Methanocaldococcus fervens tailed virus 1

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified archaeal dsDNA viruses

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G9VYS6|A0A7G9VYS6_9VIRU Uncharacterized protein OS=Methanocaldococcus fervens tailed virus 1 OX=2759191 PE=4 SV=1
MM1 pKa = 7.69PAGPEE6 pKa = 3.86QSQSLVAKK14 pKa = 10.27INQLVLLGKK23 pKa = 9.06GTFVYY28 pKa = 10.84CEE30 pKa = 4.21FDD32 pKa = 3.56SQATEE37 pKa = 3.67SDD39 pKa = 3.29KK40 pKa = 11.64ALTIVDD46 pKa = 3.2NTIRR50 pKa = 11.84FNLPPNYY57 pKa = 10.1YY58 pKa = 9.37PVIVDD63 pKa = 3.24IVAVDD68 pKa = 3.46EE69 pKa = 4.83NGNEE73 pKa = 3.97IYY75 pKa = 10.68GIPISKK81 pKa = 8.24ITYY84 pKa = 9.96DD85 pKa = 4.0YY86 pKa = 11.81DD87 pKa = 3.61EE88 pKa = 5.43LPQGNRR94 pKa = 11.84QVIAEE99 pKa = 4.1PSEE102 pKa = 3.87NNTIDD107 pKa = 4.29EE108 pKa = 4.4SKK110 pKa = 10.21HH111 pKa = 4.49WIAYY115 pKa = 6.48VVCIGIDD122 pKa = 3.44DD123 pKa = 4.23TKK125 pKa = 10.87PIVDD129 pKa = 3.95VKK131 pKa = 10.27SQSGSVEE138 pKa = 3.9PKK140 pKa = 10.21GRR142 pKa = 11.84IYY144 pKa = 10.54LINYY148 pKa = 9.06SDD150 pKa = 3.74TNGTDD155 pKa = 3.48EE156 pKa = 4.74ITIQYY161 pKa = 10.28FNGTSEE167 pKa = 4.5TITVDD172 pKa = 3.98KK173 pKa = 11.35ANLWTVNPYY182 pKa = 9.12TLGG185 pKa = 3.58

Molecular weight: 20.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G9VYT4|A0A7G9VYT4_9VIRU Histone domain-containing protein OS=Methanocaldococcus fervens tailed virus 1 OX=2759191 PE=4 SV=1
MM1 pKa = 7.42QILAFSKK8 pKa = 10.65QEE10 pKa = 3.74NGRR13 pKa = 11.84IKK15 pKa = 9.1QTYY18 pKa = 8.54VIEE21 pKa = 4.29NNGRR25 pKa = 11.84KK26 pKa = 9.56SFFGVIKK33 pKa = 10.73GRR35 pKa = 11.84GDD37 pKa = 2.96KK38 pKa = 10.56RR39 pKa = 11.84GRR41 pKa = 11.84DD42 pKa = 3.53YY43 pKa = 11.63GFKK46 pKa = 10.43RR47 pKa = 3.6

Molecular weight: 5.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

9374

41

846

218.0

24.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.505 ± 0.504

0.949 ± 0.158

6.07 ± 0.198

8.257 ± 0.355

3.766 ± 0.244

5.355 ± 0.322

1.365 ± 0.11

9.494 ± 0.36

10.188 ± 0.531

9.26 ± 0.58

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.251 ± 0.174

5.473 ± 0.302

3.104 ± 0.259

3.008 ± 0.285

3.808 ± 0.359

5.323 ± 0.293

5.003 ± 0.426

6.443 ± 0.427

1.035 ± 0.102

4.342 ± 0.409
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski