Ruegeria litorea R37

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Ruegeria; Ruegeria litorea

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4492 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y5TNW7|A0A1Y5TNW7_9RHOB UDP-2 3-diacylglucosamine hydrolase OS=Ruegeria litorea R37 OX=1200284 GN=TRL7639_03633 PE=4 SV=1
MM1 pKa = 7.4TRR3 pKa = 11.84FKK5 pKa = 11.04SYY7 pKa = 11.1VLATGLLAGLTAPATAEE24 pKa = 3.94FSFDD28 pKa = 3.76ADD30 pKa = 3.64EE31 pKa = 4.3ATQAYY36 pKa = 9.73VEE38 pKa = 4.46ANLLGIFYY46 pKa = 10.39HH47 pKa = 6.36EE48 pKa = 5.31LGHH51 pKa = 7.57ALIDD55 pKa = 3.88MLDD58 pKa = 3.73LPIFGQEE65 pKa = 3.25EE66 pKa = 3.83DD67 pKa = 3.71AADD70 pKa = 3.74VLSVFLIDD78 pKa = 4.32ALYY81 pKa = 10.27EE82 pKa = 3.98EE83 pKa = 5.36EE84 pKa = 4.44SAVDD88 pKa = 3.74LAYY91 pKa = 9.21GTAFGFLGEE100 pKa = 4.26VEE102 pKa = 3.94QRR104 pKa = 11.84EE105 pKa = 4.52AEE107 pKa = 4.23NEE109 pKa = 3.8LPAYY113 pKa = 9.01WDD115 pKa = 3.15VHH117 pKa = 7.28GPDD120 pKa = 3.16LQRR123 pKa = 11.84YY124 pKa = 7.68FNLVCIFFGANPDD137 pKa = 3.27EE138 pKa = 4.7RR139 pKa = 11.84ADD141 pKa = 3.67VADD144 pKa = 4.99DD145 pKa = 4.2LGLPADD151 pKa = 4.13RR152 pKa = 11.84AEE154 pKa = 4.22YY155 pKa = 10.87CPDD158 pKa = 3.4EE159 pKa = 4.85YY160 pKa = 11.54DD161 pKa = 4.33QADD164 pKa = 4.1HH165 pKa = 6.25SWGVALEE172 pKa = 4.74EE173 pKa = 4.72ILTDD177 pKa = 4.42HH178 pKa = 7.58PAQSIRR184 pKa = 11.84LGSSRR189 pKa = 11.84STPTEE194 pKa = 3.54ATSPPRR200 pKa = 4.32

Molecular weight: 22.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y5RBG9|A0A1Y5RBG9_9RHOB Cytochrome c OS=Ruegeria litorea R37 OX=1200284 GN=TRL7639_00046 PE=4 SV=1
MM1 pKa = 7.76KK2 pKa = 9.8FRR4 pKa = 11.84PMMLINWQSRR14 pKa = 11.84FWRR17 pKa = 11.84KK18 pKa = 8.88PGQRR22 pKa = 11.84GKK24 pKa = 10.75LGAAARR30 pKa = 11.84SLLRR34 pKa = 11.84SRR36 pKa = 11.84KK37 pKa = 9.48NIAAIARR44 pKa = 11.84LIRR47 pKa = 11.84ILKK50 pKa = 9.85SSAIFLILMIPISS63 pKa = 3.62

Molecular weight: 7.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4492

0

4492

1412364

29

3456

314.4

34.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.399 ± 0.046

0.926 ± 0.012

6.208 ± 0.034

5.924 ± 0.032

3.838 ± 0.022

8.575 ± 0.04

2.161 ± 0.018

5.182 ± 0.025

3.426 ± 0.024

9.833 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.864 ± 0.019

2.902 ± 0.023

4.965 ± 0.025

3.532 ± 0.019

6.147 ± 0.035

5.384 ± 0.027

5.602 ± 0.029

7.367 ± 0.029

1.423 ± 0.014

2.342 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski