = 10.73SKK109 pKa = 7.87TTFSCILAAEE119 pKa = 4.56TKK121 pKa = 10.1NHH123 pKa = 6.92FGGLPRR129 pKa = 11.84ATRR132 pKa = 11.84ANEE135 pKa = 3.79LSVMKK140 pKa = 10.53HH141 pKa = 4.93LVNRR145 pKa = 11.84CKK147 pKa = 10.4EE148 pKa = 4.28CKK150 pKa = 9.08LTALQTRR157 pKa = 11.84EE158 pKa = 3.76VSAKK162 pKa = 10.3AFSLVFTPDD171 pKa = 2.78SHH173 pKa = 8.07DD174 pKa = 3.34KK175 pKa = 10.89FIYY178 pKa = 10.27EE179 pKa = 4.59FLNSDD184 pKa = 3.01ITFEE188 pKa = 4.53RR189 pKa = 11.84RR190 pKa = 11.84CDD192 pKa = 3.57YY193 pKa = 11.04LKK195 pKa = 10.56SQRR198 pKa = 11.84VDD200 pKa = 3.55SCWLRR205 pKa = 11.84LLQNPFGKK213 pKa = 10.16KK214 pKa = 6.4RR215 pKa = 11.84WKK217 pKa = 10.72AVVCRR222 pKa = 11.84LMGMGVQEE230 pKa = 4.21AYY232 pKa = 10.38EE233 pKa = 4.07FVKK236 pKa = 10.97

Molecular weight: 26.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q911J5|Q911J5_9TOMB Capsid protein OS=Pelargonium ringspot virus OX=167020 GN=p37 PE=3 SV=1
MM1 pKa = 7.74ASNSPIVLAAANRR14 pKa = 11.84GEE16 pKa = 4.14VWAVKK21 pKa = 10.24LKK23 pKa = 9.99QSGWKK28 pKa = 8.19TLSKK32 pKa = 10.15AQKK35 pKa = 9.73AAARR39 pKa = 11.84AQGIGAAPLSQTVPKK54 pKa = 7.67TTRR57 pKa = 11.84IIQGNPTFSRR67 pKa = 11.84RR68 pKa = 11.84SRR70 pKa = 11.84DD71 pKa = 3.43APGNAQKK78 pKa = 11.43SMTIVKK84 pKa = 10.09SEE86 pKa = 3.84FLGEE90 pKa = 4.02VSADD94 pKa = 3.9GAIHH98 pKa = 7.34TYY100 pKa = 9.78TLDD103 pKa = 3.45PRR105 pKa = 11.84NVRR108 pKa = 11.84TFPHH112 pKa = 6.46ISDD115 pKa = 4.47LARR118 pKa = 11.84GFNKK122 pKa = 10.4YY123 pKa = 10.36KK124 pKa = 10.77FGDD127 pKa = 3.53LKK129 pKa = 10.95VRR131 pKa = 11.84YY132 pKa = 8.35SAKK135 pKa = 10.08VQEE138 pKa = 4.74SGCGVCIAFTPDD150 pKa = 3.13SSDD153 pKa = 3.5PKK155 pKa = 10.59PKK157 pKa = 10.71GKK159 pKa = 10.15FDD161 pKa = 4.29LYY163 pKa = 11.22SLGSRR168 pKa = 11.84AEE170 pKa = 4.28GAAHH174 pKa = 7.36RR175 pKa = 11.84NLLYY179 pKa = 9.88TVPIDD184 pKa = 3.19KK185 pKa = 10.43SQRR188 pKa = 11.84FLRR191 pKa = 11.84DD192 pKa = 3.05CATEE196 pKa = 3.8NSKK199 pKa = 11.2DD200 pKa = 3.35VDD202 pKa = 3.72AGSLFVLVDD211 pKa = 3.72GQHH214 pKa = 6.8DD215 pKa = 3.99GRR217 pKa = 11.84VGEE220 pKa = 4.86LFLEE224 pKa = 4.5VTIVLSQPTYY234 pKa = 9.22NQRR237 pKa = 11.84ATQLLAGTTHH247 pKa = 7.3RR248 pKa = 11.84DD249 pKa = 2.95GPTFVKK255 pKa = 10.33AVKK258 pKa = 9.91SANTVSLTFQAAGNYY273 pKa = 8.89LVSTYY278 pKa = 11.09SSLLEE283 pKa = 3.98RR284 pKa = 11.84VGRR287 pKa = 11.84LALGEE292 pKa = 4.34AEE294 pKa = 4.09QSQVDD299 pKa = 4.06SSTHH303 pKa = 5.24SSNVVEE309 pKa = 4.83ALVAAPGGSIVYY321 pKa = 8.83YY322 pKa = 10.45FKK324 pKa = 10.83TPDD327 pKa = 3.2QMSFRR332 pKa = 11.84AYY334 pKa = 8.46VCRR337 pKa = 11.84MM338 pKa = 3.31

Molecular weight: 36.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1477

58

762

295.4

32.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.703 ± 1.292

1.828 ± 0.315

4.333 ± 0.316

5.213 ± 0.909

4.333 ± 0.269

6.838 ± 0.421

2.099 ± 0.481

4.265 ± 0.448

6.838 ± 0.415

10.088 ± 1.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.828 ± 0.291

3.995 ± 0.181

5.146 ± 0.273

2.911 ± 0.592

5.619 ± 0.734

8.125 ± 1.485

6.5 ± 0.54

8.734 ± 0.43

1.219 ± 0.232

3.318 ± 0.358
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski