Amino acid dipepetide frequency for Klebsiella phage Kp2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.865AlaAla: 15.865 ± 1.459
0.507AlaCys: 0.507 ± 0.201
6.52AlaAsp: 6.52 ± 0.739
5.433AlaGlu: 5.433 ± 0.669
3.187AlaPhe: 3.187 ± 0.406
7.606AlaGly: 7.606 ± 1.054
1.014AlaHis: 1.014 ± 0.299
4.636AlaIle: 4.636 ± 0.696
5.868AlaLys: 5.868 ± 1.012
9.49AlaLeu: 9.49 ± 0.833
2.753AlaMet: 2.753 ± 0.443
3.043AlaAsn: 3.043 ± 0.438
4.274AlaPro: 4.274 ± 0.899
5.433AlaGln: 5.433 ± 0.886
5.433AlaArg: 5.433 ± 0.718
6.375AlaSer: 6.375 ± 0.769
5.433AlaThr: 5.433 ± 0.915
6.882AlaVal: 6.882 ± 0.857
1.087AlaTrp: 1.087 ± 0.34
3.767AlaTyr: 3.767 ± 0.571
0.0AlaXaa: 0.0 ± 0.0
Cys
0.58CysAla: 0.58 ± 0.228
0.362CysCys: 0.362 ± 0.228
0.652CysAsp: 0.652 ± 0.188
0.435CysGlu: 0.435 ± 0.178
0.362CysPhe: 0.362 ± 0.163
0.58CysGly: 0.58 ± 0.192
0.435CysHis: 0.435 ± 0.213
0.29CysIle: 0.29 ± 0.142
0.435CysLys: 0.435 ± 0.211
0.942CysLeu: 0.942 ± 0.284
0.58CysMet: 0.58 ± 0.179
0.435CysAsn: 0.435 ± 0.172
0.797CysPro: 0.797 ± 0.302
0.217CysGln: 0.217 ± 0.127
0.652CysArg: 0.652 ± 0.241
0.942CysSer: 0.942 ± 0.257
1.159CysThr: 1.159 ± 0.272
0.724CysVal: 0.724 ± 0.225
0.29CysTrp: 0.29 ± 0.148
0.58CysTyr: 0.58 ± 0.2
0.0CysXaa: 0.0 ± 0.0
Asp
7.462AspAla: 7.462 ± 0.849
1.232AspCys: 1.232 ± 0.368
3.26AspAsp: 3.26 ± 0.539
3.839AspGlu: 3.839 ± 0.51
2.246AspPhe: 2.246 ± 0.353
4.781AspGly: 4.781 ± 0.595
0.29AspHis: 0.29 ± 0.183
3.187AspIle: 3.187 ± 0.452
2.825AspLys: 2.825 ± 0.495
5.361AspLeu: 5.361 ± 0.517
2.318AspMet: 2.318 ± 0.401
2.898AspAsn: 2.898 ± 0.494
3.043AspPro: 3.043 ± 0.479
1.594AspGln: 1.594 ± 0.281
2.535AspArg: 2.535 ± 0.561
4.999AspSer: 4.999 ± 0.475
3.984AspThr: 3.984 ± 0.538
4.274AspVal: 4.274 ± 0.433
0.797AspTrp: 0.797 ± 0.183
2.391AspTyr: 2.391 ± 0.424
0.0AspXaa: 0.0 ± 0.0
Glu
5.723GluAla: 5.723 ± 0.833
0.29GluCys: 0.29 ± 0.142
2.825GluAsp: 2.825 ± 0.395
3.695GluGlu: 3.695 ± 0.933
2.463GluPhe: 2.463 ± 0.397
3.767GluGly: 3.767 ± 0.673
2.535GluHis: 2.535 ± 0.419
2.246GluIle: 2.246 ± 0.464
2.101GluLys: 2.101 ± 0.382
5.288GluLeu: 5.288 ± 0.602
2.028GluMet: 2.028 ± 0.345
1.594GluAsn: 1.594 ± 0.352
1.521GluPro: 1.521 ± 0.278
3.55GluGln: 3.55 ± 0.724
3.984GluArg: 3.984 ± 0.554
3.115GluSer: 3.115 ± 0.52
2.898GluThr: 2.898 ± 0.434
5.216GluVal: 5.216 ± 0.482
1.087GluTrp: 1.087 ± 0.231
2.898GluTyr: 2.898 ± 0.487
0.0GluXaa: 0.0 ± 0.0
Phe
2.68PheAla: 2.68 ± 0.35
0.58PheCys: 0.58 ± 0.267
2.173PheAsp: 2.173 ± 0.323
2.028PheGlu: 2.028 ± 0.388
1.232PhePhe: 1.232 ± 0.269
2.101PheGly: 2.101 ± 0.363
0.362PheHis: 0.362 ± 0.159
1.449PheIle: 1.449 ± 0.297
2.246PheLys: 2.246 ± 0.419
2.101PheLeu: 2.101 ± 0.358
0.652PheMet: 0.652 ± 0.25
1.449PheAsn: 1.449 ± 0.314
1.449PhePro: 1.449 ± 0.26
1.594PheGln: 1.594 ± 0.25
1.739PheArg: 1.739 ± 0.398
1.594PheSer: 1.594 ± 0.338
2.246PheThr: 2.246 ± 0.451
1.739PheVal: 1.739 ± 0.471
0.58PheTrp: 0.58 ± 0.186
1.376PheTyr: 1.376 ± 0.27
0.0PheXaa: 0.0 ± 0.0
Gly
5.868GlyAla: 5.868 ± 0.554
1.376GlyCys: 1.376 ± 0.318
4.999GlyAsp: 4.999 ± 0.563
4.347GlyGlu: 4.347 ± 0.424
2.391GlyPhe: 2.391 ± 0.434
4.564GlyGly: 4.564 ± 0.637
1.087GlyHis: 1.087 ± 0.262
3.912GlyIle: 3.912 ± 0.63
3.767GlyLys: 3.767 ± 0.563
6.303GlyLeu: 6.303 ± 0.741
1.956GlyMet: 1.956 ± 0.512
3.622GlyAsn: 3.622 ± 0.578
1.666GlyPro: 1.666 ± 0.358
2.97GlyGln: 2.97 ± 0.526
4.854GlyArg: 4.854 ± 0.479
6.23GlySer: 6.23 ± 0.763
4.564GlyThr: 4.564 ± 0.743
5.795GlyVal: 5.795 ± 0.562
0.58GlyTrp: 0.58 ± 0.193
2.97GlyTyr: 2.97 ± 0.57
0.0GlyXaa: 0.0 ± 0.0
His
1.232HisAla: 1.232 ± 0.363
0.217HisCys: 0.217 ± 0.109
1.087HisAsp: 1.087 ± 0.263
1.159HisGlu: 1.159 ± 0.353
0.29HisPhe: 0.29 ± 0.125
1.594HisGly: 1.594 ± 0.415
0.072HisHis: 0.072 ± 0.071
0.869HisIle: 0.869 ± 0.22
0.869HisLys: 0.869 ± 0.225
1.956HisLeu: 1.956 ± 0.398
0.435HisMet: 0.435 ± 0.153
0.652HisAsn: 0.652 ± 0.243
0.724HisPro: 0.724 ± 0.328
0.507HisGln: 0.507 ± 0.196
1.304HisArg: 1.304 ± 0.306
0.869HisSer: 0.869 ± 0.249
0.724HisThr: 0.724 ± 0.225
0.797HisVal: 0.797 ± 0.281
0.29HisTrp: 0.29 ± 0.144
0.652HisTyr: 0.652 ± 0.275
0.0HisXaa: 0.0 ± 0.0
Ile
2.97IleAla: 2.97 ± 0.478
0.58IleCys: 0.58 ± 0.205
3.115IleAsp: 3.115 ± 0.372
2.68IleGlu: 2.68 ± 0.462
0.724IlePhe: 0.724 ± 0.188
2.391IleGly: 2.391 ± 0.352
0.652IleHis: 0.652 ± 0.201
1.956IleIle: 1.956 ± 0.34
3.332IleLys: 3.332 ± 0.606
4.564IleLeu: 4.564 ± 0.493
1.449IleMet: 1.449 ± 0.26
1.594IleAsn: 1.594 ± 0.297
2.101IlePro: 2.101 ± 0.475
2.97IleGln: 2.97 ± 0.397
2.68IleArg: 2.68 ± 0.377
3.115IleSer: 3.115 ± 0.573
2.753IleThr: 2.753 ± 0.518
2.753IleVal: 2.753 ± 0.417
0.29IleTrp: 0.29 ± 0.145
1.449IleTyr: 1.449 ± 0.347
0.0IleXaa: 0.0 ± 0.0
Lys
6.158LysAla: 6.158 ± 0.784
0.58LysCys: 0.58 ± 0.21
2.825LysAsp: 2.825 ± 0.355
3.26LysGlu: 3.26 ± 0.576
1.304LysPhe: 1.304 ± 0.334
3.26LysGly: 3.26 ± 0.495
0.724LysHis: 0.724 ± 0.237
1.666LysIle: 1.666 ± 0.365
1.956LysLys: 1.956 ± 0.411
4.926LysLeu: 4.926 ± 0.669
1.376LysMet: 1.376 ± 0.295
1.087LysAsn: 1.087 ± 0.238
1.376LysPro: 1.376 ± 0.289
3.695LysGln: 3.695 ± 0.502
3.26LysArg: 3.26 ± 0.495
2.753LysSer: 2.753 ± 0.46
2.898LysThr: 2.898 ± 0.383
3.043LysVal: 3.043 ± 0.481
0.942LysTrp: 0.942 ± 0.23
1.449LysTyr: 1.449 ± 0.332
0.0LysXaa: 0.0 ± 0.0
Leu
8.041LeuAla: 8.041 ± 0.818
1.232LeuCys: 1.232 ± 0.302
6.665LeuAsp: 6.665 ± 0.736
5.723LeuGlu: 5.723 ± 0.508
2.463LeuPhe: 2.463 ± 0.313
7.244LeuGly: 7.244 ± 0.829
1.304LeuHis: 1.304 ± 0.284
4.274LeuIle: 4.274 ± 0.737
3.115LeuLys: 3.115 ± 0.468
7.099LeuLeu: 7.099 ± 0.746
1.884LeuMet: 1.884 ± 0.373
3.332LeuAsn: 3.332 ± 0.502
3.332LeuPro: 3.332 ± 0.484
4.202LeuGln: 4.202 ± 0.432
5.94LeuArg: 5.94 ± 0.581
5.071LeuSer: 5.071 ± 0.705
5.216LeuThr: 5.216 ± 0.58
6.303LeuVal: 6.303 ± 0.726
1.449LeuTrp: 1.449 ± 0.315
3.332LeuTyr: 3.332 ± 0.388
0.0LeuXaa: 0.0 ± 0.0
Met
3.839MetAla: 3.839 ± 0.633
0.145MetCys: 0.145 ± 0.09
1.956MetAsp: 1.956 ± 0.494
1.232MetGlu: 1.232 ± 0.255
1.014MetPhe: 1.014 ± 0.338
1.376MetGly: 1.376 ± 0.244
0.724MetHis: 0.724 ± 0.25
0.652MetIle: 0.652 ± 0.211
0.869MetLys: 0.869 ± 0.283
3.332MetLeu: 3.332 ± 0.482
0.652MetMet: 0.652 ± 0.267
0.942MetAsn: 0.942 ± 0.288
1.232MetPro: 1.232 ± 0.273
2.028MetGln: 2.028 ± 0.405
2.028MetArg: 2.028 ± 0.536
2.463MetSer: 2.463 ± 0.479
1.087MetThr: 1.087 ± 0.265
1.884MetVal: 1.884 ± 0.406
0.362MetTrp: 0.362 ± 0.153
1.159MetTyr: 1.159 ± 0.34
0.0MetXaa: 0.0 ± 0.0
Asn
3.332AsnAla: 3.332 ± 0.421
0.145AsnCys: 0.145 ± 0.093
2.391AsnAsp: 2.391 ± 0.422
1.521AsnGlu: 1.521 ± 0.397
0.724AsnPhe: 0.724 ± 0.226
3.043AsnGly: 3.043 ± 0.454
0.362AsnHis: 0.362 ± 0.175
2.535AsnIle: 2.535 ± 0.434
2.101AsnLys: 2.101 ± 0.327
2.753AsnLeu: 2.753 ± 0.496
1.087AsnMet: 1.087 ± 0.331
1.449AsnAsn: 1.449 ± 0.325
2.101AsnPro: 2.101 ± 0.39
1.594AsnGln: 1.594 ± 0.347
1.666AsnArg: 1.666 ± 0.294
3.043AsnSer: 3.043 ± 0.415
2.97AsnThr: 2.97 ± 0.429
2.97AsnVal: 2.97 ± 0.39
0.58AsnTrp: 0.58 ± 0.213
1.739AsnTyr: 1.739 ± 0.338
0.0AsnXaa: 0.0 ± 0.0
Pro
4.274ProAla: 4.274 ± 0.837
0.29ProCys: 0.29 ± 0.141
2.535ProAsp: 2.535 ± 0.479
3.405ProGlu: 3.405 ± 0.474
0.869ProPhe: 0.869 ± 0.205
2.535ProGly: 2.535 ± 0.5
0.507ProHis: 0.507 ± 0.183
2.028ProIle: 2.028 ± 0.367
1.666ProLys: 1.666 ± 0.411
2.97ProLeu: 2.97 ± 0.424
1.159ProMet: 1.159 ± 0.238
1.811ProAsn: 1.811 ± 0.368
0.797ProPro: 0.797 ± 0.274
1.304ProGln: 1.304 ± 0.258
1.594ProArg: 1.594 ± 0.32
2.391ProSer: 2.391 ± 0.516
2.463ProThr: 2.463 ± 0.452
3.043ProVal: 3.043 ± 0.389
0.652ProTrp: 0.652 ± 0.205
1.521ProTyr: 1.521 ± 0.366
0.0ProXaa: 0.0 ± 0.0
Gln
4.999GlnAla: 4.999 ± 0.74
0.362GlnCys: 0.362 ± 0.187
3.26GlnAsp: 3.26 ± 0.483
3.767GlnGlu: 3.767 ± 0.561
1.811GlnPhe: 1.811 ± 0.362
3.26GlnGly: 3.26 ± 0.443
1.304GlnHis: 1.304 ± 0.307
1.087GlnIle: 1.087 ± 0.303
2.753GlnLys: 2.753 ± 0.463
4.636GlnLeu: 4.636 ± 0.55
1.232GlnMet: 1.232 ± 0.241
2.101GlnAsn: 2.101 ± 0.459
1.521GlnPro: 1.521 ± 0.417
2.97GlnGln: 2.97 ± 0.604
2.825GlnArg: 2.825 ± 0.403
3.043GlnSer: 3.043 ± 0.488
1.884GlnThr: 1.884 ± 0.405
2.898GlnVal: 2.898 ± 0.41
0.724GlnTrp: 0.724 ± 0.276
2.246GlnTyr: 2.246 ± 0.434
0.0GlnXaa: 0.0 ± 0.0
Arg
6.592ArgAla: 6.592 ± 1.114
0.507ArgCys: 0.507 ± 0.232
3.695ArgAsp: 3.695 ± 0.568
3.695ArgGlu: 3.695 ± 0.521
2.101ArgPhe: 2.101 ± 0.321
4.202ArgGly: 4.202 ± 0.731
0.942ArgHis: 0.942 ± 0.21
2.825ArgIle: 2.825 ± 0.586
3.115ArgLys: 3.115 ± 0.63
5.071ArgLeu: 5.071 ± 0.571
1.884ArgMet: 1.884 ± 0.444
2.101ArgAsn: 2.101 ± 0.414
1.594ArgPro: 1.594 ± 0.344
2.608ArgGln: 2.608 ± 0.348
4.057ArgArg: 4.057 ± 0.699
2.898ArgSer: 2.898 ± 0.556
3.26ArgThr: 3.26 ± 0.376
3.912ArgVal: 3.912 ± 0.437
1.014ArgTrp: 1.014 ± 0.246
2.028ArgTyr: 2.028 ± 0.27
0.0ArgXaa: 0.0 ± 0.0
Ser
8.766SerAla: 8.766 ± 0.89
0.724SerCys: 0.724 ± 0.216
4.347SerAsp: 4.347 ± 0.612
2.825SerGlu: 2.825 ± 0.479
2.463SerPhe: 2.463 ± 0.442
6.23SerGly: 6.23 ± 0.764
0.507SerHis: 0.507 ± 0.179
3.26SerIle: 3.26 ± 0.678
3.767SerLys: 3.767 ± 0.519
4.564SerLeu: 4.564 ± 0.604
2.608SerMet: 2.608 ± 0.405
2.535SerAsn: 2.535 ± 0.477
2.825SerPro: 2.825 ± 0.416
1.884SerGln: 1.884 ± 0.368
2.97SerArg: 2.97 ± 0.318
2.825SerSer: 2.825 ± 0.51
4.202SerThr: 4.202 ± 0.491
4.781SerVal: 4.781 ± 0.587
0.942SerTrp: 0.942 ± 0.242
1.884SerTyr: 1.884 ± 0.412
0.0SerXaa: 0.0 ± 0.0
Thr
5.795ThrAla: 5.795 ± 0.833
0.652ThrCys: 0.652 ± 0.224
3.115ThrAsp: 3.115 ± 0.482
3.115ThrGlu: 3.115 ± 0.592
2.246ThrPhe: 2.246 ± 0.384
5.578ThrGly: 5.578 ± 0.891
1.014ThrHis: 1.014 ± 0.27
2.391ThrIle: 2.391 ± 0.478
2.463ThrLys: 2.463 ± 0.422
4.926ThrLeu: 4.926 ± 0.604
1.739ThrMet: 1.739 ± 0.359
1.884ThrAsn: 1.884 ± 0.443
2.898ThrPro: 2.898 ± 0.321
2.898ThrGln: 2.898 ± 0.337
2.898ThrArg: 2.898 ± 0.525
4.347ThrSer: 4.347 ± 0.656
3.767ThrThr: 3.767 ± 0.728
4.564ThrVal: 4.564 ± 0.634
0.942ThrTrp: 0.942 ± 0.211
2.68ThrTyr: 2.68 ± 0.423
0.0ThrXaa: 0.0 ± 0.0
Val
6.447ValAla: 6.447 ± 0.859
0.652ValCys: 0.652 ± 0.207
4.419ValAsp: 4.419 ± 0.437
3.767ValGlu: 3.767 ± 0.597
1.304ValPhe: 1.304 ± 0.274
6.303ValGly: 6.303 ± 0.589
1.811ValHis: 1.811 ± 0.345
2.173ValIle: 2.173 ± 0.359
2.97ValLys: 2.97 ± 0.609
5.94ValLeu: 5.94 ± 0.83
1.811ValMet: 1.811 ± 0.339
2.97ValAsn: 2.97 ± 0.532
3.043ValPro: 3.043 ± 0.455
4.129ValGln: 4.129 ± 0.716
4.274ValArg: 4.274 ± 0.525
4.999ValSer: 4.999 ± 0.654
4.491ValThr: 4.491 ± 0.622
5.578ValVal: 5.578 ± 0.486
1.014ValTrp: 1.014 ± 0.325
2.608ValTyr: 2.608 ± 0.387
0.0ValXaa: 0.0 ± 0.0
Trp
1.014TrpAla: 1.014 ± 0.236
0.29TrpCys: 0.29 ± 0.14
0.869TrpAsp: 0.869 ± 0.207
1.159TrpGlu: 1.159 ± 0.333
0.869TrpPhe: 0.869 ± 0.29
0.652TrpGly: 0.652 ± 0.246
0.217TrpHis: 0.217 ± 0.115
0.652TrpIle: 0.652 ± 0.231
0.362TrpLys: 0.362 ± 0.143
1.232TrpLeu: 1.232 ± 0.244
0.362TrpMet: 0.362 ± 0.196
0.724TrpAsn: 0.724 ± 0.238
0.362TrpPro: 0.362 ± 0.175
0.435TrpGln: 0.435 ± 0.186
1.014TrpArg: 1.014 ± 0.238
0.797TrpSer: 0.797 ± 0.258
1.087TrpThr: 1.087 ± 0.185
1.304TrpVal: 1.304 ± 0.27
0.362TrpTrp: 0.362 ± 0.155
0.869TrpTyr: 0.869 ± 0.243
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.97TyrAla: 2.97 ± 0.507
0.724TyrCys: 0.724 ± 0.26
2.68TyrAsp: 2.68 ± 0.414
1.739TyrGlu: 1.739 ± 0.459
1.304TyrPhe: 1.304 ± 0.254
2.753TyrGly: 2.753 ± 0.577
0.435TyrHis: 0.435 ± 0.18
2.028TyrIle: 2.028 ± 0.54
1.956TyrLys: 1.956 ± 0.326
3.767TyrLeu: 3.767 ± 0.52
0.942TyrMet: 0.942 ± 0.253
1.884TyrAsn: 1.884 ± 0.351
1.159TyrPro: 1.159 ± 0.216
2.101TyrGln: 2.101 ± 0.401
2.391TyrArg: 2.391 ± 0.493
3.043TyrSer: 3.043 ± 0.491
2.825TyrThr: 2.825 ± 0.471
2.246TyrVal: 2.246 ± 0.481
0.652TyrTrp: 0.652 ± 0.221
1.014TyrTyr: 1.014 ± 0.286
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (13805 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski